al1852 δ lpxd  (ATCC)


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    Structured Review

    ATCC al1852 δ lpxd
    Bacterial strains used in this study
    Al1852 δ Lpxd, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/al1852 δ lpxd/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    al1852 δ lpxd - by Bioz Stars, 2023-06
    85/100 stars

    Images

    1) Product Images from "Biological Cost of Different Mechanisms of Colistin Resistance and Their Impact on Virulence in Acinetobacter baumannii"

    Article Title: Biological Cost of Different Mechanisms of Colistin Resistance and Their Impact on Virulence in Acinetobacter baumannii

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.01597-13

    Bacterial strains used in this study
    Figure Legend Snippet: Bacterial strains used in this study

    Techniques Used: Isolation, Binding Assay

    A. baumannii doubling times and growth rates measured in the exponential phase of growth over the first 300 min a
    Figure Legend Snippet: A. baumannii doubling times and growth rates measured in the exponential phase of growth over the first 300 min a

    Techniques Used:

    Abilities of different A. baumannii strains to cause cell death of A549 alveolar cells. (A) Fluorescence microscopy images of human alveolar A549 cells infected with each of the A. baumannii strains and stained with the LIVE/DEAD Cellstain double-staining kit. Healthy cells with intact membranes are stained green, and dead cells with permeabilized membranes are stained red. A549 cells were incubated with the A. baumannii strains ATCC 19606 WT, AL1851 ΔlpxA, Al1852 ΔlpxD, AL1842 ΔlpxC, and ATCC 19606 pmrB for 20 h or left uninfected. (B) Quantification of A549 cell death caused by A. baumannii ATCC 19606 WT and AL1851 ΔlpxA, AL1852 ΔlpxD, and AL1842 ΔlpxC mutants and the pmrB mutant. The results of 6 independent experiments are shown as means and SD. *, P < 0.01 between the parental strain and each of the designated mutants; **, not statistically different.
    Figure Legend Snippet: Abilities of different A. baumannii strains to cause cell death of A549 alveolar cells. (A) Fluorescence microscopy images of human alveolar A549 cells infected with each of the A. baumannii strains and stained with the LIVE/DEAD Cellstain double-staining kit. Healthy cells with intact membranes are stained green, and dead cells with permeabilized membranes are stained red. A549 cells were incubated with the A. baumannii strains ATCC 19606 WT, AL1851 ΔlpxA, Al1852 ΔlpxD, AL1842 ΔlpxC, and ATCC 19606 pmrB for 20 h or left uninfected. (B) Quantification of A549 cell death caused by A. baumannii ATCC 19606 WT and AL1851 ΔlpxA, AL1852 ΔlpxD, and AL1842 ΔlpxC mutants and the pmrB mutant. The results of 6 independent experiments are shown as means and SD. *, P < 0.01 between the parental strain and each of the designated mutants; **, not statistically different.

    Techniques Used: Fluorescence, Microscopy, Infection, Staining, Double Staining, Incubation, Mutagenesis

    C. elegans fertility assay
    Figure Legend Snippet: C. elegans fertility assay

    Techniques Used:

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    • al1852 δ lpxd  (ATCC)
    85
    ATCC al1852 δ lpxd
    Bacterial strains used in this study
    Al1852 δ Lpxd, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/al1852 δ lpxd/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    al1852 δ lpxd - by Bioz Stars, 2023-06
    85/100 stars
    		  Buy from Supplier

    Image Search Results


    Bacterial strains used in this study

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Biological Cost of Different Mechanisms of Colistin Resistance and Their Impact on Virulence in Acinetobacter baumannii

    doi: 10.1128/AAC.01597-13

    Figure Lengend Snippet: Bacterial strains used in this study

    Article Snippet: The error bars represent the standard deviation. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Quantification (per day) on day a : Total no. of progeny a Difference from control b (%) 1 2 3 4 A. baumannii ATCC 19606 45 99.3 47.7 2.33 194.2 ± 6.9 79.5 AL1851 Δ lpxA 70.2 158.7 87.3 0.7 316.9 ± 12 129.7 AL1852 Δ lpxD 73.7 202.7 61.3 0.7 338.3 ± 26 138.4 AL1842 Δ lpxC 62.5 147.2 72.3 2.3 284.3 ± 50 116.3 ATCC 19606 pmrB 46.33 92 37.2 9.7 180.2 ± 22 73.7 ABRIM 37.3 83.7 76.5 2.8 200.3 ± 13 81.9 ABRIM pmrB 38.8 75 75.3 13.5 202.7 ± 30.3 82.9 E. coli OP50 38.6 122.4 73.2 10.2 244.4 ± 28 100 Open in a separate window a Mean quantification and total no. of progeny by worm. b Percentage of progeny compared to E. coli OP50.

