8 oxodgtp  (Jena Bioscience)


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  • 94
    Name:
    8 Oxo dGTP
    Description:

    Catalog Number:
    NU-1117L
    Price:
    352.16
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 30 µl
    Buy from Supplier


    Structured Review

    Jena Bioscience 8 oxodgtp
    Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and <t>8-oxodGTP,</t> respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b Graph shows time-dependent changes in the products of insertion (pink and purple for 8-oxodGTP and dGTP insertions, respectively) and ligation (cyan and blue for ligation following insertion of 8-oxodGTP and dGTP, respectively). The data represent the average of four independent experiments ± SD. Corresponding uncropped gel image and presentation of the data in line graph format are shown in Supplementary Fig. 2

    https://www.bioz.com/result/8 oxodgtp/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    8 oxodgtp - by Bioz Stars, 2021-09
    94/100 stars

    Images

    1) Product Images from "Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate"

    Article Title: Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate

    Journal: Nature Communications

    doi: 10.1038/s41467-018-06700-5

    Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and 8-oxodGTP, respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b Graph shows time-dependent changes in the products of insertion (pink and purple for 8-oxodGTP and dGTP insertions, respectively) and ligation (cyan and blue for ligation following insertion of 8-oxodGTP and dGTP, respectively). The data represent the average of four independent experiments ± SD. Corresponding uncropped gel image and presentation of the data in line graph format are shown in Supplementary Fig. 2
    Figure Legend Snippet: Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and 8-oxodGTP, respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b Graph shows time-dependent changes in the products of insertion (pink and purple for 8-oxodGTP and dGTP insertions, respectively) and ligation (cyan and blue for ligation following insertion of 8-oxodGTP and dGTP, respectively). The data represent the average of four independent experiments ± SD. Corresponding uncropped gel image and presentation of the data in line graph format are shown in Supplementary Fig. 2

    Techniques Used: Ligation

    2) Product Images from "Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate"

    Article Title: Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate

    Journal: Nature Communications

    doi: 10.1038/s41467-018-06700-5

    Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and 8-oxodGTP, respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b
    Figure Legend Snippet: Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and 8-oxodGTP, respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b

    Techniques Used: Ligation

    Related Articles

    other:

    Article Title: Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration
    Article Snippet: dGTP, 8-oxo-dGTP, 8-oxo-GTP, and 8-oxo-dATP were purchased from Jena Biosciences GmbH (Jena, Germany), GTP was purchased from Promega (Madison, WI, United States), dATP, deoxyguanosine (dG), and 8-oxo-dG were purchased from Sigma-Aldrich (St. Louis, MO, United States).

    Construct:

    Article Title: Structural basis of the correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase
    Article Snippet: .. To construct a random mutant library from the nylC gene, pSKFC4–1 (having G122 Y130 A36 Q263 mutations in pSKFC4) was initially digested with Pst I and Bam HI, and the linearized DNA was amplified by PCR in the presence of nucleotide analogues (2, 5, 10, 40, or 70 μM concentrations of both 8-oxo-2′-deoxyguanosine-5′-triphosphate (8-oxo-dGTP) and 6H,8H-3,4-dihydropyrimido(4,5-C)(1,2)oxazin-7-one-8-β-D-2′ -deoxy-ribofuranoside-5′-triphosphate (dPTP)] (Jena Bioscience GmbH, Jena, Germany) using two primers, FE-BamHI and RE-PstI (Table ) , . ..

    Article Title: Engineering Stem Cell Factor Ligands with Different c-Kit Agonistic Potencies
    Article Snippet: .. A first-generation DNA library originating from SCFM (used as a template for error prone PCR) was constructed using error prone PCR with low fidelity Taq DNA polymerase (New England Biolabs) and 2 uM of 8-oxo dGTP and dPTP nucleotide analogs (Jena Bioscience, Jena, Germany). ..

    Mutagenesis:

    Article Title: Structural basis of the correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase
    Article Snippet: .. To construct a random mutant library from the nylC gene, pSKFC4–1 (having G122 Y130 A36 Q263 mutations in pSKFC4) was initially digested with Pst I and Bam HI, and the linearized DNA was amplified by PCR in the presence of nucleotide analogues (2, 5, 10, 40, or 70 μM concentrations of both 8-oxo-2′-deoxyguanosine-5′-triphosphate (8-oxo-dGTP) and 6H,8H-3,4-dihydropyrimido(4,5-C)(1,2)oxazin-7-one-8-β-D-2′ -deoxy-ribofuranoside-5′-triphosphate (dPTP)] (Jena Bioscience GmbH, Jena, Germany) using two primers, FE-BamHI and RE-PstI (Table ) , . ..

    Amplification:

    Article Title: Structural basis of the correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase
    Article Snippet: .. To construct a random mutant library from the nylC gene, pSKFC4–1 (having G122 Y130 A36 Q263 mutations in pSKFC4) was initially digested with Pst I and Bam HI, and the linearized DNA was amplified by PCR in the presence of nucleotide analogues (2, 5, 10, 40, or 70 μM concentrations of both 8-oxo-2′-deoxyguanosine-5′-triphosphate (8-oxo-dGTP) and 6H,8H-3,4-dihydropyrimido(4,5-C)(1,2)oxazin-7-one-8-β-D-2′ -deoxy-ribofuranoside-5′-triphosphate (dPTP)] (Jena Bioscience GmbH, Jena, Germany) using two primers, FE-BamHI and RE-PstI (Table ) , . ..

    Polymerase Chain Reaction:

    Article Title: Structural basis of the correct subunit assembly, aggregation, and intracellular degradation of nylon hydrolase
    Article Snippet: .. To construct a random mutant library from the nylC gene, pSKFC4–1 (having G122 Y130 A36 Q263 mutations in pSKFC4) was initially digested with Pst I and Bam HI, and the linearized DNA was amplified by PCR in the presence of nucleotide analogues (2, 5, 10, 40, or 70 μM concentrations of both 8-oxo-2′-deoxyguanosine-5′-triphosphate (8-oxo-dGTP) and 6H,8H-3,4-dihydropyrimido(4,5-C)(1,2)oxazin-7-one-8-β-D-2′ -deoxy-ribofuranoside-5′-triphosphate (dPTP)] (Jena Bioscience GmbH, Jena, Germany) using two primers, FE-BamHI and RE-PstI (Table ) , . ..

    Article Title: Engineering Stem Cell Factor Ligands with Different c-Kit Agonistic Potencies
    Article Snippet: .. A first-generation DNA library originating from SCFM (used as a template for error prone PCR) was constructed using error prone PCR with low fidelity Taq DNA polymerase (New England Biolabs) and 2 uM of 8-oxo dGTP and dPTP nucleotide analogs (Jena Bioscience, Jena, Germany). ..

    Incubation:

    Article Title: Biochemical and structural studies of Mycobacterium smegmatis MutT1, a sanitization enzyme with unusual modes of association.
    Article Snippet: .. Mycobacterium smegmatis MutT1, which is made up of a Nudix domain (domain 1) and a histidine phosphatase domain (domain 2), efficiently hydrolyses 8-oxo-GTP and 8-oxo-dGTP to the corresponding nucleoside diphosphates and phosphate in the presence of magnesium ions. ..

    Transferring:

    Article Title: In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair
    Article Snippet: .. Eppendorf tubes (1.5 ml) Pipette tips (10 μl, 100 μl, 1,000 μl) Deoxyguanosine triphosphate (dGTP) (New England Biolabs, catalog number: N0447S) 8-oxo-2′-deoxyguanosine-5′-Triphosphate (8-oxo-dGTP) (Jena Bioscience, catalog number: NU-1117L) DNA substrate: The DNA substrate includes a fluorescent tag at both the 5′- and 3′-ends of one of the gap-containing strand in the double-stranded DNA with a single nucleotide gap opposite template base Cytosine ( ) Note: The sequence information for the DNA substrate is presented in the Notes section of the protocol. ..

    Sequencing:

    Article Title: In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair
    Article Snippet: .. Eppendorf tubes (1.5 ml) Pipette tips (10 μl, 100 μl, 1,000 μl) Deoxyguanosine triphosphate (dGTP) (New England Biolabs, catalog number: N0447S) 8-oxo-2′-deoxyguanosine-5′-Triphosphate (8-oxo-dGTP) (Jena Bioscience, catalog number: NU-1117L) DNA substrate: The DNA substrate includes a fluorescent tag at both the 5′- and 3′-ends of one of the gap-containing strand in the double-stranded DNA with a single nucleotide gap opposite template base Cytosine ( ) Note: The sequence information for the DNA substrate is presented in the Notes section of the protocol. ..

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  • 94
    Jena Bioscience 8 oxodgtp
    Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and <t>8-oxodGTP,</t> respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b Graph shows time-dependent changes in the products of insertion (pink and purple for 8-oxodGTP and dGTP insertions, respectively) and ligation (cyan and blue for ligation following insertion of 8-oxodGTP and dGTP, respectively). The data represent the average of four independent experiments ± SD. Corresponding uncropped gel image and presentation of the data in line graph format are shown in Supplementary Fig. 2
    8 Oxodgtp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/8 oxodgtp/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    8 oxodgtp - by Bioz Stars, 2021-09
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    94
    Jena Bioscience base nucleotide
    Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and <t>8-oxodGTP,</t> respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b Graph shows time-dependent changes in the products of insertion (pink and purple for 8-oxodGTP and dGTP insertions, respectively) and ligation (cyan and blue for ligation following insertion of 8-oxodGTP and dGTP, respectively). The data represent the average of four independent experiments ± SD. Corresponding uncropped gel image and presentation of the data in line graph format are shown in Supplementary Fig. 2
    Base Nucleotide, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/base nucleotide/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    base nucleotide - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and 8-oxodGTP, respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b Graph shows time-dependent changes in the products of insertion (pink and purple for 8-oxodGTP and dGTP insertions, respectively) and ligation (cyan and blue for ligation following insertion of 8-oxodGTP and dGTP, respectively). The data represent the average of four independent experiments ± SD. Corresponding uncropped gel image and presentation of the data in line graph format are shown in Supplementary Fig. 2

    Journal: Nature Communications

    Article Title: Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate

    doi: 10.1038/s41467-018-06700-5

    Figure Lengend Snippet: Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and 8-oxodGTP, respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b Graph shows time-dependent changes in the products of insertion (pink and purple for 8-oxodGTP and dGTP insertions, respectively) and ligation (cyan and blue for ligation following insertion of 8-oxodGTP and dGTP, respectively). The data represent the average of four independent experiments ± SD. Corresponding uncropped gel image and presentation of the data in line graph format are shown in Supplementary Fig. 2

    Article Snippet: Briefly, the reaction mixture (10 μl in final volume) included 50 mM Tris(HCl), pH 7.5, 100 mM KCl, 10 mM MgCl2 , 1 mM ATP, 100 μg ml−1 BSA, 10% glycerol, 1 mM DTT, and 200 μM of 8-oxodGTP (Jena Bioscience), dGTP or dATP (NEB), 300 nM DNA substrate, and 100 nM enzyme mixture.

    Techniques: Ligation

    Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and 8-oxodGTP, respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b

    Journal: Nature Communications

    Article Title: Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate

    doi: 10.1038/s41467-018-06700-5

    Figure Lengend Snippet: Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and 8-oxodGTP, respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b

    Article Snippet: Briefly, the reaction mixture (10 μl in final volume) included 50 mM Tris(HCl), pH 7.5, 100 mM KCl, 10 mM MgCl2 , 1 mM ATP, 100 μg ml−1 BSA, 10% glycerol, 1 mM DTT, and 200 μM of 8-oxodGTP (Jena Bioscience), dGTP or dATP (NEB), 300 nM DNA substrate, and 100 nM enzyme mixture.

    Techniques: Ligation