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Difco 7h9 minimal medium
Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in <t>7H9</t> medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value
7h9 Minimal Medium, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/7h9 minimal medium/product/Difco
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
7h9 minimal medium - by Bioz Stars, 2020-04
94/100 stars

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1) Product Images from "A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response"

Article Title: A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2018.02800

Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in 7H9 medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value
Figure Legend Snippet: Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in 7H9 medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

Techniques Used: Mutagenesis, Quantitative RT-PCR, Software, Quantitation Assay, Expressing

Induction of the qsd operon expression by 3-oxo-C 8 -HSL. qRT-PCR analysis of the qsdA , qsdC , and qsdR transcription was conducted in the R. erythropolis R138 wild-type strain grown in 7H9 medium in which 3-oxo-C 8 -HSL was added at mid-exponential phase. Expression levels of qsdA , qsdC , and qsdR are relative to those obtained in the same medium without the inducer. The dotted line indicates the same mRNA expression between the induced condition and without the inducers. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value
Figure Legend Snippet: Induction of the qsd operon expression by 3-oxo-C 8 -HSL. qRT-PCR analysis of the qsdA , qsdC , and qsdR transcription was conducted in the R. erythropolis R138 wild-type strain grown in 7H9 medium in which 3-oxo-C 8 -HSL was added at mid-exponential phase. Expression levels of qsdA , qsdC , and qsdR are relative to those obtained in the same medium without the inducer. The dotted line indicates the same mRNA expression between the induced condition and without the inducers. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

Techniques Used: Expressing, Quantitative RT-PCR, Software, Quantitation Assay

2) Product Images from "A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response"

Article Title: A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2018.02800

Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in 7H9 medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value
Figure Legend Snippet: Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in 7H9 medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

Techniques Used: Mutagenesis, Quantitative RT-PCR, Software, Quantitation Assay, Expressing

Induction of the qsd operon expression by 3-oxo-C 8 -HSL. qRT-PCR analysis of the qsdA , qsdC , and qsdR transcription was conducted in the R. erythropolis R138 wild-type strain grown in 7H9 medium in which 3-oxo-C 8 -HSL was added at mid-exponential phase. Expression levels of qsdA , qsdC , and qsdR are relative to those obtained in the same medium without the inducer. The dotted line indicates the same mRNA expression between the induced condition and without the inducers. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value
Figure Legend Snippet: Induction of the qsd operon expression by 3-oxo-C 8 -HSL. qRT-PCR analysis of the qsdA , qsdC , and qsdR transcription was conducted in the R. erythropolis R138 wild-type strain grown in 7H9 medium in which 3-oxo-C 8 -HSL was added at mid-exponential phase. Expression levels of qsdA , qsdC , and qsdR are relative to those obtained in the same medium without the inducer. The dotted line indicates the same mRNA expression between the induced condition and without the inducers. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

Techniques Used: Expressing, Quantitative RT-PCR, Software, Quantitation Assay

Related Articles

Concentration Assay:

Article Title: A Flavor Lactone Mimicking AHL Quorum-Sensing Signals Exploits the Broad Affinity of the QsdR Regulator to Stimulate Transcription of the Rhodococcal qsd Operon Involved in Quorum-Quenching and Biocontrol Activities
Article Snippet: R. erythropolis strains were cultured in 7H9 minimal medium (Difco) supplemented with 6 mM hexanoate (Sigma-Aldrich) as the sole source of carbon for the induction and transcriptional fusion assays. .. When necessary, the following antibiotics were added to growth medium: kanamycin at a concentration of 200 μg/ml for R. erythropolis and ampicillin at a concentration of 100 μg/ml for E. coli .

other:

Article Title: A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response
Article Snippet: R. erythropolis strains were cultivated in LBP ( ) for conjugative transfer of DNA and in 7H9 minimal medium (Difco) supplemented with hexanoate at 6 mM (Sigma-Aldrich) as carbon source for induction assays.

Cell Culture:

Article Title: A Flavor Lactone Mimicking AHL Quorum-Sensing Signals Exploits the Broad Affinity of the QsdR Regulator to Stimulate Transcription of the Rhodococcal qsd Operon Involved in Quorum-Quenching and Biocontrol Activities
Article Snippet: .. R. erythropolis strains were cultured in 7H9 minimal medium (Difco) supplemented with 6 mM hexanoate (Sigma-Aldrich) as the sole source of carbon for the induction and transcriptional fusion assays. .. For induction experiments, GCL (Sigma-Aldrich) was added to 7H9 medium at concentrations of 1 μM to 6 mM when the bacteria reached the mid-exponential growth phase.

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    Difco 7h9 minimal medium
    Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in <t>7H9</t> medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value
    7h9 Minimal Medium, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 minimal medium/product/Difco
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    7h9 minimal medium - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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    Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in 7H9 medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

    Journal: Frontiers in Microbiology

    Article Title: A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response

    doi: 10.3389/fmicb.2018.02800

    Figure Lengend Snippet: Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in 7H9 medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

    Article Snippet: R. erythropolis strains were cultivated in LBP ( ) for conjugative transfer of DNA and in 7H9 minimal medium (Difco) supplemented with hexanoate at 6 mM (Sigma-Aldrich) as carbon source for induction assays.

    Techniques: Mutagenesis, Quantitative RT-PCR, Software, Quantitation Assay, Expressing

    Induction of the qsd operon expression by 3-oxo-C 8 -HSL. qRT-PCR analysis of the qsdA , qsdC , and qsdR transcription was conducted in the R. erythropolis R138 wild-type strain grown in 7H9 medium in which 3-oxo-C 8 -HSL was added at mid-exponential phase. Expression levels of qsdA , qsdC , and qsdR are relative to those obtained in the same medium without the inducer. The dotted line indicates the same mRNA expression between the induced condition and without the inducers. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

    Journal: Frontiers in Microbiology

    Article Title: A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response

    doi: 10.3389/fmicb.2018.02800

    Figure Lengend Snippet: Induction of the qsd operon expression by 3-oxo-C 8 -HSL. qRT-PCR analysis of the qsdA , qsdC , and qsdR transcription was conducted in the R. erythropolis R138 wild-type strain grown in 7H9 medium in which 3-oxo-C 8 -HSL was added at mid-exponential phase. Expression levels of qsdA , qsdC , and qsdR are relative to those obtained in the same medium without the inducer. The dotted line indicates the same mRNA expression between the induced condition and without the inducers. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

    Article Snippet: R. erythropolis strains were cultivated in LBP ( ) for conjugative transfer of DNA and in 7H9 minimal medium (Difco) supplemented with hexanoate at 6 mM (Sigma-Aldrich) as carbon source for induction assays.

    Techniques: Expressing, Quantitative RT-PCR, Software, Quantitation Assay

    Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in 7H9 medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

    Journal: Frontiers in Microbiology

    Article Title: A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response

    doi: 10.3389/fmicb.2018.02800

    Figure Lengend Snippet: Increased qsdA and qsdC mRNA levels in a Δ qsdR mutant strain. qRT-PCR analysis shows the qsdA and qsdC mRNA levels in the Δ qsdR mutant and in the complementing strain (Δ qsdR-qsdR ) grown in 7H9 medium at the mid-exponential (10 h) and stationary (19 h) phases. These mRNA levels are relative to the wild type strain. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

    Article Snippet: R. erythropolis strains were cultivated in LBP ( ) for conjugative transfer of DNA and in 7H9 minimal medium (Difco) supplemented with hexanoate at 6 mM (Sigma-Aldrich) as carbon source for induction assays.

    Techniques: Mutagenesis, Quantitative RT-PCR, Software, Quantitation Assay, Expressing

    Induction of the qsd operon expression by 3-oxo-C 8 -HSL. qRT-PCR analysis of the qsdA , qsdC , and qsdR transcription was conducted in the R. erythropolis R138 wild-type strain grown in 7H9 medium in which 3-oxo-C 8 -HSL was added at mid-exponential phase. Expression levels of qsdA , qsdC , and qsdR are relative to those obtained in the same medium without the inducer. The dotted line indicates the same mRNA expression between the induced condition and without the inducers. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

    Journal: Frontiers in Microbiology

    Article Title: A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response

    doi: 10.3389/fmicb.2018.02800

    Figure Lengend Snippet: Induction of the qsd operon expression by 3-oxo-C 8 -HSL. qRT-PCR analysis of the qsdA , qsdC , and qsdR transcription was conducted in the R. erythropolis R138 wild-type strain grown in 7H9 medium in which 3-oxo-C 8 -HSL was added at mid-exponential phase. Expression levels of qsdA , qsdC , and qsdR are relative to those obtained in the same medium without the inducer. The dotted line indicates the same mRNA expression between the induced condition and without the inducers. Data shown are mean values obtained from three independent experiments. Statistical analysis was performed by the DataAssist TM software (v3.01) used for calculating relative quantitation of gene expression, based on the comparative C T (2 -ΔΔCT ) method. ∗ p -value

    Article Snippet: R. erythropolis strains were cultivated in LBP ( ) for conjugative transfer of DNA and in 7H9 minimal medium (Difco) supplemented with hexanoate at 6 mM (Sigma-Aldrich) as carbon source for induction assays.

    Techniques: Expressing, Quantitative RT-PCR, Software, Quantitation Assay