Journal: bioRxiv

Article Title: Loss of JAK1 Function Causes G2/M Cell Cycle Defects Vulnerable to Kif18a Inhibition

doi: 10.1101/2025.02.19.638911

Figure Lengend Snippet: ( A ) Schematic of radiation CRISPR-Cas9 screening methodology. Cal27 and Detroit562 HSNCC cell lines were engineered to stably express Cas9, transduced with a kinome-wide gRNA library, and irradiated with 2 Gy for four consecutive treatments. Two weeks after treatment, gRNAs were isolated, PCR amplified, and sent for deep sequencing in parallel with unirradiated controls. Simplified screening results are shown for ( B ) Cal27 and ( C ) Detroit562 cell lines. Hits with an FDR ≤ 0.01 were considered significant. ( D ) Summary of pathways identified by the CRISPR screens including ( i) DNA repair, ( ii ) NFKB signaling, and ( iii ) JAK signaling.

Article Snippet: The Human Kinome CRISPR Knockout Library (Brunello) was purchased from Addgene (# 1000000082) and was generated as previously described .

Techniques: CRISPR, Stable Transfection, Transduction, Irradiation, Isolation, Amplification, Sequencing