Journal: Canadian Journal of Veterinary Research
Article Title: Blocking the expression of syntaxin 4 interferes with initial phagocytosis of Brucella melitensis in macrophages
Figure Lengend Snippet: Expression of STX4 protein extracts from THP-1-V 3 cells 15, 30, 45, and 60 min after infection by (A) S. Enteritidis (SE) or (B) B. melitensis (BM), analyzed by Western blotting, and the corresponding values for uninfected cells. Panel C shows the values
Article Snippet: Cells from the human monocyte THP-1 cell line (ATCC 9184) were incubated at 37°C in 5% CO2 and maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (Life Technologies, Carlsbad, California, USA), 2 mM glutamine, 1 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol, 0.45% glucose, 25 mM HEPES buffer, and a mixture of 100 IU of penicillin and 100 μg of streptomycin per milliliter (Gibco-Invitrogen, Carlsbad, California, USA).
Techniques: Expressing, Infection, Western Blot