7500 real time polymerase chain reaction system  (Thermo Fisher)


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    Name:
    7500 Real Time PCR System
    Description:
    The 7500 Real Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility This system is a sophisticated platform for users who require extended capabilities and maximum versatility The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores The variable excitation capability allows greater sensitivity for longer wavelength red dyes • Powerful five color platform is calibrated for the broadest range of dyes available FAM ⁄SYBR Green I VIC ⁄JOE NED ⁄ TAMRA ⁄ Cy3 ROX ⁄Texas Red and Cy5 dyes • Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets• Advanced multi componenting algorithm minimizes spectral crosstalk superior for multiplexing• User friendly software includes plate set up wizards multi plate data viewing capabilities and advanced analysis tools to make data processing simple and straightforwardNEW 7500 Software v2 x Now the easy to use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2 x upgrade The 7500 Software v2 x incorporates your favorite StepOne Software features such as a variety of plate setup wizards standard curve dilution and master mix recipe calculators QC flags data filters and email notification when a run is finished The 7500 Software v2 x also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options including multiple peak detection step and hold temperature control and customizable ramp rates The NEW Gene Expression Study package accommodates large studies better than any other instrument software package • Import an unlimited number of Comparative CT relative quantitation files to one study • Group samples and view data both by technical replicate group and biological replicate group • Use any gene s as an endogenous control including averaging multiple controls together • Enter known efficiency values to be factored into the RQ results21 CFR Part 11 Module availableThe SDS v1 4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance while still offering the flexibility of user customizable configuration settings • Individual user log ins can be added for up to four user groups each group with designated permission settings • User customizable permission settings include fourteen system activities e signature authority designation and additional security settings to give you maximum control over your compliance efforts • Audit trails can be enabled or disabled depending on your traceability needs • A selection of e signatures is available to ease e signatures into your workflow Supports Many ApplicationsApplications include gene expression analysis pathogen quantitation SNP genotyping isothermal and ⁄ assays utilizing internal positive controls To facilitate many of these applications Applied Biosystems provides preformulated ready to use quality tested TaqMan assays for use with the 7500 system Now you can reduce your assay optimization efforts Upgrade to High Speed Thermal CyclingAn optional upgrade to the 7500 Fast System is available This 7500 Fast System uses our master mix formulations and enables you to shorten your real time PCR runs to as little as 30 minutes For Research Use Only Not for use in diagnostics procedures
    Catalog Number:
    4351104
    Price:
    None
    Applications:
    Genotyping & Genomic Profiling|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Real-Time PCR Instruments, Software & Calibration|Genotyping Instruments, Software & Calibration|Gene Expression Analysis & Genotyping
    Category:
    Instruments and Equipment
    Buy from Supplier


    Structured Review

    Thermo Fisher 7500 real time polymerase chain reaction system
    The 7500 Real Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility This system is a sophisticated platform for users who require extended capabilities and maximum versatility The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores The variable excitation capability allows greater sensitivity for longer wavelength red dyes • Powerful five color platform is calibrated for the broadest range of dyes available FAM ⁄SYBR Green I VIC ⁄JOE NED ⁄ TAMRA ⁄ Cy3 ROX ⁄Texas Red and Cy5 dyes • Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets• Advanced multi componenting algorithm minimizes spectral crosstalk superior for multiplexing• User friendly software includes plate set up wizards multi plate data viewing capabilities and advanced analysis tools to make data processing simple and straightforwardNEW 7500 Software v2 x Now the easy to use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2 x upgrade The 7500 Software v2 x incorporates your favorite StepOne Software features such as a variety of plate setup wizards standard curve dilution and master mix recipe calculators QC flags data filters and email notification when a run is finished The 7500 Software v2 x also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options including multiple peak detection step and hold temperature control and customizable ramp rates The NEW Gene Expression Study package accommodates large studies better than any other instrument software package • Import an unlimited number of Comparative CT relative quantitation files to one study • Group samples and view data both by technical replicate group and biological replicate group • Use any gene s as an endogenous control including averaging multiple controls together • Enter known efficiency values to be factored into the RQ results21 CFR Part 11 Module availableThe SDS v1 4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance while still offering the flexibility of user customizable configuration settings • Individual user log ins can be added for up to four user groups each group with designated permission settings • User customizable permission settings include fourteen system activities e signature authority designation and additional security settings to give you maximum control over your compliance efforts • Audit trails can be enabled or disabled depending on your traceability needs • A selection of e signatures is available to ease e signatures into your workflow Supports Many ApplicationsApplications include gene expression analysis pathogen quantitation SNP genotyping isothermal and ⁄ assays utilizing internal positive controls To facilitate many of these applications Applied Biosystems provides preformulated ready to use quality tested TaqMan assays for use with the 7500 system Now you can reduce your assay optimization efforts Upgrade to High Speed Thermal CyclingAn optional upgrade to the 7500 Fast System is available This 7500 Fast System uses our master mix formulations and enables you to shorten your real time PCR runs to as little as 30 minutes For Research Use Only Not for use in diagnostics procedures
    https://www.bioz.com/result/7500 real time polymerase chain reaction system/product/Thermo Fisher
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    7500 real time polymerase chain reaction system - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vector
    Article Snippet: .. For quantitative analysis of gene expression, SYBR Green real-time PCR was performed on 7500 Real Time PCR System (Applied Biosystems). ..

    Article Title: Signal transducer and activator of transcription 3 (STAT3) promoter methylation and expression in pituitary adenoma
    Article Snippet: .. Quantitative real-time PCR for STAT3 and β-actin was performed in a Real-Time PCR System “Applied Biosystems 7500 Fast”(Applied Biosystems, USA) with SYBR Green chemistry. .. The qRT-PCR was carried out in a 12 μl of mixture which consisted of 6 μl Maxima SYBR Green/ROX qPCR Master Mix (2X) (ThermoFisher Scientific, USA), 15 ng of the cDNA, nuclease-free water and STAT3 gene-specific primers that were designed according to the published data [ ]: 5′-CATATGCGGCCAGCAAAGAA-3′ (forward), 5′-ATACCTGCTCTGAAGAAACT-3′ (reverse), resulting in a 152 bp PCR amplicon to a total concentration of 0.3 μM.

    Amplification:

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)). .. Target amplification is interpreted from the exponential increase in fluorescence per amplification cycle in contract to background signal.

    Fluorescence:

    Article Title: Polymorphisms and features of cytomegalovirus UL144 and UL146 in congenitally infected neonates with hepatic involvement
    Article Snippet: .. Presence of cytomegalovirus DNA was tested by fluorescence quantitative PCR (Applied Biosystems 7500 Real−Time PCR System, Foster City, CA, USA). ..

    Laser Capture Microdissection:

    Article Title: Brain-Derived Neurotrophic Factor and Supraoptic Vasopressin Neurons in Hyponatremia
    Article Snippet: .. The SONs collected from LCM were used to measure changes in BDNF mRNA and AVP hnRNA using quantitative real-time PCR. .. The RNA was extracted and purified from each sample using ArrayPure Nano-Scale RNA Purification Kit reagents (Epicentre Biotechnologies, Madison, WI, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vector
    Article Snippet: .. For quantitative analysis of gene expression, SYBR Green real-time PCR was performed on 7500 Real Time PCR System (Applied Biosystems). ..

    Article Title: Polymorphisms and features of cytomegalovirus UL144 and UL146 in congenitally infected neonates with hepatic involvement
    Article Snippet: .. Presence of cytomegalovirus DNA was tested by fluorescence quantitative PCR (Applied Biosystems 7500 Real−Time PCR System, Foster City, CA, USA). ..

    Article Title: Signal transducer and activator of transcription 3 (STAT3) promoter methylation and expression in pituitary adenoma
    Article Snippet: .. Quantitative real-time PCR for STAT3 and β-actin was performed in a Real-Time PCR System “Applied Biosystems 7500 Fast”(Applied Biosystems, USA) with SYBR Green chemistry. .. The qRT-PCR was carried out in a 12 μl of mixture which consisted of 6 μl Maxima SYBR Green/ROX qPCR Master Mix (2X) (ThermoFisher Scientific, USA), 15 ng of the cDNA, nuclease-free water and STAT3 gene-specific primers that were designed according to the published data [ ]: 5′-CATATGCGGCCAGCAAAGAA-3′ (forward), 5′-ATACCTGCTCTGAAGAAACT-3′ (reverse), resulting in a 152 bp PCR amplicon to a total concentration of 0.3 μM.

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2
    Article Snippet: .. To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol. ..

    Article Title: Insight into the role of PIKK family members and NF-кB in DNAdamage-induced senescence and senescence-associated secretory phenotype of colon cancer cells
    Article Snippet: .. The reactions were performed with the use of either StepOnePlus ™ Real-Time PCR System (Thermo Fisher Scientific)—in the case of triple knockdown experiments (siRNA against ATM , ATR , and PRKDC )—or with 7500 Real-Time PCR System (Applied Biosystems) in the case of all other RT-PCR experiments. ..

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses
    Article Snippet: .. The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)). .. Target amplification is interpreted from the exponential increase in fluorescence per amplification cycle in contract to background signal.

    Article Title: Brain-Derived Neurotrophic Factor and Supraoptic Vasopressin Neurons in Hyponatremia
    Article Snippet: .. The SONs collected from LCM were used to measure changes in BDNF mRNA and AVP hnRNA using quantitative real-time PCR. .. The RNA was extracted and purified from each sample using ArrayPure Nano-Scale RNA Purification Kit reagents (Epicentre Biotechnologies, Madison, WI, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Insight into the role of PIKK family members and NF-кB in DNAdamage-induced senescence and senescence-associated secretory phenotype of colon cancer cells
    Article Snippet: .. The reactions were performed with the use of either StepOnePlus ™ Real-Time PCR System (Thermo Fisher Scientific)—in the case of triple knockdown experiments (siRNA against ATM , ATR , and PRKDC )—or with 7500 Real-Time PCR System (Applied Biosystems) in the case of all other RT-PCR experiments. ..

    Expressing:

    Article Title: High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vector
    Article Snippet: .. For quantitative analysis of gene expression, SYBR Green real-time PCR was performed on 7500 Real Time PCR System (Applied Biosystems). ..

    Polymerase Chain Reaction:

    Article Title: Use of a bovine genome array to identify new biological pathways for beef marbling in Hanwoo (Korean Cattle)
    Article Snippet: .. The PCR was conducted in ABI 7500 realtime PCR system (Applied Biosystems, USA). ..

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  • 99
    Thermo Fisher quantitative real time pcr
    a Representative digital image of mCherry fluorescence in the SON illustrating a successful injection. b Quantitative real-time <t>PCR</t> data showing <t>BDNF</t> mRNA expression from the SON of rats with successful SON injections. c AVP hnRNA expression from the SONs of rats with successful SON injections. Groups: Sham-ligated rats injected with SCR virus (Sham SCR, n = 5); Sham rats injected with ­shBDNF virus (Sham shBDNF, n = 6); BDL rats injected with SCR virus (BDL SCR, n = 6), and BDL rats injected with shBDNF virus (BDL shBDNF, n = 6). Data are mean ± SEM. * p
    Quantitative Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time pcr/product/Thermo Fisher
    Average 99 stars, based on 3264 article reviews
    Price from $9.99 to $1999.99
    quantitative real time pcr - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher abi 7500 fast dx
    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the <t>ABI</t> 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p
    Abi 7500 Fast Dx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi 7500 fast dx/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    abi 7500 fast dx - by Bioz Stars, 2021-01
    99/100 stars
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    Image Search Results


    a Representative digital image of mCherry fluorescence in the SON illustrating a successful injection. b Quantitative real-time PCR data showing BDNF mRNA expression from the SON of rats with successful SON injections. c AVP hnRNA expression from the SONs of rats with successful SON injections. Groups: Sham-ligated rats injected with SCR virus (Sham SCR, n = 5); Sham rats injected with ­shBDNF virus (Sham shBDNF, n = 6); BDL rats injected with SCR virus (BDL SCR, n = 6), and BDL rats injected with shBDNF virus (BDL shBDNF, n = 6). Data are mean ± SEM. * p