    Techniques: Isolation, Binding Assay

    A. baumannii doubling times and growth rates measured in the exponential phase of growth over the first 300 min a

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Biological Cost of Different Mechanisms of Colistin Resistance and Their Impact on Virulence in Acinetobacter baumannii

    doi: 10.1128/AAC.01597-13

    Figure Lengend Snippet: A. baumannii doubling times and growth rates measured in the exponential phase of growth over the first 300 min a

    Article Snippet: The error bars represent the standard deviation. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Quantification (per day) on day a : Total no. of progeny a Difference from control b (%) 1 2 3 4 A. baumannii ATCC 19606 45 99.3 47.7 2.33 194.2 ± 6.9 79.5 AL1851 Δ lpxA 70.2 158.7 87.3 0.7 316.9 ± 12 129.7 AL1852 Δ lpxD 73.7 202.7 61.3 0.7 338.3 ± 26 138.4 AL1842 Δ lpxC 62.5 147.2 72.3 2.3 284.3 ± 50 116.3 ATCC 19606 pmrB 46.33 92 37.2 9.7 180.2 ± 22 73.7 ABRIM 37.3 83.7 76.5 2.8 200.3 ± 13 81.9 ABRIM pmrB 38.8 75 75.3 13.5 202.7 ± 30.3 82.9 E. coli OP50 38.6 122.4 73.2 10.2 244.4 ± 28 100 Open in a separate window a Mean quantification and total no. of progeny by worm. b Percentage of progeny compared to E. coli OP50.

    Techniques:

    Abilities of different A. baumannii strains to cause cell death of A549 alveolar cells. (A) Fluorescence microscopy images of human alveolar A549 cells infected with each of the A. baumannii strains and stained with the LIVE/DEAD Cellstain double-staining kit. Healthy cells with intact membranes are stained green, and dead cells with permeabilized membranes are stained red. A549 cells were incubated with the A. baumannii strains ATCC 19606 WT, AL1851 ΔlpxA, Al1852 ΔlpxD, AL1842 ΔlpxC, and ATCC 19606 pmrB for 20 h or left uninfected. (B) Quantification of A549 cell death caused by A. baumannii ATCC 19606 WT and AL1851 ΔlpxA, AL1852 ΔlpxD, and AL1842 ΔlpxC mutants and the pmrB mutant. The results of 6 independent experiments are shown as means and SD. *, P < 0.01 between the parental strain and each of the designated mutants; **, not statistically different.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Biological Cost of Different Mechanisms of Colistin Resistance and Their Impact on Virulence in Acinetobacter baumannii

    doi: 10.1128/AAC.01597-13

    Figure Lengend Snippet: Abilities of different A. baumannii strains to cause cell death of A549 alveolar cells. (A) Fluorescence microscopy images of human alveolar A549 cells infected with each of the A. baumannii strains and stained with the LIVE/DEAD Cellstain double-staining kit. Healthy cells with intact membranes are stained green, and dead cells with permeabilized membranes are stained red. A549 cells were incubated with the A. baumannii strains ATCC 19606 WT, AL1851 ΔlpxA, Al1852 ΔlpxD, AL1842 ΔlpxC, and ATCC 19606 pmrB for 20 h or left uninfected. (B) Quantification of A549 cell death caused by A. baumannii ATCC 19606 WT and AL1851 ΔlpxA, AL1852 ΔlpxD, and AL1842 ΔlpxC mutants and the pmrB mutant. The results of 6 independent experiments are shown as means and SD. *, P < 0.01 between the parental strain and each of the designated mutants; **, not statistically different.

    Article Snippet: The error bars represent the standard deviation. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Quantification (per day) on day a : Total no. of progeny a Difference from control b (%) 1 2 3 4 A. baumannii ATCC 19606 45 99.3 47.7 2.33 194.2 ± 6.9 79.5 AL1851 Δ lpxA 70.2 158.7 87.3 0.7 316.9 ± 12 129.7 AL1852 Δ lpxD 73.7 202.7 61.3 0.7 338.3 ± 26 138.4 AL1842 Δ lpxC 62.5 147.2 72.3 2.3 284.3 ± 50 116.3 ATCC 19606 pmrB 46.33 92 37.2 9.7 180.2 ± 22 73.7 ABRIM 37.3 83.7 76.5 2.8 200.3 ± 13 81.9 ABRIM pmrB 38.8 75 75.3 13.5 202.7 ± 30.3 82.9 E. coli OP50 38.6 122.4 73.2 10.2 244.4 ± 28 100 Open in a separate window a Mean quantification and total no. of progeny by worm. b Percentage of progeny compared to E. coli OP50.

    Techniques: Fluorescence, Microscopy, Infection, Staining, Double Staining, Incubation, Mutagenesis

    C. elegans fertility assay

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Biological Cost of Different Mechanisms of Colistin Resistance and Their Impact on Virulence in Acinetobacter baumannii

    doi: 10.1128/AAC.01597-13

    Figure Lengend Snippet: C. elegans fertility assay

    Article Snippet: The error bars represent the standard deviation. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain Quantification (per day) on day a : Total no. of progeny a Difference from control b (%) 1 2 3 4 A. baumannii ATCC 19606 45 99.3 47.7 2.33 194.2 ± 6.9 79.5 AL1851 Δ lpxA 70.2 158.7 87.3 0.7 316.9 ± 12 129.7 AL1852 Δ lpxD 73.7 202.7 61.3 0.7 338.3 ± 26 138.4 AL1842 Δ lpxC 62.5 147.2 72.3 2.3 284.3 ± 50 116.3 ATCC 19606 pmrB 46.33 92 37.2 9.7 180.2 ± 22 73.7 ABRIM 37.3 83.7 76.5 2.8 200.3 ± 13 81.9 ABRIM pmrB 38.8 75 75.3 13.5 202.7 ± 30.3 82.9 E. coli OP50 38.6 122.4 73.2 10.2 244.4 ± 28 100 Open in a separate window a Mean quantification and total no. of progeny by worm. b Percentage of progeny compared to E. coli OP50.

    Techniques: