a vinelandii atcc 9046  (ATCC)

Journal:

Article Title: Azotobacter vinelandii Aldehyde Dehydrogenase Regulated by ? 54 : Role in Alcohol Catabolism and Encystment

doi: 10.1128/JB.183.21.6169-6174.2001

Figure Lengend Snippet: Bacterial strains and plasmids used in this work

Article Snippet: As expected, an increase in β-galactosidase activity was observed during incubation on n -butanol (Fig. ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Growth phenotypes of A. vinelandii ATCC 9046 (1), SG9A (2), SG9A::pSM9-Gm (3), and CN10 (4) at 30°C on Burk's medium supplemented with 10 mM NH 4 Cl as the nitrogen source and with 2.0% sucrose (A), 0.2% BHB (B), n -butanol (C), ethanol (D), or hexanol (E) as the sole carbon source. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3 caption a7 β-Galactosidase activity of strain SG9A, during incubation in solid Burk's medium with sucrose (open symbols) or n -butanol (closed symbols).

Techniques: Plasmid Preparation, Subcloning, Mutagenesis

Journal:

Article Title: Azotobacter vinelandii Aldehyde Dehydrogenase Regulated by ? 54 : Role in Alcohol Catabolism and Encystment

doi: 10.1128/JB.183.21.6169-6174.2001

Figure Lengend Snippet: Growth phenotypes of A. vinelandii ATCC 9046 (1), SG9A (2), SG9A::pSM9-Gm (3), and CN10 (4) at 30°C on Burk's medium supplemented with 10 mM NH4Cl as the nitrogen source and with 2.0% sucrose (A), 0.2% BHB (B), n-butanol (C), ethanol (D), or hexanol (E) as the sole carbon source.

Article Snippet: As expected, an increase in β-galactosidase activity was observed during incubation on n -butanol (Fig. ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Growth phenotypes of A. vinelandii ATCC 9046 (1), SG9A (2), SG9A::pSM9-Gm (3), and CN10 (4) at 30°C on Burk's medium supplemented with 10 mM NH 4 Cl as the nitrogen source and with 2.0% sucrose (A), 0.2% BHB (B), n -butanol (C), ethanol (D), or hexanol (E) as the sole carbon source. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3 caption a7 β-Galactosidase activity of strain SG9A, during incubation in solid Burk's medium with sucrose (open symbols) or n -butanol (closed symbols).

Techniques:

Journal:

Article Title: Azotobacter vinelandii Aldehyde Dehydrogenase Regulated by ? 54 : Role in Alcohol Catabolism and Encystment

doi: 10.1128/JB.183.21.6169-6174.2001

Figure Lengend Snippet: Aldehyde dehydrogenase activity in A. vinelandii aldA mutant a

Article Snippet: As expected, an increase in β-galactosidase activity was observed during incubation on n -butanol (Fig. ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Growth phenotypes of A. vinelandii ATCC 9046 (1), SG9A (2), SG9A::pSM9-Gm (3), and CN10 (4) at 30°C on Burk's medium supplemented with 10 mM NH 4 Cl as the nitrogen source and with 2.0% sucrose (A), 0.2% BHB (B), n -butanol (C), ethanol (D), or hexanol (E) as the sole carbon source. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3 caption a7 β-Galactosidase activity of strain SG9A, during incubation in solid Burk's medium with sucrose (open symbols) or n -butanol (closed symbols).

Techniques: Activity Assay, Mutagenesis

Journal:

Article Title: Azotobacter vinelandii Aldehyde Dehydrogenase Regulated by ? 54 : Role in Alcohol Catabolism and Encystment

doi: 10.1128/JB.183.21.6169-6174.2001

Figure Lengend Snippet: Primer extension analysis of aldA transcription in strains ATCC 9046 and CN10. (A) DNA sequence of the 5′ end of aldA. The arrow indicates the start site of aldA transcription; the ς54 promoter is also indicated at the top. The ATG initiation codon is shown in boldface. The complementary sequence where the oligonucleotide used for primer extension was generated is underlined. (B) Primer extension of the aldA gene in strain ATCC 9046 (lane 1) and in strain CN10 (lane 2) incubated in Burk's medium plus ammonia with sucrose. The aldA sequence ladder (GATC) was produced with the oligonucleotide used for primer extension.

Article Snippet: As expected, an increase in β-galactosidase activity was observed during incubation on n -butanol (Fig. ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Growth phenotypes of A. vinelandii ATCC 9046 (1), SG9A (2), SG9A::pSM9-Gm (3), and CN10 (4) at 30°C on Burk's medium supplemented with 10 mM NH 4 Cl as the nitrogen source and with 2.0% sucrose (A), 0.2% BHB (B), n -butanol (C), ethanol (D), or hexanol (E) as the sole carbon source. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3 caption a7 β-Galactosidase activity of strain SG9A, during incubation in solid Burk's medium with sucrose (open symbols) or n -butanol (closed symbols).

Techniques: Sequencing, Generated, Incubation, Produced

Journal:

Article Title: Azotobacter vinelandii Aldehyde Dehydrogenase Regulated by ? 54 : Role in Alcohol Catabolism and Encystment

doi: 10.1128/JB.183.21.6169-6174.2001

Figure Lengend Snippet: Encystment in A. vinelandii aldA and rpoN mutants

Article Snippet: As expected, an increase in β-galactosidase activity was observed during incubation on n -butanol (Fig. ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Growth phenotypes of A. vinelandii ATCC 9046 (1), SG9A (2), SG9A::pSM9-Gm (3), and CN10 (4) at 30°C on Burk's medium supplemented with 10 mM NH 4 Cl as the nitrogen source and with 2.0% sucrose (A), 0.2% BHB (B), n -butanol (C), ethanol (D), or hexanol (E) as the sole carbon source. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3 caption a7 β-Galactosidase activity of strain SG9A, during incubation in solid Burk's medium with sucrose (open symbols) or n -butanol (closed symbols).

Techniques: