beauveria bassiana  (ATCC)


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    ATCC beauveria bassiana
    SDS-PAGE analysis of B. <t>bassiana</t> recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).
    Beauveria Bassiana, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana"

    Article Title: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana

    Journal: Clinical and Molecular Allergy

    doi: 10.1186/1476-7961-4-12

    SDS-PAGE analysis of B. bassiana recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).
    Figure Legend Snippet: SDS-PAGE analysis of B. bassiana recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).

    Techniques Used: SDS Page, Recombinant, Staining, Expressing, Plasmid Preparation, Construct

    SDS-PAGE analysis of soluble and pellet (inclusion bodies) fractions of the B. bassiana proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained extracts of soluble fractions lanes 1), 3), 5) and 7), and pellet fractions, lanes 2), 4), 6), and 8). Expression of Bb-Eno1, lanes 1) and 2), Bb-f2, lanes 3) and 4), Bb-Ald, lanes 5) and 6), and Bb-Hex, lanes 7) and 8).
    Figure Legend Snippet: SDS-PAGE analysis of soluble and pellet (inclusion bodies) fractions of the B. bassiana proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained extracts of soluble fractions lanes 1), 3), 5) and 7), and pellet fractions, lanes 2), 4), 6), and 8). Expression of Bb-Eno1, lanes 1) and 2), Bb-f2, lanes 3) and 4), Bb-Ald, lanes 5) and 6), and Bb-Hex, lanes 7) and 8).

    Techniques Used: SDS Page, Staining, Expressing

    SDS-PAGE analysis of the temperature sensitivity of the recombinant B. bassiana proteins. SDS-PAGE, Coomasie Blue stained, E. coli crude extracts subjected to; 1 min heat denaturation at 95°C, panel A), 5 min, 95°C, panel B), and 20 min, 95°C, panel C), Lanes correspond to crude extracts containing, lane 1) Bb-Eno1, lane 2) Bb-f2, lane 3) Bb-Ald, and lane 4) Bb-Hex.
    Figure Legend Snippet: SDS-PAGE analysis of the temperature sensitivity of the recombinant B. bassiana proteins. SDS-PAGE, Coomasie Blue stained, E. coli crude extracts subjected to; 1 min heat denaturation at 95°C, panel A), 5 min, 95°C, panel B), and 20 min, 95°C, panel C), Lanes correspond to crude extracts containing, lane 1) Bb-Eno1, lane 2) Bb-f2, lane 3) Bb-Ald, and lane 4) Bb-Hex.

    Techniques Used: SDS Page, Recombinant, Staining

    Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pooled from (10 each) patients displaying mold allergies as indicated on the panels (A-J, and K-T). The final concentration of individual sera in each pool was 1:35. An HRP conjugated goat anti-human IgE antibody was used as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, 4) Bb-Hex. Lane 5) 40 μg crude B. bassiana extract.
    Figure Legend Snippet: Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pooled from (10 each) patients displaying mold allergies as indicated on the panels (A-J, and K-T). The final concentration of individual sera in each pool was 1:35. An HRP conjugated goat anti-human IgE antibody was used as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, 4) Bb-Hex. Lane 5) 40 μg crude B. bassiana extract.

    Techniques Used: Western Blot, Recombinant, Concentration Assay

    Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pools (2 each) as indicated on the panels (AB, CD, EF, GH, and IJ), with a 1:5 final concentration of individual sera in each pool. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald.
    Figure Legend Snippet: Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pools (2 each) as indicated on the panels (AB, CD, EF, GH, and IJ), with a 1:5 final concentration of individual sera in each pool. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald.

    Techniques Used: Western Blot, Recombinant, Concentration Assay

    Ponceau S staining and immunoblot analysis of recombinant B. bassiana . Immunoblots were probed with individual sera, A) and B) as indicated on the panels using a 1:5 final concentration of sera. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, and 4) Bb-Hex. Panel 1) represents Ponceau S staining of the PVDF membrane after transfer.
    Figure Legend Snippet: Ponceau S staining and immunoblot analysis of recombinant B. bassiana . Immunoblots were probed with individual sera, A) and B) as indicated on the panels using a 1:5 final concentration of sera. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, and 4) Bb-Hex. Panel 1) represents Ponceau S staining of the PVDF membrane after transfer.

    Techniques Used: Staining, Western Blot, Recombinant, Concentration Assay

    beauveria bassiana  (ATCC)


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    ATCC beauveria bassiana
    SDS-PAGE analysis of B. <t>bassiana</t> recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).
    Beauveria Bassiana, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    beauveria bassiana - by Bioz Stars, 2024-02
    92/100 stars

    Images

    1) Product Images from "Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana"

    Article Title: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana

    Journal: Clinical and Molecular Allergy

    doi: 10.1186/1476-7961-4-12

    SDS-PAGE analysis of B. bassiana recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).
    Figure Legend Snippet: SDS-PAGE analysis of B. bassiana recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).

