Review





Similar Products

mouse muc1 elisa kit  (Novus Biologicals)
93
Novus Biologicals mouse muc1 elisa kit
Analysis of mucin production in vaginal tract of OVX mice treated with NET-EN, DMPA or left untreated. OVX mice were treated with NET-EN or DMPA or left untreated (UT) and vaginal washes collected after 1 and 3 weeks of treatment. Some of the treated mice were euthanized and vaginal tissues collected and processed for histological staining with PAS and immunohistochemical staining for <t>Muc1</t> to detect cell associated Mucin. (A) Vaginal washes taken at 1 and 3 weeks were assessed for secreted Muc1 protein levels by commercial mouse Muc1 <t>ELISA</t> kit. Bars indicate mean ± SEM for n = 6 samples. Statistical significance: *, P <0.05. (B) Vaginal tissues showing no Muc1 immunohistochemistry staining in vaginal sections taken from estrus stage serves as negative control and Muc1 staining was visible (dark brown) in diestrus used as a positive control. (C) Vaginal tissues showing different levels of cell associated mucins (multiple mucin types) as detected by intensity of PAS staining (dark pink) and Muc1 immunohistochemistry staining (dark brown) after 1 and 3 weeks of treatment. (D) PAS and Muc1 staining intensities for images of vaginal tissues for different treatments showed in panel (C) were quantified using Fiji ImageJ software as depicted in bar graphs. Bars indicate mean ± SEM for n=9 samples (3 images per mouse vaginal tissue, n=3 mice per treatment). All data are drawn from 2 independent experiments. Data were analyzed utilizing the one-way ANOVA with Tukey’s multiple comparison test. *, P <0.05; **, P <0.01; ***, P <0.001; ****, P<0.0001.
Mouse Muc1 Elisa Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse muc1 elisa kit/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
mouse muc1 elisa kit - by Bioz Stars, 2025-07
93/100 stars
  Buy from Supplier
il 23  (Novus Biologicals)
93
Novus Biologicals il 23
Analysis of mucin production in vaginal tract of OVX mice treated with NET-EN, DMPA or left untreated. OVX mice were treated with NET-EN or DMPA or left untreated (UT) and vaginal washes collected after 1 and 3 weeks of treatment. Some of the treated mice were euthanized and vaginal tissues collected and processed for histological staining with PAS and immunohistochemical staining for <t>Muc1</t> to detect cell associated Mucin. (A) Vaginal washes taken at 1 and 3 weeks were assessed for secreted Muc1 protein levels by commercial mouse Muc1 <t>ELISA</t> kit. Bars indicate mean ± SEM for n = 6 samples. Statistical significance: *, P <0.05. (B) Vaginal tissues showing no Muc1 immunohistochemistry staining in vaginal sections taken from estrus stage serves as negative control and Muc1 staining was visible (dark brown) in diestrus used as a positive control. (C) Vaginal tissues showing different levels of cell associated mucins (multiple mucin types) as detected by intensity of PAS staining (dark pink) and Muc1 immunohistochemistry staining (dark brown) after 1 and 3 weeks of treatment. (D) PAS and Muc1 staining intensities for images of vaginal tissues for different treatments showed in panel (C) were quantified using Fiji ImageJ software as depicted in bar graphs. Bars indicate mean ± SEM for n=9 samples (3 images per mouse vaginal tissue, n=3 mice per treatment). All data are drawn from 2 independent experiments. Data were analyzed utilizing the one-way ANOVA with Tukey’s multiple comparison test. *, P <0.05; **, P <0.01; ***, P <0.001; ****, P<0.0001.
Il 23, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il 23/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
il 23 - by Bioz Stars, 2025-07
93/100 stars
  Buy from Supplier
ki 67  (Novus Biologicals)
93
Novus Biologicals ki 67
Analysis of mucin production in vaginal tract of OVX mice treated with NET-EN, DMPA or left untreated. OVX mice were treated with NET-EN or DMPA or left untreated (UT) and vaginal washes collected after 1 and 3 weeks of treatment. Some of the treated mice were euthanized and vaginal tissues collected and processed for histological staining with PAS and immunohistochemical staining for <t>Muc1</t> to detect cell associated Mucin. (A) Vaginal washes taken at 1 and 3 weeks were assessed for secreted Muc1 protein levels by commercial mouse Muc1 <t>ELISA</t> kit. Bars indicate mean ± SEM for n = 6 samples. Statistical significance: *, P <0.05. (B) Vaginal tissues showing no Muc1 immunohistochemistry staining in vaginal sections taken from estrus stage serves as negative control and Muc1 staining was visible (dark brown) in diestrus used as a positive control. (C) Vaginal tissues showing different levels of cell associated mucins (multiple mucin types) as detected by intensity of PAS staining (dark pink) and Muc1 immunohistochemistry staining (dark brown) after 1 and 3 weeks of treatment. (D) PAS and Muc1 staining intensities for images of vaginal tissues for different treatments showed in panel (C) were quantified using Fiji ImageJ software as depicted in bar graphs. Bars indicate mean ± SEM for n=9 samples (3 images per mouse vaginal tissue, n=3 mice per treatment). All data are drawn from 2 independent experiments. Data were analyzed utilizing the one-way ANOVA with Tukey’s multiple comparison test. *, P <0.05; **, P <0.01; ***, P <0.001; ****, P<0.0001.
Ki 67, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ki 67/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
ki 67 - by Bioz Stars, 2025-07
93/100 stars
  Buy from Supplier

