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JEOL 90 nm ultrathin sections
90 Nm Ultrathin Sections, supplied by JEOL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/90 nm ultrathin sections/product/JEOL
Average 93 stars, based on 1 article reviews
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90 nm ultrathin sections - by Bioz Stars, 2020-08
93/100 stars

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Electron Microscopy:

Article Title: The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer
Article Snippet: .. For transmission electron microscopy (TEM), 90-nm ultrathin sections (JEOL JUM-7 ultramicrotome) were placed on grids which were later post-stained with uranyl acetate/lead citrate, examined using a Zeiss Leo 906-E electron microscope (Oberkochen, Germany), and photographed with a Megaview III camera (Olympus, Center Valley, PA). ..

Transmission Electron Microscopy:

Article Title: The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer
Article Snippet: .. For transmission electron microscopy (TEM), 90-nm ultrathin sections (JEOL JUM-7 ultramicrotome) were placed on grids which were later post-stained with uranyl acetate/lead citrate, examined using a Zeiss Leo 906-E electron microscope (Oberkochen, Germany), and photographed with a Megaview III camera (Olympus, Center Valley, PA). ..

Microscopy:

Article Title: The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer
Article Snippet: .. For transmission electron microscopy (TEM), 90-nm ultrathin sections (JEOL JUM-7 ultramicrotome) were placed on grids which were later post-stained with uranyl acetate/lead citrate, examined using a Zeiss Leo 906-E electron microscope (Oberkochen, Germany), and photographed with a Megaview III camera (Olympus, Center Valley, PA). ..

Transmission Assay:

Article Title: The Fat Body of the Hematophagous Insect, Panstrongylus megistus (Hemiptera: Reduviidae): Histological Features and Participation of the β-Chain of ATP Synthase in the Lipophorin-Mediated Lipid Transfer
Article Snippet: .. For transmission electron microscopy (TEM), 90-nm ultrathin sections (JEOL JUM-7 ultramicrotome) were placed on grids which were later post-stained with uranyl acetate/lead citrate, examined using a Zeiss Leo 906-E electron microscope (Oberkochen, Germany), and photographed with a Megaview III camera (Olympus, Center Valley, PA). ..

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    JEOL ultrathin sections
    Rescue by genomic rnRacGAP transgene of membrane and mitochondrial defects in rn null mutants. (A, D, G) Wild type. (B, E, H) Null mutant rn 20 red p p / rn 20 red . (C, F, I) Transgenic fly P(rnRacGAP) 13 rn 20 /rn 20 p p . (A, B, C) Optical microscopy of semithin sections of dissected reproductive organs stained with toluidine blue. White arrows, early-stage spermatocytes; black arrows, mature cysts in longitudinal section, with long, darkly staining flagella; black arrowheads, hexagonally shaped cysts in transverse section; white arrowhead, section of seminal vesicle; black-and-white arrowheads, abnormal vacuolated cysts. Bar, 10 μm. (D to I) Transmission electron microscopy of <t>ultrathin</t> sections of testes. (D to F) In comparison to wild-type cysts, rn null cysts are disorganized and developing spermatids are randomly oriented. The rnRacGAP transgene restores spermatid orientation, resulting in a more orderly distribution. Bar, 1.2 μm. (G to I) Magnified views of mature sperm from panels D to F, respectively. Bar, 230 nm. The white arrow in panel H points to a fully retracted minor mitochondrial derivative.
    Ultrathin Sections, supplied by JEOL, used in various techniques. Bioz Stars score: 95/100, based on 3004 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrathin sections/product/JEOL
    Average 95 stars, based on 3004 article reviews
    Price from $9.99 to $1999.99
    ultrathin sections - by Bioz Stars, 2020-08
    95/100 stars
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    Rescue by genomic rnRacGAP transgene of membrane and mitochondrial defects in rn null mutants. (A, D, G) Wild type. (B, E, H) Null mutant rn 20 red p p / rn 20 red . (C, F, I) Transgenic fly P(rnRacGAP) 13 rn 20 /rn 20 p p . (A, B, C) Optical microscopy of semithin sections of dissected reproductive organs stained with toluidine blue. White arrows, early-stage spermatocytes; black arrows, mature cysts in longitudinal section, with long, darkly staining flagella; black arrowheads, hexagonally shaped cysts in transverse section; white arrowhead, section of seminal vesicle; black-and-white arrowheads, abnormal vacuolated cysts. Bar, 10 μm. (D to I) Transmission electron microscopy of ultrathin sections of testes. (D to F) In comparison to wild-type cysts, rn null cysts are disorganized and developing spermatids are randomly oriented. The rnRacGAP transgene restores spermatid orientation, resulting in a more orderly distribution. Bar, 1.2 μm. (G to I) Magnified views of mature sperm from panels D to F, respectively. Bar, 230 nm. The white arrow in panel H points to a fully retracted minor mitochondrial derivative.

    Journal: Molecular and Cellular Biology

    Article Title: RotundRacGAP Functions with Ras during Spermatogenesis and Retinal Differentiation in Drosophila melanogaster

    doi: 10.1128/MCB.21.18.6280-6291.2001

    Figure Lengend Snippet: Rescue by genomic rnRacGAP transgene of membrane and mitochondrial defects in rn null mutants. (A, D, G) Wild type. (B, E, H) Null mutant rn 20 red p p / rn 20 red . (C, F, I) Transgenic fly P(rnRacGAP) 13 rn 20 /rn 20 p p . (A, B, C) Optical microscopy of semithin sections of dissected reproductive organs stained with toluidine blue. White arrows, early-stage spermatocytes; black arrows, mature cysts in longitudinal section, with long, darkly staining flagella; black arrowheads, hexagonally shaped cysts in transverse section; white arrowhead, section of seminal vesicle; black-and-white arrowheads, abnormal vacuolated cysts. Bar, 10 μm. (D to I) Transmission electron microscopy of ultrathin sections of testes. (D to F) In comparison to wild-type cysts, rn null cysts are disorganized and developing spermatids are randomly oriented. The rnRacGAP transgene restores spermatid orientation, resulting in a more orderly distribution. Bar, 1.2 μm. (G to I) Magnified views of mature sperm from panels D to F, respectively. Bar, 230 nm. The white arrow in panel H points to a fully retracted minor mitochondrial derivative.

    Article Snippet: Thin sections (2 μm) stained with toluidine blue were examined by phase-contrast and fluorescence optical microscopy; ultrathin sections (90 nm) stained with heavy metal were examined with a JEOL 1200ExII electron microscope.

    Techniques: Mutagenesis, Transgenic Assay, Microscopy, Staining, Transmission Assay, Electron Microscopy

    Electron micrographs of liver ultrathin section of Group IV showing (a) Multiple hepatocytes some of them are binucleated with prominent nucleolus (Nu), (b) Preserved ultrastructural picture including mitochondria (M) and rough endoplasmic reticulum (R). (c) Others showing many lipid droplets (L). (d) Rarefied cytoplasm (*). (e) Nuclei with condensed chromatin (N) and (f) Bundles of collagen fibers (arrow) in between the hepatocytes

    Journal: Journal of Microscopy and Ultrastructure

    Article Title: Histological, Immunohistochemical, and Biochemical Study of Experimentally Induced Fatty Liver in Adult Male Albino Rat and the Possible Protective Role of Pomegranate

    doi: 10.4103/JMAU.JMAU_5_18

    Figure Lengend Snippet: Electron micrographs of liver ultrathin section of Group IV showing (a) Multiple hepatocytes some of them are binucleated with prominent nucleolus (Nu), (b) Preserved ultrastructural picture including mitochondria (M) and rough endoplasmic reticulum (R). (c) Others showing many lipid droplets (L). (d) Rarefied cytoplasm (*). (e) Nuclei with condensed chromatin (N) and (f) Bundles of collagen fibers (arrow) in between the hepatocytes

    Article Snippet: Semithin sections (1 mm thick) were stained with 1% toluidine blue and examined by light microscope for proper orientation while ultrathin sections (80–90 nm (were stained with uranyl acetate and lead citrate[ ] to be examined by JEOL-JEM-100 transmission electron microscope) Tokyo, Japan) at the Electron Microscopic Unit, Faculty of Medicine, Tanta University.

    Techniques:

    Electron micrographs of liver ultrathin sections of control group showing (a) Polyhedral-shaped hepatocyte-containing large rounded nucleus (N) with extended chromatin and prominent nucleolus. (b) The cytoplasm-containing abundant mitochondria (M), rER (R), glycogen granules (G), and multiple peroxisomes (arrowhead). (c) Space of Disse between hepatocytes (H) and blood sinusoids (S) containing HSCs cell (arrow) with multiple lipid with droplets (L). (d) Kupffer cell (double arrow) appears as irregular shaped cell with indented nucleus and multiple cytoplasmic dense bodies

