e coli jm109  (ATCC)

Journal: BMC Microbiology

Article Title: High-throughput metal susceptibility testing of microbial biofilms

doi: 10.1186/1471-2180-5-53

Figure Lengend Snippet: Growth of bacterial biofilms in the MBEC assay . (A) Mean cell density of Pseudomonas aeruginosa ATCC 27853 biofilms on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.842 using one-way ANOVA). (B) SEM photomicrograph of a P. aeruginosa biofilm on the peg surface. (C) Mean cell density of Escherichia coli TG1 on the pegs in different rows of the MBEC assay. Each value is expressed as the mean and standard deviation of 4 to 6 trials. There is no significant difference between cell density of biofilms in the different rows ( p = 0.274 using one-way ANOVA). (D) SEM photomicrograph of an E. coli biofilm on the peg surface. The bar represents 5 μm.

Article Snippet: Escherichia coli TG1, E. coli JM109 and Pseudomonas aeruginosa ATCC 27853 were stored at -70°C in Cryobanks™ (Prolab Diagnostics) according to the manufacturer's instructions.

Techniques: Standard Deviation

Journal: BMC Microbiology

Article Title: High-throughput metal susceptibility testing of microbial biofilms

doi: 10.1186/1471-2180-5-53

Figure Lengend Snippet: Susceptibility of Escherichia coli JM109 to metal cations with 2 or 24 h of exposure in rich (LB + B1) medium

Article Snippet: Escherichia coli TG1, E. coli JM109 and Pseudomonas aeruginosa ATCC 27853 were stored at -70°C in Cryobanks™ (Prolab Diagnostics) according to the manufacturer's instructions.

Techniques:

Journal: BMC Microbiology

Article Title: High-throughput metal susceptibility testing of microbial biofilms

doi: 10.1186/1471-2180-5-53

Figure Lengend Snippet: Susceptibility of Escherichia coli JM109 to metal cations with 2 or 24 h of exposure in minimal (MSVG) medium

Article Snippet: Escherichia coli TG1, E. coli JM109 and Pseudomonas aeruginosa ATCC 27853 were stored at -70°C in Cryobanks™ (Prolab Diagnostics) according to the manufacturer's instructions.

Techniques:

Journal: BMC Microbiology

Article Title: High-throughput metal susceptibility testing of microbial biofilms

doi: 10.1186/1471-2180-5-53

Figure Lengend Snippet: Potential neutralizing agents for the microbiological application of inactivating metals cations and oxyanions*

Article Snippet: Escherichia coli TG1, E. coli JM109 and Pseudomonas aeruginosa ATCC 27853 were stored at -70°C in Cryobanks™ (Prolab Diagnostics) according to the manufacturer's instructions.

Techniques:

Journal: Cancer Management and Research

Article Title: Knockdown of NUDT21 inhibits proliferation and promotes apoptosis of human K562 leukemia cells through ERK pathway

doi: 10.2147/CMAR.S173496

Figure Lengend Snippet: Expression levels of NUDT21 in human leukemia. Notes: ( A ) mRNA expression levels of NUDT21 in bone marrow samples from healthy control subjects (HC1–HC15) and CML patients (P1–P15). The mRNA ( B ) expression levels of NUDT21 were evaluated by qRT-PCR in human normal PBMCs, human leukemia cells K562, Jurkat, and hl-60 cells. The protein ( C and D ) expression levels of NUDT21 were evaluated by Western blotting in human normal PBMCs, human leukemia cells K562, Jurkat, and hl-60 cells. GAPDH was used as internal control; the data are the mean ± SD for duplicate experiments. *** P < 0.001. Abbreviations: CML, chronic myelocytic leukemia; HC, healthy control; qRT-PCR, quantitative reverse polymerase chain reaction.

Article Snippet: Specific shRNA plasmid against NUDT21 targeting 5′-ACCTCCTCAGTATCCATAT-3′ and a non-silencing shRNA control plasmid targeting 5′-TTCTCCGAACGT-GTCACGT-3′ were synthesized by Santa Cruz Biotechnology Inc.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Polymerase Chain Reaction

Journal: Cancer Management and Research

Article Title: Knockdown of NUDT21 inhibits proliferation and promotes apoptosis of human K562 leukemia cells through ERK pathway

doi: 10.2147/CMAR.S173496

Figure Lengend Snippet: Knockdown of NUDT21 in leukemia cells. Notes: Western blotting ( A and B ) and qPCR ( C ) were used to confirm the knockdown effects of shRNAs against NUDT21 in K562, Jurkat and HL-60 cells. The data are the mean ± SD for duplicate experiments; *** P <0.001. Abbreviation: qRT-PCR, quantitative reverse polymerase chain reaction.

Article Snippet: Specific shRNA plasmid against NUDT21 targeting 5′-ACCTCCTCAGTATCCATAT-3′ and a non-silencing shRNA control plasmid targeting 5′-TTCTCCGAACGT-GTCACGT-3′ were synthesized by Santa Cruz Biotechnology Inc.

Techniques: Western Blot, Quantitative RT-PCR, Polymerase Chain Reaction

Journal: Cancer Management and Research

Article Title: Knockdown of NUDT21 inhibits proliferation and promotes apoptosis of human K562 leukemia cells through ERK pathway

doi: 10.2147/CMAR.S173496

Figure Lengend Snippet: K562 cell growth upon knockdown of NUDT21. Notes: ( A ) Proliferation capabilities were detected by CCK-8 in K562, Jurkat, and HL-60 cells (2×10 3 ) for 5 days after transfection of shRNA control or NUDT21 shRNA. ( B ) K562 cells were transfected with shRNA control or NUDT21 shRNA for 48 hours and re-seeded in 96-well plate for BrdU assays. ( C ) Cell cycle phase distributions were detected by flow cytometry in K562 cells after transfection of shRNA control or NUDT21 shRNA. ( D ) Western blotting analysis of the protein expressions of PCNA and cyclin E after transfection of shRNA control or NUDT21 shRNA. The data are expressed as mean ± SD for duplicate experiments. * P <0.05; ** P <0.01; *** P <0.001. Abbreviations: CCK-8, Cell Counting Kit-8; BrdU, bromodeoxyuridine.

Article Snippet: Specific shRNA plasmid against NUDT21 targeting 5′-ACCTCCTCAGTATCCATAT-3′ and a non-silencing shRNA control plasmid targeting 5′-TTCTCCGAACGT-GTCACGT-3′ were synthesized by Santa Cruz Biotechnology Inc.

Techniques: CCK-8 Assay, Transfection, shRNA, Flow Cytometry, Western Blot, Cell Counting

Journal: Cancer Management and Research

Article Title: Knockdown of NUDT21 inhibits proliferation and promotes apoptosis of human K562 leukemia cells through ERK pathway

doi: 10.2147/CMAR.S173496

Figure Lengend Snippet: K562 cell apoptosis upon knockdown of NUDT21. Notes: ( A ) The activities of Caspase3/7 were determined by Caspase-Glo 3/7 assays in K562 cells (1.5×10 4 ) after transfection of shRNA control or NUDT21 shRNA. ( B ) The percentage of annexin V-positive revealed apoptosis. Percentages of cells undergoing apoptosis in different groups are shown. ( C and D ) Bax and Bcl-2 protein were determined by Western blot analysis in K562 cells after transfection of shRNA control or NUDT21 shRNA. Data are expressed as mean ± SD for duplicate experiments. ** P <0.01; *** P <0.001. Abbreviation: PI, propidium iodide.

Article Snippet: Specific shRNA plasmid against NUDT21 targeting 5′-ACCTCCTCAGTATCCATAT-3′ and a non-silencing shRNA control plasmid targeting 5′-TTCTCCGAACGT-GTCACGT-3′ were synthesized by Santa Cruz Biotechnology Inc.

Techniques: Transfection, shRNA, Western Blot

Journal: Cancer Management and Research

Article Title: Knockdown of NUDT21 inhibits proliferation and promotes apoptosis of human K562 leukemia cells through ERK pathway

doi: 10.2147/CMAR.S173496

Figure Lengend Snippet: Deregulation of signaling pathways upon knockdown of NUDT21. Notes: ( A ) Heatmap of 697 differentially expressed genes after knockdown of NUDT21 in K562 cells. ( B ) Enrichment analysis of differentially expressed genes in GO terms. ( C ) Enrichment analysis of differentially expressed genes in KEGG pathways. ( D ) The histogram showing the relative protein expressions of the key signaling components in K562 cells after transfection of shRNA control or NUDT21 shRNA. Data are expressed as mean ± SD for duplicate experiments. Abbreviations: GO, gene ontology; KEGG, Kyoto Encyclopedia of Genes and Genomics.

Article Snippet: Specific shRNA plasmid against NUDT21 targeting 5′-ACCTCCTCAGTATCCATAT-3′ and a non-silencing shRNA control plasmid targeting 5′-TTCTCCGAACGT-GTCACGT-3′ were synthesized by Santa Cruz Biotechnology Inc.

Techniques: Transfection, shRNA

Journal: Cancer Management and Research

Article Title: Knockdown of NUDT21 inhibits proliferation and promotes apoptosis of human K562 leukemia cells through ERK pathway

doi: 10.2147/CMAR.S173496

Figure Lengend Snippet: Potential mechanisms underlying the effects of NUDT21 on K562 cell growth. Notes: ( A ) The protein levels of p-ERK1/2 and PTEN were shown in K562 cells transfected with shRNA control or NUDT21 shRNA using Western blot analysis. ( B ) The mRNA levels of PTEN were compared between K562 cells of shRNA control group and NUDT21 shRNA group using qRT-PCR analysis. ( C ) BrdU incorporation in K562 cells treated with honokiol or control. ( D ) Percentages of cells undergoing apoptosis (annexin V-positive) in different groups were shown. Data are expressed as mean ± SD for duplicate experiments. * P <0.05; ** P <0.01; *** P <0.001. Abbreviations: BrdU, bromodeoxyuridine; PI, propidium iodide; PTEN, phosphatase and tensin homolog deleted on chromosome 10; qRT-PCR, quantitative reverse polymerase chain reaction.

Article Snippet: Specific shRNA plasmid against NUDT21 targeting 5′-ACCTCCTCAGTATCCATAT-3′ and a non-silencing shRNA control plasmid targeting 5′-TTCTCCGAACGT-GTCACGT-3′ were synthesized by Santa Cruz Biotechnology Inc.

Techniques: Transfection, shRNA, Western Blot, Quantitative RT-PCR, BrdU Incorporation Assay, Polymerase Chain Reaction