    Techniques Used: SDS Page, Recombinant, Staining, Expressing, Plasmid Preparation, Construct

    SDS-PAGE analysis of soluble and pellet (inclusion bodies) fractions of the B. bassiana proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained extracts of soluble fractions lanes 1), 3), 5) and 7), and pellet fractions, lanes 2), 4), 6), and 8). Expression of Bb-Eno1, lanes 1) and 2), Bb-f2, lanes 3) and 4), Bb-Ald, lanes 5) and 6), and Bb-Hex, lanes 7) and 8).
    Figure Legend Snippet: SDS-PAGE analysis of soluble and pellet (inclusion bodies) fractions of the B. bassiana proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained extracts of soluble fractions lanes 1), 3), 5) and 7), and pellet fractions, lanes 2), 4), 6), and 8). Expression of Bb-Eno1, lanes 1) and 2), Bb-f2, lanes 3) and 4), Bb-Ald, lanes 5) and 6), and Bb-Hex, lanes 7) and 8).

    Techniques Used: SDS Page, Staining, Expressing

    SDS-PAGE analysis of the temperature sensitivity of the recombinant B. bassiana proteins. SDS-PAGE, Coomasie Blue stained, E. coli crude extracts subjected to; 1 min heat denaturation at 95°C, panel A), 5 min, 95°C, panel B), and 20 min, 95°C, panel C), Lanes correspond to crude extracts containing, lane 1) Bb-Eno1, lane 2) Bb-f2, lane 3) Bb-Ald, and lane 4) Bb-Hex.
    Figure Legend Snippet: SDS-PAGE analysis of the temperature sensitivity of the recombinant B. bassiana proteins. SDS-PAGE, Coomasie Blue stained, E. coli crude extracts subjected to; 1 min heat denaturation at 95°C, panel A), 5 min, 95°C, panel B), and 20 min, 95°C, panel C), Lanes correspond to crude extracts containing, lane 1) Bb-Eno1, lane 2) Bb-f2, lane 3) Bb-Ald, and lane 4) Bb-Hex.

    Techniques Used: SDS Page, Recombinant, Staining

    Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pooled from (10 each) patients displaying mold allergies as indicated on the panels (A-J, and K-T). The final concentration of individual sera in each pool was 1:35. An HRP conjugated goat anti-human IgE antibody was used as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, 4) Bb-Hex. Lane 5) 40 μg crude B. bassiana extract.
    Figure Legend Snippet: Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pooled from (10 each) patients displaying mold allergies as indicated on the panels (A-J, and K-T). The final concentration of individual sera in each pool was 1:35. An HRP conjugated goat anti-human IgE antibody was used as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, 4) Bb-Hex. Lane 5) 40 μg crude B. bassiana extract.

    Techniques Used: Western Blot, Recombinant, Concentration Assay

    Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pools (2 each) as indicated on the panels (AB, CD, EF, GH, and IJ), with a 1:5 final concentration of individual sera in each pool. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald.
    Figure Legend Snippet: Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pools (2 each) as indicated on the panels (AB, CD, EF, GH, and IJ), with a 1:5 final concentration of individual sera in each pool. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald.

    Techniques Used: Western Blot, Recombinant, Concentration Assay

    Ponceau S staining and immunoblot analysis of recombinant B. bassiana . Immunoblots were probed with individual sera, A) and B) as indicated on the panels using a 1:5 final concentration of sera. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, and 4) Bb-Hex. Panel 1) represents Ponceau S staining of the PVDF membrane after transfer.
    Figure Legend Snippet: Ponceau S staining and immunoblot analysis of recombinant B. bassiana . Immunoblots were probed with individual sera, A) and B) as indicated on the panels using a 1:5 final concentration of sera. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, and 4) Bb-Hex. Panel 1) represents Ponceau S staining of the PVDF membrane after transfer.

    Techniques Used: Staining, Western Blot, Recombinant, Concentration Assay

    beauveria bassiana  (ATCC)


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    ATCC beauveria bassiana
    SDS-PAGE and immunoblot analysis of <t>Beauveria</t> <t>bassiana</t> crude extracts. SDS-PAGE, Gelcode blue stained, lanes A) 5 μg protein standards, and B) 40 μg B. bassiana crude extract. Immunoblots probed with pooled serum mix-I (patients displaying mould allergies), lanes 1), 5 μg protein standards, 2) 20 μg B. bassiana crude extract, 3) 40 μg crude extract, 4) 40 μg crude extract, Proteinase K treated, 5) 40 μg crude extract, denaturing buffer control (no EndoH), 6) 40 μg crude extract, EndoH treated
    Beauveria Bassiana, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    beauveria bassiana - by Bioz Stars, 2024-02
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    1) Product Images from "Allergens of the entomopathogenic fungus Beauveria bassiana"

    Article Title: Allergens of the entomopathogenic fungus Beauveria bassiana

    Journal: Clinical and molecular allergy : CMA

    doi: 10.1186/1476-7961-3-1

    SDS-PAGE and immunoblot analysis of Beauveria bassiana crude extracts. SDS-PAGE, Gelcode blue stained, lanes A) 5 μg protein standards, and B) 40 μg B. bassiana crude extract. Immunoblots probed with pooled serum mix-I (patients displaying mould allergies), lanes 1), 5 μg protein standards, 2) 20 μg B. bassiana crude extract, 3) 40 μg crude extract, 4) 40 μg crude extract, Proteinase K treated, 5) 40 μg crude extract, denaturing buffer control (no EndoH), 6) 40 μg crude extract, EndoH treated
    Figure Legend Snippet: SDS-PAGE and immunoblot analysis of Beauveria bassiana crude extracts. SDS-PAGE, Gelcode blue stained, lanes A) 5 μg protein standards, and B) 40 μg B. bassiana crude extract. Immunoblots probed with pooled serum mix-I (patients displaying mould allergies), lanes 1), 5 μg protein standards, 2) 20 μg B. bassiana crude extract, 3) 40 μg crude extract, 4) 40 μg crude extract, Proteinase K treated, 5) 40 μg crude extract, denaturing buffer control (no EndoH), 6) 40 μg crude extract, EndoH treated

