rna  (Qiagen)


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    Name:
    AllPrep DNA RNA FFPE Kit
    Description:
    For simultaneous purification of genomic DNA and total RNA including small RNAs from formalin fixed paraffin embedded tissue sections Kit contents Qiagen AllPrep DNA RNA FFPE Kit 50 preps FFPE Tissue Sample RNA 14 to 30L DNA 30 to 100L Elution Volume Silica Technology Manual Processing For Simultaneous Purification of Genomic DNA and Total RNA from Formalin fixed Paraffin embedded Tissue Sections Ideal for PCR qPCR Real time RT PCR Microarray Includes 50 RNeasy minElute Spin Columns 50 QIAamp minElute Spin Columns Collection Tubes RNase free Reagents and Buffers Benefits Maximum output with minimal sample consumption Releases DNA RNA without compromising integrity Effectively separates RNA and DNA Comprehensive DNA and RNA analysis of the same FFPE sam
    Catalog Number:
    80234
    Price:
    660
    Category:
    AllPrep DNA RNA FFPE Kit
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    Structured Review

    Qiagen rna
    AllPrep DNA RNA FFPE Kit
    For simultaneous purification of genomic DNA and total RNA including small RNAs from formalin fixed paraffin embedded tissue sections Kit contents Qiagen AllPrep DNA RNA FFPE Kit 50 preps FFPE Tissue Sample RNA 14 to 30L DNA 30 to 100L Elution Volume Silica Technology Manual Processing For Simultaneous Purification of Genomic DNA and Total RNA from Formalin fixed Paraffin embedded Tissue Sections Ideal for PCR qPCR Real time RT PCR Microarray Includes 50 RNeasy minElute Spin Columns 50 QIAamp minElute Spin Columns Collection Tubes RNase free Reagents and Buffers Benefits Maximum output with minimal sample consumption Releases DNA RNA without compromising integrity Effectively separates RNA and DNA Comprehensive DNA and RNA analysis of the same FFPE sam
    https://www.bioz.com/result/rna/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2021-03
    99/100 stars

    Images

    1) Product Images from "Multiscale, multimodal analysis of tumor heterogeneity in IDH1 mutant vs wild-type diffuse gliomas"

    Article Title: Multiscale, multimodal analysis of tumor heterogeneity in IDH1 mutant vs wild-type diffuse gliomas

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0219724

    Comprehensive rendering of multiscale measurements in gliomas. Multiscale modalities depicted include: 1) clinical information (red), 2) IDH1 mutational status (blue), 3) MRI derived variables (green), 4) RNA expression level of genes involved in the “inducing angiogenesis” hallmark (black), and 5) MxIF angiogenesis markers or cell clusters (magenta). The data is binned in low, medium and high categories. Across the treatment-naïve gliomas (a) and the recurrent (post-treatment) glioblastoma* (b) cohorts, it can be observed that IDHmt patients have low angiogenesis according to RNA expression levels and expression of S100A4 and VEGRF. Those subjects also have high fraction of cells in clusters 1 and 2 (low angiogenesis markers), and low fraction of cells in cluster 4 and 5 (high angiogenesis markers ( S8 Fig , cluster profiles of angiogenesis clusters). Moreover, MR Images for the same subjects have lower normalized enhancing cores volumes and measure higher intensities on T1 post contrast. *Recurrent GBM (5 subjects are not shown since they were missing MxIF).
    Figure Legend Snippet: Comprehensive rendering of multiscale measurements in gliomas. Multiscale modalities depicted include: 1) clinical information (red), 2) IDH1 mutational status (blue), 3) MRI derived variables (green), 4) RNA expression level of genes involved in the “inducing angiogenesis” hallmark (black), and 5) MxIF angiogenesis markers or cell clusters (magenta). The data is binned in low, medium and high categories. Across the treatment-naïve gliomas (a) and the recurrent (post-treatment) glioblastoma* (b) cohorts, it can be observed that IDHmt patients have low angiogenesis according to RNA expression levels and expression of S100A4 and VEGRF. Those subjects also have high fraction of cells in clusters 1 and 2 (low angiogenesis markers), and low fraction of cells in cluster 4 and 5 (high angiogenesis markers ( S8 Fig , cluster profiles of angiogenesis clusters). Moreover, MR Images for the same subjects have lower normalized enhancing cores volumes and measure higher intensities on T1 post contrast. *Recurrent GBM (5 subjects are not shown since they were missing MxIF).

