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np ibv  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology np ibv
Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus <t>NP</t> <t>(NP(IAV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP <t>(NP(IBV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).
Np Ibv, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/np ibv/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
np ibv - by Bioz Stars, 2025-06
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sc 57885  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sc 57885
Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus <t>NP</t> <t>(NP(IAV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP <t>(NP(IBV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).
Sc 57885, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sc 57885/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
sc 57885 - by Bioz Stars, 2025-06
93/100 stars
  Buy from Supplier
mouse anti np antibody  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mouse anti np antibody
Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus <t>NP</t> <t>(NP(IAV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP <t>(NP(IBV),</t> teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).
Mouse Anti Np Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti np antibody/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
mouse anti np antibody - by Bioz Stars, 2025-06
93/100 stars
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Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus NP (NP(IAV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP (NP(IBV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).

Journal: Journal of Virology

Article Title: Influenza A virus NS1 effector domain is required for PA-X-mediated host shutoff in infected cells

doi: 10.1128/jvi.01901-23

Figure Lengend Snippet: Nuclear poly(A) RNA accumulation in infected cells does not require PA-X activity or nuclear PABPC1 redistribution. ( A ) MAVS-deficient A549 cells were mock infected or infected with influenza A virus (A/Cal/7) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza A virus NP (NP(IAV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( B ) MAVS-deficient A549 cells were mock infected or infected with influenza B virus (B/Bbris/60) at an MOI of 1 and analyzed by immunoFISH microscopy at 20 hpi. Cells were co-stained using antibodies for influenza B virus NP (NP(IBV), teal), PABPC1 (yellow), and fluorescently labeled oligo(dT) probe (poly(A), magenta). Scale bar = 50 µm. ( C ) Quantification of mock-infected, A/Cal/7, and B/Bris/60-infected cells with nuclear PABPC1 ( N = 3). ( D ) Nuclear to cytoplasmic intensity ratio for poly(A) RNA signal ( N = 3). In C and D, each datapoint represents values obtained from a randomly selected microscopy image containing at least 20 cells (three images analyzed per each independent biological replicate). In all graphs, one-way ANOVA and Tukey multiple comparisons tests were done to determine statistical significance (ns: non-significant; **** P -value < 0.0001; ** P -value < 0.01).

Article Snippet: Briefly, cells grown on 18 mm round coverslips were fixed with 4% paraformaldehyde in PBS for 15 min at ambient temperature and permeabilized with cold methanol for 10 min. After 1 h blocking with 5% bovine serum albumin (BSA, BioShop, Burlington, ON, Canada) in PBS, staining was performed overnight at +4⁰C with antibodies to the following targets: influenza A virus (IAV) polyclonal antibody (1:400, goat, Abcam, ab20841), NP (IAV) (1:1,000, mouse, Santa Cruz, sc-101352), NP (IBV) (1:200, mouse, Santa Cruz Biotechnology, sc-57885), PABPC1 (1:1000, rabbit, Abcam, ab21060), PABPN1 (1:200, rabbit, Abcam, ab75855), SON (1:600, rabbit, Abcam, ab121759), and SR proteins (1:100, mouse, Santa Cruz Biotechnology, sc-13509).

Techniques: Infection, Activity Assay, Virus, Microscopy, Staining, Labeling