myotube differentiation  (ATCC)


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    ATCC myotube differentiation
    Effects of PYCP on the expression of TNF-R1 in TNF-α-treated C2C12 <t>myotubes.</t> TNF-R1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P
    Myotube Differentiation, supplied by ATCC, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/myotube differentiation/product/ATCC
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    myotube differentiation - by Bioz Stars, 2022-10
    80/100 stars

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    1) Product Images from "Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway"

    Article Title: Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2021.12125

    Effects of PYCP on the expression of TNF-R1 in TNF-α-treated C2C12 myotubes. TNF-R1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P
    Figure Legend Snippet: Effects of PYCP on the expression of TNF-R1 in TNF-α-treated C2C12 myotubes. TNF-R1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Techniques Used: Expressing, Western Blot, Standard Deviation

    Effects of PYCP on the production of IL-6 in TNF-α-treated C2C12 myotubes. C2C12 myotubes were treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. IL-6 in the culture media was measured using ELISA. Data are presented as the mean ± standard deviation of three independent experiments. *P
    Figure Legend Snippet: Effects of PYCP on the production of IL-6 in TNF-α-treated C2C12 myotubes. C2C12 myotubes were treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. IL-6 in the culture media was measured using ELISA. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Techniques Used: Enzyme-linked Immunosorbent Assay, Standard Deviation

    Effects of PYCP on the ubiquitin-proteasome system in TNF-α-treated C2C12 myotubes. (A) The atrogin-1/MAFbx and MuRF1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. (B) 20S proteasome activity was assessed by detecting AMC in cell lysates after cleavage from the AMC-tagged peptide LLVY. Data are presented as the mean ± standard deviation of three independent experiments. *P
    Figure Legend Snippet: Effects of PYCP on the ubiquitin-proteasome system in TNF-α-treated C2C12 myotubes. (A) The atrogin-1/MAFbx and MuRF1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. (B) 20S proteasome activity was assessed by detecting AMC in cell lysates after cleavage from the AMC-tagged peptide LLVY. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Techniques Used: Expressing, Western Blot, Activity Assay, Standard Deviation

    Effects of TNF-α and PYCP on the cytotoxicity of C2C12 myotubes. (A) The cell viability of C2C12 myotubes in the presence of 0, 12.5, 25, 50, and 100 µg/ml PYCP for 24 h. (B) The cell viability of C2C12 myotubes treated with 25, 50, and 100 µg/ml PYCP for 24 h and cotreated with 20 ng/ml TNF-α for 24 h.*P
    Figure Legend Snippet: Effects of TNF-α and PYCP on the cytotoxicity of C2C12 myotubes. (A) The cell viability of C2C12 myotubes in the presence of 0, 12.5, 25, 50, and 100 µg/ml PYCP for 24 h. (B) The cell viability of C2C12 myotubes treated with 25, 50, and 100 µg/ml PYCP for 24 h and cotreated with 20 ng/ml TNF-α for 24 h.*P

    Techniques Used:

    Effects of PYCP on the expression of MyoD and myogenin in TNF-α-treated C2C12 myotubes. MyoD and myogenin protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P
    Figure Legend Snippet: Effects of PYCP on the expression of MyoD and myogenin in TNF-α-treated C2C12 myotubes. MyoD and myogenin protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Techniques Used: Expressing, Western Blot, Standard Deviation

    Effects of PYCP on the production of intracellular ROS in TNF-α-treated C2C12 myotubes. C2C12 myotubes were treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Intracellular ROS production was measured using DCF-DA and fluorescence intensity analysis. Data are presented as the mean ± standard deviation of three independent experiments. *P
    Figure Legend Snippet: Effects of PYCP on the production of intracellular ROS in TNF-α-treated C2C12 myotubes. C2C12 myotubes were treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Intracellular ROS production was measured using DCF-DA and fluorescence intensity analysis. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Techniques Used: Fluorescence, Standard Deviation