    Techniques Used: SDS Page, Western Blot, Staining

    Immunoprint analysis of B. bassiana extracts (40 μg crude extract/strip) probed with individual sera. Lane 1) 5 μg protein standards, 2) pooled serum mix-II (patients displaying mould allergies). Lanes A)–G) membrane strips treated with individual sera, Lanes H)–M) membrane strips probed with individuals having had occupational exposure to B. bassiana and other fungi (see intra-dermal skin test results for individuals J–M, Table 2).
    Figure Legend Snippet: Immunoprint analysis of B. bassiana extracts (40 μg crude extract/strip) probed with individual sera. Lane 1) 5 μg protein standards, 2) pooled serum mix-II (patients displaying mould allergies). Lanes A)–G) membrane strips treated with individual sera, Lanes H)–M) membrane strips probed with individuals having had occupational exposure to B. bassiana and other fungi (see intra-dermal skin test results for individuals J–M, Table 2).

    Techniques Used: Stripping Membranes

    IgE immunoblot inhibition with fungi. B. bassiana protein strips (40 μg crude extract) were blocked and incubated with mix containing (500 μl) pooled sera (mix-II) and 2) no additions, 3) 40 μg Alternaria alternata crude extract, 4) 400 μg Alternaria alternata , 5) 40 μg Aspergillus fumigatus , 6) 400 μg Aspergillus fumigatus , 7) 400 μg Cladosporium herbarum , 8) 400 μg Candida albicans , 9) 400 μg Epicoccum purpurascens , and 10) 400 μg Penicillium notatum protein.
    Figure Legend Snippet: IgE immunoblot inhibition with fungi. B. bassiana protein strips (40 μg crude extract) were blocked and incubated with mix containing (500 μl) pooled sera (mix-II) and 2) no additions, 3) 40 μg Alternaria alternata crude extract, 4) 400 μg Alternaria alternata , 5) 40 μg Aspergillus fumigatus , 6) 400 μg Aspergillus fumigatus , 7) 400 μg Cladosporium herbarum , 8) 400 μg Candida albicans , 9) 400 μg Epicoccum purpurascens , and 10) 400 μg Penicillium notatum protein.

    Techniques Used: Western Blot, Inhibition, Incubation

    Intradermal skin test results using B. bassiana extract
    Figure Legend Snippet: Intradermal skin test results using B. bassiana extract

    Techniques Used:

    strain atcc 90517  (ATCC)


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    ATCC strain atcc 90517
    Strain Atcc 90517, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    strain atcc 90517  (ATCC)


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    ATCC strain atcc 90517
    Strain Atcc 90517, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    conspicuous phenotype  (ATCC)


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    ATCC conspicuous phenotype
    Conspicuous Phenotype, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b bassiana  (ATCC)


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    ATCC b bassiana
    B Bassiana, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b bassiana atcc 90517  (ATCC)


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    ATCC b bassiana atcc 90517
    MICs of BbAFP1 and BbAFP1 F50A for selected microorganisms.
    B Bassiana Atcc 90517, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Characterization of a fungal competition factor: Production of a conidial cell-wall associated antifungal peptide"

    Article Title: Characterization of a fungal competition factor: Production of a conidial cell-wall associated antifungal peptide

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1008518

    MICs of BbAFP1 and BbAFP1 F50A for selected microorganisms.
    Figure Legend Snippet: MICs of BbAFP1 and BbAFP1 F50A for selected microorganisms.

    Techniques Used:

    b bassiana atcc 90517  (ATCC)


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    ATCC b bassiana atcc 90517
    B Bassiana Atcc 90517, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b bassiana atcc 90517  (ATCC)


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    ATCC b bassiana atcc 90517
    B Bassiana Atcc 90517, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b bassiana wild type strains bb0062  (ATCC)


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    ATCC b bassiana wild type strains bb0062
    Transcriptional profiles of B. bassiana β-1,3-glucanosyltransferase genes. (A) Expression of Bbgas1 to Bbgas6 in <t>wild-type</t> B. bassiana as a function of external pH. (B) Expression of Bbgas3 and PacC in wild-type B. bassiana. (C) Expression of Bbgas3 in a B. bassiana ΔBbpacC background strain as a function of pH. Error bars are the standard deviation (SD) of the mean of the results from three replicate assays. Asterisks indicate significant differences.
    B Bassiana Wild Type Strains Bb0062, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions"

    Article Title: The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01086-18

    Transcriptional profiles of B. bassiana β-1,3-glucanosyltransferase genes. (A) Expression of Bbgas1 to Bbgas6 in wild-type B. bassiana as a function of external pH. (B) Expression of Bbgas3 and PacC in wild-type B. bassiana. (C) Expression of Bbgas3 in a B. bassiana ΔBbpacC background strain as a function of pH. Error bars are the standard deviation (SD) of the mean of the results from three replicate assays. Asterisks indicate significant differences.
    Figure Legend Snippet: Transcriptional profiles of B. bassiana β-1,3-glucanosyltransferase genes. (A) Expression of Bbgas1 to Bbgas6 in wild-type B. bassiana as a function of external pH. (B) Expression of Bbgas3 and PacC in wild-type B. bassiana. (C) Expression of Bbgas3 in a B. bassiana ΔBbpacC background strain as a function of pH. Error bars are the standard deviation (SD) of the mean of the results from three replicate assays. Asterisks indicate significant differences.

