Structured Review

Millipore mouse alkbh5
(A) Comparison of the expression levels of <t>ALKBH5</t> in primary AML patients bearing various chromosomal translocations with those in BM hematopoietic stem cells (HSCs) collected from healthy donors (NC) based on the GSE13159 and GSE42519 datasets. The expression values (detected by Affymetrix exon arrays) were log2-transformed.
Mouse Alkbh5, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse alkbh5/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse alkbh5 - by Bioz Stars, 2024-07
86/100 stars

Images

1) Product Images from "M 6 A demethylase ALKBH5 selectively promotes tumorigenesis and cancer stem cell self-renewal in acute myeloid leukemia"

Article Title: M 6 A demethylase ALKBH5 selectively promotes tumorigenesis and cancer stem cell self-renewal in acute myeloid leukemia

Journal: Cell stem cell

doi: 10.1016/j.stem.2020.04.009

(A) Comparison of the expression levels of ALKBH5 in primary AML patients bearing various chromosomal translocations with those in BM hematopoietic stem cells (HSCs) collected from healthy donors (NC) based on the GSE13159 and GSE42519 datasets. The expression values (detected by Affymetrix exon arrays) were log2-transformed.
Figure Legend Snippet: (A) Comparison of the expression levels of ALKBH5 in primary AML patients bearing various chromosomal translocations with those in BM hematopoietic stem cells (HSCs) collected from healthy donors (NC) based on the GSE13159 and GSE42519 datasets. The expression values (detected by Affymetrix exon arrays) were log2-transformed.

Techniques Used: Expressing, Transformation Assay

(A-C) BM lin- cells from Alkbh5 WT or homozygous KO mice were transduced with MA9-YFP retrovirus and then transplanted into primary recipient mice. Five mice from each transplant group were euthanized at the same time (day 42 post-BMT) for LSC/LIC analysis. (A) Representative flow cytometry analysis of LSCs/LICs in BM. (B) Percentage of leukemic GMPs (LGMPs) in YFP+ cells. (C) Percentage of apoptotic LGMPs.
Figure Legend Snippet: (A-C) BM lin- cells from Alkbh5 WT or homozygous KO mice were transduced with MA9-YFP retrovirus and then transplanted into primary recipient mice. Five mice from each transplant group were euthanized at the same time (day 42 post-BMT) for LSC/LIC analysis. (A) Representative flow cytometry analysis of LSCs/LICs in BM. (B) Percentage of leukemic GMPs (LGMPs) in YFP+ cells. (C) Percentage of apoptotic LGMPs.

Techniques Used: Transduction, Flow Cytometry

(A) Scheme of the design and procedures of generation of Alkbh5 knockout (KO) mice using the CRISPR-Cas9 technology.
Figure Legend Snippet: (A) Scheme of the design and procedures of generation of Alkbh5 knockout (KO) mice using the CRISPR-Cas9 technology.

Techniques Used: Knock-Out, CRISPR

(A-C) RNA-seq analysis of gene expression profiles in ALKBH5 knockdown AML cells and control AML cells. (A) Venn diagram shows numbers of genes with significant changes in expression (RPKM>1, fold change>1.5) upon ALKBH5 knockdown. (B) GSEA of up- and down-regulated genes. (C) Violin plots showing the relative abundance of genes involved in the indicated pathways in ALKBH5 knockdown or control NOMO1 cells.
Figure Legend Snippet: (A-C) RNA-seq analysis of gene expression profiles in ALKBH5 knockdown AML cells and control AML cells. (A) Venn diagram shows numbers of genes with significant changes in expression (RPKM>1, fold change>1.5) upon ALKBH5 knockdown. (B) GSEA of up- and down-regulated genes. (C) Violin plots showing the relative abundance of genes involved in the indicated pathways in ALKBH5 knockdown or control NOMO1 cells.

Techniques Used: RNA Sequencing Assay, Expressing

(A) Primary mouse leukemic BM cells were transduced with lentiviruses expressing control shRNA (shNS) and two independent shRNAs targeting Alkbh5 (shA5-#a and shA5-#b) and serially replated. Colony forming cell counts at each round of plating were shown (n=3).
Figure Legend Snippet: (A) Primary mouse leukemic BM cells were transduced with lentiviruses expressing control shRNA (shNS) and two independent shRNAs targeting Alkbh5 (shA5-#a and shA5-#b) and serially replated. Colony forming cell counts at each round of plating were shown (n=3).

