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Agilent technologies 8453 diode array spectrophotometer
Small acceptance angle spectra comparing purified red cells and hemoglobin in solution acquired from an Agilent <t>8453</t> spectrophotometer with an acceptance angle of 2°. The RBCs were purified by washing whole blood by centrifugation and passing the suspension through a leuko-reduction filter. The RBC concentration is approximately 4000 cells/μl. The spectrum of the hemoglobin solution represents a concentration of ~0.12 mg/ml.
8453 Diode Array Spectrophotometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/8453 diode array spectrophotometer/product/Agilent technologies
Average 99 stars, based on 36 article reviews
Price from $9.99 to $1999.99
8453 diode array spectrophotometer - by Bioz Stars, 2020-10
99/100 stars

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1) Product Images from "Hypochromicity in red blood cells: an experimental and theoretical investigation"

Article Title: Hypochromicity in red blood cells: an experimental and theoretical investigation

Journal: Biomedical Optics Express

doi: 10.1364/BOE.2.002126

Small acceptance angle spectra comparing purified red cells and hemoglobin in solution acquired from an Agilent 8453 spectrophotometer with an acceptance angle of 2°. The RBCs were purified by washing whole blood by centrifugation and passing the suspension through a leuko-reduction filter. The RBC concentration is approximately 4000 cells/μl. The spectrum of the hemoglobin solution represents a concentration of ~0.12 mg/ml.
Figure Legend Snippet: Small acceptance angle spectra comparing purified red cells and hemoglobin in solution acquired from an Agilent 8453 spectrophotometer with an acceptance angle of 2°. The RBCs were purified by washing whole blood by centrifugation and passing the suspension through a leuko-reduction filter. The RBC concentration is approximately 4000 cells/μl. The spectrum of the hemoglobin solution represents a concentration of ~0.12 mg/ml.

Techniques Used: Purification, Spectrophotometry, Centrifugation, Concentration Assay

2) Product Images from "Hypochromicity in red blood cells: an experimental and theoretical investigation"

Article Title: Hypochromicity in red blood cells: an experimental and theoretical investigation

Journal: Biomedical Optics Express

doi: 10.1364/BOE.2.002126

Small acceptance angle spectra comparing purified red cells and hemoglobin in solution acquired from an Agilent 8453 spectrophotometer with an acceptance angle of 2°. The RBCs were purified by washing whole blood by centrifugation and passing the suspension through a leuko-reduction filter. The RBC concentration is approximately 4000 cells/μl. The spectrum of the hemoglobin solution represents a concentration of ~0.12 mg/ml.
Figure Legend Snippet: Small acceptance angle spectra comparing purified red cells and hemoglobin in solution acquired from an Agilent 8453 spectrophotometer with an acceptance angle of 2°. The RBCs were purified by washing whole blood by centrifugation and passing the suspension through a leuko-reduction filter. The RBC concentration is approximately 4000 cells/μl. The spectrum of the hemoglobin solution represents a concentration of ~0.12 mg/ml.

Techniques Used: Purification, Spectrophotometry, Centrifugation, Concentration Assay

Related Articles

Concentration Assay:

Article Title: Gene cluster conservation identifies melanin and perylenequinone biosynthesis pathways in multiple plant pathogenic fungi
Article Snippet: .. Cercosporin concentration was calculated by measuring absorbance at 255 nm using an Agilent Cary 8454 UV–Visible spectrophotometer (Agilent Technologies, Inc., Santa Clara) and 21, 500 as the molar extinction coefficient (Milat and Blein, ). ..

Article Title: Ferric Heme as a CO/NO Sensor in the Nuclear Receptor Reverbβ by Coupling Gas binding to Electron Transfer
Article Snippet: .. All absorption measurements were carried out on an Agilent Cary 8454 UV-visible spectrophotometer inside the anaerobic chamber with less than 0.3 ppm dioxygen except for Bradford assays and heme concentration measurements, which were performed aerobically on a Shimadzu UV-2600 spectrophotometer. .. The Rev-DR2 DNA sequence was obtained from Integrated DNA Technologies (Coralville, IA).

In Vitro:

Article Title: Design of Biologically Active Binary Protein 2D Materials
Article Snippet: .. Kinetic optical characterization of in vitro assembliesArrays formation kinetics was determined by turbidity due to light scattering, monitored by absorption at 330 nm wavelength, using an Agilent Technologies (Santa Clara, CA) Cary 8454 UV-Vis spectrophotometer. ..

Spectrophotometry:

Article Title: Nanoscale Zinc Oxide Particles for Improving the Physiological and Sanitary Quality of a Mexican Landrace of Red Maize
Article Snippet: .. UV–Vis Spectral analysis was done using a Cary 8454 UV–Vis Diode Array System spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). ..

