Journal: Frontiers in Neuroscience

Article Title: Melatonin receptors and Per1 expression in the inferior olivary nucleus of the Sapajus apella monkey

doi: 10.3389/fnins.2022.1072772

Figure Lengend Snippet: Immunofluorescence photomicrographs of the frontal section of the inferior olivary nucleus (ION) of the primate Sapajus apella showing Per1 – IR cells in (A) . Mean of the number of immunoreactive cells to Per1 in 3 brain sections representative of anteroposterior extension of the inferior olivary nucleus (ION) of the primate Sapajus apella ( N = 3 ZT). Photomicrograph of the cytoarchitecture characterization of neuronal populations of the ION represented by fluorescent staining in DAPI (blue) in A1 and B1, overlapping with A. Per-1 antibody showed the specificity of labeling in neuronal populations, allowing the delineation of the ION (A,B) . Per1 in neuronal populations of the ION (C) . Relative number of day and night Per1-IR cells by the surface area of the ION (D) . Intensity of staining - OD (E) . Bar = 100 μm.

Article Snippet: The sections were washed using a solution TBS-TX buffer (0.05 M), incubated for 48 h at 4°C in a solution containing 0.05 M TBS-TX buffer, 2% normal serum (Vector Laboratories, USA), and the appropriate primary antibody: anti-MT1 (1: 200, Santa Cruz), anti-MT2 (1: 200, Santa Cruz), anti-Per1 (1:500, Santa Cruz, USA) separately.

Techniques: Immunofluorescence, Staining, Labeling

Journal: Breast Cancer : Basic and Clinical Research

Article Title: Oscillation of Clock and Clock Controlled Genes Induced by Serum Shock in Human Breast Epithelial and Breast Cancer Cells: Regulation by Melatonin

doi: 10.4137/BCBCR.S9673

Figure Lengend Snippet: Primers of clock and clock controlled genes for qPCR.

Article Snippet: Monoclonal antibodies against human CLOCK, BMAL1, PER1, PER2, CRY1 and CRY2 proteins were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), and a mouse antibody against GAPDH was purchased from Sigma Chemical Co. (St. Louis, MO).

Techniques:

Journal: Breast Cancer : Basic and Clinical Research

Article Title: Oscillation of Clock and Clock Controlled Genes Induced by Serum Shock in Human Breast Epithelial and Breast Cancer Cells: Regulation by Melatonin

doi: 10.4137/BCBCR.S9673

Figure Lengend Snippet: Per2 knockdown in MCF-10A cells alters clock gene and CCG expression. MCF-10A Per2 knockdown or control cell lines were generated by infecting MCF-10A cells with Per2 shRNA or control lentiviral particles (Santa Cruz Biotechnology) according to the vendor’s protocol. ( A ) Stably infected cell lines were tested by Western blotting to determine the level of Per2 knockdown, with GAPDH expression used as a measure of protein loading. ( B ) Control and Per2 knockdown MCF-10A cells were plated as described in Materials and Methods and cell proliferation was determined by counting the number number of cells on a heamoctyometer after 4 days. ( C ) qPCR expression of clock and CCGs was determined as control and Per2 knockdown MCF-10A cells were harvested, mRNA extracted, and the clock gene expression for Clock, Bmal1, Per1, Cry1, Cry2, Rev-erbα and clock controlled genes MT 1 , c-Myc, and Sirt1 were analyzed by qPCR. Notes: * P < 0.05 , n = 3.

Article Snippet: Monoclonal antibodies against human CLOCK, BMAL1, PER1, PER2, CRY1 and CRY2 proteins were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), and a mouse antibody against GAPDH was purchased from Sigma Chemical Co. (St. Louis, MO).

Techniques: Expressing, Generated, shRNA, Stable Transfection, Infection, Western Blot

Journal: PLoS ONE

Article Title: A Circadian Clock in the Olfactory Bulb Anticipates Feeding during Food Anticipatory Activity

doi: 10.1371/journal.pone.0047779

Figure Lengend Snippet: Photomicrographs showing expression of FOS (A, B) and PER1 (C,D) in nursed subjects at 02∶00 (A,C) or 10∶00 (B,D) h. Note that FOS is high at time of nursing (ZT0) and 1.5 h later (ZT1.5), and PER1 is high 8 h after nursing (ZT08). Dotted lines delimit the layers. G, Granular cell layer; M, Mitral cell layer; PG, periglomerular cell layer. Rectangles represent the counted area. Scale bar, 100 μm.

Article Snippet: Free floating sections were incubated in PER1 antibody raised in goat (sc-7724, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or in c-Fos antibody raised in goat (sc-52G, Santa Cruz Biotechnology, Santa Cruz, CA, USA) both diluted at 1∶2000, in 3% normal horse serum and 0.3% Triton X-100 (Sigma, St. Louis MO, USA), for 48 h at 4°C.

Techniques: Expressing

Journal: PLoS ONE

Article Title: A Circadian Clock in the Olfactory Bulb Anticipates Feeding during Food Anticipatory Activity

doi: 10.1371/journal.pone.0047779

Figure Lengend Snippet: Graphs show PER1 expression in the periglomerular, mitral and granular layers in pups nursed and fasted during the night (A, C, E) or during the day (B, D, F). The acrophase of PER1 expression was 4–8 h after nursing time. Values are mean ± SEM. * Significant difference between highest and lowest values in each group. **, P<0.01 (see text for details).

Article Snippet: Free floating sections were incubated in PER1 antibody raised in goat (sc-7724, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or in c-Fos antibody raised in goat (sc-52G, Santa Cruz Biotechnology, Santa Cruz, CA, USA) both diluted at 1∶2000, in 3% normal horse serum and 0.3% Triton X-100 (Sigma, St. Louis MO, USA), for 48 h at 4°C.

Techniques: Expressing

Journal: PLoS ONE

Article Title: A Circadian Clock in the Olfactory Bulb Anticipates Feeding during Food Anticipatory Activity

doi: 10.1371/journal.pone.0047779

Figure Lengend Snippet: Photomicrographs showing expression of FOS (A) and PER1 (B) at times of high (ZT1.5; ZT08) and low (ZT20) proteins expression. Dotted lines delimit the mitral cell layer (M). Scale bar, 100 μm.

Article Snippet: Free floating sections were incubated in PER1 antibody raised in goat (sc-7724, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or in c-Fos antibody raised in goat (sc-52G, Santa Cruz Biotechnology, Santa Cruz, CA, USA) both diluted at 1∶2000, in 3% normal horse serum and 0.3% Triton X-100 (Sigma, St. Louis MO, USA), for 48 h at 4°C.

Techniques: Expressing

Journal: PLoS ONE

Article Title: A Circadian Clock in the Olfactory Bulb Anticipates Feeding during Food Anticipatory Activity

doi: 10.1371/journal.pone.0047779

Figure Lengend Snippet: Graphs shows FOS (A, B) and PER1 (C, D) in the mitral cell layer in pups nursed and fasted during the night (A, C) or during the day (B, D). Note that FOS expression is high at the time of nursing and increases further 1.5 h later. PER1 expression has a rhythm only in RF10∶00 group with an increase 8 h after nursing. Values are mean ± SEM. * Significant difference between highest and lowest values in each group. + + Significant difference of ZT0 and ZT04 time points against the lowest values. **, ++, P<0.01 (see text for details).

Article Snippet: Free floating sections were incubated in PER1 antibody raised in goat (sc-7724, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or in c-Fos antibody raised in goat (sc-52G, Santa Cruz Biotechnology, Santa Cruz, CA, USA) both diluted at 1∶2000, in 3% normal horse serum and 0.3% Triton X-100 (Sigma, St. Louis MO, USA), for 48 h at 4°C.

Techniques: Expressing

Journal: Journal of Circadian Rhythms

Article Title: Diurnal and nutritional adjustments of intracellular Ca 2+ release channels and Ca 2+ ATPases associated with restricted feeding schedules in the rat liver

doi: 10.1186/1740-3391-11-8

Figure Lengend Snippet: Daily rhythm characteristics of plasma corticosterone and PER1 from rats under free access meal and restricted schedule determined by COSINOR test

Article Snippet: Antibodies against PER1 (sc-7724), IP 3 R1(sc-6093) and IP 3 R2 (sc-7278), SERCA2 (sc-8094), PMCA1 (sc-16488) and PMCA4 (sc-22080), and Actin as well as alkaline phosphatase (AP)-conjugated rabbit anti-goat and goat anti-mouse secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and the Ryanodine Receptor (ab9078) was from Millipore (MA, USA).

Techniques:

Journal: Journal of Circadian Rhythms

Article Title: Diurnal and nutritional adjustments of intracellular Ca 2+ release channels and Ca 2+ ATPases associated with restricted feeding schedules in the rat liver

doi: 10.1186/1740-3391-11-8

Figure Lengend Snippet: Serum corticosterone and PER1 protein levels are entrained by food access. Diurnal corticosterone levels in rats fed ad libitum (AL) and under RF are shown in panel A. Quantitative analysis of clock protein PER1 using 50 μg of cytosolic fraction from at least 4 individuals is represented in panel B. Each value was normalized using the housekeeping protein actin as reference, and a representative western blot for each condition is shown. Black circles correspond to AL and white circles to RF group. The light gray rectangle above the x-axis represents mealtime for the food restricted group (ZT4-ZT6); * ( p < 0.05) means significant difference among AL group time points and + ( p < 0.05) significant difference among RF group time points (1-way ANOVA). # ( p < 0.05) significant difference between AL vs RF (2-way ANOVA).

Article Snippet: Antibodies against PER1 (sc-7724), IP 3 R1(sc-6093) and IP 3 R2 (sc-7278), SERCA2 (sc-8094), PMCA1 (sc-16488) and PMCA4 (sc-22080), and Actin as well as alkaline phosphatase (AP)-conjugated rabbit anti-goat and goat anti-mouse secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and the Ryanodine Receptor (ab9078) was from Millipore (MA, USA).

Techniques: Western Blot