    Techniques Used: Magnetic Resonance Imaging, Derivative Assay, RNA Expression, Expressing

    Related Articles

    Formalin-fixed Paraffin-Embedded:

    Article Title: Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples
    Article Snippet: For DNA, the AllPrep DNA/RNA FFPE Kit, GeneRead DNA FFPE Kit and QIAamp DNA FFPE Tissue Kit from QIAGEN and truXTRAC FFPE DNA Kit from Covaris were used. .. For RNA, the AllPrep DNA/RNA FFPE Kit and RNeasy FFPE Kit from QIAGEN, Agencourt FormaPure Kit from Beckman Coulter and truXTRAC FFPE RNA Kit from Covaris were used. .. For the AllPrep DNA/RNA FFPE Kit, simultaneous extraction of DNA and RNA was done.

    Article Title: Multiscale, multimodal analysis of tumor heterogeneity in IDH1 mutant vs wild-type diffuse gliomas
    Article Snippet: .. For the eight fresh frozen glioma samples, Qiagen AllPrep DNA/RNA Mini Kit (cat#80204) was used to isolate DNA and RNA; for the four FFPE treatment-naïve glioma samples, Qiagen AllPrep DNA/RNA FFPE Kit (cat# 80234) was used. .. Exome libraries were constructed from 200ng of DNA (DIN = 3–5 for FFPE samples, DIN > 8 for blood and fresh frozen samples) using KAPA Biosystems’ Hyper Prep Kit (cat#KK8504) and Agilent’s SureSelectXT V5 baits, containing custom content, following the manufacturer’s protocols.

    Article Title: Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples
    Article Snippet: The major difference between these two protocols is the use of the Covaris Adaptive Focused Acoustics technology, using sonication to facilitate emulsification of paraffin and rehydrate the tissue for DNA extraction without using harsh organic solvents, suggesting the negative impact of organic solvents and method of paraffin removal on overall yield of material. .. We would also like to highlight that QIAGEN offers the AllPrep DNA/RNA FFPE Kit for concurrent extraction of DNA and RNA, and while the AllPrep protocol was not used in this study, technical documentation reports similar yields of material as the miRNeasy FFPE Kit. .. RNA integrity is inherently compromised by FFPE preservation, which is reflected by the RIN value.

    Article Title: Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples
    Article Snippet: DNA and RNA yieldDNA and RNA were extracted from the same archival FFPE blocks from five sarcoma samples using different extraction kits from commercial vendors in order to determine the best extraction method for downstream HTS applications. .. The QIAamp DNA FFPE Tissue Kit, miRNeasy FFPE Kit and AllPrep DNA/RNA FFPE Kit from QIAGEN have been shown to perform well in comparison with other DNA and RNA protocols in previous studies [ , , ]. .. Equal amount of starting material and a randomized order of the consecutive FFPE block sections were used for all methods of extraction.

    Article Title: Quantity and quality of nucleic acids extracted from archival formalin fixed paraffin embedded prostate biopsies
    Article Snippet: .. In the second trial of comparisons, the RNeasy® FFPE kit was compared to the RNA fraction of the samples extracted with Qiagen’s AllPrep® DNA/RNA FFPE kit. .. There was no evidence of difference in yields (11.4 ng/μl versus 12.6 ng/μl), A260/A280 ratios (1.47 versus 1.50) or CT -values (27.4 versus 28.0) between these two kits, although higher RIN-values were seen for samples extracted with the RNeasy® FFPE kit (2.2 versus 1.2, p = 0.006)(Table ).

    Article Title: Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo
    Article Snippet: RNA/protein extraction from paraffin embedded tissues RNA/protein extraction was performed from formalin fixed paraffin-embedded (FFPE) muscular and femur tissues. .. RNA was extracted using an All prep DNA/RNA FFPE kit (QIAGEN, Hilden, Germany) for RT-PCR, and protein extraction was carried out using a Q proteome FFPE tissue kit (QIAGEN) following manufacturer's instructions for immunoblotting. .. Statistical analysis Statistical analysis was performed using Prism 4.02 (GraphPad Software, San Diego, CA, USA).

    Article Title: Organocatalytic Removal of Formaldehyde Adducts from RNA and DNA Bases
    Article Snippet: .. RNA recovery from FFPE specimens RNA was extracted from a FFPE Raji cell pellet using either the spin-column-based AllPrep® DNA/RNA FFPE kit (Qiagen), according to the manufacturer’s protocol, or a phenol-chloroform-isoamyl alcohol (PCI) extraction procedure. ..

