7900ht quantitative polymerase chain reaction pcr instrument  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 7900ht quantitative polymerase chain reaction pcr instrument
    7900ht Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7900ht quantitative polymerase chain reaction pcr instrument/product/Thermo Fisher
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    7900ht quantitative polymerase chain reaction pcr instrument - by Bioz Stars, 2020-09
    91/100 stars

    Related Products / Commonly Used Together

    cdna
    taqman gene expression assays
    mrna quantitation

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    Real-time Polymerase Chain Reaction:

    Article Title: An Investigation of the Single and Combined Phthalate Metabolite Effects on Human Chorionic Gonadotropin Expression in Placental Cells
    Article Snippet: .. TaqMan™ gene expression assays (see Table S4) were used for mRNA quantitation, and 10 ng of cDNA was loaded in a 10 - μ L reaction volume and run in triplicate on a 7900HT quantitative polymerase chain reaction (PCR) instrument (Applied Biosystems). ..

    Article Title: An Investigation of the Single and Combined Phthalate Metabolite Effects on Human Chorionic Gonadotropin Expression in Placental Cells
    Article Snippet: .. TaqMan™ gene expression assays (see Table S4) were used for mRNA quantitation, and 10 ng of cDNA was loaded in a 10 - μL reaction volume and run in triplicate on a 7900HT quantitative polymerase chain reaction (PCR) instrument (Applied Biosystems). ..

    Polymerase Chain Reaction:

    Article Title: An Investigation of the Single and Combined Phthalate Metabolite Effects on Human Chorionic Gonadotropin Expression in Placental Cells
    Article Snippet: .. TaqMan™ gene expression assays (see Table S4) were used for mRNA quantitation, and 10 ng of cDNA was loaded in a 10 - μ L reaction volume and run in triplicate on a 7900HT quantitative polymerase chain reaction (PCR) instrument (Applied Biosystems). ..

    Article Title: An Investigation of the Single and Combined Phthalate Metabolite Effects on Human Chorionic Gonadotropin Expression in Placental Cells
    Article Snippet: .. TaqMan™ gene expression assays (see Table S4) were used for mRNA quantitation, and 10 ng of cDNA was loaded in a 10 - μL reaction volume and run in triplicate on a 7900HT quantitative polymerase chain reaction (PCR) instrument (Applied Biosystems). ..

    Quantitation Assay:

    Article Title: An Investigation of the Single and Combined Phthalate Metabolite Effects on Human Chorionic Gonadotropin Expression in Placental Cells
    Article Snippet: .. TaqMan™ gene expression assays (see Table S4) were used for mRNA quantitation, and 10 ng of cDNA was loaded in a 10 - μ L reaction volume and run in triplicate on a 7900HT quantitative polymerase chain reaction (PCR) instrument (Applied Biosystems). ..

    Article Title: An Investigation of the Single and Combined Phthalate Metabolite Effects on Human Chorionic Gonadotropin Expression in Placental Cells
    Article Snippet: .. TaqMan™ gene expression assays (see Table S4) were used for mRNA quantitation, and 10 ng of cDNA was loaded in a 10 - μL reaction volume and run in triplicate on a 7900HT quantitative polymerase chain reaction (PCR) instrument (Applied Biosystems). ..

    Expressing:

    Article Title: An Investigation of the Single and Combined Phthalate Metabolite Effects on Human Chorionic Gonadotropin Expression in Placental Cells
    Article Snippet: .. TaqMan™ gene expression assays (see Table S4) were used for mRNA quantitation, and 10 ng of cDNA was loaded in a 10 - μ L reaction volume and run in triplicate on a 7900HT quantitative polymerase chain reaction (PCR) instrument (Applied Biosystems). ..

    Article Title: An Investigation of the Single and Combined Phthalate Metabolite Effects on Human Chorionic Gonadotropin Expression in Placental Cells
    Article Snippet: .. TaqMan™ gene expression assays (see Table S4) were used for mRNA quantitation, and 10 ng of cDNA was loaded in a 10 - μL reaction volume and run in triplicate on a 7900HT quantitative polymerase chain reaction (PCR) instrument (Applied Biosystems). ..

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    Thermo Fisher real time pcr rt qpcr
    The hAgo2-miRNA complex transferred by MPs into Plasmodium parasite in vitro and in vivo. ( A ) Annotations of high-throughput sequencing data of small RNAs bound by hAgo2. The read counts of each RNA class are listed in round brackets. ( B ) Fluorescence in situ hybridization detection of miR-451 in iRBCs. Black arrows in light fields indicate the parasites. miR-451 was labeled with 5′ carboxyfluorescein (FAM)/scramble probes (green), and the parasite nuclei were labeled with DAPI (blue). ( C ) <t>RT-qPCR</t> (left top) and stem-loop <t>RT-PCR</t> (left bottom) analysis of miR-451, miR-486 and miR-181a within nMPs and iMPs; RT-qPCR (right top) and northern blot (right bottom) analysis of these miRNAs in nRBCs and iRBCs. ( D ) RT-qPCR analysis of miR-451 in iRBCs treated with nMPs (left) and iMPs (right). Synchronized ring stage iRBCs were incubated with different concentrations of nMPs (1 ×, 10 ×, 100 ×) or iMPs at separate times (16, 32 h). The level of miR-451 in iRBCs/nRBCs was given as a relative value of 1.0 ( n =3). ( E ) RT-qPCR analysis of Mmu-miR-451 in the nRBCs (Mmu-nRBCs) and iRBCs of P. yoelii -infected mice ( P.y. iRBCs) at different parasitemia ratios (1%±0.5% and 10%±5%) (i.e., three mice in each group) ( n =3).
    Real Time Pcr Rt Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr rt qpcr/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
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    99
    Thermo Fisher real time qpcr analysis
    Depletion of z-miR-200- class in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), or injected with control MO, or injected with anti- z-miR-200 class (right panels) MO. The control MO did not cause significant alterations. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing normal embryo morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Proportions of embryos showing either placode disorganization, olfactory axon mistargeting, or both (last bars) after injection of control or z-miR-200 class MOs. e. Quantification of endogenous miR-200 class in embryos injected with control or anti- z-miR-200 MOs, by Real-Time <t>qPCR.</t> Results show a significant decrease of miR-200 abundance in the injected embryos. f. Quantification of endogenous z-foxg1 <t>mRNA</t> in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-200 causes a significant increase of z-foxg1 mRNA abundance. g. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-200 class MOs, relative to control MO injected embryos, set = 1. The abundance of z-hoxA7a and abundance of z-hoxA10b were also determined and used as in Fig. 5 .
    Real Time Qpcr Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time qpcr analysis/product/Thermo Fisher
    Average 99 stars, based on 3966 article reviews
    Price from $9.99 to $1999.99
    real time qpcr analysis - by Bioz Stars, 2020-09
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    The hAgo2-miRNA complex transferred by MPs into Plasmodium parasite in vitro and in vivo. ( A ) Annotations of high-throughput sequencing data of small RNAs bound by hAgo2. The read counts of each RNA class are listed in round brackets. ( B ) Fluorescence in situ hybridization detection of miR-451 in iRBCs. Black arrows in light fields indicate the parasites. miR-451 was labeled with 5′ carboxyfluorescein (FAM)/scramble probes (green), and the parasite nuclei were labeled with DAPI (blue). ( C ) RT-qPCR (left top) and stem-loop RT-PCR (left bottom) analysis of miR-451, miR-486 and miR-181a within nMPs and iMPs; RT-qPCR (right top) and northern blot (right bottom) analysis of these miRNAs in nRBCs and iRBCs. ( D ) RT-qPCR analysis of miR-451 in iRBCs treated with nMPs (left) and iMPs (right). Synchronized ring stage iRBCs were incubated with different concentrations of nMPs (1 ×, 10 ×, 100 ×) or iMPs at separate times (16, 32 h). The level of miR-451 in iRBCs/nRBCs was given as a relative value of 1.0 ( n =3). ( E ) RT-qPCR analysis of Mmu-miR-451 in the nRBCs (Mmu-nRBCs) and iRBCs of P. yoelii -infected mice ( P.y. iRBCs) at different parasitemia ratios (1%±0.5% and 10%±5%) (i.e., three mice in each group) ( n =3).

