s marcescens atcc  (ATCC)


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    ATCC s marcescens atcc
    Quorum sensing inhibitory potential of ADPE against S. <t>marcescens</t> . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.
    S Marcescens Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins"

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-16507-x

    Quorum sensing inhibitory potential of ADPE against S. marcescens . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.
    Figure Legend Snippet: Quorum sensing inhibitory potential of ADPE against S. marcescens . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.

    Techniques Used: Concentration Assay, Inhibition, Incubation

    Quorum sensing inhibitory potential of active lead vanillic acid. Effect of pure vanillic acid on the QS regulated biofilm formation and prodigiosin, protease, hemolysin and lipase production of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( a ). Light ( b ) and CLSM [Three dimensional micrographs] ( c ) analyses corroborate the antibiofilm activity of vanillic acid. Effect of vanillic acid on the swarming motility of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( d ). Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.
    Figure Legend Snippet: Quorum sensing inhibitory potential of active lead vanillic acid. Effect of pure vanillic acid on the QS regulated biofilm formation and prodigiosin, protease, hemolysin and lipase production of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( a ). Light ( b ) and CLSM [Three dimensional micrographs] ( c ) analyses corroborate the antibiofilm activity of vanillic acid. Effect of vanillic acid on the swarming motility of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( d ). Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.

    Techniques Used: Activity Assay

    Growth curve analysis depicting the non-antibacterial/bacteriostatic nature of S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) grown in the presence and absence of vanillic acid. Error bars represent standard deviations from the mean (n = 3 [biological triplicates]). FTIR analysis of EPS extracted from control and vanillic acid treated S. marcescens CI ( d ). Panel e, f and g depict the variations in the polysaccharide, nucleic acid, proteins and fatty acid region of EPS, respectively.
    Figure Legend Snippet: Growth curve analysis depicting the non-antibacterial/bacteriostatic nature of S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) grown in the presence and absence of vanillic acid. Error bars represent standard deviations from the mean (n = 3 [biological triplicates]). FTIR analysis of EPS extracted from control and vanillic acid treated S. marcescens CI ( d ). Panel e, f and g depict the variations in the polysaccharide, nucleic acid, proteins and fatty acid region of EPS, respectively.

    Techniques Used:

    Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d & e ) and intestinal colonization ( d , e & f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h & i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p < 0.05 and p < 0.01, respectively.
    Figure Legend Snippet: Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d & e ) and intestinal colonization ( d , e & f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h & i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p < 0.05 and p < 0.01, respectively.

    Techniques Used: Infection, Inhibition, Colony-forming Unit Assay

    Representative gel pictures depicting the analysis of intracellular protein extract of S. marcescens CI grown in the absence and presence of 250 µg/mL of vanillic acid using 2-D gel electrophoresis. Each 450 µg of protein extract from control and treated cells were subjected to isoelectric focusing and resolved based on molecular weight in 10–15% gradient SDS-PAGE and protein spots were stained with MS compatible colloidal CBB. Up regulated and down regulated spots are encircled with green and red, respectively.
    Figure Legend Snippet: Representative gel pictures depicting the analysis of intracellular protein extract of S. marcescens CI grown in the absence and presence of 250 µg/mL of vanillic acid using 2-D gel electrophoresis. Each 450 µg of protein extract from control and treated cells were subjected to isoelectric focusing and resolved based on molecular weight in 10–15% gradient SDS-PAGE and protein spots were stained with MS compatible colloidal CBB. Up regulated and down regulated spots are encircled with green and red, respectively.

    Techniques Used: Nucleic Acid Electrophoresis, Molecular Weight, SDS Page, Staining

    List of differentially expressed proteins of S. marcescens upon vanillic acid treatment identified using nano-LC-MS/MS and MALDI-TOF/TOF.
    Figure Legend Snippet: List of differentially expressed proteins of S. marcescens upon vanillic acid treatment identified using nano-LC-MS/MS and MALDI-TOF/TOF.

    Techniques Used:

    Gene ontology analysis of down and upregulated proteins of S . marcescens upon vanillic acid treatment.
    Figure Legend Snippet: Gene ontology analysis of down and upregulated proteins of S . marcescens upon vanillic acid treatment.

