Journal: Aging (Albany NY)

Article Title: Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old rats

doi: 10.18632/aging.103960

Figure Lengend Snippet: Evaluation of the mitochondrial potential, anti-apoptotic and pro-apoptotic proteins, the intensity of mitochondrial biogenesis, and the levels of mitochondrial deacetylase SIRT-3. ( A ) Confocal microscopy of vital kidney slices loaded with mitochondrial probe TMRE. Scale bar, 100 μm; ( B ) Mitochondrial membrane potential estimated by TMRE fluorescence intensity normalized to MitoTracker Green fluorescence intensity; ( C , D ) Levels of Bcl-X L in kidney tissue of young and old rats after DR; ( E , F ) Levels of Bcl-X L in kidneys of young and old rats in response to I/R; ( G ) Comparison of Bcl-XL levels in kidney tissue of young and old rats; ( H , I ) Levels of Bcl-X s in kidney tissue of young and old rats after the DR; ( J , K ) Levels of Bcl-X s in kidney tissue of young and old rats in response to I/R; ( L ) Comparison of Bcl-XS levels in kidney tissue of young and old rats; ( M , N ) Levels of SIRT-3 in kidney tissue of young and old rats after the DR; ( O , P ) Levels of PGC-1α in kidney tissue of young and old rats after the DR. *p < 0.05 compared to AL-group. For young AL rats n=5, for young DR rats n=6, for old AL rats n=6, for old DR rats n=5. AL, ad libitum, DR, dietary restriction, IR, ischemia/reperfusion.

Article Snippet: Membranes were blocked with 5% blocking agent (Amersham Pharmacia Biotech, RPN2125V, UK) in TBS containing 0.05% Tween-20 and subsequently incubated with primary antibodies: anti-α-smooth muscle actin rabbit 1:1000 (Abcam, UK), anti-PCNA 1:1000 rabbit (Cell Signaling, USA), anti-LC3 A/B rabbit 1:1000 (Cell Signaling, USA), anti-LAMP-1 rabbit 1:1000 (Abcam, UK), anti-p62/SQSTM1 rabbit 1:1000 (Cell Signaling, USA), anti-Bcl-X rabbit 1:1000 (Cell Signaling, USA), anti-SIRT-3 rabbit 1:1000 (Cell Signaling, USA), anti-PGC-1α rabbit 1:1000 (Invitrogen, Thermo Fisher Scientific, USA), anti-β-actin mouse 1:2000 (Sigma, USA).

Techniques: Histone Deacetylase Assay, Confocal Microscopy, Fluorescence

Journal: Oncotarget

Article Title: Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma

doi: 10.18632/oncotarget.10226

Figure Lengend Snippet: (A) Expression of Lewis y antigen, ANXA4, and NF-kB p50 in cells before and after treatment; (B) Expression of Lewis y antigen and NF-kB p50 on ANXA4 before and after transfection, detected by IP; (C) Expression of p21 in cells before and after transfection; (D) Changes in expression of integrin α5, β1, β5, MMP2, MMP9, LC3, and Bcl-1 before and after treatment, detected by WB.

Article Snippet: Antibodies used were: rabbit anti-human ANXA4 (1:200, Santa Cruz), mouse anti-human ANXA4 (1:1000, Abcam), mouse anti-human Lewis y antigen(1:500, Abcam), rabbit anti-human NF- KB p50 (1:200, Santa Cruz), mouse anti-human NF-kB p50 (1:200, Santa Cruz), rabbit anti-human integrin α5 (1:500, Proteintech, Wuhan, China), rabbit anti-human integrin β1 (1:500, Proteintech), rabbit anti-human integrin β5 (1:200, Beijing Biosynthesis Biotechnology Co., Ltd., Beijing, China), rabbit anti-human MMP2 (1:500, Proteintech), rabbit anti-human MMP9 (1:500, Proteintech), rabbit anti-human LC-3 (1:1000, Abcam), and rabbit anti-human Bcl-1 (1:1000, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Transfection

Journal: Frontiers in Microbiology

Article Title: A New Role for Helicobacter pylori Urease: Contributions to Angiogenesis

doi: 10.3389/fmicb.2017.01883

Figure Lengend Snippet: Helicobacter pylori urease is not cytotoxic to gastric epithelial cell lines. (A) Effect of HPU in cell proliferation and viability of AGS, Kato-III, and MKN28 cell lines incubated with 300 nM HPU or PBS for 24 h, was evaluated by the MTT assay. (B) Levels of pro- and anti-apoptotic proteins in AGS cells treated with daily freshly added 300 nM HPU for 6 h (“acute” exposure), and for 48 h (“chronic” exposure). (C) Cell lysates were analyzed for the cytoplasmic content of BAD (left panel) and Bcl-X L (right panel) by Western blot. Results shown are means ± SEM, N = 3; ∗ p ≤ 0.05 was considered statistically significant.

Article Snippet: Membranes were blocked with Tween-Tris buffered saline (TBS) (20 mM Tris-HCl, pH 7.5, 500 mM NaCl, 0.1% Tween-20) containing 1% bovine serum albumin (BSA) and probed with rabbit monoclonal antibodies: anti-Bcl-X L (Cell Signaling Technology, Danvers, MA, United States, 1:500) and anti-BAD (Cell Signaling Technology, 1:500).

Techniques: Incubation, MTT Assay, Western Blot