    Journal: Emerging Microbes & Infections

    Article Title: Red blood cells release microparticles containing human argonaute 2 and miRNAs to target genes of Plasmodium falciparum

    doi: 10.1038/emi.2017.63

    Figure Lengend Snippet: The hAgo2-miRNA complex transferred by MPs into Plasmodium parasite in vitro and in vivo. ( A ) Annotations of high-throughput sequencing data of small RNAs bound by hAgo2. The read counts of each RNA class are listed in round brackets. ( B ) Fluorescence in situ hybridization detection of miR-451 in iRBCs. Black arrows in light fields indicate the parasites. miR-451 was labeled with 5′ carboxyfluorescein (FAM)/scramble probes (green), and the parasite nuclei were labeled with DAPI (blue). ( C ) RT-qPCR (left top) and stem-loop RT-PCR (left bottom) analysis of miR-451, miR-486 and miR-181a within nMPs and iMPs; RT-qPCR (right top) and northern blot (right bottom) analysis of these miRNAs in nRBCs and iRBCs. ( D ) RT-qPCR analysis of miR-451 in iRBCs treated with nMPs (left) and iMPs (right). Synchronized ring stage iRBCs were incubated with different concentrations of nMPs (1 ×, 10 ×, 100 ×) or iMPs at separate times (16, 32 h). The level of miR-451 in iRBCs/nRBCs was given as a relative value of 1.0 ( n =3). ( E ) RT-qPCR analysis of Mmu-miR-451 in the nRBCs (Mmu-nRBCs) and iRBCs of P. yoelii -infected mice ( P.y. iRBCs) at different parasitemia ratios (1%±0.5% and 10%±5%) (i.e., three mice in each group) ( n =3).

    Article Snippet: For detection of miRNAs by quantitative real-time PCR (RT-qPCR), synthesized Arabidopsis thaliana miR-404 RNA oligos (ath-miR-404) were added to counted cells (2 nmol/108 RBCs) before total RNA extraction and were used as an exogenous reference for data normalization.

    Techniques: In Vitro, In Vivo, Next-Generation Sequencing, Fluorescence, In Situ Hybridization, Labeling, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Northern Blot, Incubation, Infection, Mouse Assay

    Depletion of z-miR-200- class in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), or injected with control MO, or injected with anti- z-miR-200 class (right panels) MO. The control MO did not cause significant alterations. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing normal embryo morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Proportions of embryos showing either placode disorganization, olfactory axon mistargeting, or both (last bars) after injection of control or z-miR-200 class MOs. e. Quantification of endogenous miR-200 class in embryos injected with control or anti- z-miR-200 MOs, by Real-Time qPCR. Results show a significant decrease of miR-200 abundance in the injected embryos. f. Quantification of endogenous z-foxg1 mRNA in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-200 causes a significant increase of z-foxg1 mRNA abundance. g. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-200 class MOs, relative to control MO injected embryos, set = 1. The abundance of z-hoxA7a and abundance of z-hoxA10b were also determined and used as in Fig. 5 .

    Journal: Molecular and Cellular Neurosciences

    Article Title: The Dlx5 and Foxg1 transcription factors, linked via miRNA-9 and -200, are required for the development of the olfactory and GnRH system

    doi: 10.1016/j.mcn.2015.04.007

    Figure Lengend Snippet: Depletion of z-miR-200- class in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), or injected with control MO, or injected with anti- z-miR-200 class (right panels) MO. The control MO did not cause significant alterations. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing normal embryo morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Proportions of embryos showing either placode disorganization, olfactory axon mistargeting, or both (last bars) after injection of control or z-miR-200 class MOs. e. Quantification of endogenous miR-200 class in embryos injected with control or anti- z-miR-200 MOs, by Real-Time qPCR. Results show a significant decrease of miR-200 abundance in the injected embryos. f. Quantification of endogenous z-foxg1 mRNA in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-200 causes a significant increase of z-foxg1 mRNA abundance. g. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-200 class MOs, relative to control MO injected embryos, set = 1. The abundance of z-hoxA7a and abundance of z-hoxA10b were also determined and used as in Fig. 5 .

