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cytochrome c elisa kit  (R&D Systems)
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Release of <t>cytochrome</t> <t>c</t> in HL60 and HL-60R cells treated with MK-4 and MKH derivatives. HL60 and HL-60R cells treated with the test drugs at 10 µM for 48 h ( A ) or 72 h ( B ). The cytochrome c level in the mitochondrial or cytosolic fractions was determined using <t>ELISA.</t> Data are shown as mean ± SD from at least 3 replicates. * p < 0.05, ** p < 0.01 vs. vehicle by Dunnett’s test.
Cytochrome C Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cytochrome c elisa kit/product/R&D Systems
Average 91 stars, based on 1 article reviews
cytochrome c elisa kit - by Bioz Stars, 2025-06
91/100 stars
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cytochrome c elisa kit  (Novus Biologicals)
91
Novus Biologicals cytochrome c elisa kit
( A ) Caspase 9 release from NCE control, LST54 and LST421 cells as measured through Caspase-Glo 9 assay kit over 0, 7.5 and 15 µM cisplatin and the positive control etoposide (200 µM). ( B ) <t>ELISA</t> detection of <t>cytochrome</t> <t>c</t> from both cytoplasmic and mitochondrial fractions in LST421, LST54 and NCE cells after cisplatin exposure (7.5 µM). ( C ) Comparison of the ratio of mitochondrial:cytoplasmic cytochrome c after exposure to cisplatin (7.5 µM). ( D ) Cytochrome c release from mitochondria using Mitotracker green and FITC 568 secondary labelled cytochrome c . ( E ) Mitochondrial depolarisation measured using JC-1 fluorophore in the NCE control and LST421 after exposure to FCCP (20 nM) and cisplatin (7.5 µM). Unpaired t -test used to determine statistical significance. * indicates a p < 0.05. ns = non-significant.
Cytochrome C Elisa Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cytochrome c elisa kit/product/Novus Biologicals
Average 91 stars, based on 1 article reviews
cytochrome c elisa kit - by Bioz Stars, 2025-06
91/100 stars
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220797 plv h1 shsrgap2a hsynapsin egfp wpre  (Addgene inc)
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Addgene inc 220797 plv h1 shsrgap2a hsynapsin egfp wpre
( A ) Caspase 9 release from NCE control, LST54 and LST421 cells as measured through Caspase-Glo 9 assay kit over 0, 7.5 and 15 µM cisplatin and the positive control etoposide (200 µM). ( B ) <t>ELISA</t> detection of <t>cytochrome</t> <t>c</t> from both cytoplasmic and mitochondrial fractions in LST421, LST54 and NCE cells after cisplatin exposure (7.5 µM). ( C ) Comparison of the ratio of mitochondrial:cytoplasmic cytochrome c after exposure to cisplatin (7.5 µM). ( D ) Cytochrome c release from mitochondria using Mitotracker green and FITC 568 secondary labelled cytochrome c . ( E ) Mitochondrial depolarisation measured using JC-1 fluorophore in the NCE control and LST421 after exposure to FCCP (20 nM) and cisplatin (7.5 µM). Unpaired t -test used to determine statistical significance. * indicates a p < 0.05. ns = non-significant.
220797 Plv H1 Shsrgap2a Hsynapsin Egfp Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/220797 plv h1 shsrgap2a hsynapsin egfp wpre/product/Addgene inc
Average 93 stars, based on 1 article reviews
220797 plv h1 shsrgap2a hsynapsin egfp wpre - by Bioz Stars, 2025-06
93/100 stars
  Buy from Supplier

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Release of cytochrome c in HL60 and HL-60R cells treated with MK-4 and MKH derivatives. HL60 and HL-60R cells treated with the test drugs at 10 µM for 48 h ( A ) or 72 h ( B ). The cytochrome c level in the mitochondrial or cytosolic fractions was determined using ELISA. Data are shown as mean ± SD from at least 3 replicates. * p < 0.05, ** p < 0.01 vs. vehicle by Dunnett’s test.

Journal: Pharmaceutics

Article Title: Growth Inhibitory Effects of Ester Derivatives of Menahydroquinone-4, the Reduced Form of Vitamin K 2(20) , on All-Trans Retinoic Acid-Resistant HL60 Cell Line

doi: 10.3390/pharmaceutics13050758

Figure Lengend Snippet: Release of cytochrome c in HL60 and HL-60R cells treated with MK-4 and MKH derivatives. HL60 and HL-60R cells treated with the test drugs at 10 µM for 48 h ( A ) or 72 h ( B ). The cytochrome c level in the mitochondrial or cytosolic fractions was determined using ELISA. Data are shown as mean ± SD from at least 3 replicates. * p < 0.05, ** p < 0.01 vs. vehicle by Dunnett’s test.

Article Snippet: The amount of cytochrome c in the mitochondrial and cytosolic fractions was measured using the cytochrome c ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

( A ) Caspase 9 release from NCE control, LST54 and LST421 cells as measured through Caspase-Glo 9 assay kit over 0, 7.5 and 15 µM cisplatin and the positive control etoposide (200 µM). ( B ) ELISA detection of cytochrome c from both cytoplasmic and mitochondrial fractions in LST421, LST54 and NCE cells after cisplatin exposure (7.5 µM). ( C ) Comparison of the ratio of mitochondrial:cytoplasmic cytochrome c after exposure to cisplatin (7.5 µM). ( D ) Cytochrome c release from mitochondria using Mitotracker green and FITC 568 secondary labelled cytochrome c . ( E ) Mitochondrial depolarisation measured using JC-1 fluorophore in the NCE control and LST421 after exposure to FCCP (20 nM) and cisplatin (7.5 µM). Unpaired t -test used to determine statistical significance. * indicates a p < 0.05. ns = non-significant.

Journal: Scientific Reports

Article Title: Cytoglobin protects cancer cells from apoptosis by regulation of mitochondrial cardiolipin

doi: 10.1038/s41598-020-79830-w

Figure Lengend Snippet: ( A ) Caspase 9 release from NCE control, LST54 and LST421 cells as measured through Caspase-Glo 9 assay kit over 0, 7.5 and 15 µM cisplatin and the positive control etoposide (200 µM). ( B ) ELISA detection of cytochrome c from both cytoplasmic and mitochondrial fractions in LST421, LST54 and NCE cells after cisplatin exposure (7.5 µM). ( C ) Comparison of the ratio of mitochondrial:cytoplasmic cytochrome c after exposure to cisplatin (7.5 µM). ( D ) Cytochrome c release from mitochondria using Mitotracker green and FITC 568 secondary labelled cytochrome c . ( E ) Mitochondrial depolarisation measured using JC-1 fluorophore in the NCE control and LST421 after exposure to FCCP (20 nM) and cisplatin (7.5 µM). Unpaired t -test used to determine statistical significance. * indicates a p < 0.05. ns = non-significant.

Article Snippet: Cytochrome c was detected in both fractions of each sample using a cytochrome c ELISA kit (Novus Biologicals) according to the manufacturer’s instructions.

Techniques: Positive Control, Enzyme-linked Immunosorbent Assay, Comparison