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SPT Labtech stock solution
Stock Solution, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/7932/comPOUND/pm41290659-354-8-27
Average 93 stars, based on 13 article reviews

stock solution - by Bioz Stars, 2026-10

93/100 stars

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Cryo-EM Sample Prep:

Article Title: Inhibitors of NLRP3 inflammasome
Article Snippet: .. Cryo-EM Grid Preparation, Data Collection and Processing The purified NLRP3·Compound 007 complex was applied onto glow-discharged EM-grids (Quantifoil Cu300 R2/1+2 nm carbon support film), incubated for 30s at 100% humidity and 4° C., before it was blotted for 5s with blot force 5 and plunge frozen into liquid ethane using a Vitrobot mark IV plunge freezing device (Thermo Fisher Scientific). ..

Purification:

Incubation:

Article Title: A Tetrapodal Tryptophan Derivative with Multiple Exposed Free Carboxylic Acids Blocks Host Cell Entry of Omicron SARS-Cov-2 and Respiratory Syncytial Virus
Article Snippet: .. Prior to the addition of the compound, the spike protein was kept at 22 °C for 30 min, then the compound was added and the incubation with the compound was continued for 30 min before 3 μL aliquots were placed on cryo-EM grids (QUANTIFOIL R 1.2/1.3 Au:300-mesh grids) that had been made hydrophilic by glow discharge (30 s using a Leica EM ACE600 device). .. Prior to the addition of the compound, the spike protein was kept at 22 °C for 30 min, then the compound was added and the incubation with the compound was continued for 30 min before 3 μL aliquots were placed on cryo-EM grids (QUANTIFOIL R 1.2/1.3 Au:300-mesh grids) that had been made hydrophilic by glow discharge (30 s using a Leica EM ACE600 device).

Concentration Assay:

Article Title: Fragment-based discovery enables direct targeting of the melanoma oncogene MITF.
Article Snippet: The proteins were then diluted to a final concentration of 0.3 mg mL-1 in their respective storage buffers (for the wild-type: 50 mM HEPES (Sigma-Aldrich, USA), 300 mM NaCl (Sigma-Aldrich, USA), 5% (v/v) glycerol (PanReac AppliChem, Germany/Spain), 1 mM TCEP (SigmaAldrich, USA), pH 8 / for the variants: 25 mM HEPES (Sigma-Aldrich, USA), 300 mM NaCl (Sigma-Aldrich, USA), 10% (v/v) glycerol (PanReac AppliChem, Germany/Spain), 1 mM TCEP (Sigma-Aldrich, USA), pH 7.5). .. Compound 8, dissolved and diluted in DMSO (Sigma-Aldrich, USA) to a final concentration of 50 μM, was mixed with the wild-type protein (for the apo conditions, DMSO was used instead of the compound), or with buffer only, using a Mosquito HTS device (TTP LabTech, UK), resulting in a final DMSO concentration of 3% (v/v). ..

Article Title: Fragment-based discovery enables direct targeting of the melanoma oncogene MITF
Article Snippet: The proteins were then diluted to a final concentration of 0.3 mg mL -1 in their respective storage buffers (for the wild-type: 50 mM HEPES (Sigma-Aldrich, USA), 300 mM NaCl (Sigma-Aldrich, USA), 5% (v/v) glycerol (PanReac AppliChem, Germany/Spain), 1 mM TCEP (Sigma-Aldrich, USA), pH 8 / for the variants: 25 mM HEPES (Sigma-Aldrich, USA), 300 mM NaCl (Sigma-Aldrich, USA), 10% (v/v) glycerol (PanReac AppliChem, Germany/Spain), 1 mM TCEP (Sigma-Aldrich, USA), pH 7.5). .. Compound 8 , dissolved and diluted in DMSO (Sigma-Aldrich, USA) to a final concentration of 50 μM, was mixed with the wild-type protein (for the apo conditions, DMSO was used instead of the compound), or with buffer only, using a Mosquito HTS device (TTP LabTech, UK), resulting in a final DMSO concentration of 3% (v/v). .. The NMR data was processed with the software Topspin 3.6 (Bruker, Switzerland) and analyzed with CCPNMR 2.5 and 3.0 .

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