    Article Snippet: 2.7 Real-Time qPCR analysis for coding mRNAs Relative mRNA abundance was determined by Real-Time qPCR.

    Techniques: Fluorescence, Injection, Real-time Polymerase Chain Reaction

    Depletion of z-miR-9 in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos injected with control (top panels) or anti- z-miR-9 (bottom panels) MOs. The control MO did not cause any significant alteration. Arrows indicate the normal axonal pathway in the control embryos. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing a normal embryonic morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Quantification of endogenous miR-9 in zebrafish embryos injected with control or anti- z-miR-9 MOs, by Real-Time qPCR. Results show efficient depletion. e. Quantification of endogenous z-foxg1 mRNAs in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-9 causes a significant increase of the z-foxg1 mRNA. f. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-9 MOs, relative to control MO injected embryos. Samples were collected 72 hpf. Results are shown relative to the control injected samples, made = 1. The relative abundance of z-hoxA7a and relative abundance of z-hoxA10b mRNAs were determined, to monitor progression of development and exclude a generalized delay.

    Journal: Molecular and Cellular Neurosciences

    Article Title: The Dlx5 and Foxg1 transcription factors, linked via miRNA-9 and -200, are required for the development of the olfactory and GnRH system

    doi: 10.1016/j.mcn.2015.04.007

    Figure Lengend Snippet: Depletion of z-miR-9 in zebrafish embryos causes delayed olfactory differentiation. a. Micrographs of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos injected with control (top panels) or anti- z-miR-9 (bottom panels) MOs. The control MO did not cause any significant alteration. Arrows indicate the normal axonal pathway in the control embryos. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing a normal embryonic morphology and growth rate. c. Proportion of embryos showing either YFP or CFP fluorescence, upon injection of control or z-dlx5a MO. A significant loss of CFP + embryos is detected. d. Quantification of endogenous miR-9 in zebrafish embryos injected with control or anti- z-miR-9 MOs, by Real-Time qPCR. Results show efficient depletion. e. Quantification of endogenous z-foxg1 mRNAs in zebrafish embryos injected with anti- z-miR-9 MO, by Real-Time qPCR. Results show that depletion of miR-9 causes a significant increase of the z-foxg1 mRNA. f. Quantification of developmental markers, by Real-Time pPCR, in zebrafish embryos injected with anti- miR-9 MOs, relative to control MO injected embryos. Samples were collected 72 hpf. Results are shown relative to the control injected samples, made = 1. The relative abundance of z-hoxA7a and relative abundance of z-hoxA10b mRNAs were determined, to monitor progression of development and exclude a generalized delay.

    Article Snippet: 2.7 Real-Time qPCR analysis for coding mRNAs Relative mRNA abundance was determined by Real-Time qPCR.

    Techniques: Fluorescence, Injection, Real-time Polymerase Chain Reaction

    Depletion of z-dlx5a in zebrafish embryos causes delayed olfactory differentiation. a. Confocal stacked images of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), injected with a control MO (right), or injected with anti- z-dlx5a MO (bottom panels), taken at 72 hpf. Arrows indicate the normal axonal pathway in the control embryos. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing an overall normal embryonic morphology and growth rate in the injected embryos, compared to the non-injected ones. c. Percentages of embryos showing YFP or CFP fluorescence, over the total of examined ones, comparing not-injected, control injected and MO injected ones. d. RT-PCR analysis on RNA extracted from anti- z-dlx5a MO-treated and control embryos, showing that the MO efficiently generates an inactive splice-variant form of the endogenous mRNA. A scheme of the z-dlx5a gene (Ex1–Ex2–Ex3), the positions of the z-dlx5a MO and the position of the PCR primers (A–D) are reported on the left. e. (on the left) Quantification of the olfactory differentiation phenotype by Real-Time qPCR for differentiation-related mRNAs in a sample of the embryonic heads of MO-injected embryos (grey bars). Embryos injected with control MO were used for comparison (open bars). Normalization is carried out relative to control samples, made = 1. (on the right) Relative abundance of z-hoxA7a and relative abundance of z-hoxA10b mRNAs in whole embryos injected with z-dlx5a MO (grey bars), to monitor developmental progression and exclude a generalized delay.

    Journal: Molecular and Cellular Neurosciences

    Article Title: The Dlx5 and Foxg1 transcription factors, linked via miRNA-9 and -200, are required for the development of the olfactory and GnRH system

    doi: 10.1016/j.mcn.2015.04.007

    Figure Lengend Snippet: Depletion of z-dlx5a in zebrafish embryos causes delayed olfactory differentiation. a. Confocal stacked images of Trcp2::Venus (left, yellow fluorescence) and OMP::CFP (right, cyan fluorescence) zebrafish embryos not injected (left), injected with a control MO (right), or injected with anti- z-dlx5a MO (bottom panels), taken at 72 hpf. Arrows indicate the normal axonal pathway in the control embryos. Asterisks indicate the regions of reduced fluorescence intensity. b. Whole-mount bright field micrographs of injected embryo, showing an overall normal embryonic morphology and growth rate in the injected embryos, compared to the non-injected ones. c. Percentages of embryos showing YFP or CFP fluorescence, over the total of examined ones, comparing not-injected, control injected and MO injected ones. d. RT-PCR analysis on RNA extracted from anti- z-dlx5a MO-treated and control embryos, showing that the MO efficiently generates an inactive splice-variant form of the endogenous mRNA. A scheme of the z-dlx5a gene (Ex1–Ex2–Ex3), the positions of the z-dlx5a MO and the position of the PCR primers (A–D) are reported on the left. e. (on the left) Quantification of the olfactory differentiation phenotype by Real-Time qPCR for differentiation-related mRNAs in a sample of the embryonic heads of MO-injected embryos (grey bars). Embryos injected with control MO were used for comparison (open bars). Normalization is carried out relative to control samples, made = 1. (on the right) Relative abundance of z-hoxA7a and relative abundance of z-hoxA10b mRNAs in whole embryos injected with z-dlx5a MO (grey bars), to monitor developmental progression and exclude a generalized delay.

    Article Snippet: 2.7 Real-Time qPCR analysis for coding mRNAs Relative mRNA abundance was determined by Real-Time qPCR.

    Techniques: Fluorescence, Injection, Reverse Transcription Polymerase Chain Reaction, Variant Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    miR-24 negatively regulates menin. A: Real-time PCR evaluation of miR-24 expression in CCA and H69 cell lines demonstrates increased levels in CCA lines compared to H69 cells. B: Luciferase luminescence shows decreased menin expression with miR-24 mimic treatment. C: Left panel: miRNA-sequence data demonstrate increased expression of miR-24 in human CCA tumors compared with matched normal tissue. Right panel: Statistical significance of increased miR-24 expression is validated with an unpaired t -test. Data are expressed as means ± SEM ( A–C ). n = 3 ( A and B ); n = 9 ( C ). ∗ P

    Journal: The American Journal of Pathology

    Article Title: miR-24 Inhibition Increases Menin Expression and Decreases Cholangiocarcinoma Proliferation

    doi: 10.1016/j.ajpath.2016.10.021

    Figure Lengend Snippet: miR-24 negatively regulates menin. A: Real-time PCR evaluation of miR-24 expression in CCA and H69 cell lines demonstrates increased levels in CCA lines compared to H69 cells. B: Luciferase luminescence shows decreased menin expression with miR-24 mimic treatment. C: Left panel: miRNA-sequence data demonstrate increased expression of miR-24 in human CCA tumors compared with matched normal tissue. Right panel: Statistical significance of increased miR-24 expression is validated with an unpaired t -test. Data are expressed as means ± SEM ( A–C ). n = 3 ( A and B ); n = 9 ( C ). ∗ P

    Article Snippet: Transfection efficacy was assessed by measuring menin expression by real-time quantitative PCR and flow cytometry., Cell proliferation was measured by Ki-67 real-time PCR expression, migration via wound healing, invasion via Boyden chamber, and angiogenesis via VEGF-A, VEGF-C, VEGFR-2, VEGFR-3, ANG-1, ANG-2, TIE-1, and TIE-2 real-time PCR expression.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Luciferase, Sequencing

