Journal: Respiratory Research

Article Title: Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0357-0

Figure Lengend Snippet: TGF-β1 induces WNT10A expression in fibroblasts. a The results of an analysis of Hoechst (blue), WNT10A (red) and Phalloidin (green) staining in LL97A cells as indicated by immunofluorescence. The WNT10A expression after the administration of TGF-β1 (5 ng/ml) in LL97A cells was analyzed using a Western blot analysis. b LL97A and IMR-90 cells were treated with TGF-β1 (5 ng/ml), and at the indicated time points, the mRNA level of WNT10A was assessed by real-time qPCR compared to the untreated cells, and the results are expressed as the means ± SEM. * p < 0.05. c The WNT family genes (WNT3A, WNT5A, WNT7B, WNT10A, WNT10B and β-catenin) were analyzed by real-time qPCR compared to the untreated cells, and the results are expressed as the means ± SEM. * p < 0.05. d The luciferase activity of the WNT10A promoter was measured 24 h after TGF-β1 (5 ng/ml) stimulation, and the results are expressed as the means ± SEM. * p < 0.05

Article Snippet: Specific knockdown was achieved using small interfering RNA against WNT10A (sc-76927) or a control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Inc.).

Techniques: Expressing, Staining, Immunofluorescence, Western Blot, Luciferase, Activity Assay

Journal: Respiratory Research

Article Title: Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0357-0

Figure Lengend Snippet: BLM induces WNT10A and β-catenin expressions. a The mRNA level of WNT10A and β-catenin in BLM-treated and control mice was assessed by real-time qPCR ( n = 10 each). * p < 0.05. b Lung tissue specimens of BLM-treated and control mice were stained with anti-WNT10A and β-catenin antibodies. The arrow indicates the WNT10A and β-catenin expressions in fibroblasts. c COS1 cells stably transfected with WNT10A-luciferase reporter plasmid which can replicate in the cells were treated with the indicated concentration of BLM. After 24 h, a luciferase assay was performed and the results are expressed as the means ± SEM. * p < 0.05. d The transfectants described in ( c ) were treated with 50 uM BLM for the indicated times, and then were changed to a new medium. After 48 h, a luciferase assay was performed as a recovery assay, and the results are expressed as the means ± SEM. * p < 0.05

Article Snippet: Specific knockdown was achieved using small interfering RNA against WNT10A (sc-76927) or a control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Inc.).

Techniques: Staining, Stable Transfection, Transfection, Luciferase, Plasmid Preparation, Concentration Assay

Journal: Respiratory Research

Article Title: Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0357-0

Figure Lengend Snippet: The WNT10A expression regulated collagen production. a Cells were treated with TGF-β1 (5 ng/ml), and at the indicated time points, the mRNA level of COL1A1 was assessed by real-time qPCR. * p < 0.05. b Collagen production was measured 24 h after TGF-β1 (5 ng/ml) stimulation. * p < 0.05. c The efficacy of WNT10A silencing was evaluated by immunoblotting for β-catenin and WNT10A proteins in IMR-90 and LL97A cells. * p < 0.05. d LL97A and IMR-90 cells transfected with indicated siRNAs for 72 h were treated with TGF-β1 (5 ng/ml). After 24 h, the mRNA level of COL1A1 was assessed by real-time qPCR. * p < 0.05. All results are expressed as the means ± SEM

Article Snippet: Specific knockdown was achieved using small interfering RNA against WNT10A (sc-76927) or a control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Inc.).

Techniques: Expressing, Western Blot, Transfection

Journal: Respiratory Research

Article Title: Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0357-0

Figure Lengend Snippet: Comparison between the two groups of IPF patients: positive and negative for WNT10A immunostaining

Article Snippet: Specific knockdown was achieved using small interfering RNA against WNT10A (sc-76927) or a control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Inc.).

Techniques:

Journal: Respiratory Research

Article Title: Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0357-0

Figure Lengend Snippet: The clinicopathological significance of WNT10A expression in patients with IPF. a An immunohistochemical analysis of the WNT10A protein expression in the healthy control and patients with IPF. The left panels show normal human lung tissue treated for the localization of WNT10A using immunohistochemistry reactivity in the smooth muscle of vessels (single arrows) and airways (double arrows). The right upper panels show a WNT10A-positive case, which demonstrated a specific expression of WNT10A in one part of the spindled cells (fibroblasts, indicated by arrows in a high-power view of the right panel) at a site of pulmonary fibroblastic foci (boxes, low-power view of left panel). The right bottom panels indicate a WNT10A-negative case in patients with IPF. b The results of the Kaplan-Meier survival analysis of the overall survival in IPF patients who were WNT10A-positive or negative

Article Snippet: Specific knockdown was achieved using small interfering RNA against WNT10A (sc-76927) or a control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Inc.).

Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry

Journal: Respiratory Research

Article Title: Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0357-0

Figure Lengend Snippet: The results of the correlation analysis between WNT10A and the clinical variables in patients with IPF. The Spearman correlation coefficients between these variables were determined as shown on the plots

Article Snippet: Specific knockdown was achieved using small interfering RNA against WNT10A (sc-76927) or a control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Inc.).

Techniques:

Journal: Respiratory Research

Article Title: Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0357-0

Figure Lengend Snippet: The correlation between WNT10A and the clinical variables in patients with IPF in the WNT10A-positive group. No significant correlations were noted between these clinical variables and positivity of WNT10A based on the Spearman’s correlation coefficients

Article Snippet: Specific knockdown was achieved using small interfering RNA against WNT10A (sc-76927) or a control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Inc.).

Techniques:

Journal: Respiratory Research

Article Title: Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0357-0

Figure Lengend Snippet: Logistic regression analysis for the factors predicting AE-IPF

Article Snippet: Specific knockdown was achieved using small interfering RNA against WNT10A (sc-76927) or a control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Inc.).

Techniques:

Journal: Respiratory Research

Article Title: Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-016-0357-0

Figure Lengend Snippet: The role of WNT10A in lung fibrosis. A proposal model depicting the role of WNT10A in lung fibrosis is shown. Injury leads to increased WNT10A expression, either directly or via TGF-β1 signaling, in fibroblasts. Aberrant activation of WNT10A is involved in mediating the fibrogenesis in patients with IPF by inducing the accumulation of collagen and fibronectin

Article Snippet: Specific knockdown was achieved using small interfering RNA against WNT10A (sc-76927) or a control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Inc.).

Techniques: Expressing, Activation Assay