c3h10t1 2 mouse embryonic fibroblast cells  (ATCC)


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    Structured Review

    ATCC c3h10t1 2 mouse embryonic fibroblast cells
    Bio-orthogonal mediated transfection (SnapFect) evaluation and comparison. (A) Fluorescent image of native <t>C3H10T1/2</t> cells with DAPI nuclei stain. (B) Fluorescent image of native C3H10T1/2 cells treated with oxyamine containing DNA-lipoplex. No transfection occurred since the cells did not present ketone groups on their cell surface. (C) Fluorescent image of native CH310T1/2 cells and (D) image of the cells presenting ketone groups exposed to GFP oxyamine lipoplex after 24 h. GFP expression resulted due to specific bio-orthogonal mediated transfection. (E) Representative image of RFP transfected fibroblast cells via click chemistry mediated transfection. (F) A protein gel showing that the expression of RFP is mediated by the correct pairing of the bio-orthogonal chemistry functional groups on the cell surface and on the DNA-lipoplex. O+ and K+ represent oxyamine containing lipoplexes (O+) and ketone presenting cells (K+) used for RFP transfection. O– and K– represent conditions where either oxyamine or ketone was not present on the cell surface or DNA-lipoplex. Only the correct combination resulted in bio-orthogonal mediated transfection. These experiments were performed over 10 times and showed similar results. (G, H) Luciferase transfection assay for fibroblasts using the bio-orthogonal mediated transfection strategy. (H) Comparison of luciferase assay transfection for the bio-orthogonal mediated strategy (SnapFect) and leading commercial transfection reagents. The luciferase assays were replicated over 10 times and averaged ( p
    C3h10t1 2 Mouse Embryonic Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3h10t1 2 mouse embryonic fibroblast cells/product/ATCC
    Average 97 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    c3h10t1 2 mouse embryonic fibroblast cells - by Bioz Stars, 2022-09
    97/100 stars

    Images

    1) Product Images from "Bio-Orthogonal Mediated Nucleic Acid Transfection of Cells via Cell Surface Engineering"

    Article Title: Bio-Orthogonal Mediated Nucleic Acid Transfection of Cells via Cell Surface Engineering

    Journal: ACS Central Science

    doi: 10.1021/acscentsci.7b00132

    Bio-orthogonal mediated transfection (SnapFect) evaluation and comparison. (A) Fluorescent image of native C3H10T1/2 cells with DAPI nuclei stain. (B) Fluorescent image of native C3H10T1/2 cells treated with oxyamine containing DNA-lipoplex. No transfection occurred since the cells did not present ketone groups on their cell surface. (C) Fluorescent image of native CH310T1/2 cells and (D) image of the cells presenting ketone groups exposed to GFP oxyamine lipoplex after 24 h. GFP expression resulted due to specific bio-orthogonal mediated transfection. (E) Representative image of RFP transfected fibroblast cells via click chemistry mediated transfection. (F) A protein gel showing that the expression of RFP is mediated by the correct pairing of the bio-orthogonal chemistry functional groups on the cell surface and on the DNA-lipoplex. O+ and K+ represent oxyamine containing lipoplexes (O+) and ketone presenting cells (K+) used for RFP transfection. O– and K– represent conditions where either oxyamine or ketone was not present on the cell surface or DNA-lipoplex. Only the correct combination resulted in bio-orthogonal mediated transfection. These experiments were performed over 10 times and showed similar results. (G, H) Luciferase transfection assay for fibroblasts using the bio-orthogonal mediated transfection strategy. (H) Comparison of luciferase assay transfection for the bio-orthogonal mediated strategy (SnapFect) and leading commercial transfection reagents. The luciferase assays were replicated over 10 times and averaged ( p
    Figure Legend Snippet: Bio-orthogonal mediated transfection (SnapFect) evaluation and comparison. (A) Fluorescent image of native C3H10T1/2 cells with DAPI nuclei stain. (B) Fluorescent image of native C3H10T1/2 cells treated with oxyamine containing DNA-lipoplex. No transfection occurred since the cells did not present ketone groups on their cell surface. (C) Fluorescent image of native CH310T1/2 cells and (D) image of the cells presenting ketone groups exposed to GFP oxyamine lipoplex after 24 h. GFP expression resulted due to specific bio-orthogonal mediated transfection. (E) Representative image of RFP transfected fibroblast cells via click chemistry mediated transfection. (F) A protein gel showing that the expression of RFP is mediated by the correct pairing of the bio-orthogonal chemistry functional groups on the cell surface and on the DNA-lipoplex. O+ and K+ represent oxyamine containing lipoplexes (O+) and ketone presenting cells (K+) used for RFP transfection. O– and K– represent conditions where either oxyamine or ketone was not present on the cell surface or DNA-lipoplex. Only the correct combination resulted in bio-orthogonal mediated transfection. These experiments were performed over 10 times and showed similar results. (G, H) Luciferase transfection assay for fibroblasts using the bio-orthogonal mediated transfection strategy. (H) Comparison of luciferase assay transfection for the bio-orthogonal mediated strategy (SnapFect) and leading commercial transfection reagents. The luciferase assays were replicated over 10 times and averaged ( p

    Techniques Used: Transfection, Staining, Expressing, Functional Assay, Luciferase

