16s rrna gene  (ATCC)


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    ATCC 16s rrna gene
    Classification of isolates cultured in this study.
    16s Rrna Gene, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria"

    Article Title: Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria

    Journal: Gut Microbes

    doi: 10.1080/19490976.2018.1491265

    Classification of isolates cultured in this study.
    Figure Legend Snippet: Classification of isolates cultured in this study.

    Techniques Used: Cell Culture, Sequencing

    16s rrna gene  (ATCC)


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    ATCC 16s rrna gene
    Classification of isolates cultured in this study.
    16s Rrna Gene, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria"

    Article Title: Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria

    Journal: Gut Microbes

    doi: 10.1080/19490976.2018.1491265

    Classification of isolates cultured in this study.
    Figure Legend Snippet: Classification of isolates cultured in this study.

    Techniques Used: Cell Culture, Sequencing

    butyrivibrio crossotus atcc 29175 t  (ATCC)


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    ATCC butyrivibrio crossotus atcc 29175 t
    Classification of isolates cultured in this study.
    Butyrivibrio Crossotus Atcc 29175 T, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria"

    Article Title: Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria

    Journal: Gut Microbes

    doi: 10.1080/19490976.2018.1491265

    Classification of isolates cultured in this study.
    Figure Legend Snippet: Classification of isolates cultured in this study.

    Techniques Used: Cell Culture, Sequencing

    citric acid biosynthesis  (ATCC)


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    ATCC citric acid biosynthesis
    Citric Acid Biosynthesis, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    kt750851 45 96 em011 1411 pantoea eucrina lmg 2781 t  (ATCC)


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    ATCC kt750851 45 96 em011 1411 pantoea eucrina lmg 2781 t
    Kt750851 45 96 Em011 1411 Pantoea Eucrina Lmg 2781 T, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92/100 stars

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    mrsa st398 strains jcsc6945  (ATCC)


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    ATCC mrsa st398 strains jcsc6945
    Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA <t>ST398</t> plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.
    Mrsa St398 Strains Jcsc6945, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrsa st398 strains jcsc6945/product/ATCC
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    1) Product Images from "Novel erm (T)-Carrying Multiresistance Plasmids from Porcine and Human Isolates of Methicillin-Resistant Staphylococcus aureus ST398 That Also Harbor Cadmium and Copper Resistance Determinants"

    Article Title: Novel erm (T)-Carrying Multiresistance Plasmids from Porcine and Human Isolates of Methicillin-Resistant Staphylococcus aureus ST398 That Also Harbor Cadmium and Copper Resistance Determinants

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00171-13

    Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA ST398 plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.
    Figure Legend Snippet: Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA ST398 plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.

    Techniques Used: Sequencing, Plasmid Preparation

    mrsa st398 strains jcsc6945  (ATCC)


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    ATCC mrsa st398 strains jcsc6945
    Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA <t>ST398</t> plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.
    Mrsa St398 Strains Jcsc6945, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Novel erm (T)-Carrying Multiresistance Plasmids from Porcine and Human Isolates of Methicillin-Resistant Staphylococcus aureus ST398 That Also Harbor Cadmium and Copper Resistance Determinants"

    Article Title: Novel erm (T)-Carrying Multiresistance Plasmids from Porcine and Human Isolates of Methicillin-Resistant Staphylococcus aureus ST398 That Also Harbor Cadmium and Copper Resistance Determinants

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00171-13

    Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA ST398 plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.
    Figure Legend Snippet: Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA ST398 plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.