    Increased menin expression decreases proliferation. Mz-ChA-1 cells overexpressing menin with pCMV6-MEN1 vector exhibit a decrease in Ki-67 proliferative marker expression. A – C: Increased menin expression in pCMV6-MEN1 Mz-ChA-1 cells by real-time PCR ( A ) and flow cytometry ( B ) decreased Ki-67 proliferative marker expression by real-time PCR ( C ). D: Decreased cell migration as measured by wound healing assay. E: Decreased cell invasion as measured by Boyden chamber assay in pCMV6-MEN1 Mz-ChA-1 cells. Data are expressed as means ± SEM performed in triplicate ( A–E ). ∗ P

    Journal: The American Journal of Pathology

    Article Title: miR-24 Inhibition Increases Menin Expression and Decreases Cholangiocarcinoma Proliferation

    doi: 10.1016/j.ajpath.2016.10.021

    Figure Lengend Snippet: Increased menin expression decreases proliferation. Mz-ChA-1 cells overexpressing menin with pCMV6-MEN1 vector exhibit a decrease in Ki-67 proliferative marker expression. A – C: Increased menin expression in pCMV6-MEN1 Mz-ChA-1 cells by real-time PCR ( A ) and flow cytometry ( B ) decreased Ki-67 proliferative marker expression by real-time PCR ( C ). D: Decreased cell migration as measured by wound healing assay. E: Decreased cell invasion as measured by Boyden chamber assay in pCMV6-MEN1 Mz-ChA-1 cells. Data are expressed as means ± SEM performed in triplicate ( A–E ). ∗ P

    Article Snippet: Transfection efficacy was assessed by measuring menin expression by real-time quantitative PCR and flow cytometry., Cell proliferation was measured by Ki-67 real-time PCR expression, migration via wound healing, invasion via Boyden chamber, and angiogenesis via VEGF-A, VEGF-C, VEGFR-2, VEGFR-3, ANG-1, ANG-2, TIE-1, and TIE-2 real-time PCR expression.

    Techniques: Expressing, Plasmid Preparation, Marker, Real-time Polymerase Chain Reaction, Flow Cytometry, Migration, Wound Healing Assay, Boyden Chamber Assay

    Menin expression negatively regulates angiogenesis. A: By real-time PCR, Mz-ChA-1 MEN1 knockout cells increased expression of angiogenic factors compared to Mz-ChA-1 control cells. B: By real-time PCR, pCMV6-MEN1 Mz-ChA-1 cells decreased expression of angiogenic factors compared to Mz-ChA-1 control cells. Data are expressed as means ± SEM performed in triplicate ( A and B ). ∗ P

    Journal: The American Journal of Pathology

    Article Title: miR-24 Inhibition Increases Menin Expression and Decreases Cholangiocarcinoma Proliferation

    doi: 10.1016/j.ajpath.2016.10.021

    Figure Lengend Snippet: Menin expression negatively regulates angiogenesis. A: By real-time PCR, Mz-ChA-1 MEN1 knockout cells increased expression of angiogenic factors compared to Mz-ChA-1 control cells. B: By real-time PCR, pCMV6-MEN1 Mz-ChA-1 cells decreased expression of angiogenic factors compared to Mz-ChA-1 control cells. Data are expressed as means ± SEM performed in triplicate ( A and B ). ∗ P

    Article Snippet: Transfection efficacy was assessed by measuring menin expression by real-time quantitative PCR and flow cytometry., Cell proliferation was measured by Ki-67 real-time PCR expression, migration via wound healing, invasion via Boyden chamber, and angiogenesis via VEGF-A, VEGF-C, VEGFR-2, VEGFR-3, ANG-1, ANG-2, TIE-1, and TIE-2 real-time PCR expression.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Knock-Out

    Menin is down-regulated in CCA. A and B: By real-time PCR and immunoblots, menin expression is decreased in CCA cell lines compared to H69. Significance is shown versus H69 cells. C: Flow cytometry analysis demonstrated a decrease in menin protein expression in Mz-ChA-1 cells compared to H69 cells. D: By real-time PCR, menin expression decreased in advanced-stage human CCA tissue biopsy specimens compared with normal control. Data are expressed as means ± SEM performed in triplicate ( A – D ). n = 3 independent samples ( A and B ). ∗ P