    Precision transfection via bio-orthogonal mediated ligation in cocultures. (Left) RFP expressing HDNF cells (K) are cell surface engineered to present ketone groups (L) via rapid liposome fusion (J). Nonfluorescent C3H10T/1/2 cells and ketone presenting HDNF cells (L) are mixed to generate a coculture (N). To this coculture a bio-orthogonal oxyamine presenting GFP-lipoplex (X) is added. Only the ketone presenting cells (L) are targeted for selective transfection via the oxyamine presenting lipoplex (X) via oxime ligation. (O) Fluorescent image showing that only the HDNF presenting ketones are transfected with GFP and are yellow. (Right) Nonfluorescent C3H10T1/2 cells (M) are cell surface engineered to present ketone groups (P) via rapid liposome fusion (J). Red fluorescent HDNF cells (K) and ketone presenting C3H10T1/2 cells (P) are mixed to generate a coculture (Q). To this coculture a bio-orthogonal oxyamine presenting GFP-lipoplex (X) is added. Only the ketone presenting cells (P) are targeted for selective transfection via the oxyamine presenting lipoplex (X) via oxime ligation. (R) Fluorescent image showing that only the CH310T1/2 cells presenting ketones are transfected with GFP and turn green. The experiments were replicated over 20 times with similar results as determined by fluorescence imaging and protein gel analysis. These coculture results show that transfection is only mediated through targeted bio-orthogonal chemistry and not nonspecific electrostatic interactions.
    Figure Legend Snippet: Precision transfection via bio-orthogonal mediated ligation in cocultures. (Left) RFP expressing HDNF cells (K) are cell surface engineered to present ketone groups (L) via rapid liposome fusion (J). Nonfluorescent C3H10T/1/2 cells and ketone presenting HDNF cells (L) are mixed to generate a coculture (N). To this coculture a bio-orthogonal oxyamine presenting GFP-lipoplex (X) is added. Only the ketone presenting cells (L) are targeted for selective transfection via the oxyamine presenting lipoplex (X) via oxime ligation. (O) Fluorescent image showing that only the HDNF presenting ketones are transfected with GFP and are yellow. (Right) Nonfluorescent C3H10T1/2 cells (M) are cell surface engineered to present ketone groups (P) via rapid liposome fusion (J). Red fluorescent HDNF cells (K) and ketone presenting C3H10T1/2 cells (P) are mixed to generate a coculture (Q). To this coculture a bio-orthogonal oxyamine presenting GFP-lipoplex (X) is added. Only the ketone presenting cells (P) are targeted for selective transfection via the oxyamine presenting lipoplex (X) via oxime ligation. (R) Fluorescent image showing that only the CH310T1/2 cells presenting ketones are transfected with GFP and turn green. The experiments were replicated over 20 times with similar results as determined by fluorescence imaging and protein gel analysis. These coculture results show that transfection is only mediated through targeted bio-orthogonal chemistry and not nonspecific electrostatic interactions.

    Techniques Used: Transfection, Ligation, Expressing, Fluorescence, Imaging

    2) Product Images from "Peripheral Aβ acts as a negative modulator of insulin secretion"

    Article Title: Peripheral Aβ acts as a negative modulator of insulin secretion

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2117723119

    In vitro effects of glucose and insulin loading on Aβ secretion and of Aβ loading on insulin and GLP-1 secretion and glucose uptake in cell culture. All cells were cultured in no-glucose medium for 120 min prior to the glucose and insulin stimulation. ( A ) β-TC-6 cells (islet β-cells) were stimulated with high-glucose for 60 min in the presence or absence of GLP-1. High-glucose stimulation caused a significant increase in Aβ40, Aβ42, and insulin levels in the media, which was further enhanced by GLP-1. ( B ) β-TC-6 cells were stimulated with high glucose for 60 min in the presence or absence of Aβ40. Aβ40 significantly inhibited the glucose-induced insulin secretion. ( C ) β-TC-6 cells were stimulated with high glucose in the presence or absence of a γ-secretase inhibitor, L685,458. L685,458 significantly enhanced both the steady state and glucose-induced insulin secretion. Differentiated 3T3-L1 cells (adipocytes) ( D ), differentiated C2C12 cells (myocytes) ( E ), and FL83B cells (hepatocytes) ( F ) were stimulated with high glucose for 60 min with or without insulin. High-glucose stimulation did not affect Aβ40 or Aβ42 secretion, but insulin stimulation significantly enhanced it in these cells. ( G ) NCI-H716 cells were stimulated with high glucose for 120 min in the presence or absence of Aβ40 or Aβ42. High-glucose stimulation caused a significant increase in the GLP-1 level in the media, while the addition of Aβ40 and Aβ42 had no effect. ( H ) Differentiated 3T3-L1 cells were stimulated with insulin in the presence or absence of Aβ40 or Aβ42 for 20 min. The cells were further incubated for 10 min in the presence of 3 H-labeled glucose. Insulin stimulation significantly increased the glucose uptake, but the addition of Aβ40 or Aβ42 had no effect.
    Figure Legend Snippet: In vitro effects of glucose and insulin loading on Aβ secretion and of Aβ loading on insulin and GLP-1 secretion and glucose uptake in cell culture. All cells were cultured in no-glucose medium for 120 min prior to the glucose and insulin stimulation. ( A ) β-TC-6 cells (islet β-cells) were stimulated with high-glucose for 60 min in the presence or absence of GLP-1. High-glucose stimulation caused a significant increase in Aβ40, Aβ42, and insulin levels in the media, which was further enhanced by GLP-1. ( B ) β-TC-6 cells were stimulated with high glucose for 60 min in the presence or absence of Aβ40. Aβ40 significantly inhibited the glucose-induced insulin secretion. ( C ) β-TC-6 cells were stimulated with high glucose in the presence or absence of a γ-secretase inhibitor, L685,458. L685,458 significantly enhanced both the steady state and glucose-induced insulin secretion. Differentiated 3T3-L1 cells (adipocytes) ( D ), differentiated C2C12 cells (myocytes) ( E ), and FL83B cells (hepatocytes) ( F ) were stimulated with high glucose for 60 min with or without insulin. High-glucose stimulation did not affect Aβ40 or Aβ42 secretion, but insulin stimulation significantly enhanced it in these cells. ( G ) NCI-H716 cells were stimulated with high glucose for 120 min in the presence or absence of Aβ40 or Aβ42. High-glucose stimulation caused a significant increase in the GLP-1 level in the media, while the addition of Aβ40 and Aβ42 had no effect. ( H ) Differentiated 3T3-L1 cells were stimulated with insulin in the presence or absence of Aβ40 or Aβ42 for 20 min. The cells were further incubated for 10 min in the presence of 3 H-labeled glucose. Insulin stimulation significantly increased the glucose uptake, but the addition of Aβ40 or Aβ42 had no effect.