    Techniques Used:

    MR-VP test results depicting the effect of vanillic acid treatment on acid ( a ) and butanediol ( b ) production in S. marcescens CI. Effect of different concentration of vanillic acid treatment on the CSH ( c ) and the growth of S. marcescens CI in the presence of 0.5 and 1 M NaCl ( d ). Error bars represent standard deviations from the mean (n = 3). *(p < 0.05) indicates the statistical value of vanillic acid treated compared to control. Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b and c indicate the significant difference p < 0.05, p < 0.01 and p < 0.005, respectively.
    Figure Legend Snippet: MR-VP test results depicting the effect of vanillic acid treatment on acid ( a ) and butanediol ( b ) production in S. marcescens CI. Effect of different concentration of vanillic acid treatment on the CSH ( c ) and the growth of S. marcescens CI in the presence of 0.5 and 1 M NaCl ( d ). Error bars represent standard deviations from the mean (n = 3). *(p < 0.05) indicates the statistical value of vanillic acid treated compared to control. Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b and c indicate the significant difference p < 0.05, p < 0.01 and p < 0.005, respectively.

    Techniques Used: Concentration Assay

    s marcescens atcc  (ATCC)


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    ATCC s marcescens atcc
    Quorum sensing inhibitory potential of ADPE against S. <t>marcescens</t> . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.
    S Marcescens Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s marcescens atcc/product/ATCC
    Average 94 stars, based on 1 article reviews
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    s marcescens atcc - by Bioz Stars, 2023-04
    94/100 stars

    Images

    1) Product Images from "Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins"

    Article Title: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-16507-x

    Quorum sensing inhibitory potential of ADPE against S. marcescens . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.
    Figure Legend Snippet: Quorum sensing inhibitory potential of ADPE against S. marcescens . The ADPE inhibited the QS regulated virulence factors such as biofilm formation, prodigiosin, protease, hemolysin and lipase ( a ) in a dose dependent manner without inhibiting the growth of S. marcescens ( b ). Light microscopic visualization of S. marcescens biofilm formed in the absence and presence of ADPE ( c ). Effect of solvent extracts of ADPEE against prodigiosin production and the growth ( d ) of S . marcescens . ADPEE-C exhibited the concentration dependent inhibition of biofilm formation, protease and lipase ( e ) without affecting the growth ( f ). Growth of S. marcescens was measured at 600 nm after 18 h incubation at 30 °C. Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.

    Techniques Used: Concentration Assay, Inhibition, Incubation

    Quorum sensing inhibitory potential of active lead vanillic acid. Effect of pure vanillic acid on the QS regulated biofilm formation and prodigiosin, protease, hemolysin and lipase production of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( a ). Light ( b ) and CLSM [Three dimensional micrographs] ( c ) analyses corroborate the antibiofilm activity of vanillic acid. Effect of vanillic acid on the swarming motility of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( d ). Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.
    Figure Legend Snippet: Quorum sensing inhibitory potential of active lead vanillic acid. Effect of pure vanillic acid on the QS regulated biofilm formation and prodigiosin, protease, hemolysin and lipase production of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( a ). Light ( b ) and CLSM [Three dimensional micrographs] ( c ) analyses corroborate the antibiofilm activity of vanillic acid. Effect of vanillic acid on the swarming motility of S. marcescens CI, S. marcescens ATCC and S. marcescens MG1 ( d ). Error bars represent standard deviations from the mean (n = 6 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b, c and d indicate the significant difference p < 0.05, p < 0.01, p < 0.005 and p < 0.001, respectively.

    Techniques Used: Activity Assay

    Growth curve analysis depicting the non-antibacterial/bacteriostatic nature of S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) grown in the presence and absence of vanillic acid. Error bars represent standard deviations from the mean (n = 3 [biological triplicates]). FTIR analysis of EPS extracted from control and vanillic acid treated S. marcescens CI ( d ). Panel e, f and g depict the variations in the polysaccharide, nucleic acid, proteins and fatty acid region of EPS, respectively.
    Figure Legend Snippet: Growth curve analysis depicting the non-antibacterial/bacteriostatic nature of S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) grown in the presence and absence of vanillic acid. Error bars represent standard deviations from the mean (n = 3 [biological triplicates]). FTIR analysis of EPS extracted from control and vanillic acid treated S. marcescens CI ( d ). Panel e, f and g depict the variations in the polysaccharide, nucleic acid, proteins and fatty acid region of EPS, respectively.