Techniques Used: Transduction, Expressing, shRNA

KEY RESOURCES TABLE
Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Recombinant, Magnetic Beads, Proliferation Assay, Purification, Real-time Polymerase Chain Reaction, Software


Structured Review

Millipore mouse alkbh5
(A) Comparison of the expression levels of <t>ALKBH5</t> in primary AML patients bearing various chromosomal translocations with those in BM hematopoietic stem cells (HSCs) collected from healthy donors (NC) based on the GSE13159 and GSE42519 datasets. The expression values (detected by Affymetrix exon arrays) were log2-transformed.
Mouse Alkbh5, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse alkbh5/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse alkbh5 - by Bioz Stars, 2024-07
86/100 stars

Images

1) Product Images from "M 6 A demethylase ALKBH5 selectively promotes tumorigenesis and cancer stem cell self-renewal in acute myeloid leukemia"

Article Title: M 6 A demethylase ALKBH5 selectively promotes tumorigenesis and cancer stem cell self-renewal in acute myeloid leukemia

Journal: Cell stem cell

doi: 10.1016/j.stem.2020.04.009

(A) Comparison of the expression levels of ALKBH5 in primary AML patients bearing various chromosomal translocations with those in BM hematopoietic stem cells (HSCs) collected from healthy donors (NC) based on the GSE13159 and GSE42519 datasets. The expression values (detected by Affymetrix exon arrays) were log2-transformed.
Figure Legend Snippet: (A) Comparison of the expression levels of ALKBH5 in primary AML patients bearing various chromosomal translocations with those in BM hematopoietic stem cells (HSCs) collected from healthy donors (NC) based on the GSE13159 and GSE42519 datasets. The expression values (detected by Affymetrix exon arrays) were log2-transformed.

Techniques Used: Expressing, Transformation Assay

(A-C) BM lin- cells from Alkbh5 WT or homozygous KO mice were transduced with MA9-YFP retrovirus and then transplanted into primary recipient mice. Five mice from each transplant group were euthanized at the same time (day 42 post-BMT) for LSC/LIC analysis. (A) Representative flow cytometry analysis of LSCs/LICs in BM. (B) Percentage of leukemic GMPs (LGMPs) in YFP+ cells. (C) Percentage of apoptotic LGMPs.
Figure Legend Snippet: (A-C) BM lin- cells from Alkbh5 WT or homozygous KO mice were transduced with MA9-YFP retrovirus and then transplanted into primary recipient mice. Five mice from each transplant group were euthanized at the same time (day 42 post-BMT) for LSC/LIC analysis. (A) Representative flow cytometry analysis of LSCs/LICs in BM. (B) Percentage of leukemic GMPs (LGMPs) in YFP+ cells. (C) Percentage of apoptotic LGMPs.

Techniques Used: Transduction, Flow Cytometry

(A) Scheme of the design and procedures of generation of Alkbh5 knockout (KO) mice using the CRISPR-Cas9 technology.
Figure Legend Snippet: (A) Scheme of the design and procedures of generation of Alkbh5 knockout (KO) mice using the CRISPR-Cas9 technology.

Techniques Used: Knock-Out, CRISPR

(A-C) RNA-seq analysis of gene expression profiles in ALKBH5 knockdown AML cells and control AML cells. (A) Venn diagram shows numbers of genes with significant changes in expression (RPKM>1, fold change>1.5) upon ALKBH5 knockdown. (B) GSEA of up- and down-regulated genes. (C) Violin plots showing the relative abundance of genes involved in the indicated pathways in ALKBH5 knockdown or control NOMO1 cells.
Figure Legend Snippet: (A-C) RNA-seq analysis of gene expression profiles in ALKBH5 knockdown AML cells and control AML cells. (A) Venn diagram shows numbers of genes with significant changes in expression (RPKM>1, fold change>1.5) upon ALKBH5 knockdown. (B) GSEA of up- and down-regulated genes. (C) Violin plots showing the relative abundance of genes involved in the indicated pathways in ALKBH5 knockdown or control NOMO1 cells.

Techniques Used: RNA Sequencing Assay, Expressing

(A) Primary mouse leukemic BM cells were transduced with lentiviruses expressing control shRNA (shNS) and two independent shRNAs targeting Alkbh5 (shA5-#a and shA5-#b) and serially replated. Colony forming cell counts at each round of plating were shown (n=3).
Figure Legend Snippet: (A) Primary mouse leukemic BM cells were transduced with lentiviruses expressing control shRNA (shNS) and two independent shRNAs targeting Alkbh5 (shA5-#a and shA5-#b) and serially replated. Colony forming cell counts at each round of plating were shown (n=3).