    Techniques Used: In Vitro, Cell Culture, Incubation, Labeling

    3) Product Images from "Collagen triple helix repeat containing 1 is a new promigratory marker of arthritic pannus"

    Article Title: Collagen triple helix repeat containing 1 is a new promigratory marker of arthritic pannus

    Journal: Arthritis Research & Therapy

    doi: 10.1186/s13075-016-1067-1

    Collagen triple helix repeat containing 1 ( CTHRC1 ) effects transwell cell migration. (a) Western immunoblotting detection of CTHRC1 in rheumatoid arthritis-fibroblast-like synoviocytes ( RA-FLS ), NIH 3T3, C3H/10 T1/2. RA-FLS express CTHRC1, but murine fibroblasts are negative for CTHRC1. (b) RA-FLS also express cadherin CDH11, alpha smooth muscle actin ( α-SMA ), and α-tubulin. Staining membrane with secondary antibodies alone was negative. (c) CTHRC1 is secreted into conditioned media upon transfection and overexpression in Chinese hamster ovary (CHO) cells. Lanes : (1) positive control 10 ng rhCTHRC1; (2) day 0; (3) day 1; (4) day 3; (5) day 5. (d) RA-FLS were treated directly in transwell chambers with rhCTHRC1 protein, while FBS concentration was varied from 0 %, 1–6 %. Cells that migrated through the membrane were stained with toluidine blue and counted under the microscope. Solid black columns represent cells treated with rhCTHRC1, gray columns are untreated cells. (e) murine C3H/10T1/2 fibroblasts were treated with rhCTHRC1 similarly to RA-FLS. (f) Serum from mice with acute CAIA (5 % serum mixed with 5 % FBS in complete media) showed the strongest promigratory effect. (g) Toluidine blue staining and microscopy of cells migrated through the membrane and spread on its opposite side after the completion of the experiment. Numerous dots are membrane pores of 8 μm in size. Data are representative of three similar but independent experiments. Statistical significance was calculated using Student’s t test: * p
    Figure Legend Snippet: Collagen triple helix repeat containing 1 ( CTHRC1 ) effects transwell cell migration. (a) Western immunoblotting detection of CTHRC1 in rheumatoid arthritis-fibroblast-like synoviocytes ( RA-FLS ), NIH 3T3, C3H/10 T1/2. RA-FLS express CTHRC1, but murine fibroblasts are negative for CTHRC1. (b) RA-FLS also express cadherin CDH11, alpha smooth muscle actin ( α-SMA ), and α-tubulin. Staining membrane with secondary antibodies alone was negative. (c) CTHRC1 is secreted into conditioned media upon transfection and overexpression in Chinese hamster ovary (CHO) cells. Lanes : (1) positive control 10 ng rhCTHRC1; (2) day 0; (3) day 1; (4) day 3; (5) day 5. (d) RA-FLS were treated directly in transwell chambers with rhCTHRC1 protein, while FBS concentration was varied from 0 %, 1–6 %. Cells that migrated through the membrane were stained with toluidine blue and counted under the microscope. Solid black columns represent cells treated with rhCTHRC1, gray columns are untreated cells. (e) murine C3H/10T1/2 fibroblasts were treated with rhCTHRC1 similarly to RA-FLS. (f) Serum from mice with acute CAIA (5 % serum mixed with 5 % FBS in complete media) showed the strongest promigratory effect. (g) Toluidine blue staining and microscopy of cells migrated through the membrane and spread on its opposite side after the completion of the experiment. Numerous dots are membrane pores of 8 μm in size. Data are representative of three similar but independent experiments. Statistical significance was calculated using Student’s t test: * p

    Techniques Used: Migration, Western Blot, Staining, Transfection, Over Expression, Positive Control, Concentration Assay, Microscopy, Mouse Assay

    4) Product Images from "Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes: A novel fluorescent method to quantify fat droplets"

    Article Title: Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes: A novel fluorescent method to quantify fat droplets

    Journal: Nutrition & Metabolism

    doi: 10.1186/1743-7075-8-30

    Summary of overall effects of the dietary ingredients DHA, β-carotene and resveratrol on lipid accumulation and on gene expression clusters . mRNA levels of enzymes involved in glucose and fat metabolism (Glut4, LPL, FAS, ACC1 and CPT-1β) and of adipocyte differentiation markers (PPARγ, C/EBPα, PPARα, aP2 and adiponectin) were determined in maturing C3H10 T1/2 cells. Depicted are the dose-dependent effects of DHA and β-carotene and the impact of resveratrol on lipid accumulation (after 7 days treatment, top of the table) and on gene expression (after 4 days treatment) relative to rosiglitazone control cells. Data are shown as % of positive control ± SEM (fat accumulation parameters) and fold change ± error (based on SEM, n = 6 - 15) for gene expression levels, respectively. Exemplarily, the fold changes of the genes FAS and PPARγ are shown as illustration. Fat droplet number equates to Spot Count/Object; fat droplet intensity equates to Spot Avg Intensity and fat droplet area equates to Spot Total Area/Object. Student's t-test: treatment versus control (*) p
    Figure Legend Snippet: Summary of overall effects of the dietary ingredients DHA, β-carotene and resveratrol on lipid accumulation and on gene expression clusters . mRNA levels of enzymes involved in glucose and fat metabolism (Glut4, LPL, FAS, ACC1 and CPT-1β) and of adipocyte differentiation markers (PPARγ, C/EBPα, PPARα, aP2 and adiponectin) were determined in maturing C3H10 T1/2 cells. Depicted are the dose-dependent effects of DHA and β-carotene and the impact of resveratrol on lipid accumulation (after 7 days treatment, top of the table) and on gene expression (after 4 days treatment) relative to rosiglitazone control cells. Data are shown as % of positive control ± SEM (fat accumulation parameters) and fold change ± error (based on SEM, n = 6 - 15) for gene expression levels, respectively. Exemplarily, the fold changes of the genes FAS and PPARγ are shown as illustration. Fat droplet number equates to Spot Count/Object; fat droplet intensity equates to Spot Avg Intensity and fat droplet area equates to Spot Total Area/Object. Student's t-test: treatment versus control (*) p

    Techniques Used: Expressing, Cycling Probe Technology, Positive Control

    Visualisation of lipid accumulation in differentiated adipocytes . C3H10 T1/2 cells were treated for 7 days with rosiglitazone (0.01, 0.1, 1 and 10 μM) and, after fixation with 60% isopropanol, stained with A) Oil Red O or B) Hoechst 33342 and BODIPY ® 493/503. Images were acquired using A) a Nikon Coolpix 990 camera at 20× magnification or B) the Cellomics ® HCS Reader camera (20×).
    Figure Legend Snippet: Visualisation of lipid accumulation in differentiated adipocytes . C3H10 T1/2 cells were treated for 7 days with rosiglitazone (0.01, 0.1, 1 and 10 μM) and, after fixation with 60% isopropanol, stained with A) Oil Red O or B) Hoechst 33342 and BODIPY ® 493/503. Images were acquired using A) a Nikon Coolpix 990 camera at 20× magnification or B) the Cellomics ® HCS Reader camera (20×).