    Techniques Used:

    Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d & e ) and intestinal colonization ( d , e & f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h & i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p < 0.05 and p < 0.01, respectively.
    Figure Legend Snippet: Survival graph showing the ability of vanillic acid treatment to rescue C. elegans from S. marcescens CI ( a ), S. marcescens ATCC ( b ) and S. marcescens MG1 ( c ) infection. Light micrographs depict the inhibition of prodigiosin pigment ( d & e ) and intestinal colonization ( d , e & f ) of S. marcescens upon vanillic acid treatment when compared to control. CFU assay results further confirm the inhibition of intestinal colonization of S. marcescens strains ( g , h & i ). Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a and b indicate the significant difference p < 0.05 and p < 0.01, respectively.

    Techniques Used: Infection, Inhibition, Colony-forming Unit Assay

    Representative gel pictures depicting the analysis of intracellular protein extract of S. marcescens CI grown in the absence and presence of 250 µg/mL of vanillic acid using 2-D gel electrophoresis. Each 450 µg of protein extract from control and treated cells were subjected to isoelectric focusing and resolved based on molecular weight in 10–15% gradient SDS-PAGE and protein spots were stained with MS compatible colloidal CBB. Up regulated and down regulated spots are encircled with green and red, respectively.
    Figure Legend Snippet: Representative gel pictures depicting the analysis of intracellular protein extract of S. marcescens CI grown in the absence and presence of 250 µg/mL of vanillic acid using 2-D gel electrophoresis. Each 450 µg of protein extract from control and treated cells were subjected to isoelectric focusing and resolved based on molecular weight in 10–15% gradient SDS-PAGE and protein spots were stained with MS compatible colloidal CBB. Up regulated and down regulated spots are encircled with green and red, respectively.

    Techniques Used: Nucleic Acid Electrophoresis, Molecular Weight, SDS Page, Staining

    List of differentially expressed proteins of S. marcescens upon vanillic acid treatment identified using nano-LC-MS/MS and MALDI-TOF/TOF.
    Figure Legend Snippet: List of differentially expressed proteins of S. marcescens upon vanillic acid treatment identified using nano-LC-MS/MS and MALDI-TOF/TOF.

    Techniques Used:

    Gene ontology analysis of down and upregulated proteins of S . marcescens upon vanillic acid treatment.
    Figure Legend Snippet: Gene ontology analysis of down and upregulated proteins of S . marcescens upon vanillic acid treatment.

    Techniques Used:

    MR-VP test results depicting the effect of vanillic acid treatment on acid ( a ) and butanediol ( b ) production in S. marcescens CI. Effect of different concentration of vanillic acid treatment on the CSH ( c ) and the growth of S. marcescens CI in the presence of 0.5 and 1 M NaCl ( d ). Error bars represent standard deviations from the mean (n = 3). *(p < 0.05) indicates the statistical value of vanillic acid treated compared to control. Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b and c indicate the significant difference p < 0.05, p < 0.01 and p < 0.005, respectively.
    Figure Legend Snippet: MR-VP test results depicting the effect of vanillic acid treatment on acid ( a ) and butanediol ( b ) production in S. marcescens CI. Effect of different concentration of vanillic acid treatment on the CSH ( c ) and the growth of S. marcescens CI in the presence of 0.5 and 1 M NaCl ( d ). Error bars represent standard deviations from the mean (n = 3). *(p < 0.05) indicates the statistical value of vanillic acid treated compared to control. Error bars represent standard deviations from the mean (n = 3 [biological triplicates in experimental duplicates]). Statistical significance was analyzed using one way ANOVA-Duncan’s post-hoc test. a, b and c indicate the significant difference p < 0.05, p < 0.01 and p < 0.005, respectively.

    Techniques Used: Concentration Assay

    mgas5005 138•21•90•57•138•96•102•191•304  (ATCC)


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    ATCC mgas5005 138•21•90•57•138•96•102•191•304
    Mgas5005 138•21•90•57•138•96•102•191•304, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    104 cell lines 105 u937  (ATCC)


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    ATCC 104 cell lines 105 u937
    104 Cell Lines 105 U937, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    106 peerj preprints  (ATCC)


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    ATCC 106 peerj preprints
    106 Peerj Preprints, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    102 cell line  (ATCC)


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    ATCC 102 cell line
    102 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 extracts  (ATCC)


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    ATCC 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 extracts
    102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 Extracts, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 extracts - by Bioz Stars, 2023-04
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    s cerevisiae no 102 ypd medium 1 95 aa 3 90 b 10 10  (ATCC)


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    ATCC s cerevisiae no 102 ypd medium 1 95 aa 3 90 b 10 10
    S Cerevisiae No 102 Ypd Medium 1 95 Aa 3 90 B 10 10, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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