    Journal: Neuroendocrinology

    Article Title: Brain-Derived Neurotrophic Factor and Supraoptic Vasopressin Neurons in Hyponatremia

    doi: 10.1159/000503723

    Figure Lengend Snippet: a Representative digital image of mCherry fluorescence in the SON illustrating a successful injection. b Quantitative real-time PCR data showing BDNF mRNA expression from the SON of rats with successful SON injections. c AVP hnRNA expression from the SONs of rats with successful SON injections. Groups: Sham-ligated rats injected with SCR virus (Sham SCR, n = 5); Sham rats injected with ­shBDNF virus (Sham shBDNF, n = 6); BDL rats injected with SCR virus (BDL SCR, n = 6), and BDL rats injected with shBDNF virus (BDL shBDNF, n = 6). Data are mean ± SEM. * p

    Article Snippet: The SONs collected from LCM were used to measure changes in BDNF mRNA and AVP hnRNA using quantitative real-time PCR.

    Techniques: Fluorescence, Injection, Real-time Polymerase Chain Reaction, Expressing

    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation

    MSK1 inhibition reduces cDC2 activation and inhibits the production of pro-inflammatory cytokines. Isolated cDC2s from buffy coats were treated with H89 (10 μM), SB 747651A (SB; 10 μM), Ro 31-8220 (Ro; 5 μM), or left untreated for 1 h. Then, except for medium control, cells were stimulated with TLR4L (100 ng/mL) for 6 h. FACS was used to assess cell-viability (A) and the expression of CD80 and CD83 shown as median fluorescence intensity (MFI) (B) . Cytokine production upon TLR4L stimulation in the presence or absence of MSK1 inhibitors was measured by qRT-PCR (C) and ELISA (D) . Results are represented as median ± IQR. * p

    Journal: Frontiers in Immunology

    Article Title: MicroRNA-130a Contributes to Type-2 Classical DC-activation in Sjögren's Syndrome by Targeting Mitogen- and Stress-Activated Protein Kinase-1

    doi: 10.3389/fimmu.2019.01335

    Figure Lengend Snippet: MSK1 inhibition reduces cDC2 activation and inhibits the production of pro-inflammatory cytokines. Isolated cDC2s from buffy coats were treated with H89 (10 μM), SB 747651A (SB; 10 μM), Ro 31-8220 (Ro; 5 μM), or left untreated for 1 h. Then, except for medium control, cells were stimulated with TLR4L (100 ng/mL) for 6 h. FACS was used to assess cell-viability (A) and the expression of CD80 and CD83 shown as median fluorescence intensity (MFI) (B) . Cytokine production upon TLR4L stimulation in the presence or absence of MSK1 inhibitors was measured by qRT-PCR (C) and ELISA (D) . Results are represented as median ± IQR. * p

    Article Snippet: Detailed descriptions of stable isotope labeling of amino acids in cell culture (SILAC), selection of in silico predicted miRNA targets, quantitative real-time PCR and cytokine analysis are provided in the Online Supplementary Methods.

    Techniques: Inhibition, Activation Assay, Isolation, FACS, Expressing, Fluorescence, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Article Snippet: Performance of the Trioplex assay was evaluated using RNA extractions from the MagNA Pure LC 2.0, MagNA Pure 96, MagNA Pure Compact, and NucliSENS® easyMag® platforms and run on the ABI 7500 Fast Dx and QuantStudio Dx real-time PCR instruments.

    Techniques: Lysis, Multiplex Assay