    Techniques Used: Staining

    Effects of DHA, EPA, β-carotene, HT, (all-E)-lycopene, EGCG and resveratrol on lipid formation measured with the HCA assay . C3H10 T1/2 cells were treated for 7 days. Shown are three parameters (Spot Count/Object, Spot Avg Intensity and Spot Total Area/Object) that quantify lipid formation, as compared to rosiglitazone controls set as 100%. Data are shown as mean ± SEM (n = 10 - 20). Student's t-test: treatment versus control (*) p
    Figure Legend Snippet: Effects of DHA, EPA, β-carotene, HT, (all-E)-lycopene, EGCG and resveratrol on lipid formation measured with the HCA assay . C3H10 T1/2 cells were treated for 7 days. Shown are three parameters (Spot Count/Object, Spot Avg Intensity and Spot Total Area/Object) that quantify lipid formation, as compared to rosiglitazone controls set as 100%. Data are shown as mean ± SEM (n = 10 - 20). Student's t-test: treatment versus control (*) p

    Techniques Used: High Content Screening

    Effects of varying concentrations of DHA, EPA, β-carotene, (all-E)-lycopene, resveratrol and EGCG on number and intensity of fat droplets . C3H10 T1/2 cells were differentiated in the presence of test substances for 7 days. Spot Count/Object and Spot Avg Intensity were determined, as compared to rosiglitazone control set as 100%. Data are shown as mean ± SEM (n = 10 - 20). Student's t-test: treatment versus control (*) p
    Figure Legend Snippet: Effects of varying concentrations of DHA, EPA, β-carotene, (all-E)-lycopene, resveratrol and EGCG on number and intensity of fat droplets . C3H10 T1/2 cells were differentiated in the presence of test substances for 7 days. Spot Count/Object and Spot Avg Intensity were determined, as compared to rosiglitazone control set as 100%. Data are shown as mean ± SEM (n = 10 - 20). Student's t-test: treatment versus control (*) p

    Techniques Used:

    5) Product Images from "Activation of AMP-Activated Protein Kinase-Sirtuin 1 Pathway Contributes to Salvianolic Acid A-Induced Browning of White Adipose Tissue in High-Fat Diet Fed Male Mice"

    Article Title: Activation of AMP-Activated Protein Kinase-Sirtuin 1 Pathway Contributes to Salvianolic Acid A-Induced Browning of White Adipose Tissue in High-Fat Diet Fed Male Mice

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.614406

    AMPK activation contributes to Sal A-induced UCP-1 upregulation. (A) Differentiated C3H10T1/2 adipocytes were treated with Sal A (0, 20, 40, and 80 μM) for 4 h, intracellular p -PKA, PKA, p-p38, p38, p -AMPK, and AMPK contents were determined by Western blot. (B) Fully-differentiated C3H10T1/2 cells were pre-treated with PKA inhibitor (H89, 20 μM), p38 inhibitor (SB203580, 10 μM), and AMPK inhibitor (compound C, 1 μM), respectively, for 2 h before incubated with Sal A (80 μM) for 4 h. Intracellular UCP-1 mRNA level was measured by RT-PCR. (C) Fully-differentiated C3H10T1/2 adipocytes were transfected with siRNAs for AMPK. After silencing AMPK by siRNA, cells were exposed to 80 μM Sal A for 4 h. The protein levels of UCP1, AMPK, p -ACC and ACC were detected by Western blot. All values are denoted as means ± SD from three independent batches of cells. # p
    Figure Legend Snippet: AMPK activation contributes to Sal A-induced UCP-1 upregulation. (A) Differentiated C3H10T1/2 adipocytes were treated with Sal A (0, 20, 40, and 80 μM) for 4 h, intracellular p -PKA, PKA, p-p38, p38, p -AMPK, and AMPK contents were determined by Western blot. (B) Fully-differentiated C3H10T1/2 cells were pre-treated with PKA inhibitor (H89, 20 μM), p38 inhibitor (SB203580, 10 μM), and AMPK inhibitor (compound C, 1 μM), respectively, for 2 h before incubated with Sal A (80 μM) for 4 h. Intracellular UCP-1 mRNA level was measured by RT-PCR. (C) Fully-differentiated C3H10T1/2 adipocytes were transfected with siRNAs for AMPK. After silencing AMPK by siRNA, cells were exposed to 80 μM Sal A for 4 h. The protein levels of UCP1, AMPK, p -ACC and ACC were detected by Western blot. All values are denoted as means ± SD from three independent batches of cells. # p

    Techniques Used: Activation Assay, Western Blot, Incubation, Reverse Transcription Polymerase Chain Reaction, Transfection

    Sal A treatment induced UCP-1 expression in adipocytes. (A,B) Differentiated C3H10T1/2 adipocytes were treated with Sal A (0, 20, 40, and 80 μM) for 4 h, intracellular UCP-1 content was determined by RT-PCR and Western blot, respectively. (C,D) Time-course changes of intracellular mRNA and protein levels of UCP-1 were determined on Sal A (80 μM) treatment with different duration (0, 2, 4, and 8 h). All values are denoted as means ± SD from three independent batches of cells. # p
    Figure Legend Snippet: Sal A treatment induced UCP-1 expression in adipocytes. (A,B) Differentiated C3H10T1/2 adipocytes were treated with Sal A (0, 20, 40, and 80 μM) for 4 h, intracellular UCP-1 content was determined by RT-PCR and Western blot, respectively. (C,D) Time-course changes of intracellular mRNA and protein levels of UCP-1 were determined on Sal A (80 μM) treatment with different duration (0, 2, 4, and 8 h). All values are denoted as means ± SD from three independent batches of cells. # p