    Techniques Used: Expressing, Standard Deviation

    Growth of wild type (WT), ΔBbgas3 mutant, and complemented (ΔBbgas3::Bbgas3) strains on PDA buffered to indicated starting pH values, as described Materials and Methods. Plates were spot inoculated with a conidial suspension of each strain and incubated at 26°C for 7 days. (B) Quantitative real-time PCR (qRT-PCR) expression profile of Bbgas genes (1,–6) in the B. bassiana ΔBbgas3 mutant as a function of the pH of the growth media. Fungal cultures were grown RNA extracted as detailed in Materials and Methods. Error bars are the SD of the mean of the results from three replicate assays; asterisks indicate significant differences (Tukey's HSD, P < 0.01).
    Figure Legend Snippet: Growth of wild type (WT), ΔBbgas3 mutant, and complemented (ΔBbgas3::Bbgas3) strains on PDA buffered to indicated starting pH values, as described Materials and Methods. Plates were spot inoculated with a conidial suspension of each strain and incubated at 26°C for 7 days. (B) Quantitative real-time PCR (qRT-PCR) expression profile of Bbgas genes (1,–6) in the B. bassiana ΔBbgas3 mutant as a function of the pH of the growth media. Fungal cultures were grown RNA extracted as detailed in Materials and Methods. Error bars are the SD of the mean of the results from three replicate assays; asterisks indicate significant differences (Tukey's HSD, P < 0.01).

    Techniques Used: Mutagenesis, Incubation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing

    Effect of Bbgas3 deletion on B. bassiana cell wall components. (A) Total carbohydrate, alkaline-soluble and -insoluble, and chitin content of B. bassiana wild-type and ΔBbgas3 mutant cells grown in media buffered to pH 7 and pH 10, and analyzed as detailed in Materials and Methods. Error bars are the SD of the mean of the results from three replicate assays, and asterisks on column bars indicate significant difference (Tukey's HSD, P < 0.01). (B) Calcofluor white staining of wild-type and ΔBbgas3 mutant cells. Inset and arrow indicate enhanced chitin deposition in the hyphal tip of the mutant at pH. Scale bar = 500 μm.
    Figure Legend Snippet: Effect of Bbgas3 deletion on B. bassiana cell wall components. (A) Total carbohydrate, alkaline-soluble and -insoluble, and chitin content of B. bassiana wild-type and ΔBbgas3 mutant cells grown in media buffered to pH 7 and pH 10, and analyzed as detailed in Materials and Methods. Error bars are the SD of the mean of the results from three replicate assays, and asterisks on column bars indicate significant difference (Tukey's HSD, P < 0.01). (B) Calcofluor white staining of wild-type and ΔBbgas3 mutant cells. Inset and arrow indicate enhanced chitin deposition in the hyphal tip of the mutant at pH. Scale bar = 500 μm.

    Techniques Used: Mutagenesis, Staining

    Analysis of conidial cell walls and morphology of the B. bassiana wild type (WT) and ΔBbgas3 mutant. (A and B) TEM images and quantification of conidial cell wall thickness. Scale bar = 0.2 μm. (C and D) Representative images and quantification of distorted conidial morphologies seen in the ΔBbgas3 mutant. Arrows indicate measurement of cell wall thickness. Scale bar = 0.5 μm. Asterisks on column bars indicate significant difference (Tukey's HSD, P < 0.01).
    Figure Legend Snippet: Analysis of conidial cell walls and morphology of the B. bassiana wild type (WT) and ΔBbgas3 mutant. (A and B) TEM images and quantification of conidial cell wall thickness. Scale bar = 0.2 μm. (C and D) Representative images and quantification of distorted conidial morphologies seen in the ΔBbgas3 mutant. Arrows indicate measurement of cell wall thickness. Scale bar = 0.5 μm. Asterisks on column bars indicate significant difference (Tukey's HSD, P < 0.01).

    Techniques Used: Mutagenesis

    Insect bioassays. G. mellonella larvae were treated either topically (A, C, and E) or via intrahemocoel injection (B, D, and F) with conidia from wild-type B. bassiana (■), ΔBbgas3 mutant (●), ΔBbgas3::Bbgas3 complemented (◆) strains, or mock-treated controls (○), as described in Materials and Methods. (A to F) Conidia derived from growth on PDA at pH 5.6 (A and B). 7.0 (C and D), and pH 10 (E and F). The percent survival ± SD over the indicated time course is presented.
    Figure Legend Snippet: Insect bioassays. G. mellonella larvae were treated either topically (A, C, and E) or via intrahemocoel injection (B, D, and F) with conidia from wild-type B. bassiana (■), ΔBbgas3 mutant (●), ΔBbgas3::Bbgas3 complemented (◆) strains, or mock-treated controls (○), as described in Materials and Methods. (A to F) Conidia derived from growth on PDA at pH 5.6 (A and B). 7.0 (C and D), and pH 10 (E and F). The percent survival ± SD over the indicated time course is presented.

    Techniques Used: Injection, Mutagenesis, Derivative Assay

    Flow cytometry distribution and quantification of lectin and β-glucan antibody staining in B. bassiana wild-type (black) and ΔBbgas3 mutant (green) aerial conidia. Error bars are the SD of the mean of the results from three replicate assays. ConA and WGA are the lectins of wheat germ agglutinin and concanavalin A, respectively. Stars on column bars indicate significant differences (Tukey's HSD, P < 0.01).
    Figure Legend Snippet: Flow cytometry distribution and quantification of lectin and β-glucan antibody staining in B. bassiana wild-type (black) and ΔBbgas3 mutant (green) aerial conidia. Error bars are the SD of the mean of the results from three replicate assays. ConA and WGA are the lectins of wheat germ agglutinin and concanavalin A, respectively. Stars on column bars indicate significant differences (Tukey's HSD, P < 0.01).

    Techniques Used: Flow Cytometry, Staining, Mutagenesis

    (A) Growth of B. bassiana wild-type, ΔBbgas3 mutant, and complemented (ΔBbgas3::Bbgas3) strains on CZA containing 0.05% bromocresol purple indicating ambient acidification. (B) Determination of the pH of growth media (CZB) inoculated with each respective fungal strain and measured at the indicated time course. (C) Growth of the wild-type, ΔBbpacC mutant, and ΔBbpacC::Bbgas3 complemented strains are shown on buffered PDA at indicated starting pH values, as described in Materials and Methods. Plates were inoculated with conidial suspensions and incubated at 26°C for 7 days.
    Figure Legend Snippet: (A) Growth of B. bassiana wild-type, ΔBbgas3 mutant, and complemented (ΔBbgas3::Bbgas3) strains on CZA containing 0.05% bromocresol purple indicating ambient acidification. (B) Determination of the pH of growth media (CZB) inoculated with each respective fungal strain and measured at the indicated time course. (C) Growth of the wild-type, ΔBbpacC mutant, and ΔBbpacC::Bbgas3 complemented strains are shown on buffered PDA at indicated starting pH values, as described in Materials and Methods. Plates were inoculated with conidial suspensions and incubated at 26°C for 7 days.

    Techniques Used: Mutagenesis, Incubation

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  • 92
    ATCC beauveria bassiana
    SDS-PAGE analysis of B. <t>bassiana</t> recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).
    Beauveria Bassiana, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC strain atcc 90517
    SDS-PAGE analysis of B. <t>bassiana</t> recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).
    Strain Atcc 90517, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC conspicuous phenotype
    SDS-PAGE analysis of B. <t>bassiana</t> recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).
    Conspicuous Phenotype, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC b bassiana
    SDS-PAGE analysis of B. <t>bassiana</t> recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).
    B Bassiana, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC b bassiana atcc 90517
    MICs of BbAFP1 and BbAFP1 F50A for selected microorganisms.
    B Bassiana Atcc 90517, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC b bassiana wild type strains bb0062
    Transcriptional profiles of B. bassiana β-1,3-glucanosyltransferase genes. (A) Expression of Bbgas1 to Bbgas6 in <t>wild-type</t> B. bassiana as a function of external pH. (B) Expression of Bbgas3 and PacC in wild-type B. bassiana. (C) Expression of Bbgas3 in a B. bassiana ΔBbpacC background strain as a function of pH. Error bars are the standard deviation (SD) of the mean of the results from three replicate assays. Asterisks indicate significant differences.
    B Bassiana Wild Type Strains Bb0062, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SDS-PAGE analysis of B. bassiana recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).

    Journal: Clinical and Molecular Allergy

    Article Title: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana

    doi: 10.1186/1476-7961-4-12

    Figure Lengend Snippet: SDS-PAGE analysis of B. bassiana recombinant proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained, extracts of E. coli strain BL21 harboring pRARE and the indicated expression plasmid constructs; lanes 1) and 2); pET41a:Bb-Eno1, lanes 3) and 4) pET41a:Bb-f2, lanes 5) and 6) pET41a:Bb-Ald, lanes 7) and 8) pET41a:Bb-Hex. Uninduced cell cultures, lanes 1), 3), 5), and 7). IPTG induced cell cultures, lanes 2), 4), 6), and 8).

    Article Snippet: Beauveria bassiana (ATCC 90517) was maintained on Potato dextrose (PD) agar at 26°C.

    Techniques: SDS Page, Recombinant, Staining, Expressing, Plasmid Preparation, Construct

    SDS-PAGE analysis of soluble and pellet (inclusion bodies) fractions of the B. bassiana proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained extracts of soluble fractions lanes 1), 3), 5) and 7), and pellet fractions, lanes 2), 4), 6), and 8). Expression of Bb-Eno1, lanes 1) and 2), Bb-f2, lanes 3) and 4), Bb-Ald, lanes 5) and 6), and Bb-Hex, lanes 7) and 8).

    Journal: Clinical and Molecular Allergy

    Article Title: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana

    doi: 10.1186/1476-7961-4-12

    Figure Lengend Snippet: SDS-PAGE analysis of soluble and pellet (inclusion bodies) fractions of the B. bassiana proteins expressed in E. coli . SDS-PAGE, Coomasie Blue stained extracts of soluble fractions lanes 1), 3), 5) and 7), and pellet fractions, lanes 2), 4), 6), and 8). Expression of Bb-Eno1, lanes 1) and 2), Bb-f2, lanes 3) and 4), Bb-Ald, lanes 5) and 6), and Bb-Hex, lanes 7) and 8).

    Article Snippet: Beauveria bassiana (ATCC 90517) was maintained on Potato dextrose (PD) agar at 26°C.

    Techniques: SDS Page, Staining, Expressing

    SDS-PAGE analysis of the temperature sensitivity of the recombinant B. bassiana proteins. SDS-PAGE, Coomasie Blue stained, E. coli crude extracts subjected to; 1 min heat denaturation at 95°C, panel A), 5 min, 95°C, panel B), and 20 min, 95°C, panel C), Lanes correspond to crude extracts containing, lane 1) Bb-Eno1, lane 2) Bb-f2, lane 3) Bb-Ald, and lane 4) Bb-Hex.

    Journal: Clinical and Molecular Allergy

    Article Title: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana

    doi: 10.1186/1476-7961-4-12

    Figure Lengend Snippet: SDS-PAGE analysis of the temperature sensitivity of the recombinant B. bassiana proteins. SDS-PAGE, Coomasie Blue stained, E. coli crude extracts subjected to; 1 min heat denaturation at 95°C, panel A), 5 min, 95°C, panel B), and 20 min, 95°C, panel C), Lanes correspond to crude extracts containing, lane 1) Bb-Eno1, lane 2) Bb-f2, lane 3) Bb-Ald, and lane 4) Bb-Hex.

    Article Snippet: Beauveria bassiana (ATCC 90517) was maintained on Potato dextrose (PD) agar at 26°C.

    Techniques: SDS Page, Recombinant, Staining

    Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pooled from (10 each) patients displaying mold allergies as indicated on the panels (A-J, and K-T). The final concentration of individual sera in each pool was 1:35. An HRP conjugated goat anti-human IgE antibody was used as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, 4) Bb-Hex. Lane 5) 40 μg crude B. bassiana extract.

    Journal: Clinical and Molecular Allergy

    Article Title: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana

    doi: 10.1186/1476-7961-4-12

    Figure Lengend Snippet: Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pooled from (10 each) patients displaying mold allergies as indicated on the panels (A-J, and K-T). The final concentration of individual sera in each pool was 1:35. An HRP conjugated goat anti-human IgE antibody was used as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, 4) Bb-Hex. Lane 5) 40 μg crude B. bassiana extract.

    Article Snippet: Beauveria bassiana (ATCC 90517) was maintained on Potato dextrose (PD) agar at 26°C.

    Techniques: Western Blot, Recombinant, Concentration Assay

    Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pools (2 each) as indicated on the panels (AB, CD, EF, GH, and IJ), with a 1:5 final concentration of individual sera in each pool. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald.

    Journal: Clinical and Molecular Allergy

    Article Title: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana

    doi: 10.1186/1476-7961-4-12

    Figure Lengend Snippet: Immunoblot analysis of recombinant B. bassiana proteins. Immunoblots were probed with sera pools (2 each) as indicated on the panels (AB, CD, EF, GH, and IJ), with a 1:5 final concentration of individual sera in each pool. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald.

    Article Snippet: Beauveria bassiana (ATCC 90517) was maintained on Potato dextrose (PD) agar at 26°C.

    Techniques: Western Blot, Recombinant, Concentration Assay

    Ponceau S staining and immunoblot analysis of recombinant B. bassiana . Immunoblots were probed with individual sera, A) and B) as indicated on the panels using a 1:5 final concentration of sera. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, and 4) Bb-Hex. Panel 1) represents Ponceau S staining of the PVDF membrane after transfer.

    Journal: Clinical and Molecular Allergy

    Article Title: Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana

    doi: 10.1186/1476-7961-4-12

    Figure Lengend Snippet: Ponceau S staining and immunoblot analysis of recombinant B. bassiana . Immunoblots were probed with individual sera, A) and B) as indicated on the panels using a 1:5 final concentration of sera. Blots were probed with an HRP conjugated goat anti-human IgE antibody as the secondary antibody. Lanes contain recombinant E. coli expressed proteins as follows, lane 1) Bb-Eno1, 2) Bb-f2,. 3) Bb-Ald, and 4) Bb-Hex. Panel 1) represents Ponceau S staining of the PVDF membrane after transfer.

    Article Snippet: Beauveria bassiana (ATCC 90517) was maintained on Potato dextrose (PD) agar at 26°C.

    Techniques: Staining, Western Blot, Recombinant, Concentration Assay

    MICs of BbAFP1 and BbAFP1 F50A for selected microorganisms.

    Journal: PLoS Pathogens

    Article Title: Characterization of a fungal competition factor: Production of a conidial cell-wall associated antifungal peptide

    doi: 10.1371/journal.ppat.1008518

    Figure Lengend Snippet: MICs of BbAFP1 and BbAFP1 F50A for selected microorganisms.

    Article Snippet: B . bassiana ATCC 90517 , > 40 , > 40 , .

    Techniques:

    Transcriptional profiles of B. bassiana β-1,3-glucanosyltransferase genes. (A) Expression of Bbgas1 to Bbgas6 in wild-type B. bassiana as a function of external pH. (B) Expression of Bbgas3 and PacC in wild-type B. bassiana. (C) Expression of Bbgas3 in a B. bassiana ΔBbpacC background strain as a function of pH. Error bars are the standard deviation (SD) of the mean of the results from three replicate assays. Asterisks indicate significant differences.