Image Search Results


Analysis of mucin production in vaginal tract of OVX mice treated with NET-EN, DMPA or left untreated. OVX mice were treated with NET-EN or DMPA or left untreated (UT) and vaginal washes collected after 1 and 3 weeks of treatment. Some of the treated mice were euthanized and vaginal tissues collected and processed for histological staining with PAS and immunohistochemical staining for Muc1 to detect cell associated Mucin. (A) Vaginal washes taken at 1 and 3 weeks were assessed for secreted Muc1 protein levels by commercial mouse Muc1 ELISA kit. Bars indicate mean ± SEM for n = 6 samples. Statistical significance: *, P <0.05. (B) Vaginal tissues showing no Muc1 immunohistochemistry staining in vaginal sections taken from estrus stage serves as negative control and Muc1 staining was visible (dark brown) in diestrus used as a positive control. (C) Vaginal tissues showing different levels of cell associated mucins (multiple mucin types) as detected by intensity of PAS staining (dark pink) and Muc1 immunohistochemistry staining (dark brown) after 1 and 3 weeks of treatment. (D) PAS and Muc1 staining intensities for images of vaginal tissues for different treatments showed in panel (C) were quantified using Fiji ImageJ software as depicted in bar graphs. Bars indicate mean ± SEM for n=9 samples (3 images per mouse vaginal tissue, n=3 mice per treatment). All data are drawn from 2 independent experiments. Data were analyzed utilizing the one-way ANOVA with Tukey’s multiple comparison test. *, P <0.05; **, P <0.01; ***, P <0.001; ****, P<0.0001.

Journal: Frontiers in Immunology

Article Title: NET-EN treatment leads to delayed HSV-2 infection, enhanced mucin and T cell functions in the female genital tract when compared to DMPA in a preclinical mouse model

doi: 10.3389/fimmu.2024.1427842

Figure Lengend Snippet: Analysis of mucin production in vaginal tract of OVX mice treated with NET-EN, DMPA or left untreated. OVX mice were treated with NET-EN or DMPA or left untreated (UT) and vaginal washes collected after 1 and 3 weeks of treatment. Some of the treated mice were euthanized and vaginal tissues collected and processed for histological staining with PAS and immunohistochemical staining for Muc1 to detect cell associated Mucin. (A) Vaginal washes taken at 1 and 3 weeks were assessed for secreted Muc1 protein levels by commercial mouse Muc1 ELISA kit. Bars indicate mean ± SEM for n = 6 samples. Statistical significance: *, P <0.05. (B) Vaginal tissues showing no Muc1 immunohistochemistry staining in vaginal sections taken from estrus stage serves as negative control and Muc1 staining was visible (dark brown) in diestrus used as a positive control. (C) Vaginal tissues showing different levels of cell associated mucins (multiple mucin types) as detected by intensity of PAS staining (dark pink) and Muc1 immunohistochemistry staining (dark brown) after 1 and 3 weeks of treatment. (D) PAS and Muc1 staining intensities for images of vaginal tissues for different treatments showed in panel (C) were quantified using Fiji ImageJ software as depicted in bar graphs. Bars indicate mean ± SEM for n=9 samples (3 images per mouse vaginal tissue, n=3 mice per treatment). All data are drawn from 2 independent experiments. Data were analyzed utilizing the one-way ANOVA with Tukey’s multiple comparison test. *, P <0.05; **, P <0.01; ***, P <0.001; ****, P<0.0001.

