Journal: Applied and Environmental Microbiology

Article Title: Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

doi: 10.1128/AEM.00277-17

Figure Lengend Snippet: (A) LTF-MB recovery efficiency was tested using the LTF-MB recovery protocol with 104 CFU · ml−1 of different bacteria. As a control, S. Typhimurium recovery was tested using biotin-coated MBs. The t test was used to calculate the statistical differences of individual recovery efficiencies compared to that of S. Typhimurium DB7155. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P value < 0.0001. (B) The recovery efficiency of S. Typhimurium is not affected by a mixed background flora containing common foodborne pathogens. S. Typhimurium recovery efficiency of LTF-MBs was also tested using S. Typhimurium alone. (1) Salmonella with E. coli K-12 at a ratio of 1:1. (2) Salmonella with E. coli K-12 at a ratio of 1:10. (3) Salmonella with E. coli K-12 at a ratio of 1:100. (4) Salmonella with Bacillus cereus ATCC 14579, Staphylococcus aureus NCTC 8325, Citrobacter freundii N0106, and Cronobacter sakazakii BAA894 at a ratio of 1:1:1:1:1. (5) S. Typhimurium recovery using biotin-passivated MBs as negative control. The differences in the recovery efficiencies of Salmonella alone or under conditions 1 to 4 were not significant (ns). Results are displayed as mean values ± SD.

Article Snippet: E. coli strains BL21(DE3) and Lemo21(DE3) (New England BioLabs [NEB], USA) were used for protein expression. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Species, subspecies, or serovar Phenotype Strain Source a Salmonella serovars and species S . Typhimurium DB7155 1 S . Enteritidis C 2 S. enterica subsp. enterica serovar Hadar WS 2691 1 S. enterica subsp. enterica serovar Heidelberg N2743-08 1 S. enterica subsp. enterica serovar Indiana 2 S. enterica subsp. enterica serovar Infantis 1 S. enterica subsp. enterica serovar Javiana N2427-08 3 S . Montevideo WS2678 1 S. enterica subsp. enterica serovar Newport WS2681 1 S . Newport N2932-08 3 S . Typhimurium LT2 ATCC 14028 4 S . Typhimurium N11-2679 5 S . Typhimurium N11-2063 5 S . Typhimurium N07-1209 5 S . Typhimurium N11-2663 5 S . Typhimurium N11-2388 5 S. enterica subsp. arizonae 56:z4,z23:− N09-0860 3 S. enterica subsp. diarizonae 61:c:z35 N09-2338 3 S. enterica subsp. salamae 30: l,z28:z6 N09-2794 3 S. enterica subsp. houtenae 38:z4,z23:− N09-2589 3 S. bongori 48:z35:− N268-08 3 Non- Salmonella species Bacillus cereus ATCC 14579 1 Bacillus cereus HER 1399 1 Bacillus atrophaeus ( subtilis ) ATCC 9372 1 Citrobacter freundii N 0106 1 Cronobacter sakazakii ATCC BAA-894 1 Cronobacter sakazakii ATCC 29544 1 Enterobacter aerogenes DSM 30053 1 Escherichia coli LPS chemotype K-12 CGSC4401 6 Escherichia vulneris DSM 4564 1 Klebsiella pneumoniae 1319 1 Listeria monocytogenes Serovar 1/2c WSLC 1001 1 Staphylococcus aureus NCTC 8325 1 Staphylococcus aureus ATCC 19685 1 Open in a separate window a Sources of strains: 1, laboratory stock; 2, University of Würzburg, Würzburg, Germany; 3, National Reference Centre for Enteropathogenic Bacteria and Listeria (NENT) (Zurich, Switzerland); 4, Thilo Fuchs (Technical University of Munich, Munich, Germany); 5, Roger Stephan and Herbert Hächler (University of Zurich, Zurich, Switzerland) ( 56 ); 6, Coli Genetic Stock Center (CGSC) (Yale University, New Haven, CT, USA).