    Journal: Applied and Environmental Microbiology

    Article Title: The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions

    doi: 10.1128/AEM.01086-18

    Figure Lengend Snippet: Transcriptional profiles of B. bassiana β-1,3-glucanosyltransferase genes. (A) Expression of Bbgas1 to Bbgas6 in wild-type B. bassiana as a function of external pH. (B) Expression of Bbgas3 and PacC in wild-type B. bassiana. (C) Expression of Bbgas3 in a B. bassiana ΔBbpacC background strain as a function of pH. Error bars are the standard deviation (SD) of the mean of the results from three replicate assays. Asterisks indicate significant differences.

    Article Snippet: B. bassiana wild-type strains Bb0062 and ATCC 90517 and various mutants were routinely grown/maintained on potato dextrose broth/agar (PDB/PDA) or Czapek-dox broth/agar (CZB/CZA) (BD Difco, Sparks, MD, USA) at 26°C for 7 to 14 days.

    Techniques: Expressing, Standard Deviation

    Growth of wild type (WT), ΔBbgas3 mutant, and complemented (ΔBbgas3::Bbgas3) strains on PDA buffered to indicated starting pH values, as described Materials and Methods. Plates were spot inoculated with a conidial suspension of each strain and incubated at 26°C for 7 days. (B) Quantitative real-time PCR (qRT-PCR) expression profile of Bbgas genes (1,–6) in the B. bassiana ΔBbgas3 mutant as a function of the pH of the growth media. Fungal cultures were grown RNA extracted as detailed in Materials and Methods. Error bars are the SD of the mean of the results from three replicate assays; asterisks indicate significant differences (Tukey's HSD, P < 0.01).

    Journal: Applied and Environmental Microbiology

    Article Title: The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions

    doi: 10.1128/AEM.01086-18

    Figure Lengend Snippet: Growth of wild type (WT), ΔBbgas3 mutant, and complemented (ΔBbgas3::Bbgas3) strains on PDA buffered to indicated starting pH values, as described Materials and Methods. Plates were spot inoculated with a conidial suspension of each strain and incubated at 26°C for 7 days. (B) Quantitative real-time PCR (qRT-PCR) expression profile of Bbgas genes (1,–6) in the B. bassiana ΔBbgas3 mutant as a function of the pH of the growth media. Fungal cultures were grown RNA extracted as detailed in Materials and Methods. Error bars are the SD of the mean of the results from three replicate assays; asterisks indicate significant differences (Tukey's HSD, P < 0.01).

    Article Snippet: B. bassiana wild-type strains Bb0062 and ATCC 90517 and various mutants were routinely grown/maintained on potato dextrose broth/agar (PDB/PDA) or Czapek-dox broth/agar (CZB/CZA) (BD Difco, Sparks, MD, USA) at 26°C for 7 to 14 days.

    Techniques: Mutagenesis, Incubation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing

    Effect of Bbgas3 deletion on B. bassiana cell wall components. (A) Total carbohydrate, alkaline-soluble and -insoluble, and chitin content of B. bassiana wild-type and ΔBbgas3 mutant cells grown in media buffered to pH 7 and pH 10, and analyzed as detailed in Materials and Methods. Error bars are the SD of the mean of the results from three replicate assays, and asterisks on column bars indicate significant difference (Tukey's HSD, P < 0.01). (B) Calcofluor white staining of wild-type and ΔBbgas3 mutant cells. Inset and arrow indicate enhanced chitin deposition in the hyphal tip of the mutant at pH. Scale bar = 500 μm.

    Journal: Applied and Environmental Microbiology

    Article Title: The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions

    doi: 10.1128/AEM.01086-18

    Figure Lengend Snippet: Effect of Bbgas3 deletion on B. bassiana cell wall components. (A) Total carbohydrate, alkaline-soluble and -insoluble, and chitin content of B. bassiana wild-type and ΔBbgas3 mutant cells grown in media buffered to pH 7 and pH 10, and analyzed as detailed in Materials and Methods. Error bars are the SD of the mean of the results from three replicate assays, and asterisks on column bars indicate significant difference (Tukey's HSD, P < 0.01). (B) Calcofluor white staining of wild-type and ΔBbgas3 mutant cells. Inset and arrow indicate enhanced chitin deposition in the hyphal tip of the mutant at pH. Scale bar = 500 μm.

    Article Snippet: B. bassiana wild-type strains Bb0062 and ATCC 90517 and various mutants were routinely grown/maintained on potato dextrose broth/agar (PDB/PDA) or Czapek-dox broth/agar (CZB/CZA) (BD Difco, Sparks, MD, USA) at 26°C for 7 to 14 days.

    Techniques: Mutagenesis, Staining

    Analysis of conidial cell walls and morphology of the B. bassiana wild type (WT) and ΔBbgas3 mutant. (A and B) TEM images and quantification of conidial cell wall thickness. Scale bar = 0.2 μm. (C and D) Representative images and quantification of distorted conidial morphologies seen in the ΔBbgas3 mutant. Arrows indicate measurement of cell wall thickness. Scale bar = 0.5 μm. Asterisks on column bars indicate significant difference (Tukey's HSD, P < 0.01).