    Journal: Journal of Microscopy and Ultrastructure

    Article Title: Histological, Immunohistochemical, and Biochemical Study of Experimentally Induced Fatty Liver in Adult Male Albino Rat and the Possible Protective Role of Pomegranate

    doi: 10.4103/JMAU.JMAU_5_18

    Figure Lengend Snippet: Electron micrographs of liver ultrathin sections of control group showing (a) Polyhedral-shaped hepatocyte-containing large rounded nucleus (N) with extended chromatin and prominent nucleolus. (b) The cytoplasm-containing abundant mitochondria (M), rER (R), glycogen granules (G), and multiple peroxisomes (arrowhead). (c) Space of Disse between hepatocytes (H) and blood sinusoids (S) containing HSCs cell (arrow) with multiple lipid with droplets (L). (d) Kupffer cell (double arrow) appears as irregular shaped cell with indented nucleus and multiple cytoplasmic dense bodies

    Article Snippet: Semithin sections (1 mm thick) were stained with 1% toluidine blue and examined by light microscope for proper orientation while ultrathin sections (80–90 nm (were stained with uranyl acetate and lead citrate[ ] to be examined by JEOL-JEM-100 transmission electron microscope) Tokyo, Japan) at the Electron Microscopic Unit, Faculty of Medicine, Tanta University.

    Techniques:

    Electron micrographs of liver ultrathin section of Group III showing many lipid droplets with variable size and shape (L) within the cytoplasm of the hepatocytes. (a and b) dilatation of rER (R).(c) Rarefaction of cytoplasm (*) and degenerative changes of mitochondria (M). (a and b) Nuclei of many hepatocytes showing condensed chromatin (N), irregularity, indentation (arrowhead) with widening of the perinuclear space (biffed arrow). (d and e) Many HSCs (arrow), bundles of collagen fibers (C). (f) Inflammatory cells (double arrow) present between the hepatocytes

    Journal: Journal of Microscopy and Ultrastructure

    Article Title: Histological, Immunohistochemical, and Biochemical Study of Experimentally Induced Fatty Liver in Adult Male Albino Rat and the Possible Protective Role of Pomegranate

    doi: 10.4103/JMAU.JMAU_5_18

    Figure Lengend Snippet: Electron micrographs of liver ultrathin section of Group III showing many lipid droplets with variable size and shape (L) within the cytoplasm of the hepatocytes. (a and b) dilatation of rER (R).(c) Rarefaction of cytoplasm (*) and degenerative changes of mitochondria (M). (a and b) Nuclei of many hepatocytes showing condensed chromatin (N), irregularity, indentation (arrowhead) with widening of the perinuclear space (biffed arrow). (d and e) Many HSCs (arrow), bundles of collagen fibers (C). (f) Inflammatory cells (double arrow) present between the hepatocytes

    Article Snippet: Semithin sections (1 mm thick) were stained with 1% toluidine blue and examined by light microscope for proper orientation while ultrathin sections (80–90 nm (were stained with uranyl acetate and lead citrate[ ] to be examined by JEOL-JEM-100 transmission electron microscope) Tokyo, Japan) at the Electron Microscopic Unit, Faculty of Medicine, Tanta University.

    Techniques:

    Presynaptic localization of ANO1 in the retina. Electron micrographs taken from vertical ultrathin sections (90 nm in thickness) of the mouse retina processed for ANO1 immunostaining. ( A, B ) Outer plexiform layer. In A , ANO1 immunoreactivity is localized to a cone pedicle (CP). At each ribbon synapse (arrowheads) within the CP, 2 horizontal dendrites (H) and an invaginating dendrite of ON-cone bipolar cell (I) are unlabeled. The basal dendrites of OFF-cone bipolar cells (B) at the CP base are also unlabeled. In B , a rod spherule (RS) shows ANO1 immunoreactivity. Similar to A , the postsynaptic triad comprising 2 horizontal dendrites (H) and an invaginating rod bipolar dendrite (I) do not exhibit ANO1 immunoreactivity. ( C, D ) Inner plexiform layer. In right side of C , a labeled cone bipolar terminal (BC+) establishes a ribbon synapse (arrowhead) onto a postsynaptic dyad composed of an unlabeled ganglion dendrite (G) and an unlabeled amarcrine process (A−). In addition, an unlabeled bipolar terminal (BC−) synapsing onto 2 unlabeled amacirne dendrites (A−) is seen in the left side. In D, a labeled amacrine process (A+) establishes a conventional chemical synapse onto an unlabeled amacrine process (A−). Scale bars, 0.5 µm.