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    SIRT1 increment underlies Sal A-induced AMPK activation and UCP-1 upregulation. (A) Differentiated C3H10T1/2 adipocytes were treated with Sal A (80 μM) for 0, 2, 4 and 8 h. Intracellular SIRT1 contents were determined by Western blot. (B) After silencing SIRT1 by siRNA, cells were exposed to 80 μM Sal A for 4 h. The protein levels of UCP-1 and SIRT1 were detected by Western blot. (C) C3H10T1/2 adipocytes were transfected with siRNA for SIRT1 or AMPK, respectively. Then cells were treated with Sal A (80 μM) for 4 h. Total cellular lysates were collected for the immunoblotting assay for SIRT1, p -AMPK and AMPK. All values are denoted as means ± SD from three independent batches of cells. # p
    Figure Legend Snippet: SIRT1 increment underlies Sal A-induced AMPK activation and UCP-1 upregulation. (A) Differentiated C3H10T1/2 adipocytes were treated with Sal A (80 μM) for 0, 2, 4 and 8 h. Intracellular SIRT1 contents were determined by Western blot. (B) After silencing SIRT1 by siRNA, cells were exposed to 80 μM Sal A for 4 h. The protein levels of UCP-1 and SIRT1 were detected by Western blot. (C) C3H10T1/2 adipocytes were transfected with siRNA for SIRT1 or AMPK, respectively. Then cells were treated with Sal A (80 μM) for 4 h. Total cellular lysates were collected for the immunoblotting assay for SIRT1, p -AMPK and AMPK. All values are denoted as means ± SD from three independent batches of cells. # p

    Techniques Used: Activation Assay, Western Blot, Transfection

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    ATCC c3h10t1 2 mouse embryonic fibroblast cells
    Bio-orthogonal mediated transfection (SnapFect) evaluation and comparison. (A) Fluorescent image of native <t>C3H10T1/2</t> cells with DAPI nuclei stain. (B) Fluorescent image of native C3H10T1/2 cells treated with oxyamine containing DNA-lipoplex. No transfection occurred since the cells did not present ketone groups on their cell surface. (C) Fluorescent image of native CH310T1/2 cells and (D) image of the cells presenting ketone groups exposed to GFP oxyamine lipoplex after 24 h. GFP expression resulted due to specific bio-orthogonal mediated transfection. (E) Representative image of RFP transfected fibroblast cells via click chemistry mediated transfection. (F) A protein gel showing that the expression of RFP is mediated by the correct pairing of the bio-orthogonal chemistry functional groups on the cell surface and on the DNA-lipoplex. O+ and K+ represent oxyamine containing lipoplexes (O+) and ketone presenting cells (K+) used for RFP transfection. O– and K– represent conditions where either oxyamine or ketone was not present on the cell surface or DNA-lipoplex. Only the correct combination resulted in bio-orthogonal mediated transfection. These experiments were performed over 10 times and showed similar results. (G, H) Luciferase transfection assay for fibroblasts using the bio-orthogonal mediated transfection strategy. (H) Comparison of luciferase assay transfection for the bio-orthogonal mediated strategy (SnapFect) and leading commercial transfection reagents. The luciferase assays were replicated over 10 times and averaged ( p
    C3h10t1 2 Mouse Embryonic Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3h10t1 2 mouse embryonic fibroblast cells/product/ATCC
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    c3h10t1 2 mouse embryonic fibroblast cells - by Bioz Stars, 2022-09
    97/100 stars
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    Bio-orthogonal mediated transfection (SnapFect) evaluation and comparison. (A) Fluorescent image of native C3H10T1/2 cells with DAPI nuclei stain. (B) Fluorescent image of native C3H10T1/2 cells treated with oxyamine containing DNA-lipoplex. No transfection occurred since the cells did not present ketone groups on their cell surface. (C) Fluorescent image of native CH310T1/2 cells and (D) image of the cells presenting ketone groups exposed to GFP oxyamine lipoplex after 24 h. GFP expression resulted due to specific bio-orthogonal mediated transfection. (E) Representative image of RFP transfected fibroblast cells via click chemistry mediated transfection. (F) A protein gel showing that the expression of RFP is mediated by the correct pairing of the bio-orthogonal chemistry functional groups on the cell surface and on the DNA-lipoplex. O+ and K+ represent oxyamine containing lipoplexes (O+) and ketone presenting cells (K+) used for RFP transfection. O– and K– represent conditions where either oxyamine or ketone was not present on the cell surface or DNA-lipoplex. Only the correct combination resulted in bio-orthogonal mediated transfection. These experiments were performed over 10 times and showed similar results. (G, H) Luciferase transfection assay for fibroblasts using the bio-orthogonal mediated transfection strategy. (H) Comparison of luciferase assay transfection for the bio-orthogonal mediated strategy (SnapFect) and leading commercial transfection reagents. The luciferase assays were replicated over 10 times and averaged ( p

    Journal: ACS Central Science

    Article Title: Bio-Orthogonal Mediated Nucleic Acid Transfection of Cells via Cell Surface Engineering

    doi: 10.1021/acscentsci.7b00132

    Figure Lengend Snippet: Bio-orthogonal mediated transfection (SnapFect) evaluation and comparison. (A) Fluorescent image of native C3H10T1/2 cells with DAPI nuclei stain. (B) Fluorescent image of native C3H10T1/2 cells treated with oxyamine containing DNA-lipoplex. No transfection occurred since the cells did not present ketone groups on their cell surface. (C) Fluorescent image of native CH310T1/2 cells and (D) image of the cells presenting ketone groups exposed to GFP oxyamine lipoplex after 24 h. GFP expression resulted due to specific bio-orthogonal mediated transfection. (E) Representative image of RFP transfected fibroblast cells via click chemistry mediated transfection. (F) A protein gel showing that the expression of RFP is mediated by the correct pairing of the bio-orthogonal chemistry functional groups on the cell surface and on the DNA-lipoplex. O+ and K+ represent oxyamine containing lipoplexes (O+) and ketone presenting cells (K+) used for RFP transfection. O– and K– represent conditions where either oxyamine or ketone was not present on the cell surface or DNA-lipoplex. Only the correct combination resulted in bio-orthogonal mediated transfection. These experiments were performed over 10 times and showed similar results. (G, H) Luciferase transfection assay for fibroblasts using the bio-orthogonal mediated transfection strategy. (H) Comparison of luciferase assay transfection for the bio-orthogonal mediated strategy (SnapFect) and leading commercial transfection reagents. The luciferase assays were replicated over 10 times and averaged ( p

    Article Snippet: Cellular Culture C3H10T1/2 For our model system we used C3H10T1/2 mouse embryonic fibroblast cells (ATCC) which were cultured and incubated for 3 days at 37 °C and 5% CO2 in 10 cm culture plates (Fisher Scientific) with media changed every other day using DMEM (Sigma-Aldrich) with 1% v/v PS (Sigma-Aldrich) and 10% FBS (Sigma-Aldrich) as additives.