Techniques Used: Transduction, Expressing, shRNA

KEY RESOURCES TABLE
Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Recombinant, Magnetic Beads, Proliferation Assay, Purification, Real-time Polymerase Chain Reaction, Software


Structured Review

Millipore mouse alkbh5
(A) Comparison of the expression levels of <t>ALKBH5</t> in primary AML patients bearing various chromosomal translocations with those in BM hematopoietic stem cells (HSCs) collected from healthy donors (NC) based on the GSE13159 and GSE42519 datasets. The expression values (detected by Affymetrix exon arrays) were log2-transformed.
Mouse Alkbh5, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse alkbh5/product/Millipore
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse alkbh5 - by Bioz Stars, 2024-07
86/100 stars

Images

1) Product Images from "M 6 A demethylase ALKBH5 selectively promotes tumorigenesis and cancer stem cell self-renewal in acute myeloid leukemia"

Article Title: M 6 A demethylase ALKBH5 selectively promotes tumorigenesis and cancer stem cell self-renewal in acute myeloid leukemia

Journal: Cell stem cell

doi: 10.1016/j.stem.2020.04.009

(A) Comparison of the expression levels of ALKBH5 in primary AML patients bearing various chromosomal translocations with those in BM hematopoietic stem cells (HSCs) collected from healthy donors (NC) based on the GSE13159 and GSE42519 datasets. The expression values (detected by Affymetrix exon arrays) were log2-transformed.
Figure Legend Snippet: (A) Comparison of the expression levels of ALKBH5 in primary AML patients bearing various chromosomal translocations with those in BM hematopoietic stem cells (HSCs) collected from healthy donors (NC) based on the GSE13159 and GSE42519 datasets. The expression values (detected by Affymetrix exon arrays) were log2-transformed.

Techniques Used: Expressing, Transformation Assay

(A-C) BM lin- cells from Alkbh5 WT or homozygous KO mice were transduced with MA9-YFP retrovirus and then transplanted into primary recipient mice. Five mice from each transplant group were euthanized at the same time (day 42 post-BMT) for LSC/LIC analysis. (A) Representative flow cytometry analysis of LSCs/LICs in BM. (B) Percentage of leukemic GMPs (LGMPs) in YFP+ cells. (C) Percentage of apoptotic LGMPs.
Figure Legend Snippet: (A-C) BM lin- cells from Alkbh5 WT or homozygous KO mice were transduced with MA9-YFP retrovirus and then transplanted into primary recipient mice. Five mice from each transplant group were euthanized at the same time (day 42 post-BMT) for LSC/LIC analysis. (A) Representative flow cytometry analysis of LSCs/LICs in BM. (B) Percentage of leukemic GMPs (LGMPs) in YFP+ cells. (C) Percentage of apoptotic LGMPs.

Techniques Used: Transduction, Flow Cytometry

(A) Scheme of the design and procedures of generation of Alkbh5 knockout (KO) mice using the CRISPR-Cas9 technology.
Figure Legend Snippet: (A) Scheme of the design and procedures of generation of Alkbh5 knockout (KO) mice using the CRISPR-Cas9 technology.

Techniques Used: Knock-Out, CRISPR

(A-C) RNA-seq analysis of gene expression profiles in ALKBH5 knockdown AML cells and control AML cells. (A) Venn diagram shows numbers of genes with significant changes in expression (RPKM>1, fold change>1.5) upon ALKBH5 knockdown. (B) GSEA of up- and down-regulated genes. (C) Violin plots showing the relative abundance of genes involved in the indicated pathways in ALKBH5 knockdown or control NOMO1 cells.
Figure Legend Snippet: (A-C) RNA-seq analysis of gene expression profiles in ALKBH5 knockdown AML cells and control AML cells. (A) Venn diagram shows numbers of genes with significant changes in expression (RPKM>1, fold change>1.5) upon ALKBH5 knockdown. (B) GSEA of up- and down-regulated genes. (C) Violin plots showing the relative abundance of genes involved in the indicated pathways in ALKBH5 knockdown or control NOMO1 cells.

Techniques Used: RNA Sequencing Assay, Expressing

(A) Primary mouse leukemic BM cells were transduced with lentiviruses expressing control shRNA (shNS) and two independent shRNAs targeting Alkbh5 (shA5-#a and shA5-#b) and serially replated. Colony forming cell counts at each round of plating were shown (n=3).
Figure Legend Snippet: (A) Primary mouse leukemic BM cells were transduced with lentiviruses expressing control shRNA (shNS) and two independent shRNAs targeting Alkbh5 (shA5-#a and shA5-#b) and serially replated. Colony forming cell counts at each round of plating were shown (n=3).

Techniques Used: Transduction, Expressing, shRNA

KEY RESOURCES TABLE
Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Recombinant, Magnetic Beads, Proliferation Assay, Purification, Real-time Polymerase Chain Reaction, Software