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pofut1 shrna  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology pofut1 shrna
Figure 1. Evaluation of <t>POFUT1</t> expression in OSCC-derived cell lines. (A) POFUT1 mRNA levels are analyzed in OSCC-derived cells and HNOKs by qRT-PCR. Significant upregulation of POFUT1 mRNA is seen in five OSCC-derived cell lines compared with HNOKs (*P<0.05, Mann- Whitney U test). Data are expressed as the means ± SEM in triplicate. (B) Representative western blot data of POFUT1 in OSCC-derived cell lines and HNOKs. Densitometric POFUT1 protein data are normalized to GAPDH protein levels. The values are expressed as a percentage of the HNOKs. POFUT1 protein is upregulated in OSCC-derived cell lines com pared with HNOKs.
Pofut1 Shrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Evaluation of POFUT1 expression in OSCC-derived cell lines. (A) POFUT1 mRNA levels are analyzed in OSCC-derived cells and HNOKs by qRT-PCR. Significant upregulation of POFUT1 mRNA is seen in five OSCC-derived cell lines compared with HNOKs (*P<0.05, Mann- Whitney U test). Data are expressed as the means ± SEM in triplicate. (B) Representative western blot data of POFUT1 in OSCC-derived cell lines and HNOKs. Densitometric POFUT1 protein data are normalized to GAPDH protein levels. The values are expressed as a percentage of the HNOKs. POFUT1 protein is upregulated in OSCC-derived cell lines com pared with HNOKs.

Journal: International journal of oncology

Article Title: Protein O-fucosyltransferase 1: a potential diagnostic marker and therapeutic target for human oral cancer.

doi: 10.3892/ijo.2013.2110

Figure Lengend Snippet: Figure 1. Evaluation of POFUT1 expression in OSCC-derived cell lines. (A) POFUT1 mRNA levels are analyzed in OSCC-derived cells and HNOKs by qRT-PCR. Significant upregulation of POFUT1 mRNA is seen in five OSCC-derived cell lines compared with HNOKs (*P<0.05, Mann- Whitney U test). Data are expressed as the means ± SEM in triplicate. (B) Representative western blot data of POFUT1 in OSCC-derived cell lines and HNOKs. Densitometric POFUT1 protein data are normalized to GAPDH protein levels. The values are expressed as a percentage of the HNOKs. POFUT1 protein is upregulated in OSCC-derived cell lines com pared with HNOKs.

Article Snippet: The OSCC cell lines, KOSC2 and HSC-2, in which POFUT1 protein expression was higher than in the other cell lines, were stably transfected with POFUT1 shRNA (shPOFUT1) or control shRNA (mock) (Santa Cruz Biotechnology) using Lipofectamine LTX and Plus Reagents (Invitrogen).

Techniques: Expressing, Derivative Assay, Quantitative RT-PCR, MANN-WHITNEY, Western Blot

Figure 2. Evaluation of POFUT1 protein expression in primary OSCCs. (A) Representative IHC results of POFUT1 in normal oral tissue and primary OSCC. (a) Normal oral tissues exhibit negative POFUT1 protein expression. Skeletal muscle tissues are immunostained for POFUT1 (positive control). Original magnification, x100. Scale bars, 50 µm. (b) Positive immunoreactivity for POFUT1 in OSCCs is detected in the cytoplasm. Original magnification, x100. Scale bars, 50 µm. (B) State of POFUT1 protein expression in normal oral tissues (n=128) and primary OSCCs (n=128). The POFUT1 IHC scores are calculated as follows: IHC score = 1 x (number of weakly stained cells in the field) + 2 x (number of moderately stained cells in the field) + 3 x (number of intensely stained cells in the field). The POFUT1 IHC scores for normal oral tissues and OSCCs range from 76.2 to 180.0 (median, 165.8) and 112.5 to 225.5 (median, 192.4), respectively. The POFUT1 protein expression level in OSCCs is significantly (P<0.05, Mann-Whitney U test) higher than that in normal oral tissues. (C) The POFUT1 IHC scores for T3/T4 (149.8 to 240; median, 204.5) are significantly (P<0.05, Mann-Whitney U test) higher than those of T1/T2 (77.8 to 215; median, 189.5).

Journal: International journal of oncology

Article Title: Protein O-fucosyltransferase 1: a potential diagnostic marker and therapeutic target for human oral cancer.

doi: 10.3892/ijo.2013.2110

Figure Lengend Snippet: Figure 2. Evaluation of POFUT1 protein expression in primary OSCCs. (A) Representative IHC results of POFUT1 in normal oral tissue and primary OSCC. (a) Normal oral tissues exhibit negative POFUT1 protein expression. Skeletal muscle tissues are immunostained for POFUT1 (positive control). Original magnification, x100. Scale bars, 50 µm. (b) Positive immunoreactivity for POFUT1 in OSCCs is detected in the cytoplasm. Original magnification, x100. Scale bars, 50 µm. (B) State of POFUT1 protein expression in normal oral tissues (n=128) and primary OSCCs (n=128). The POFUT1 IHC scores are calculated as follows: IHC score = 1 x (number of weakly stained cells in the field) + 2 x (number of moderately stained cells in the field) + 3 x (number of intensely stained cells in the field). The POFUT1 IHC scores for normal oral tissues and OSCCs range from 76.2 to 180.0 (median, 165.8) and 112.5 to 225.5 (median, 192.4), respectively. The POFUT1 protein expression level in OSCCs is significantly (P<0.05, Mann-Whitney U test) higher than that in normal oral tissues. (C) The POFUT1 IHC scores for T3/T4 (149.8 to 240; median, 204.5) are significantly (P<0.05, Mann-Whitney U test) higher than those of T1/T2 (77.8 to 215; median, 189.5).

Article Snippet: The OSCC cell lines, KOSC2 and HSC-2, in which POFUT1 protein expression was higher than in the other cell lines, were stably transfected with POFUT1 shRNA (shPOFUT1) or control shRNA (mock) (Santa Cruz Biotechnology) using Lipofectamine LTX and Plus Reagents (Invitrogen).

Techniques: Expressing, Positive Control, Staining, MANN-WHITNEY

Figure 3. Expression of POFUT1 in shPOFUT1-transfected cells. (A) qRT-PCR shows that POFUT1 mRNA expression in the shPOFUT1-transfected cells (KOSC2- and HSC-2-derived tranfectant cells; two clones each) is significantly (*P<0.05, Mann-Whitney U test) lower than that in the mock-transfected cells. (B) Western blot analysis shows that the POFUT1 protein levels in the shPOFUT1-transfected cells (KOSC2- and HSC-2-derived transfectant cells; two clones each) also have decreased markedly compared with that in the mock-transfected cells.

Journal: International journal of oncology

Article Title: Protein O-fucosyltransferase 1: a potential diagnostic marker and therapeutic target for human oral cancer.

doi: 10.3892/ijo.2013.2110

Figure Lengend Snippet: Figure 3. Expression of POFUT1 in shPOFUT1-transfected cells. (A) qRT-PCR shows that POFUT1 mRNA expression in the shPOFUT1-transfected cells (KOSC2- and HSC-2-derived tranfectant cells; two clones each) is significantly (*P<0.05, Mann-Whitney U test) lower than that in the mock-transfected cells. (B) Western blot analysis shows that the POFUT1 protein levels in the shPOFUT1-transfected cells (KOSC2- and HSC-2-derived transfectant cells; two clones each) also have decreased markedly compared with that in the mock-transfected cells.

Article Snippet: The OSCC cell lines, KOSC2 and HSC-2, in which POFUT1 protein expression was higher than in the other cell lines, were stably transfected with POFUT1 shRNA (shPOFUT1) or control shRNA (mock) (Santa Cruz Biotechnology) using Lipofectamine LTX and Plus Reagents (Invitrogen).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Derivative Assay, Clone Assay, MANN-WHITNEY, Western Blot