    Effects of PYCP on the activation and translocation of NF-κB/p65 in TNF-α-treated C2C12 myotubes. (A) p-IκBα, IκBα, and NF-κB/p65 protein expression levels of cytosolic fractions were detected by western blot analysis. (B) NF-κB/p65 levels were measured in the nuclear fractions by western blot analysis. β-actin and lamin B1 were used as internal controls for the cytosolic and nuclear fractions, respectively. Data are presented as the mean ± standard deviation of three independent experiments. *P
    Figure Legend Snippet: Effects of PYCP on the activation and translocation of NF-κB/p65 in TNF-α-treated C2C12 myotubes. (A) p-IκBα, IκBα, and NF-κB/p65 protein expression levels of cytosolic fractions were detected by western blot analysis. (B) NF-κB/p65 levels were measured in the nuclear fractions by western blot analysis. β-actin and lamin B1 were used as internal controls for the cytosolic and nuclear fractions, respectively. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Techniques Used: Activation Assay, Translocation Assay, Expressing, Western Blot, Standard Deviation

    Effects of PYCP on myotube diameter in TNF-α-treated C2C12 myotubes. Representative images and quantification of myotube diameters are shown for C2C12 myotubes treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Images were captured at ×20 magnification (scale bar, 50 µm). Data are presented as the mean ± standard deviation of three independent experiments. *P
    Figure Legend Snippet: Effects of PYCP on myotube diameter in TNF-α-treated C2C12 myotubes. Representative images and quantification of myotube diameters are shown for C2C12 myotubes treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Images were captured at ×20 magnification (scale bar, 50 µm). Data are presented as the mean ± standard deviation of three independent experiments. *P

    Techniques Used: Standard Deviation

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    ATCC myotube differentiation
    Effects of PYCP on the expression of TNF-R1 in TNF-α-treated C2C12 <t>myotubes.</t> TNF-R1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P
    Myotube Differentiation, supplied by ATCC, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/myotube differentiation/product/ATCC
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    myotube differentiation - by Bioz Stars, 2022-10
    80/100 stars
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    Effects of PYCP on the expression of TNF-R1 in TNF-α-treated C2C12 myotubes. TNF-R1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway

    doi: 10.3892/mmr.2021.12125

    Figure Lengend Snippet: Effects of PYCP on the expression of TNF-R1 in TNF-α-treated C2C12 myotubes. TNF-R1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Article Snippet: When myoblasts were approximately 80–90% confluent, myotube differentiation was initiated by replacing the growth medium with differentiation medium: DMEM supplemented with 2% FBS.

    Techniques: Expressing, Western Blot, Standard Deviation

    Effects of PYCP on the production of IL-6 in TNF-α-treated C2C12 myotubes. C2C12 myotubes were treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. IL-6 in the culture media was measured using ELISA. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway

    doi: 10.3892/mmr.2021.12125

    Figure Lengend Snippet: Effects of PYCP on the production of IL-6 in TNF-α-treated C2C12 myotubes. C2C12 myotubes were treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. IL-6 in the culture media was measured using ELISA. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Article Snippet: When myoblasts were approximately 80–90% confluent, myotube differentiation was initiated by replacing the growth medium with differentiation medium: DMEM supplemented with 2% FBS.

    Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

    Effects of PYCP on the ubiquitin-proteasome system in TNF-α-treated C2C12 myotubes. (A) The atrogin-1/MAFbx and MuRF1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. (B) 20S proteasome activity was assessed by detecting AMC in cell lysates after cleavage from the AMC-tagged peptide LLVY. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway

    doi: 10.3892/mmr.2021.12125

    Figure Lengend Snippet: Effects of PYCP on the ubiquitin-proteasome system in TNF-α-treated C2C12 myotubes. (A) The atrogin-1/MAFbx and MuRF1 protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. (B) 20S proteasome activity was assessed by detecting AMC in cell lysates after cleavage from the AMC-tagged peptide LLVY. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Article Snippet: When myoblasts were approximately 80–90% confluent, myotube differentiation was initiated by replacing the growth medium with differentiation medium: DMEM supplemented with 2% FBS.

    Techniques: Expressing, Western Blot, Activity Assay, Standard Deviation

    Effects of TNF-α and PYCP on the cytotoxicity of C2C12 myotubes. (A) The cell viability of C2C12 myotubes in the presence of 0, 12.5, 25, 50, and 100 µg/ml PYCP for 24 h. (B) The cell viability of C2C12 myotubes treated with 25, 50, and 100 µg/ml PYCP for 24 h and cotreated with 20 ng/ml TNF-α for 24 h.*P

    Journal: Molecular Medicine Reports

    Article Title: Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway

    doi: 10.3892/mmr.2021.12125

    Figure Lengend Snippet: Effects of TNF-α and PYCP on the cytotoxicity of C2C12 myotubes. (A) The cell viability of C2C12 myotubes in the presence of 0, 12.5, 25, 50, and 100 µg/ml PYCP for 24 h. (B) The cell viability of C2C12 myotubes treated with 25, 50, and 100 µg/ml PYCP for 24 h and cotreated with 20 ng/ml TNF-α for 24 h.*P

    Article Snippet: When myoblasts were approximately 80–90% confluent, myotube differentiation was initiated by replacing the growth medium with differentiation medium: DMEM supplemented with 2% FBS.