    Techniques Used: Sequencing, Plasmid Preparation

    mrsa st398 strains jcsc6945  (ATCC)


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    ATCC mrsa st398 strains jcsc6945
    Mrsa St398 Strains Jcsc6945, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rpob sequence similarity  (ATCC)


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    ATCC rpob sequence similarity
    Rpob Sequence Similarity, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gtc 2738t  (ATCC)


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    ATCC gtc 2738t
    Gtc 2738t, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    silvimonas terrae kctc 12358t  (ATCC)


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    ATCC silvimonas terrae kctc 12358t
    Silvimonas Terrae Kctc 12358t, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Classification of isolates cultured in this study.
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    Classification of isolates cultured in this study.
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    ATCC kt750851 45 96 em011 1411 pantoea eucrina lmg 2781 t
    Classification of isolates cultured in this study.
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    ATCC mrsa st398 strains jcsc6945
    Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA <t>ST398</t> plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.
    Mrsa St398 Strains Jcsc6945, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA <t>ST398</t> plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.
    Rpob Sequence Similarity, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA <t>ST398</t> plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.
    Gtc 2738t, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC silvimonas terrae kctc 12358t
    Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA <t>ST398</t> plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.
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    Classification of isolates cultured in this study.

    Journal: Gut Microbes

    Article Title: Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria

    doi: 10.1080/19490976.2018.1491265

    Figure Lengend Snippet: Classification of isolates cultured in this study.

    Article Snippet: For instance, the GAM79 (OTU117) strain showed the highest nucleotide identity of 16S rRNA gene at 91.9% to Butyrivibrio crossotus ATCC 29175 T , and the other tested genes also showed lower homology, below 80%, indicating that this strain could be potentially assigned as a novel genus candidate rather than a species.

    Techniques: Cell Culture, Sequencing

    Classification of isolates cultured in this study.

    Journal: Gut Microbes

    Article Title: Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria

    doi: 10.1080/19490976.2018.1491265

    Figure Lengend Snippet: Classification of isolates cultured in this study.

    Article Snippet: For instance, the GAM79 (OTU117) strain showed the highest nucleotide identity of 16S rRNA gene at 91.9% to Butyrivibrio crossotus ATCC 29175 T , and the other tested genes also showed lower homology, below 80%, indicating that this strain could be potentially assigned as a novel genus candidate rather than a species.

    Techniques: Cell Culture, Sequencing

    Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA ST398 plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Novel erm (T)-Carrying Multiresistance Plasmids from Porcine and Human Isolates of Methicillin-Resistant Staphylococcus aureus ST398 That Also Harbor Cadmium and Copper Resistance Determinants

    doi: 10.1128/AAC.00171-13

    Figure Lengend Snippet: Sequence alignment of the homologous regions (56 to 59 bp) located upstream (A) and downstream (B) of the erm(T) gene in the three novel MRSA ST398 plasmids (pUR1902, pUR2940, and pUR2941), plasmid pGB2002 (Streptococcus agalactiae), pGB2001 (Streptococcus agalactiae), pGA2000 (Streptococcus pyogenes), p5580 (Streptococcus dysgalactiae), pRW35 (Streptococcus pyogenes), and a possible related precursor, pKKS825 (MRSA ST398) potentially used for the integration of an erm(T)-containing segment into the hypothetical pUR2940 erm(T)-free precursor. Displayed are nucleotides (nt) at the following up- and downstream positions of the erm(T) gene, respectively: nt 339 to 396 and 142 to 199 in pUR2940 (accession number HF583292); nt 524 to 581 and 142 to 197 in pRW35 (EU192194); nt 394 to 451 and 142 to 197 in pGB2002 (JF308629); nt 393 to 450 and 142 to 197 in pGB2001 (JF308630), pGA2000 (JF308631), and p5580 (HE862394); nt 1282 to 1340 downstream of the dfrK gene in pKKS825 (FN377602). Nucleotides in faint gray are those identical in all sequences, nucleotides in black show the identical bases specific for the upstream segments, and those in dark grey show those identical in all downstream regions.

    Article Snippet: The gene cluster copA-mco detected in the three plasmids showed the highest percentage of identity to that identified in the chromosomal DNA of Staphylococcus epidermidis ATCC 12228 (92.8%), Staphylococcus haemolyticus JCSC1435 (92.6%) and the novel SCC mec X and SCC mec IX elements of MRSA ST398 strains JCSC6945 (91.9%) and JCSC6943 (91.3%), respectively.

    Techniques: Sequencing, Plasmid Preparation