Journal: eLife

Article Title: Fragile X mental retardation protein is a Zika virus restriction factor that is antagonized by subgenomic flaviviral RNA

doi: 10.7554/eLife.39023

Figure Lengend Snippet: ( A ) Schematic illustrating the secondary structures present in the ZIKV 3′ UTR and RNAs used for affinity chromatography. Deletion mutant RNA constructs fused to a tobramycin aptamer at the 5′ end are shown. DENV NS2A coding sequence was used as a negative control RNA. ( B ) Purified RNAs bound to tobramycin-sepharose beads were incubated with HeLa cell lysate and unbound proteins were washed away prior to elution for western blotting for DDX6, FMRP, FXR1, FXR2, G3BP1 and PTB.

Article Snippet: The following antibodies were used for IP, western blotting and/or immunofluorescence analysis: anti-envelope protein 4G2 , rabbit IgG (2729S, Cell Signaling Technologies), anti-FMRP (ab17722, ABCAM, Cambridge, UK), anti-FXR1 (12295S, Cell Signaling Technologies), anti-FXR2 (7098S, Cell Signaling Technologies), anti-G3BP1 (A302-033A, Bethyl Laboratories), rabbit anti-DDX6 (9407S, Cell Signaling technologies), rabbit anti-PTB (homemade), rabbit anti-ZIKV NS4B (GTX133321, Genetex), anti-ZIKV NS2B (GTX133308, Genetex), anti-BRD4 (13440, Cell Signaling Technologies), anti-GAPDH (ab9485, ABCAM), anti-TLN1 (SC-365875, Santa Cruz Biotechnology), anti-PNPLA6 (SC-271049, Santa Cruz Biotechnology).

Techniques: Affinity Chromatography, Mutagenesis, Construct, Sequencing, Negative Control, Purification, Incubation, Western Blot

Journal: eLife

Article Title: Fragile X mental retardation protein is a Zika virus restriction factor that is antagonized by subgenomic flaviviral RNA

doi: 10.7554/eLife.39023

Figure Lengend Snippet:

Article Snippet: The following antibodies were used for IP, western blotting and/or immunofluorescence analysis: anti-envelope protein 4G2 , rabbit IgG (2729S, Cell Signaling Technologies), anti-FMRP (ab17722, ABCAM, Cambridge, UK), anti-FXR1 (12295S, Cell Signaling Technologies), anti-FXR2 (7098S, Cell Signaling Technologies), anti-G3BP1 (A302-033A, Bethyl Laboratories), rabbit anti-DDX6 (9407S, Cell Signaling technologies), rabbit anti-PTB (homemade), rabbit anti-ZIKV NS4B (GTX133321, Genetex), anti-ZIKV NS2B (GTX133308, Genetex), anti-BRD4 (13440, Cell Signaling Technologies), anti-GAPDH (ab9485, ABCAM), anti-TLN1 (SC-365875, Santa Cruz Biotechnology), anti-PNPLA6 (SC-271049, Santa Cruz Biotechnology).

Techniques: Knock-Out, Sequencing, Northern Blot

Journal: eLife

Article Title: Fragile X mental retardation protein is a Zika virus restriction factor that is antagonized by subgenomic flaviviral RNA

doi: 10.7554/eLife.39023

Figure Lengend Snippet: ( A ) Schematic illustrating the secondary structures present in the ZIKV 3′ UTR and RNAs used for affinity chromatography. Deletion mutant RNA constructs fused to a tobramycin aptamer at the 5′ end are shown. DENV NS2A coding sequence was used as a negative control RNA. ( B ) Purified RNAs bound to tobramycin-sepharose beads were incubated with HeLa cell lysate and unbound proteins were washed away prior to elution for western blotting for DDX6, FMRP, FXR1, FXR2, G3BP1 and PTB.

Article Snippet: Antibody , Anti-DDX6 (Rabbit polyclonal) , Cell Signaling Technology , Cell Signaling Technology Cat# 9407S, RRID: AB_10556959 , (1:1000).

Techniques: Affinity Chromatography, Mutagenesis, Construct, Sequencing, Negative Control, Purification, Incubation, Western Blot

Journal: eLife

Article Title: Fragile X mental retardation protein is a Zika virus restriction factor that is antagonized by subgenomic flaviviral RNA

doi: 10.7554/eLife.39023

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-DDX6 (Rabbit polyclonal) , Cell Signaling Technology , Cell Signaling Technology Cat# 9407S, RRID: AB_10556959 , (1:1000).

Techniques: Knock-Out, Sequencing, Northern Blot

Journal: eLife

Article Title: Fragile X mental retardation protein is a Zika virus restriction factor that is antagonized by subgenomic flaviviral RNA

doi: 10.7554/eLife.39023

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-DDX6 (Rabbit polyclonal) , Cell Signaling Technology , Cell Signaling Technology Cat# 9407S, RRID: AB_10556959 , (1:1000).

Techniques: Knock-Out, Sequencing, Northern Blot