Article Title: Diverse protein assembly driven by metal and chelating amino acids with selectivity and tunability
Article Snippet: .. Protein concentration was determined by measuring the absorbance at 280 nm (UV–vis spectrophotometer, Agilent Technologies Cary 8454 UV–vis) by using the epsilon values of protein and bipyridine at 280 nm . ..

Article Title: 3-Hydroxykynurenine bound to eye lens proteins induces oxidative modifications in crystalline proteins through a type I photosensitizing mechanism.
Article Snippet: .. Photosensitized reactions mediated by endogenous chromophores have been associated with the etiology of age-related cataract disease. .. Photosensitized reactions mediated by endogenous chromophores have been associated with the etiology of age-related cataract disease.

Article Title: Gene cluster conservation identifies melanin and perylenequinone biosynthesis pathways in multiple plant pathogenic fungi
Article Snippet: .. Cercosporin concentration was calculated by measuring absorbance at 255 nm using an Agilent Cary 8454 UV–Visible spectrophotometer (Agilent Technologies, Inc., Santa Clara) and 21, 500 as the molar extinction coefficient (Milat and Blein, ). ..

Article Title: Ferric Heme as a CO/NO Sensor in the Nuclear Receptor Reverbβ by Coupling Gas binding to Electron Transfer
Article Snippet: .. All absorption measurements were carried out on an Agilent Cary 8454 UV-visible spectrophotometer inside the anaerobic chamber with less than 0.3 ppm dioxygen except for Bradford assays and heme concentration measurements, which were performed aerobically on a Shimadzu UV-2600 spectrophotometer. .. The Rev-DR2 DNA sequence was obtained from Integrated DNA Technologies (Coralville, IA).

Article Title: Design of Biologically Active Binary Protein 2D Materials
Article Snippet: .. Kinetic optical characterization of in vitro assembliesArrays formation kinetics was determined by turbidity due to light scattering, monitored by absorption at 330 nm wavelength, using an Agilent Technologies (Santa Clara, CA) Cary 8454 UV-Vis spectrophotometer. ..

Article Title: Superoxide is the critical driver of DOPAL autoxidation, lysyl adduct formation, and crosslinking of α-synuclein
Article Snippet: .. Absorbance at 400 nm was monitored on a Cary 8454 UV-Vis spectrophotometer (Agilent) with the sample temperature maintained at 37 °C in a Peltier cell. ..

Article Title: Relationship between Antioxidant and Anticancer Activity of Trihydroxyflavones
Article Snippet: .. Light absorbance was measured at a wavelength of 515 nm using a spectrophotometer: Cary 8454 UV-Vis (Agilent Technologies). ..

Protein Concentration:

Article Title: Diverse protein assembly driven by metal and chelating amino acids with selectivity and tunability
Article Snippet: .. Protein concentration was determined by measuring the absorbance at 280 nm (UV–vis spectrophotometer, Agilent Technologies Cary 8454 UV–vis) by using the epsilon values of protein and bipyridine at 280 nm . ..

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    Agilent technologies 8453 diode array spectrophotometer
    Small acceptance angle spectra comparing purified red cells and hemoglobin in solution acquired from an Agilent <t>8453</t> spectrophotometer with an acceptance angle of 2°. The RBCs were purified by washing whole blood by centrifugation and passing the suspension through a leuko-reduction filter. The RBC concentration is approximately 4000 cells/μl. The spectrum of the hemoglobin solution represents a concentration of ~0.12 mg/ml.
    8453 Diode Array Spectrophotometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8453 diode array spectrophotometer/product/Agilent technologies
    Average 93 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    8453 diode array spectrophotometer - by Bioz Stars, 2020-10
    93/100 stars
      Buy from Supplier

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    Small acceptance angle spectra comparing purified red cells and hemoglobin in solution acquired from an Agilent 8453 spectrophotometer with an acceptance angle of 2°. The RBCs were purified by washing whole blood by centrifugation and passing the suspension through a leuko-reduction filter. The RBC concentration is approximately 4000 cells/μl. The spectrum of the hemoglobin solution represents a concentration of ~0.12 mg/ml.

    Journal: Biomedical Optics Express

    Article Title: Hypochromicity in red blood cells: an experimental and theoretical investigation

    doi: 10.1364/BOE.2.002126

    Figure Lengend Snippet: Small acceptance angle spectra comparing purified red cells and hemoglobin in solution acquired from an Agilent 8453 spectrophotometer with an acceptance angle of 2°. The RBCs were purified by washing whole blood by centrifugation and passing the suspension through a leuko-reduction filter. The RBC concentration is approximately 4000 cells/μl. The spectrum of the hemoglobin solution represents a concentration of ~0.12 mg/ml.

    Article Snippet: The Agilent 8453 diode array spectrophotometer was used to acquire a majority of the data and has an acceptance angle of approximately 2°.

    Techniques: Purification, Spectrophotometry, Centrifugation, Concentration Assay