    Techniques: Transfection, Staining, Expressing, Functional Assay, Luciferase

    Precision transfection via bio-orthogonal mediated ligation in cocultures. (Left) RFP expressing HDNF cells (K) are cell surface engineered to present ketone groups (L) via rapid liposome fusion (J). Nonfluorescent C3H10T/1/2 cells and ketone presenting HDNF cells (L) are mixed to generate a coculture (N). To this coculture a bio-orthogonal oxyamine presenting GFP-lipoplex (X) is added. Only the ketone presenting cells (L) are targeted for selective transfection via the oxyamine presenting lipoplex (X) via oxime ligation. (O) Fluorescent image showing that only the HDNF presenting ketones are transfected with GFP and are yellow. (Right) Nonfluorescent C3H10T1/2 cells (M) are cell surface engineered to present ketone groups (P) via rapid liposome fusion (J). Red fluorescent HDNF cells (K) and ketone presenting C3H10T1/2 cells (P) are mixed to generate a coculture (Q). To this coculture a bio-orthogonal oxyamine presenting GFP-lipoplex (X) is added. Only the ketone presenting cells (P) are targeted for selective transfection via the oxyamine presenting lipoplex (X) via oxime ligation. (R) Fluorescent image showing that only the CH310T1/2 cells presenting ketones are transfected with GFP and turn green. The experiments were replicated over 20 times with similar results as determined by fluorescence imaging and protein gel analysis. These coculture results show that transfection is only mediated through targeted bio-orthogonal chemistry and not nonspecific electrostatic interactions.

    Journal: ACS Central Science

    Article Title: Bio-Orthogonal Mediated Nucleic Acid Transfection of Cells via Cell Surface Engineering

    doi: 10.1021/acscentsci.7b00132

    Figure Lengend Snippet: Precision transfection via bio-orthogonal mediated ligation in cocultures. (Left) RFP expressing HDNF cells (K) are cell surface engineered to present ketone groups (L) via rapid liposome fusion (J). Nonfluorescent C3H10T/1/2 cells and ketone presenting HDNF cells (L) are mixed to generate a coculture (N). To this coculture a bio-orthogonal oxyamine presenting GFP-lipoplex (X) is added. Only the ketone presenting cells (L) are targeted for selective transfection via the oxyamine presenting lipoplex (X) via oxime ligation. (O) Fluorescent image showing that only the HDNF presenting ketones are transfected with GFP and are yellow. (Right) Nonfluorescent C3H10T1/2 cells (M) are cell surface engineered to present ketone groups (P) via rapid liposome fusion (J). Red fluorescent HDNF cells (K) and ketone presenting C3H10T1/2 cells (P) are mixed to generate a coculture (Q). To this coculture a bio-orthogonal oxyamine presenting GFP-lipoplex (X) is added. Only the ketone presenting cells (P) are targeted for selective transfection via the oxyamine presenting lipoplex (X) via oxime ligation. (R) Fluorescent image showing that only the CH310T1/2 cells presenting ketones are transfected with GFP and turn green. The experiments were replicated over 20 times with similar results as determined by fluorescence imaging and protein gel analysis. These coculture results show that transfection is only mediated through targeted bio-orthogonal chemistry and not nonspecific electrostatic interactions.

    Article Snippet: Cellular Culture C3H10T1/2 For our model system we used C3H10T1/2 mouse embryonic fibroblast cells (ATCC) which were cultured and incubated for 3 days at 37 °C and 5% CO2 in 10 cm culture plates (Fisher Scientific) with media changed every other day using DMEM (Sigma-Aldrich) with 1% v/v PS (Sigma-Aldrich) and 10% FBS (Sigma-Aldrich) as additives.

    Techniques: Transfection, Ligation, Expressing, Fluorescence, Imaging

    In vitro effects of glucose and insulin loading on Aβ secretion and of Aβ loading on insulin and GLP-1 secretion and glucose uptake in cell culture. All cells were cultured in no-glucose medium for 120 min prior to the glucose and insulin stimulation. ( A ) β-TC-6 cells (islet β-cells) were stimulated with high-glucose for 60 min in the presence or absence of GLP-1. High-glucose stimulation caused a significant increase in Aβ40, Aβ42, and insulin levels in the media, which was further enhanced by GLP-1. ( B ) β-TC-6 cells were stimulated with high glucose for 60 min in the presence or absence of Aβ40. Aβ40 significantly inhibited the glucose-induced insulin secretion. ( C ) β-TC-6 cells were stimulated with high glucose in the presence or absence of a γ-secretase inhibitor, L685,458. L685,458 significantly enhanced both the steady state and glucose-induced insulin secretion. Differentiated 3T3-L1 cells (adipocytes) ( D ), differentiated C2C12 cells (myocytes) ( E ), and FL83B cells (hepatocytes) ( F ) were stimulated with high glucose for 60 min with or without insulin. High-glucose stimulation did not affect Aβ40 or Aβ42 secretion, but insulin stimulation significantly enhanced it in these cells. ( G ) NCI-H716 cells were stimulated with high glucose for 120 min in the presence or absence of Aβ40 or Aβ42. High-glucose stimulation caused a significant increase in the GLP-1 level in the media, while the addition of Aβ40 and Aβ42 had no effect. ( H ) Differentiated 3T3-L1 cells were stimulated with insulin in the presence or absence of Aβ40 or Aβ42 for 20 min. The cells were further incubated for 10 min in the presence of 3 H-labeled glucose. Insulin stimulation significantly increased the glucose uptake, but the addition of Aβ40 or Aβ42 had no effect.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Peripheral Aβ acts as a negative modulator of insulin secretion