Techniques: Negative Control

Journal: Applied and Environmental Microbiology

Article Title: Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

doi: 10.1128/AEM.00277-17

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: E. coli strains BL21(DE3) and Lemo21(DE3) (New England BioLabs [NEB], USA) were used for protein expression. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Species, subspecies, or serovar Phenotype Strain Source a Salmonella serovars and species S . Typhimurium DB7155 1 S . Enteritidis C 2 S. enterica subsp. enterica serovar Hadar WS 2691 1 S. enterica subsp. enterica serovar Heidelberg N2743-08 1 S. enterica subsp. enterica serovar Indiana 2 S. enterica subsp. enterica serovar Infantis 1 S. enterica subsp. enterica serovar Javiana N2427-08 3 S . Montevideo WS2678 1 S. enterica subsp. enterica serovar Newport WS2681 1 S . Newport N2932-08 3 S . Typhimurium LT2 ATCC 14028 4 S . Typhimurium N11-2679 5 S . Typhimurium N11-2063 5 S . Typhimurium N07-1209 5 S . Typhimurium N11-2663 5 S . Typhimurium N11-2388 5 S. enterica subsp. arizonae 56:z4,z23:− N09-0860 3 S. enterica subsp. diarizonae 61:c:z35 N09-2338 3 S. enterica subsp. salamae 30: l,z28:z6 N09-2794 3 S. enterica subsp. houtenae 38:z4,z23:− N09-2589 3 S. bongori 48:z35:− N268-08 3 Non- Salmonella species Bacillus cereus ATCC 14579 1 Bacillus cereus HER 1399 1 Bacillus atrophaeus ( subtilis ) ATCC 9372 1 Citrobacter freundii N 0106 1 Cronobacter sakazakii ATCC BAA-894 1 Cronobacter sakazakii ATCC 29544 1 Enterobacter aerogenes DSM 30053 1 Escherichia coli LPS chemotype K-12 CGSC4401 6 Escherichia vulneris DSM 4564 1 Klebsiella pneumoniae 1319 1 Listeria monocytogenes Serovar 1/2c WSLC 1001 1 Staphylococcus aureus NCTC 8325 1 Staphylococcus aureus ATCC 19685 1 Open in a separate window a Sources of strains: 1, laboratory stock; 2, University of Würzburg, Würzburg, Germany; 3, National Reference Centre for Enteropathogenic Bacteria and Listeria (NENT) (Zurich, Switzerland); 4, Thilo Fuchs (Technical University of Munich, Munich, Germany); 5, Roger Stephan and Herbert Hächler (University of Zurich, Zurich, Switzerland) ( 56 ); 6, Coli Genetic Stock Center (CGSC) (Yale University, New Haven, CT, USA).

Techniques:

Journal:

Article Title: Species Differentiation of a Diverse Suite of Bacillus Spores by Mass Spectrometry-Based Protein Profiling

doi: 10.1128/AEM.70.1.475-482.2004

Figure Lengend Snippet: List of Bacillus species evaluated in this study

Article Snippet: At each rank, the standard deviation of the measurement across the 20 spectra is represented by the error bars. table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Organism and strain B. atrophaeus ATCC 9372 B. licheniformis ATCC 14580 B. licheniformis KL-196 B. megaterium ATCC 14581 B. mojavensis ATCC 51516 B. odysseyi PTA-4399 B. psychrodurans VSE1-06 B. pumilus ATCC 7061 B. subtilis 168 B. subtilis ATCC 6051 B. thuringiensis ATCC 10792 B. atrophaeus ATCC 9372 1 B. licheniformis ATCC 14580 0.09 1 B. licheniformis KL-196 0.03 0.90 1 B. megaterium ATCC 14581 0.14 0.30 0.09 1 B. mojavensis ATCC 51516 0.20 0.23 0.07 0.37 1 B. odysseyi PTA-4399 0.35 0.15 0.05 0.26 0.21 1 B. psychrodurans VSE1-06 0.04 0.45 0.42 0.35 0.05 0.16 1 B. pumilus ATCC 7061 0.14 0.27 0.04 0.45 0.44 0.34 0.07 1 B. subtilis 168 0.09 0.04 0.01 0.11 0.07 0.05 −0.01 0.02 1 B. subtilis ATCC 6051 0.23 0.05 0.01 0.11 0.07 0.29 0.01 0.05 0.88 1 B. thuringiensis ATCC 10792 0.52 0.06 0.03 0.07 0.05 0.33 0.08 0.09 0.02 0.13 1 Open in a separate window Correlation values for MALDI-TOFMS spectra of various Bacillus species To ascertain the robustness of the technique, separate spectra collected and averaged from the same spore culture were examined.

Techniques:

Journal:

Article Title: Characterization of Enterocoliticin, a Phage Tail-Like Bacteriocin, and Its Effect on Pathogenic Yersinia enterocolitica Strains

doi: 10.1128/AEM.67.12.5634-5642.2001

Figure Lengend Snippet: Inhibitory spectrum of enterocoliticin

Article Snippet: 13 3 Escherichia coli RKI K-12, various serogroups 28 0 Salmonella enterica serovar Typhimurium RKI LT1, LT2, LT3 3 0 Salmonella enterica serovar Enteritidis RKI 1 0 Salmonella enterica serovar Derby RKI 1 0 Proteus mirabilis RKI Tetracycline resistant 1 0 Shigella dysenteriae RKI Type 2 1 0 Citrobacter freundii RKI 1 0 Shigella sonnei RKI 1 0 Enterobacter cloacae RKI 1 0 Vibrio harveyi DSM 2332, DSM 6904 2 0 Pseudomonas fluorescens ATCC 6538 1 0 Pseudomonas aeruginosa ATCC 15492, ATCC 27853 2 0 Micrococcus luteus ATCC 4698 1 0 Staphylococcus aureus ATCC 25923, ATCC 6538 2 0 Bacillus subtilis ATCC 9372 1 0 Streptococcus faecium DSM 2146 1 0 Open in a separate window a Abbreviations: RKI, strain collection of the Robert Koch Institute; IP, strains from the Institute Pasteur, Paris, France. b Tests were repeated three times for Yersinia strains by spotting a twofold serial dilution of enterocoliticin and incubating at 37°C. (The highest enterocoliticin activity was 8.8 × 10 −5 AU ml −1 , determined on the reference strain Y. enterocolitica 13169).

Techniques: