Journal: PLoS ONE

Article Title: Identification of Protein Network Alterations upon Retinal Ischemia-Reperfusion Injury by Quantitative Proteomics Using a Rattus norvegicus Model

doi: 10.1371/journal.pone.0116453

Figure Lengend Snippet: The representative western blots of β-actin, albumin, calretinin, SYPH, SYT-1, Annexin A1, ApoA4, calnexin, GNAL, GFAP, H2B, HSP90AB1 and vimentin shown as the expression levels in the retinas (A) and as the densitometric quantitative results (B). Equal amounts of protein from the control retinas and the I/R-treated retinas were loaded. Each lane represents individual retina (n = 4 or 6 in each group; *p<0.05 compared with the non-injured retinas). The error bars represent the standard error of the mean. C, Retinal I/R-induced reductions of the synaptic proteins SYPH and SYT-1. The representative western blots of synaptophysin (SYPH) and synaptotagmin-1 (SYT-1) shown as the expression levels in the retinas are seen in (A). The stained retinal sections of SYPH (B) and SYT-1 (C) are shown at 2 days after the injury. Blue color: DAPI stained nuclei as control. Red color: SYPH or SYT-1 positively stained synapses. The scale bar represents 50 µm. C, non-injured eyes; I/R, I/R-injured eyes.

Article Snippet: Five microgram of the proteins from in vivo and in vitro samples were fractionated by SDS-PAGE, electroblotted onto a PVDF membrane (Millipore, Billerica, MA) and probed with different antibodies: anti-Calretinin (1∶5000 dilution, #2449-1, Abcam, CA), anti-actin (1∶2000 dilution, #CW0096B, CWBIO, China), anti-Albumin (1∶10000 dilution, 16475-1-AP, Proteintech, China), anti-synaptophysin (SYPH) (1∶50000 dilution, #1485-1, Abcam, CA), anti-SYT1 (1∶10000 dilution, 14511-1-AP, Proteintech, China), anti-mTOR (1∶1000 dilution, #2983, CST, MA), anti-phospho-mTOR (Ser2448) (1∶1000 dilution, #2971, CST, MA), anti-phospho-p70 S6 Kinase (Ser371) (1∶1000 dilution, #9208, CST, MA), anti-4E-BP1 (1∶1000 dilution, #9644, CST, MA), anti-phospho-4E-BP1 (Thr37/46) (1∶1000 dilution, #2855, CST, MA), anti-GFAP (1∶5000 dilution, #7260, Abcam, CA), anti-vimentin (1∶10000 dilution, #92560, Millipore, MA), anti-ApoA4 (1∶3000 dilution, #5700, CST, MA), anti-GNAL (1∶2000 dilution, #DF4108, Affinity, OH), anti-HSP90AB1 (1∶1000 dilution, #A1087, ABclonal, MA), anti-H2B (1∶1000 dilution, #8135, CST, MA), anti-Annexin A1 (1∶2000 dilution, 21990-1-AP, Proteintech, China) and anti-calnexin (1∶5000 dilution, #2692-1, Abcam, CA).

Techniques: Western Blot, Expressing, Staining

Journal: Molecular cell

Article Title: Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency

doi: 10.1016/j.molcel.2021.06.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Histone H2B (V119) , Cell Signaling Technology , Cat# 8135; RRID: AB_10891053.

Techniques: Recombinant, Electron Microscopy, Plasmid Preparation, Imaging, CCK-8 Assay, CRISPR, shRNA, Negative Control, Construct, Software, Transfection, DNA Extraction