    Journal: The American Journal of Pathology

    Article Title: miR-24 Inhibition Increases Menin Expression and Decreases Cholangiocarcinoma Proliferation

    doi: 10.1016/j.ajpath.2016.10.021

    Figure Lengend Snippet: Menin is down-regulated in CCA. A and B: By real-time PCR and immunoblots, menin expression is decreased in CCA cell lines compared to H69. Significance is shown versus H69 cells. C: Flow cytometry analysis demonstrated a decrease in menin protein expression in Mz-ChA-1 cells compared to H69 cells. D: By real-time PCR, menin expression decreased in advanced-stage human CCA tissue biopsy specimens compared with normal control. Data are expressed as means ± SEM performed in triplicate ( A – D ). n = 3 independent samples ( A and B ). ∗ P

    Article Snippet: Transfection efficacy was assessed by measuring menin expression by real-time quantitative PCR and flow cytometry., Cell proliferation was measured by Ki-67 real-time PCR expression, migration via wound healing, invasion via Boyden chamber, and angiogenesis via VEGF-A, VEGF-C, VEGFR-2, VEGFR-3, ANG-1, ANG-2, TIE-1, and TIE-2 real-time PCR expression.

    Techniques: Real-time Polymerase Chain Reaction, Western Blot, Expressing, Flow Cytometry

    miR-24 drives proliferation. A: Real-time PCR confirmed knockdown of miR-24 in Mz-ChA-1 cells by hairpin inhibitor. miR-24 knockdown increased menin expression via fluorescence-activated cell sorting ( B ) and decreased expression of angiogenic factors via real-time PCR ( C ). Data are expressed as means ± SEM performed in triplicate unless otherwise stated ( A – C ). ∗ P

    Journal: The American Journal of Pathology

    Article Title: miR-24 Inhibition Increases Menin Expression and Decreases Cholangiocarcinoma Proliferation

    doi: 10.1016/j.ajpath.2016.10.021

    Figure Lengend Snippet: miR-24 drives proliferation. A: Real-time PCR confirmed knockdown of miR-24 in Mz-ChA-1 cells by hairpin inhibitor. miR-24 knockdown increased menin expression via fluorescence-activated cell sorting ( B ) and decreased expression of angiogenic factors via real-time PCR ( C ). Data are expressed as means ± SEM performed in triplicate unless otherwise stated ( A – C ). ∗ P

    Article Snippet: Transfection efficacy was assessed by measuring menin expression by real-time quantitative PCR and flow cytometry., Cell proliferation was measured by Ki-67 real-time PCR expression, migration via wound healing, invasion via Boyden chamber, and angiogenesis via VEGF-A, VEGF-C, VEGFR-2, VEGFR-3, ANG-1, ANG-2, TIE-1, and TIE-2 real-time PCR expression.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Fluorescence, FACS

    Comprehensive study of oncogenic miRNAs in T-ALL. ( a ) Schematic of the experimental strategy. ( b ) Average miRNA expression across 50 T-ALL samples by quantitative RT-PCR and normalized to the mean expression value of all expressed miRNAs in a given sample

    Journal: Nature genetics

    Article Title: A cooperative microRNA-tumor suppressor gene network in acute T-cell lymphoblastic leukemia (T-ALL)

    doi: 10.1038/ng.858

    Figure Lengend Snippet: Comprehensive study of oncogenic miRNAs in T-ALL. ( a ) Schematic of the experimental strategy. ( b ) Average miRNA expression across 50 T-ALL samples by quantitative RT-PCR and normalized to the mean expression value of all expressed miRNAs in a given sample

    Article Snippet: Real time quantitative PCR for gene expression Total RNA and miRNA-enriched RNA was extracted using the AllPrep DNA/RNA/Protein and miRNeasy Mini. cDNA synthesis and qRT-PCR and analysis by the ΔΔ Ct method was performed as described .

    Techniques: Expressing, Quantitative RT-PCR