    Journal: PLoS ONE

    Article Title: Presynaptic Localization and Possible Function of Calcium-Activated Chloride Channel Anoctamin 1 in the Mammalian Retina

    doi: 10.1371/journal.pone.0067989

    Figure Lengend Snippet: Presynaptic localization of ANO1 in the retina. Electron micrographs taken from vertical ultrathin sections (90 nm in thickness) of the mouse retina processed for ANO1 immunostaining. ( A, B ) Outer plexiform layer. In A , ANO1 immunoreactivity is localized to a cone pedicle (CP). At each ribbon synapse (arrowheads) within the CP, 2 horizontal dendrites (H) and an invaginating dendrite of ON-cone bipolar cell (I) are unlabeled. The basal dendrites of OFF-cone bipolar cells (B) at the CP base are also unlabeled. In B , a rod spherule (RS) shows ANO1 immunoreactivity. Similar to A , the postsynaptic triad comprising 2 horizontal dendrites (H) and an invaginating rod bipolar dendrite (I) do not exhibit ANO1 immunoreactivity. ( C, D ) Inner plexiform layer. In right side of C , a labeled cone bipolar terminal (BC+) establishes a ribbon synapse (arrowhead) onto a postsynaptic dyad composed of an unlabeled ganglion dendrite (G) and an unlabeled amarcrine process (A−). In addition, an unlabeled bipolar terminal (BC−) synapsing onto 2 unlabeled amacirne dendrites (A−) is seen in the left side. In D, a labeled amacrine process (A+) establishes a conventional chemical synapse onto an unlabeled amacrine process (A−). Scale bars, 0.5 µm.

    Article Snippet: Serial ultrathin sections (70–90 nm in thickness) were collected on single slot grids, and examined using a JEM 1010 electron microscope (JEOL, Tokyo, Japan).

    Techniques: Immunostaining, Labeling

    Three-dimensional (3D) images of PDGFR-β-positive cells in the lesion core at day 14 after 3-NP injection. (A–D) 3D reconstruction (D) of one PDGFR-β-positive cell is prepared from a series of 27 serial ultrathin sections (90-nm-thick), one of which is shown in (A–C) . A PDGFR-β-positive cell has a soma located at a distance from the vasculature and extends highly branched cytoplasmic processes, which frequently have close apposition with microglia/macrophages with amoeboid morphology, with little to no intervening space. (E–G) Another example of 3D reconstruction (G) of three PDGFR-β-positive cells, prepared from a series of 26 consecutive sections, one of which is shown in (E,F) . The three cells extend highly branched cytoplasmic processes that are frequently in close apposition or even interwoven with each other, forming a network. (B,C,F) Higher magnification images of the boxed areas in (A,E) , respectively. PDGFR-β-positive cells have focal cytoplasmic enlargements (arrows in B,C,E ; # in D,G ), from which distal branches arise. Note that their somata and processes are closely associated with collagen fibrils (asterisks in B,E ). The arrowhead in (E) denotes a nucleolus. Scale bars = 2 μm for (A,E) ; 1 μm for (B,C,F) .

    Journal: Frontiers in Molecular Neuroscience

    Article Title: PDGFR-β-Positive Perivascular Adventitial Cells Expressing Nestin Contribute to Fibrotic Scar Formation in the Striatum of 3-NP Intoxicated Rats

    doi: 10.3389/fnmol.2018.00402

    Figure Lengend Snippet: Three-dimensional (3D) images of PDGFR-β-positive cells in the lesion core at day 14 after 3-NP injection. (A–D) 3D reconstruction (D) of one PDGFR-β-positive cell is prepared from a series of 27 serial ultrathin sections (90-nm-thick), one of which is shown in (A–C) . A PDGFR-β-positive cell has a soma located at a distance from the vasculature and extends highly branched cytoplasmic processes, which frequently have close apposition with microglia/macrophages with amoeboid morphology, with little to no intervening space. (E–G) Another example of 3D reconstruction (G) of three PDGFR-β-positive cells, prepared from a series of 26 consecutive sections, one of which is shown in (E,F) . The three cells extend highly branched cytoplasmic processes that are frequently in close apposition or even interwoven with each other, forming a network. (B,C,F) Higher magnification images of the boxed areas in (A,E) , respectively. PDGFR-β-positive cells have focal cytoplasmic enlargements (arrows in B,C,E ; # in D,G ), from which distal branches arise. Note that their somata and processes are closely associated with collagen fibrils (asterisks in B,E ). The arrowhead in (E) denotes a nucleolus. Scale bars = 2 μm for (A,E) ; 1 μm for (B,C,F) .

    Article Snippet: After being cut into ultrathin sections (70–90-nm-thick), they were observed under an electron microscope (JEM 1010, JEOL, Tokyo, Japan) with slight uranyl acetate staining.

    Techniques: Injection