    Article Title: Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples
    Article Snippet: Five micron slices of FFPE specimens were dewaxed using Deparaffinization Solution (Qiagen, Valencia, CA). .. DNA extractions were done using Qiagen All Prep DNA/RNA FFPE Kit (Qiagen, Valencia CA), according to Qiagen’s supplementary protocol for FFPE tissue (QIAamp DNA FFPE Tissue Handbook). .. We implemented the following protocol changes: All FFPE samples were digested with 20 μL Proteinase K shaking overnight at 56 °C and then supplemented with an additional 15 μL for an additional hour for complete protein digestion.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo
    Article Snippet: RNA/protein extraction from paraffin embedded tissues RNA/protein extraction was performed from formalin fixed paraffin-embedded (FFPE) muscular and femur tissues. .. RNA was extracted using an All prep DNA/RNA FFPE kit (QIAGEN, Hilden, Germany) for RT-PCR, and protein extraction was carried out using a Q proteome FFPE tissue kit (QIAGEN) following manufacturer's instructions for immunoblotting. .. Statistical analysis Statistical analysis was performed using Prism 4.02 (GraphPad Software, San Diego, CA, USA).

    Protein Extraction:

    Article Title: Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo
    Article Snippet: RNA/protein extraction from paraffin embedded tissues RNA/protein extraction was performed from formalin fixed paraffin-embedded (FFPE) muscular and femur tissues. .. RNA was extracted using an All prep DNA/RNA FFPE kit (QIAGEN, Hilden, Germany) for RT-PCR, and protein extraction was carried out using a Q proteome FFPE tissue kit (QIAGEN) following manufacturer's instructions for immunoblotting. .. Statistical analysis Statistical analysis was performed using Prism 4.02 (GraphPad Software, San Diego, CA, USA).

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    Qiagen all prep dna rna ffpe kit
    Fraction of reads mapping to various parts of the genome. Ribosomal <t>RNA-depleted</t> total RNA library preparation for <t>FFPE</t> RNA resulted in larger fraction of reads mapping to intronic regions (both absolute and relative fraction) compared to mRNA library preparation for FF RNA.
    All Prep Dna Rna Ffpe Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/all prep dna rna ffpe kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    all prep dna rna ffpe kit - by Bioz Stars, 2021-03
    99/100 stars
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    Image Search Results


    Fraction of reads mapping to various parts of the genome. Ribosomal RNA-depleted total RNA library preparation for FFPE RNA resulted in larger fraction of reads mapping to intronic regions (both absolute and relative fraction) compared to mRNA library preparation for FF RNA.

    Journal: Scientific Reports

    Article Title: Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples

    doi: 10.1038/srep12335

    Figure Lengend Snippet: Fraction of reads mapping to various parts of the genome. Ribosomal RNA-depleted total RNA library preparation for FFPE RNA resulted in larger fraction of reads mapping to intronic regions (both absolute and relative fraction) compared to mRNA library preparation for FF RNA.

    Article Snippet: DNA extractions were done using Qiagen All Prep DNA/RNA FFPE Kit (Qiagen, Valencia CA), according to Qiagen’s supplementary protocol for FFPE tissue (QIAamp DNA FFPE Tissue Handbook).

    Techniques: Formalin-fixed Paraffin-Embedded

    Clustered heat map to visualize correlation matrix. Heat map of clustered correlation matrix (RNA-seq NanoString data sets from paired FF FFPE samples) shows strong correlation in gene expression between paired FF and FFPE samples. Color key was adjusted to minimal and maximal values to differentiate the differences. Dendrogram illustrates the relationship-distance between samples. Associated samples (e.g. FF2474 and FFPE2474) from the data sets produced with the same technology (either RNA sequencing or NanoString) are highlighted with a white frame, whereas associated samples from different technologies are highlighted with a blue frame. Sample NS_FF3356r1.NS_FF4079r1 was a replicate sample that was mislabeled as FF3356r1 during NanoString analysis. However, clustering analysis correctly identified it as FF4079r1. Note that correctly labeled FF3356r1 clustered with other FF3356 and FFPE3356 replicates.

    Journal: Scientific Reports

    Article Title: Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples

    doi: 10.1038/srep12335

    Figure Lengend Snippet: Clustered heat map to visualize correlation matrix. Heat map of clustered correlation matrix (RNA-seq NanoString data sets from paired FF FFPE samples) shows strong correlation in gene expression between paired FF and FFPE samples. Color key was adjusted to minimal and maximal values to differentiate the differences. Dendrogram illustrates the relationship-distance between samples. Associated samples (e.g. FF2474 and FFPE2474) from the data sets produced with the same technology (either RNA sequencing or NanoString) are highlighted with a white frame, whereas associated samples from different technologies are highlighted with a blue frame. Sample NS_FF3356r1.NS_FF4079r1 was a replicate sample that was mislabeled as FF3356r1 during NanoString analysis. However, clustering analysis correctly identified it as FF4079r1. Note that correctly labeled FF3356r1 clustered with other FF3356 and FFPE3356 replicates.

    Article Snippet: DNA extractions were done using Qiagen All Prep DNA/RNA FFPE Kit (Qiagen, Valencia CA), according to Qiagen’s supplementary protocol for FFPE tissue (QIAamp DNA FFPE Tissue Handbook).

    Techniques: RNA Sequencing Assay, Formalin-fixed Paraffin-Embedded, Expressing, Produced, Labeling

    Variant allele frequencies and FFPE artifacts. Panel A, B and D show the comparison of variant allele fraction in FF or FFPE RNA (left y-axis) and FF DNA (right y-axis). Each line represents the relationship of one variant in comparison. The color code indicates whether the mutations is concordant (green), discordant (red), or ambiguous (black) because of low DNA coverage. Panel A shows the comparison between RNA with DNA from fresh frozen sample (2474). Most of the variants detected in RNA samples are concordant with variant calls from DNA. No discordant calls were observed. Eighteen variant calls from RNA sequence data were considered ambiguous because the same variants were not called in DNA due to low coverage. Panel B shows the comparison of variant allele fraction in RNA and DNA from paired FFPE and FF samples (2474), respectively. High degree of discordant calls were observed. Panel C shows the distribution of various substitutions in FF and FFPE samples. Substitutions that are concordant between paired FF and FFPE samples are indicated by green and discordant substitutions are indicated by red. The majority of discordant calls are observed as C > T or G > A substitutions, suggesting a documented FFPE artifact. Results from Panel D indicate that a small fraction of discordant calls remained after removing the C > T or G > A substitutions present at

    Journal: Scientific Reports

    Article Title: Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples

    doi: 10.1038/srep12335

    Figure Lengend Snippet: Variant allele frequencies and FFPE artifacts. Panel A, B and D show the comparison of variant allele fraction in FF or FFPE RNA (left y-axis) and FF DNA (right y-axis). Each line represents the relationship of one variant in comparison. The color code indicates whether the mutations is concordant (green), discordant (red), or ambiguous (black) because of low DNA coverage. Panel A shows the comparison between RNA with DNA from fresh frozen sample (2474). Most of the variants detected in RNA samples are concordant with variant calls from DNA. No discordant calls were observed. Eighteen variant calls from RNA sequence data were considered ambiguous because the same variants were not called in DNA due to low coverage. Panel B shows the comparison of variant allele fraction in RNA and DNA from paired FFPE and FF samples (2474), respectively. High degree of discordant calls were observed. Panel C shows the distribution of various substitutions in FF and FFPE samples. Substitutions that are concordant between paired FF and FFPE samples are indicated by green and discordant substitutions are indicated by red. The majority of discordant calls are observed as C > T or G > A substitutions, suggesting a documented FFPE artifact. Results from Panel D indicate that a small fraction of discordant calls remained after removing the C > T or G > A substitutions present at

    Article Snippet: DNA extractions were done using Qiagen All Prep DNA/RNA FFPE Kit (Qiagen, Valencia CA), according to Qiagen’s supplementary protocol for FFPE tissue (QIAamp DNA FFPE Tissue Handbook).

    Techniques: Variant Assay, Formalin-fixed Paraffin-Embedded, Sequencing

    GC content distribution before and after applying filter on FF and FFPE samples. The GC-content distribution indicates abnormal GC peak (blue line) in FFPE (A) but not in FF (B) samples. The GC content of the human reference genome is plotted in red line. To understand why GC content in FFPE sample show an abnormal peak, mapped reads were visualized by SeqMonk. The representative chromosome view shows three rows of data tracks for each sample: (1) mRNA.only contains only reads in mRNA regions, (2) not.mRNA contains all reads that did not align to exons, (3) total contains all reads. Strand information is shown in red and blue. FFPE RNA sequencing produces reads that also map to intronic regions. When reads mapping to intronic regions in both FF and FFPE were filtered out, abnormal GC peak in FFPE samples were removed (D) without affecting the GC-content distribution of FF samples (E).

    Journal: Scientific Reports

    Article Title: Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples

    doi: 10.1038/srep12335

    Figure Lengend Snippet: GC content distribution before and after applying filter on FF and FFPE samples. The GC-content distribution indicates abnormal GC peak (blue line) in FFPE (A) but not in FF (B) samples. The GC content of the human reference genome is plotted in red line. To understand why GC content in FFPE sample show an abnormal peak, mapped reads were visualized by SeqMonk. The representative chromosome view shows three rows of data tracks for each sample: (1) mRNA.only contains only reads in mRNA regions, (2) not.mRNA contains all reads that did not align to exons, (3) total contains all reads. Strand information is shown in red and blue. FFPE RNA sequencing produces reads that also map to intronic regions. When reads mapping to intronic regions in both FF and FFPE were filtered out, abnormal GC peak in FFPE samples were removed (D) without affecting the GC-content distribution of FF samples (E).

    Article Snippet: DNA extractions were done using Qiagen All Prep DNA/RNA FFPE Kit (Qiagen, Valencia CA), according to Qiagen’s supplementary protocol for FFPE tissue (QIAamp DNA FFPE Tissue Handbook).

    Techniques: Formalin-fixed Paraffin-Embedded, RNA Sequencing Assay

    Yield and amplifiability of extracted DNA and RNA. (A) Average total amount of DNA. (B) Average total amount of RNA. (C) Amplifiable DNA quantified with the FFPE QC kit from Illumina. (D) Amplifiable RNA quantified with the PreSeq QC assay from ArcherDx. The average total amount and average delta Ct values for the different samples and extraction methods are shown. The standard deviation is shown as vertical bars. Methods with significant differences in yield are marked as connected with horizontal bars (p

    Journal: PLoS ONE

    Article Title: Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples

    doi: 10.1371/journal.pone.0197456

    Figure Lengend Snippet: Yield and amplifiability of extracted DNA and RNA. (A) Average total amount of DNA. (B) Average total amount of RNA. (C) Amplifiable DNA quantified with the FFPE QC kit from Illumina. (D) Amplifiable RNA quantified with the PreSeq QC assay from ArcherDx. The average total amount and average delta Ct values for the different samples and extraction methods are shown. The standard deviation is shown as vertical bars. Methods with significant differences in yield are marked as connected with horizontal bars (p

    Article Snippet: The QIAamp DNA FFPE Tissue Kit, miRNeasy FFPE Kit and AllPrep DNA/RNA FFPE Kit from QIAGEN have been shown to perform well in comparison with other DNA and RNA protocols in previous studies [ , , ].

    Techniques: Formalin-fixed Paraffin-Embedded, Standard Deviation

    Comprehensive rendering of multiscale measurements in gliomas. Multiscale modalities depicted include: 1) clinical information (red), 2) IDH1 mutational status (blue), 3) MRI derived variables (green), 4) RNA expression level of genes involved in the “inducing angiogenesis” hallmark (black), and 5) MxIF angiogenesis markers or cell clusters (magenta). The data is binned in low, medium and high categories. Across the treatment-naïve gliomas (a) and the recurrent (post-treatment) glioblastoma* (b) cohorts, it can be observed that IDHmt patients have low angiogenesis according to RNA expression levels and expression of S100A4 and VEGRF. Those subjects also have high fraction of cells in clusters 1 and 2 (low angiogenesis markers), and low fraction of cells in cluster 4 and 5 (high angiogenesis markers ( S8 Fig , cluster profiles of angiogenesis clusters). Moreover, MR Images for the same subjects have lower normalized enhancing cores volumes and measure higher intensities on T1 post contrast. *Recurrent GBM (5 subjects are not shown since they were missing MxIF).