    doi: 10.1073/pnas.2117723119

    Figure Lengend Snippet: In vitro effects of glucose and insulin loading on Aβ secretion and of Aβ loading on insulin and GLP-1 secretion and glucose uptake in cell culture. All cells were cultured in no-glucose medium for 120 min prior to the glucose and insulin stimulation. ( A ) β-TC-6 cells (islet β-cells) were stimulated with high-glucose for 60 min in the presence or absence of GLP-1. High-glucose stimulation caused a significant increase in Aβ40, Aβ42, and insulin levels in the media, which was further enhanced by GLP-1. ( B ) β-TC-6 cells were stimulated with high glucose for 60 min in the presence or absence of Aβ40. Aβ40 significantly inhibited the glucose-induced insulin secretion. ( C ) β-TC-6 cells were stimulated with high glucose in the presence or absence of a γ-secretase inhibitor, L685,458. L685,458 significantly enhanced both the steady state and glucose-induced insulin secretion. Differentiated 3T3-L1 cells (adipocytes) ( D ), differentiated C2C12 cells (myocytes) ( E ), and FL83B cells (hepatocytes) ( F ) were stimulated with high glucose for 60 min with or without insulin. High-glucose stimulation did not affect Aβ40 or Aβ42 secretion, but insulin stimulation significantly enhanced it in these cells. ( G ) NCI-H716 cells were stimulated with high glucose for 120 min in the presence or absence of Aβ40 or Aβ42. High-glucose stimulation caused a significant increase in the GLP-1 level in the media, while the addition of Aβ40 and Aβ42 had no effect. ( H ) Differentiated 3T3-L1 cells were stimulated with insulin in the presence or absence of Aβ40 or Aβ42 for 20 min. The cells were further incubated for 10 min in the presence of 3 H-labeled glucose. Insulin stimulation significantly increased the glucose uptake, but the addition of Aβ40 or Aβ42 had no effect.

    Article Snippet: The mouse pancreatic β-cell line β-TC-6, mouse embryonic fibroblast line 3T3-L1, mouse myoblast line C2C12, and mouse hepatocyte line FL83B were obtained from the ATCC/American Type Culture Collection. β-TC-6, 3T3-L1, and C2C12 cells were cultured in low-glucose DMEM (Fujifilm-Wako) supplemented with 15% fetal bovine serum (FBS), 10% bovine serum, and 10% FBS, respectively, while FL83B cells were maintained in Kaighn’s Modification of Ham’s F-12 medium containing 10% FBS.

    Techniques: In Vitro, Cell Culture, Incubation, Labeling

    Collagen triple helix repeat containing 1 ( CTHRC1 ) effects transwell cell migration. (a) Western immunoblotting detection of CTHRC1 in rheumatoid arthritis-fibroblast-like synoviocytes ( RA-FLS ), NIH 3T3, C3H/10 T1/2. RA-FLS express CTHRC1, but murine fibroblasts are negative for CTHRC1. (b) RA-FLS also express cadherin CDH11, alpha smooth muscle actin ( α-SMA ), and α-tubulin. Staining membrane with secondary antibodies alone was negative. (c) CTHRC1 is secreted into conditioned media upon transfection and overexpression in Chinese hamster ovary (CHO) cells. Lanes : (1) positive control 10 ng rhCTHRC1; (2) day 0; (3) day 1; (4) day 3; (5) day 5. (d) RA-FLS were treated directly in transwell chambers with rhCTHRC1 protein, while FBS concentration was varied from 0 %, 1–6 %. Cells that migrated through the membrane were stained with toluidine blue and counted under the microscope. Solid black columns represent cells treated with rhCTHRC1, gray columns are untreated cells. (e) murine C3H/10T1/2 fibroblasts were treated with rhCTHRC1 similarly to RA-FLS. (f) Serum from mice with acute CAIA (5 % serum mixed with 5 % FBS in complete media) showed the strongest promigratory effect. (g) Toluidine blue staining and microscopy of cells migrated through the membrane and spread on its opposite side after the completion of the experiment. Numerous dots are membrane pores of 8 μm in size. Data are representative of three similar but independent experiments. Statistical significance was calculated using Student’s t test: * p

    Journal: Arthritis Research & Therapy

    Article Title: Collagen triple helix repeat containing 1 is a new promigratory marker of arthritic pannus

    doi: 10.1186/s13075-016-1067-1

    Figure Lengend Snippet: Collagen triple helix repeat containing 1 ( CTHRC1 ) effects transwell cell migration. (a) Western immunoblotting detection of CTHRC1 in rheumatoid arthritis-fibroblast-like synoviocytes ( RA-FLS ), NIH 3T3, C3H/10 T1/2. RA-FLS express CTHRC1, but murine fibroblasts are negative for CTHRC1. (b) RA-FLS also express cadherin CDH11, alpha smooth muscle actin ( α-SMA ), and α-tubulin. Staining membrane with secondary antibodies alone was negative. (c) CTHRC1 is secreted into conditioned media upon transfection and overexpression in Chinese hamster ovary (CHO) cells. Lanes : (1) positive control 10 ng rhCTHRC1; (2) day 0; (3) day 1; (4) day 3; (5) day 5. (d) RA-FLS were treated directly in transwell chambers with rhCTHRC1 protein, while FBS concentration was varied from 0 %, 1–6 %. Cells that migrated through the membrane were stained with toluidine blue and counted under the microscope. Solid black columns represent cells treated with rhCTHRC1, gray columns are untreated cells. (e) murine C3H/10T1/2 fibroblasts were treated with rhCTHRC1 similarly to RA-FLS. (f) Serum from mice with acute CAIA (5 % serum mixed with 5 % FBS in complete media) showed the strongest promigratory effect. (g) Toluidine blue staining and microscopy of cells migrated through the membrane and spread on its opposite side after the completion of the experiment. Numerous dots are membrane pores of 8 μm in size. Data are representative of three similar but independent experiments. Statistical significance was calculated using Student’s t test: * p

    Article Snippet: Mouse embryonic fibroblast cell lines C3H/10T1/2 and NIH 3T3, and human skin fibroblast line Hs68 (all lines from ATCC Inc., Manassas, VA, USA) were studied.

