Structured Review

Bio-Rad qpcr analysis
Qpcr Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1822 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qpcr analysis/product/Bio-Rad
Average 95 stars, based on 1822 article reviews
Price from $9.99 to $1999.99
qpcr analysis - by Bioz Stars, 2020-01
95/100 stars

Images

Related Articles

Real-time Polymerase Chain Reaction:

Article Title: The fatty acid binding protein 6 gene (Fabp6) is expressed in murine granulosa cells and is involved in ovulatory response to superstimulation
Article Snippet: .. RNA and protein detection Total RNA was purified from ovaries using the TRIzol reagent (Life Technologies, Burlington, ON) and then converted to cDNA for qPCR analysis (Bio-Rad CFX384 system, Bio-Rad, Mississauga, ON). ..

Article Title: Bacterial inactivation by plasma treated water enhanced by reactive nitrogen species
Article Snippet: .. The resulting cDNA was used for the qPCR analysis (CFX96, Biorad) with primers (Macrogen) of 16 s rRNA (the RNA component of the small subunit used as house-keeping gene), OxyR, RpoE, GroES, and DnaK. .. DNA extraction for agarose gel electrophoresis Genomic DNA was extracted after 3 h exposure with PTW produced by N2, N2 + H2 O and N2 + 0.5 wt% HNO3 for 1% agarose analysis.

RNA Extraction:

Article Title: Bacterial inactivation by plasma treated water enhanced by reactive nitrogen species
Article Snippet: Paragraph title: RNA extraction for quantitative real time PCR ... The resulting cDNA was used for the qPCR analysis (CFX96, Biorad) with primers (Macrogen) of 16 s rRNA (the RNA component of the small subunit used as house-keeping gene), OxyR, RpoE, GroES, and DnaK.

Size-exclusion Chromatography:

Article Title: The fatty acid binding protein 6 gene (Fabp6) is expressed in murine granulosa cells and is involved in ovulatory response to superstimulation
Article Snippet: RNA and protein detection Total RNA was purified from ovaries using the TRIzol reagent (Life Technologies, Burlington, ON) and then converted to cDNA for qPCR analysis (Bio-Rad CFX384 system, Bio-Rad, Mississauga, ON). .. The thermal cycler profile used in the analyses is as follows: initial denaturation step of 5 min at 95 C, followed by 35 cycles of 15 sec at 95 C denaturation step and 30 sec at 58 C annealing/extension step.

Purification:

Article Title: The fatty acid binding protein 6 gene (Fabp6) is expressed in murine granulosa cells and is involved in ovulatory response to superstimulation
Article Snippet: .. RNA and protein detection Total RNA was purified from ovaries using the TRIzol reagent (Life Technologies, Burlington, ON) and then converted to cDNA for qPCR analysis (Bio-Rad CFX384 system, Bio-Rad, Mississauga, ON). ..

Produced:

Article Title: Bacterial inactivation by plasma treated water enhanced by reactive nitrogen species
Article Snippet: RNA extraction for quantitative real time PCR To perform a quantitative evaluation of oxidative related gene expression, after 3 h exposure with PTW produced by N2 , N2 + H2 O and N2 + 0.5 wt% HNO3 , the total RNA was extracted from treated and untreated samples of E. coli using an RNeasy Mini Kit, and it was converted to cDNA using reverse transcriptase and random primers (GoScriptTM Reverse Transcription System, Promega). .. The resulting cDNA was used for the qPCR analysis (CFX96, Biorad) with primers (Macrogen) of 16 s rRNA (the RNA component of the small subunit used as house-keeping gene), OxyR, RpoE, GroES, and DnaK.

Expressing:

Article Title: Bacterial inactivation by plasma treated water enhanced by reactive nitrogen species
Article Snippet: RNA extraction for quantitative real time PCR To perform a quantitative evaluation of oxidative related gene expression, after 3 h exposure with PTW produced by N2 , N2 + H2 O and N2 + 0.5 wt% HNO3 , the total RNA was extracted from treated and untreated samples of E. coli using an RNeasy Mini Kit, and it was converted to cDNA using reverse transcriptase and random primers (GoScriptTM Reverse Transcription System, Promega). .. The resulting cDNA was used for the qPCR analysis (CFX96, Biorad) with primers (Macrogen) of 16 s rRNA (the RNA component of the small subunit used as house-keeping gene), OxyR, RpoE, GroES, and DnaK.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Bio-Rad cfx96 touch real time pcr detection system
    Cfx96 Touch Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 2066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfx96 touch real time pcr detection system/product/Bio-Rad
    Average 90 stars, based on 2066 article reviews
    Price from $9.99 to $1999.99
    cfx96 touch real time pcr detection system - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    88
    Bio-Rad abi prism 7900ht
    Thermal variability upon amplification of 18s rRNA using 5 ng/µl human genomic DNA. (A) CFX96, (B) xxpress®, (C) <t>ABI</t> Prism <t>7900HT</t> and (D) Rotor-Gene Q.
    Abi Prism 7900ht, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi prism 7900ht/product/Bio-Rad
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    abi prism 7900ht - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    Image Search Results


    Thermal variability upon amplification of 18s rRNA using 5 ng/µl human genomic DNA. (A) CFX96, (B) xxpress®, (C) ABI Prism 7900HT and (D) Rotor-Gene Q.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives

    doi: 10.3892/etm.2015.2712

    Figure Lengend Snippet: Thermal variability upon amplification of 18s rRNA using 5 ng/µl human genomic DNA. (A) CFX96, (B) xxpress®, (C) ABI Prism 7900HT and (D) Rotor-Gene Q.

    Article Snippet: Thermal variability was assessed in qPCR by measuring the amplification of 18S rRNA in a selection of wells covering all areas of the sample plate on ABI Prism 7900HT, Bio-Rad CFX96 System, Qiagen Rotor-Gene Q and BJS Biotechnologies xxpress instruments.

    Techniques: Amplification