    Journal: Applied and Environmental Microbiology

    Article Title: The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions

    doi: 10.1128/AEM.01086-18

    Figure Lengend Snippet: Analysis of conidial cell walls and morphology of the B. bassiana wild type (WT) and ΔBbgas3 mutant. (A and B) TEM images and quantification of conidial cell wall thickness. Scale bar = 0.2 μm. (C and D) Representative images and quantification of distorted conidial morphologies seen in the ΔBbgas3 mutant. Arrows indicate measurement of cell wall thickness. Scale bar = 0.5 μm. Asterisks on column bars indicate significant difference (Tukey's HSD, P < 0.01).

    Article Snippet: B. bassiana wild-type strains Bb0062 and ATCC 90517 and various mutants were routinely grown/maintained on potato dextrose broth/agar (PDB/PDA) or Czapek-dox broth/agar (CZB/CZA) (BD Difco, Sparks, MD, USA) at 26°C for 7 to 14 days.

    Techniques: Mutagenesis

    Insect bioassays. G. mellonella larvae were treated either topically (A, C, and E) or via intrahemocoel injection (B, D, and F) with conidia from wild-type B. bassiana (■), ΔBbgas3 mutant (●), ΔBbgas3::Bbgas3 complemented (◆) strains, or mock-treated controls (○), as described in Materials and Methods. (A to F) Conidia derived from growth on PDA at pH 5.6 (A and B). 7.0 (C and D), and pH 10 (E and F). The percent survival ± SD over the indicated time course is presented.

    Journal: Applied and Environmental Microbiology

    Article Title: The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions

    doi: 10.1128/AEM.01086-18

    Figure Lengend Snippet: Insect bioassays. G. mellonella larvae were treated either topically (A, C, and E) or via intrahemocoel injection (B, D, and F) with conidia from wild-type B. bassiana (■), ΔBbgas3 mutant (●), ΔBbgas3::Bbgas3 complemented (◆) strains, or mock-treated controls (○), as described in Materials and Methods. (A to F) Conidia derived from growth on PDA at pH 5.6 (A and B). 7.0 (C and D), and pH 10 (E and F). The percent survival ± SD over the indicated time course is presented.

    Article Snippet: B. bassiana wild-type strains Bb0062 and ATCC 90517 and various mutants were routinely grown/maintained on potato dextrose broth/agar (PDB/PDA) or Czapek-dox broth/agar (CZB/CZA) (BD Difco, Sparks, MD, USA) at 26°C for 7 to 14 days.

    Techniques: Injection, Mutagenesis, Derivative Assay

    Flow cytometry distribution and quantification of lectin and β-glucan antibody staining in B. bassiana wild-type (black) and ΔBbgas3 mutant (green) aerial conidia. Error bars are the SD of the mean of the results from three replicate assays. ConA and WGA are the lectins of wheat germ agglutinin and concanavalin A, respectively. Stars on column bars indicate significant differences (Tukey's HSD, P < 0.01).

    Journal: Applied and Environmental Microbiology

    Article Title: The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions

    doi: 10.1128/AEM.01086-18

    Figure Lengend Snippet: Flow cytometry distribution and quantification of lectin and β-glucan antibody staining in B. bassiana wild-type (black) and ΔBbgas3 mutant (green) aerial conidia. Error bars are the SD of the mean of the results from three replicate assays. ConA and WGA are the lectins of wheat germ agglutinin and concanavalin A, respectively. Stars on column bars indicate significant differences (Tukey's HSD, P < 0.01).

    Article Snippet: B. bassiana wild-type strains Bb0062 and ATCC 90517 and various mutants were routinely grown/maintained on potato dextrose broth/agar (PDB/PDA) or Czapek-dox broth/agar (CZB/CZA) (BD Difco, Sparks, MD, USA) at 26°C for 7 to 14 days.

    Techniques: Flow Cytometry, Staining, Mutagenesis

    (A) Growth of B. bassiana wild-type, ΔBbgas3 mutant, and complemented (ΔBbgas3::Bbgas3) strains on CZA containing 0.05% bromocresol purple indicating ambient acidification. (B) Determination of the pH of growth media (CZB) inoculated with each respective fungal strain and measured at the indicated time course. (C) Growth of the wild-type, ΔBbpacC mutant, and ΔBbpacC::Bbgas3 complemented strains are shown on buffered PDA at indicated starting pH values, as described in Materials and Methods. Plates were inoculated with conidial suspensions and incubated at 26°C for 7 days.

    Journal: Applied and Environmental Microbiology

    Article Title: The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions

    doi: 10.1128/AEM.01086-18

    Figure Lengend Snippet: (A) Growth of B. bassiana wild-type, ΔBbgas3 mutant, and complemented (ΔBbgas3::Bbgas3) strains on CZA containing 0.05% bromocresol purple indicating ambient acidification. (B) Determination of the pH of growth media (CZB) inoculated with each respective fungal strain and measured at the indicated time course. (C) Growth of the wild-type, ΔBbpacC mutant, and ΔBbpacC::Bbgas3 complemented strains are shown on buffered PDA at indicated starting pH values, as described in Materials and Methods. Plates were inoculated with conidial suspensions and incubated at 26°C for 7 days.

    Article Snippet: B. bassiana wild-type strains Bb0062 and ATCC 90517 and various mutants were routinely grown/maintained on potato dextrose broth/agar (PDB/PDA) or Czapek-dox broth/agar (CZB/CZA) (BD Difco, Sparks, MD, USA) at 26°C for 7 to 14 days.

    Techniques: Mutagenesis, Incubation