Article Snippet: Muc1 concentrations in the vaginal washes was measured by mouse Muc1 ELISA kit (Novus Biologicals, Cedarlane, Burlington, ON) according to the manufacturer’s instruction.

Techniques: Staining, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Negative Control, Positive Control, Software, Comparison

Immuno-histochemical analyses of mucin production and HSV-2 virus detection in the vaginal tract of OVX mice treated with NET-EN, DMPA or left untreated and subsequently infected with WT HSV-2. WT C57BL/6 OVX mice (n=5/group) were treated with NET-EN, DMPA or were left untreated (UT) for 2 weeks and then challenged intravaginally with WT HSV-2 333 (5 x 10 3 PFU/mouse). Mice were monitored daily for pathology using the 0 to 5 scale (see Methods). Mice for all 3 groups were euthanized at day 5 post HSV-2 challenge when majority of the mice from DMPA and untreated groups reached stage 4/5 HSV-2 pathological score. Vaginal tissues were collected and processed for immuno-histological staining with Muc1 and HSV-2 antibodies. The experiments were repeated twice with similar results and representative slides for different treatment groups are displayed. (A) Vaginal tissues showing different levels of cell associated mucins (multiple mucin types) as detected by intensity of PAS staining (top images, dark pink stain) and Muc1 immunohistochemistry staining (middle images, dark brown stain), and HSV-2 immunohistochemistry staining (bottom images, dark brown stain). (B) PAS, Muc1 and HSV-2 immunohistochemistry staining intensities for images of vaginal tissues for different treatments exhibited in panel A were quantified by Fiji ImageJ software as shown in bar graphs. Bars indicate mean ± SEM for n=10 images (n=5 mice per treatment and 2 images per vaginal tissue). All data are drawn from 2 independent experiments. Data were analyzed utilizing the one-way ANOVA with Tukey’s multiple comparison test. *, P <0.05; **, P <0.01; ****, P <0.0001.

Journal: Frontiers in Immunology

Article Title: NET-EN treatment leads to delayed HSV-2 infection, enhanced mucin and T cell functions in the female genital tract when compared to DMPA in a preclinical mouse model

doi: 10.3389/fimmu.2024.1427842

Figure Lengend Snippet: Immuno-histochemical analyses of mucin production and HSV-2 virus detection in the vaginal tract of OVX mice treated with NET-EN, DMPA or left untreated and subsequently infected with WT HSV-2. WT C57BL/6 OVX mice (n=5/group) were treated with NET-EN, DMPA or were left untreated (UT) for 2 weeks and then challenged intravaginally with WT HSV-2 333 (5 x 10 3 PFU/mouse). Mice were monitored daily for pathology using the 0 to 5 scale (see Methods). Mice for all 3 groups were euthanized at day 5 post HSV-2 challenge when majority of the mice from DMPA and untreated groups reached stage 4/5 HSV-2 pathological score. Vaginal tissues were collected and processed for immuno-histological staining with Muc1 and HSV-2 antibodies. The experiments were repeated twice with similar results and representative slides for different treatment groups are displayed. (A) Vaginal tissues showing different levels of cell associated mucins (multiple mucin types) as detected by intensity of PAS staining (top images, dark pink stain) and Muc1 immunohistochemistry staining (middle images, dark brown stain), and HSV-2 immunohistochemistry staining (bottom images, dark brown stain). (B) PAS, Muc1 and HSV-2 immunohistochemistry staining intensities for images of vaginal tissues for different treatments exhibited in panel A were quantified by Fiji ImageJ software as shown in bar graphs. Bars indicate mean ± SEM for n=10 images (n=5 mice per treatment and 2 images per vaginal tissue). All data are drawn from 2 independent experiments. Data were analyzed utilizing the one-way ANOVA with Tukey’s multiple comparison test. *, P <0.05; **, P <0.01; ****, P <0.0001.

Article Snippet: Muc1 concentrations in the vaginal washes was measured by mouse Muc1 ELISA kit (Novus Biologicals, Cedarlane, Burlington, ON) according to the manufacturer’s instruction.

Techniques: Virus, Infection, Staining, Immunohistochemistry, Software, Comparison