    Journal: PLoS ONE

    Article Title: Multiscale, multimodal analysis of tumor heterogeneity in IDH1 mutant vs wild-type diffuse gliomas

    doi: 10.1371/journal.pone.0219724

    Figure Lengend Snippet: Comprehensive rendering of multiscale measurements in gliomas. Multiscale modalities depicted include: 1) clinical information (red), 2) IDH1 mutational status (blue), 3) MRI derived variables (green), 4) RNA expression level of genes involved in the “inducing angiogenesis” hallmark (black), and 5) MxIF angiogenesis markers or cell clusters (magenta). The data is binned in low, medium and high categories. Across the treatment-naïve gliomas (a) and the recurrent (post-treatment) glioblastoma* (b) cohorts, it can be observed that IDHmt patients have low angiogenesis according to RNA expression levels and expression of S100A4 and VEGRF. Those subjects also have high fraction of cells in clusters 1 and 2 (low angiogenesis markers), and low fraction of cells in cluster 4 and 5 (high angiogenesis markers ( S8 Fig , cluster profiles of angiogenesis clusters). Moreover, MR Images for the same subjects have lower normalized enhancing cores volumes and measure higher intensities on T1 post contrast. *Recurrent GBM (5 subjects are not shown since they were missing MxIF).

    Article Snippet: For the eight fresh frozen glioma samples, Qiagen AllPrep DNA/RNA Mini Kit (cat#80204) was used to isolate DNA and RNA; for the four FFPE treatment-naïve glioma samples, Qiagen AllPrep DNA/RNA FFPE Kit (cat# 80234) was used.

    Techniques: Magnetic Resonance Imaging, Derivative Assay, RNA Expression, Expressing

    Histological analysis at 12 weeks post-implantation intramuscularly (A). In H E staining, peripheral regions of the bioceramic-implanted muscles showed variable degrees of newly formed eosinophilic connective tissue deposition, which show a positive reaction to trichrome staining indicating a collagen-rich stroma. All bioceramic-implanted groups showed positive immunohistochemistry reaction to ALP and type I collagen but differences of positive intensity between each group were not obvious. Immunohistochemistry, Mayer's hematoxylin counter staining, Magnification, ×200. RT-PCR (B, C) for gene and immunoblotting for protein (D) expression of osteogenic molecules in the intramuscularis and cortical defects. (B, C) Formalin fixed paraffin-embedded (FFPE) muscular and femur tissue RNA was extracted by All prep DNA/RNA FFPE kit. In muscles, mRNA expression of ALP was increased to a greater degree in the HA group than in others, with similar results observed by immunoblotting as shown in Figure 6B . Type I collagen was up-regulated in the CMP and HA groups. (D) Expression of ALP was higher in the HA group than in the others, however, there was no significant difference in the expression level of type I collagen among all groups. Protein extraction from FFPE muscular tissues by Q proteome FFPE tissue kit for immunoblotting. Values are the mean ± standard deviation. # p

    Journal: PLoS ONE

    Article Title: Comparative Characteristics of Porous Bioceramics for an Osteogenic Response In Vitro and In Vivo

    doi: 10.1371/journal.pone.0084272

    Figure Lengend Snippet: Histological analysis at 12 weeks post-implantation intramuscularly (A). In H E staining, peripheral regions of the bioceramic-implanted muscles showed variable degrees of newly formed eosinophilic connective tissue deposition, which show a positive reaction to trichrome staining indicating a collagen-rich stroma. All bioceramic-implanted groups showed positive immunohistochemistry reaction to ALP and type I collagen but differences of positive intensity between each group were not obvious. Immunohistochemistry, Mayer's hematoxylin counter staining, Magnification, ×200. RT-PCR (B, C) for gene and immunoblotting for protein (D) expression of osteogenic molecules in the intramuscularis and cortical defects. (B, C) Formalin fixed paraffin-embedded (FFPE) muscular and femur tissue RNA was extracted by All prep DNA/RNA FFPE kit. In muscles, mRNA expression of ALP was increased to a greater degree in the HA group than in others, with similar results observed by immunoblotting as shown in Figure 6B . Type I collagen was up-regulated in the CMP and HA groups. (D) Expression of ALP was higher in the HA group than in the others, however, there was no significant difference in the expression level of type I collagen among all groups. Protein extraction from FFPE muscular tissues by Q proteome FFPE tissue kit for immunoblotting. Values are the mean ± standard deviation. # p

    Article Snippet: RNA was extracted using an All prep DNA/RNA FFPE kit (QIAGEN, Hilden, Germany) for RT-PCR, and protein extraction was carried out using a Q proteome FFPE tissue kit (QIAGEN) following manufacturer's instructions for immunoblotting.

    Techniques: Staining, Immunohistochemistry, ALP Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Formalin-fixed Paraffin-Embedded, Protein Extraction, Standard Deviation