    Techniques:

    Effects of PYCP on the expression of MyoD and myogenin in TNF-α-treated C2C12 myotubes. MyoD and myogenin protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway

    doi: 10.3892/mmr.2021.12125

    Figure Lengend Snippet: Effects of PYCP on the expression of MyoD and myogenin in TNF-α-treated C2C12 myotubes. MyoD and myogenin protein expression levels were detected by western blot analysis. GAPDH was used as an internal standard. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Article Snippet: When myoblasts were approximately 80–90% confluent, myotube differentiation was initiated by replacing the growth medium with differentiation medium: DMEM supplemented with 2% FBS.

    Techniques: Expressing, Western Blot, Standard Deviation

    Effects of PYCP on the production of intracellular ROS in TNF-α-treated C2C12 myotubes. C2C12 myotubes were treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Intracellular ROS production was measured using DCF-DA and fluorescence intensity analysis. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway

    doi: 10.3892/mmr.2021.12125

    Figure Lengend Snippet: Effects of PYCP on the production of intracellular ROS in TNF-α-treated C2C12 myotubes. C2C12 myotubes were treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Intracellular ROS production was measured using DCF-DA and fluorescence intensity analysis. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Article Snippet: When myoblasts were approximately 80–90% confluent, myotube differentiation was initiated by replacing the growth medium with differentiation medium: DMEM supplemented with 2% FBS.

    Techniques: Fluorescence, Standard Deviation

    Effects of PYCP on the activation and translocation of NF-κB/p65 in TNF-α-treated C2C12 myotubes. (A) p-IκBα, IκBα, and NF-κB/p65 protein expression levels of cytosolic fractions were detected by western blot analysis. (B) NF-κB/p65 levels were measured in the nuclear fractions by western blot analysis. β-actin and lamin B1 were used as internal controls for the cytosolic and nuclear fractions, respectively. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway

    doi: 10.3892/mmr.2021.12125

    Figure Lengend Snippet: Effects of PYCP on the activation and translocation of NF-κB/p65 in TNF-α-treated C2C12 myotubes. (A) p-IκBα, IκBα, and NF-κB/p65 protein expression levels of cytosolic fractions were detected by western blot analysis. (B) NF-κB/p65 levels were measured in the nuclear fractions by western blot analysis. β-actin and lamin B1 were used as internal controls for the cytosolic and nuclear fractions, respectively. Data are presented as the mean ± standard deviation of three independent experiments. *P

    Article Snippet: When myoblasts were approximately 80–90% confluent, myotube differentiation was initiated by replacing the growth medium with differentiation medium: DMEM supplemented with 2% FBS.

    Techniques: Activation Assay, Translocation Assay, Expressing, Western Blot, Standard Deviation

    Effects of PYCP on myotube diameter in TNF-α-treated C2C12 myotubes. Representative images and quantification of myotube diameters are shown for C2C12 myotubes treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Images were captured at ×20 magnification (scale bar, 50 µm). Data are presented as the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Pyropia yezoensis protein protects against TNF-α-induced myotube atrophy in C2C12 myotubes via the NF-κB signaling pathway

    doi: 10.3892/mmr.2021.12125

    Figure Lengend Snippet: Effects of PYCP on myotube diameter in TNF-α-treated C2C12 myotubes. Representative images and quantification of myotube diameters are shown for C2C12 myotubes treated with 20 ng/ml TNF-α and PYCP (25, 50, or 100 µg/ml) for 48 h. Images were captured at ×20 magnification (scale bar, 50 µm). Data are presented as the mean ± standard deviation of three independent experiments. *P

    Article Snippet: When myoblasts were approximately 80–90% confluent, myotube differentiation was initiated by replacing the growth medium with differentiation medium: DMEM supplemented with 2% FBS.

    Techniques: Standard Deviation