    Techniques: Migration, Western Blot, Staining, Transfection, Over Expression, Positive Control, Concentration Assay, Microscopy, Mouse Assay

    Summary of overall effects of the dietary ingredients DHA, β-carotene and resveratrol on lipid accumulation and on gene expression clusters . mRNA levels of enzymes involved in glucose and fat metabolism (Glut4, LPL, FAS, ACC1 and CPT-1β) and of adipocyte differentiation markers (PPARγ, C/EBPα, PPARα, aP2 and adiponectin) were determined in maturing C3H10 T1/2 cells. Depicted are the dose-dependent effects of DHA and β-carotene and the impact of resveratrol on lipid accumulation (after 7 days treatment, top of the table) and on gene expression (after 4 days treatment) relative to rosiglitazone control cells. Data are shown as % of positive control ± SEM (fat accumulation parameters) and fold change ± error (based on SEM, n = 6 - 15) for gene expression levels, respectively. Exemplarily, the fold changes of the genes FAS and PPARγ are shown as illustration. Fat droplet number equates to Spot Count/Object; fat droplet intensity equates to Spot Avg Intensity and fat droplet area equates to Spot Total Area/Object. Student's t-test: treatment versus control (*) p

    Journal: Nutrition & Metabolism

    Article Title: Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes: A novel fluorescent method to quantify fat droplets

    doi: 10.1186/1743-7075-8-30

    Figure Lengend Snippet: Summary of overall effects of the dietary ingredients DHA, β-carotene and resveratrol on lipid accumulation and on gene expression clusters . mRNA levels of enzymes involved in glucose and fat metabolism (Glut4, LPL, FAS, ACC1 and CPT-1β) and of adipocyte differentiation markers (PPARγ, C/EBPα, PPARα, aP2 and adiponectin) were determined in maturing C3H10 T1/2 cells. Depicted are the dose-dependent effects of DHA and β-carotene and the impact of resveratrol on lipid accumulation (after 7 days treatment, top of the table) and on gene expression (after 4 days treatment) relative to rosiglitazone control cells. Data are shown as % of positive control ± SEM (fat accumulation parameters) and fold change ± error (based on SEM, n = 6 - 15) for gene expression levels, respectively. Exemplarily, the fold changes of the genes FAS and PPARγ are shown as illustration. Fat droplet number equates to Spot Count/Object; fat droplet intensity equates to Spot Avg Intensity and fat droplet area equates to Spot Total Area/Object. Student's t-test: treatment versus control (*) p

    Article Snippet: Cell culture Mouse embryonic fibroblast cells C3H10 T1/2 [ ] were from ATCC-LGC (Middlesex, UK) and cultured according to the supplier's protocol.

    Techniques: Expressing, Cycling Probe Technology, Positive Control

    Visualisation of lipid accumulation in differentiated adipocytes . C3H10 T1/2 cells were treated for 7 days with rosiglitazone (0.01, 0.1, 1 and 10 μM) and, after fixation with 60% isopropanol, stained with A) Oil Red O or B) Hoechst 33342 and BODIPY ® 493/503. Images were acquired using A) a Nikon Coolpix 990 camera at 20× magnification or B) the Cellomics ® HCS Reader camera (20×).

    Journal: Nutrition & Metabolism

    Article Title: Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes: A novel fluorescent method to quantify fat droplets

    doi: 10.1186/1743-7075-8-30

    Figure Lengend Snippet: Visualisation of lipid accumulation in differentiated adipocytes . C3H10 T1/2 cells were treated for 7 days with rosiglitazone (0.01, 0.1, 1 and 10 μM) and, after fixation with 60% isopropanol, stained with A) Oil Red O or B) Hoechst 33342 and BODIPY ® 493/503. Images were acquired using A) a Nikon Coolpix 990 camera at 20× magnification or B) the Cellomics ® HCS Reader camera (20×).

    Article Snippet: Cell culture Mouse embryonic fibroblast cells C3H10 T1/2 [ ] were from ATCC-LGC (Middlesex, UK) and cultured according to the supplier's protocol.

    Techniques: Staining

    Effects of DHA, EPA, β-carotene, HT, (all-E)-lycopene, EGCG and resveratrol on lipid formation measured with the HCA assay . C3H10 T1/2 cells were treated for 7 days. Shown are three parameters (Spot Count/Object, Spot Avg Intensity and Spot Total Area/Object) that quantify lipid formation, as compared to rosiglitazone controls set as 100%. Data are shown as mean ± SEM (n = 10 - 20). Student's t-test: treatment versus control (*) p

    Journal: Nutrition & Metabolism

    Article Title: Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes: A novel fluorescent method to quantify fat droplets

    doi: 10.1186/1743-7075-8-30

    Figure Lengend Snippet: Effects of DHA, EPA, β-carotene, HT, (all-E)-lycopene, EGCG and resveratrol on lipid formation measured with the HCA assay . C3H10 T1/2 cells were treated for 7 days. Shown are three parameters (Spot Count/Object, Spot Avg Intensity and Spot Total Area/Object) that quantify lipid formation, as compared to rosiglitazone controls set as 100%. Data are shown as mean ± SEM (n = 10 - 20). Student's t-test: treatment versus control (*) p

    Article Snippet: Cell culture Mouse embryonic fibroblast cells C3H10 T1/2 [ ] were from ATCC-LGC (Middlesex, UK) and cultured according to the supplier's protocol.

    Techniques: High Content Screening

    Effects of varying concentrations of DHA, EPA, β-carotene, (all-E)-lycopene, resveratrol and EGCG on number and intensity of fat droplets . C3H10 T1/2 cells were differentiated in the presence of test substances for 7 days. Spot Count/Object and Spot Avg Intensity were determined, as compared to rosiglitazone control set as 100%. Data are shown as mean ± SEM (n = 10 - 20). Student's t-test: treatment versus control (*) p

    Journal: Nutrition & Metabolism

    Article Title: Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes: A novel fluorescent method to quantify fat droplets

    doi: 10.1186/1743-7075-8-30

    Figure Lengend Snippet: Effects of varying concentrations of DHA, EPA, β-carotene, (all-E)-lycopene, resveratrol and EGCG on number and intensity of fat droplets . C3H10 T1/2 cells were differentiated in the presence of test substances for 7 days. Spot Count/Object and Spot Avg Intensity were determined, as compared to rosiglitazone control set as 100%. Data are shown as mean ± SEM (n = 10 - 20). Student's t-test: treatment versus control (*) p

    Article Snippet: Cell culture Mouse embryonic fibroblast cells C3H10 T1/2 [ ] were from ATCC-LGC (Middlesex, UK) and cultured according to the supplier's protocol.

    Techniques: