7500 real time pcr system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 7500 real time pcr system
    7500 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7500 real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 7060 article reviews
    Price from $9.99 to $1999.99
    7500 real time pcr system - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Intra- and Extracellular Degradation of Neutrophil Extracellular Traps by Macrophages and Dendritic Cells
    Article Snippet: .. Analysis of transcripts of the three different nucleases, as well as downregulation of TREX1 , DNASEII , and DNASE1L3 , was performed with an Applied Biosystems 7500 Real-Time PCR System using the Power SYBR Green PCR Master Mix (Applied Biosystems); analysis of the data were carried out with the Applied Biosystems 7500 Real-Time PCR software v.2.3. .. Primers used were from Sigma-Aldrich and were as follows: GAPDH forward 5′-CCCCTTCATTGACCTCAACTAC-3′; GAPDH reverse 5′-GAGTCCTTCCACGATACCAAAG-3′; DNASEII forward 5′-TTCCTGCTCTACAATGACCAAC-3′; DNASEII reverse 5′-GGAAGTTAGGTACACTGTGGACC-3′; TREX1 forward 5′-GCATCTGTCAGTGGAGACCA-3′; TREX1 reverse 5′-AGATCCTTGGTACCCCTGCT-3′; DNASE1L3 forward 5′-GTGCATATGACAGGATTGTG-3′; and DNASE1L3 reverse 5′-AATTCAACTGGAAAGTGGTC-3′.

    Article Title: Hsp90B enhances MAST1-mediated cisplatin resistance by protecting MAST1 from proteosomal degradation
    Article Snippet: .. Quantitative PCR was performed on a 7500 Real Time PCR System (Applied Biosystems) using iTaq Universal SYBR Green Supermix (Bio-Rad). .. Primers used for MAST1 qPCR were forward 5′-TCTCTGGACCGCGC TTTCTA-3′ and reverse 5′-TGAGGCTTTTCCGATTACTGGT-3′.

    Article Title: Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice
    Article Snippet: .. RT-PCR was performed by a 7500 real-time PCR system (Applied Biosystems, Life Technologies, USA) following a PCR cycle of denaturation at 94°C for 30 seconds, annealing at 60°C for all genes for 60 seconds, and extension at 72°C for 2 minutes for 40 cycles. .. RT-PCR was performed by a 7500 real-time PCR system (Applied Biosystems, Life Technologies, USA) following a PCR cycle of denaturation at 94°C for 30 seconds, annealing at 60°C for all genes for 60 seconds, and extension at 72°C for 2 minutes for 40 cycles.

    Article Title: Pituitary Action of E2 in Prepubertal Grass Carp: Receptor Specificity and Signal Transduction for Luteinizing Hormone and Follicle-Stimulating Hormone Regulation
    Article Snippet: .. The RT samples were subjected to qPCR using a 7500 real time PCR system (Applied Biosystems, USA) with primers specific for grass carp LHβ, FSHβ, and GREB1, respectively (see Table S1 in Supplementary Material for primer sequences and PCR condition). .. In these experiments, serial dilutions of plasmid DNA with the coding sequences for grass carp LHβ, FSHβ, and GREB1 were used as the standards for data calibration, and parallel real-time PCR for β-actin was also conducted to serve as the internal control.

    Article Title: Endogenous IL-10 Maintains Immune Tolerance but IL-10 Gene Transfer Exacerbates Autoimmune Cholangitis
    Article Snippet: .. Amplification was performed with SYBR Green MasterMix (Thermo Scientific, USA) using the 7500 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Metformin Ameliorates Lipotoxic β-Cell Dysfunction through a Concentration-Dependent Dual Mechanism of Action
    Article Snippet: .. Real-time polymerase chain reaction (PCR) was performed using SYBR Premix Ex Taq reagents (TaKaRa, Shiga, Japan) and a 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Glycemic Reduction Alters White Blood Cell Counts and Inflammatory Gene Expression in Diabetes
    Article Snippet: .. Quantity values, calculated from cycle threshold (CT ) values, were based on standard curves obtained for each gene in each sample using the Applied Biosystems 7500 Real-Time PCR System. ..

    Article Title: Loss of GAS5 tumour suppressor lncRNA: an independent molecular cancer biomarker for short-term relapse and progression in bladder cancer patients
    Article Snippet: .. The 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA) was used for the qPCR assays. .. The 10 μl reaction consists of Kapa SYBR® Fast Universal 2X qPCR MasterMix (Kapa Biosystems, Inc., Woburn, MA), 100 nM of each specific PCR primer, and 10 ng of cDNA template.

    Article Title: N-Acetylcysteine Ameliorates Gentamicin-Induced Nephrotoxicity by Enhancing Autophagy and Reducing Oxidative Damage in Miniature Pigs
    Article Snippet: .. Amplification was performed in a 7500 real-time PCR System (Applied Biosystems, Carlsbad, Calif). .. Reaction contained 50 ng total cDNA, 0.2 μM primers, and 10 μL 2× SYBR green buffer (TaKaRa, Shiga, Japan, DRR820A) in a final volume of 20 μL.

    Article Title: DCAF13 promotes pluripotency by negatively regulating SUV39H1 stability during early embryonic development
    Article Snippet: .. Real‐time RT–PCR analysis was performed using a Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems 7500 Real‐Time PCR System. .. Relative mRNA levels were calculated by normalizing to the levels of endogenous β‐actin mRNA (internal control) using Microsoft Excel®.

    Article Title: Colonoscopic-Guided Pinch Biopsies in Mice as a Useful Model for Evaluating the Roles of Host and Luminal Factors in Colonic Inflammation
    Article Snippet: .. Quantitative real-time PCR was conducted using 2× SYBR Green PCR master mix on a 7500 real-time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: Downregulation of CDH16 in Papillary Thyroid Cancer and Its Potential Molecular Mechanism Analysed by qRT-PCR, TCGA and in silico Analysis
    Article Snippet: .. We used ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan) for cDNA preparation. qRT-PCR analysis of CDH16 expression was performed in triplicate using the Thunderbird SYBR qPCR Mix (Toyobo, Osaka, Japan) in the Applied Biosystems 7500 Real-time PCR system (Applied biosystems, Foster City, CA). .. We set the expression of GAPDH as an internal control.

    Article Title: Chlamydiaceae in wild, feral and domestic pigeons in Switzerland and insight into population dynamics by Chlamydia psittaci multilocus sequence typing
    Article Snippet: .. Quantitative and conventional PCRs All quantitative PCRs (qPCR; , ) were run on an Applied Biosystems® 7500 Real-Time PCR System (Thermo Fisher Scientific). .. As internal amplification control, eGFP was added to the reaction mix [ ].

    Article Title: Long Non-coding RNA Maternally Expressed 3 Increases the Expression of Neuron-Specific Genes by Targeting miR-128-3p in All-Trans Retinoic Acid-Induced Neurogenic Differentiation From Amniotic Epithelial Cells
    Article Snippet: .. It was then reverse-transcribed and used for qPCR analysis with the RNA PCR kit version 3.0 (Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR system. ..

    Article Title: 9-ING-41, a small molecule inhibitor of GSK-3beta, potentiates the effects of anticancer therapeutics in bladder cancer
    Article Snippet: .. Real-time quantitative reverse transcriptase-PCR (RT-PCR) was done in the 7500 Real Time PCR System (Applied Biosystems, Waltham, MA) using pre-designed TaqMan Universal PCR Mastermix (Applied Biosystems, Waltham, MA) targeting human Bcl-2 and XIAP mRNA, and GAPDH was used as endogenous control . .. The expression of the target mRNA was quantified relative to that of the GAPDH mRNA .

    RNA Extraction:

    Article Title: Metformin Ameliorates Lipotoxic β-Cell Dysfunction through a Concentration-Dependent Dual Mechanism of Action
    Article Snippet: Paragraph title: RNA extraction and real-time quantitative polymerase chain reaction ... Real-time polymerase chain reaction (PCR) was performed using SYBR Premix Ex Taq reagents (TaKaRa, Shiga, Japan) and a 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA).

    Article Title: 9-ING-41, a small molecule inhibitor of GSK-3beta, potentiates the effects of anticancer therapeutics in bladder cancer
    Article Snippet: Paragraph title: RNA extraction and RT-PCR ... Real-time quantitative reverse transcriptase-PCR (RT-PCR) was done in the 7500 Real Time PCR System (Applied Biosystems, Waltham, MA) using pre-designed TaqMan Universal PCR Mastermix (Applied Biosystems, Waltham, MA) targeting human Bcl-2 and XIAP mRNA, and GAPDH was used as endogenous control .

    Amplification:

    Article Title: Endogenous IL-10 Maintains Immune Tolerance but IL-10 Gene Transfer Exacerbates Autoimmune Cholangitis
    Article Snippet: .. Amplification was performed with SYBR Green MasterMix (Thermo Scientific, USA) using the 7500 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Loss of GAS5 tumour suppressor lncRNA: an independent molecular cancer biomarker for short-term relapse and progression in bladder cancer patients
    Article Snippet: Based on published sequences (NCBI Ref Seq: NR_002578.3 for GAS5 and NM_000194.2 for HPRT1 ) and according to in silico analysis, specific primers for GAS5 (F: 5′-CTTGCCTGGACCAGCTTAAT-3′, R: 5′-CAAGCCGACTCTCCATACCT-3′) and HPRT1 (F: 5′-TGGAAAGGGTGTTTATTCCTCAT, R: 5′-ATGTAATCCAGCAGGTCAGCAA-3′) were designed and used for the amplification of a 122 bp GAS5 -specific and a 151 bp HPRT1 -specific amplicon. .. The 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA) was used for the qPCR assays.

    Article Title: N-Acetylcysteine Ameliorates Gentamicin-Induced Nephrotoxicity by Enhancing Autophagy and Reducing Oxidative Damage in Miniature Pigs
    Article Snippet: .. Amplification was performed in a 7500 real-time PCR System (Applied Biosystems, Carlsbad, Calif). .. Reaction contained 50 ng total cDNA, 0.2 μM primers, and 10 μL 2× SYBR green buffer (TaKaRa, Shiga, Japan, DRR820A) in a final volume of 20 μL.

    Article Title: Colonoscopic-Guided Pinch Biopsies in Mice as a Useful Model for Evaluating the Roles of Host and Luminal Factors in Colonic Inflammation
    Article Snippet: Tnf , Il1β , and Il6 were amplified using the following primer sequences: Tnf , 5′-CCAGACCCTCACACTCAGATC-3′ (forward) and 5′-CACTTGGTGGTTTGCTACGAC-3′ (reverse); Il1β , 5′-TGGGCCTCAAAGGAAAGAAT-3′ (forward) and 5′-CAGGCTTGTGCTCTGCTTGT-3′ (reverse); and Il6 , 5′-CCAGAGATACAAAGAAATGATGG-3′ (forward) and 5′-ACTCCAGAAGACCAGAGGAAAT-3′ (reverse). .. Quantitative real-time PCR was conducted using 2× SYBR Green PCR master mix on a 7500 real-time PCR system (Applied Biosystems, Foster City, CA).

    Article Title: Chlamydiaceae in wild, feral and domestic pigeons in Switzerland and insight into population dynamics by Chlamydia psittaci multilocus sequence typing
    Article Snippet: Quantitative and conventional PCRs All quantitative PCRs (qPCR; , ) were run on an Applied Biosystems® 7500 Real-Time PCR System (Thermo Fisher Scientific). .. As internal amplification control, eGFP was added to the reaction mix [ ].

    Agarose Gel Electrophoresis:

    Article Title: Loss of GAS5 tumour suppressor lncRNA: an independent molecular cancer biomarker for short-term relapse and progression in bladder cancer patients
    Article Snippet: The 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA) was used for the qPCR assays. .. The thermal protocol consisted of polymerase activation step at 95 °C for 3 min, followed by 40 cycles of denaturation at 95 °C for 15 s and finally the primer annealing and extension step at 60 °C for 1 min. Melting curve analysis and agarose gel electrophoresis were performed following amplification to discriminate specific amplicons from non-specific products or primer dimers.

    Synthesized:

    Article Title: 9-ING-41, a small molecule inhibitor of GSK-3beta, potentiates the effects of anticancer therapeutics in bladder cancer
    Article Snippet: RNA extraction and RT-PCR Total cellular RNA was extracted using the SV total RNA Isolation System (Promega, Madison, WI) and first-strand DNA was synthesized using a High Capacity cDNA Reverse Trascription Kit (Applied Biosystems, Waltham, MA) according to the manufacturer’s instructions as described previously . .. Real-time quantitative reverse transcriptase-PCR (RT-PCR) was done in the 7500 Real Time PCR System (Applied Biosystems, Waltham, MA) using pre-designed TaqMan Universal PCR Mastermix (Applied Biosystems, Waltham, MA) targeting human Bcl-2 and XIAP mRNA, and GAPDH was used as endogenous control .

    Software:

    Article Title: Intra- and Extracellular Degradation of Neutrophil Extracellular Traps by Macrophages and Dendritic Cells
    Article Snippet: .. Analysis of transcripts of the three different nucleases, as well as downregulation of TREX1 , DNASEII , and DNASE1L3 , was performed with an Applied Biosystems 7500 Real-Time PCR System using the Power SYBR Green PCR Master Mix (Applied Biosystems); analysis of the data were carried out with the Applied Biosystems 7500 Real-Time PCR software v.2.3. .. Primers used were from Sigma-Aldrich and were as follows: GAPDH forward 5′-CCCCTTCATTGACCTCAACTAC-3′; GAPDH reverse 5′-GAGTCCTTCCACGATACCAAAG-3′; DNASEII forward 5′-TTCCTGCTCTACAATGACCAAC-3′; DNASEII reverse 5′-GGAAGTTAGGTACACTGTGGACC-3′; TREX1 forward 5′-GCATCTGTCAGTGGAGACCA-3′; TREX1 reverse 5′-AGATCCTTGGTACCCCTGCT-3′; DNASE1L3 forward 5′-GTGCATATGACAGGATTGTG-3′; and DNASE1L3 reverse 5′-AATTCAACTGGAAAGTGGTC-3′.

    Article Title: N-Acetylcysteine Ameliorates Gentamicin-Induced Nephrotoxicity by Enhancing Autophagy and Reducing Oxidative Damage in Miniature Pigs
    Article Snippet: Amplification was performed in a 7500 real-time PCR System (Applied Biosystems, Carlsbad, Calif). .. Primers were designed using the software package Primer Express 2.0 (Applied Biosystems, Carlsbad, Calif) based on GenBank nucleotide sequences as follows: Chemokine (C-C motif) ligand (CCL)-5 (Accession: NM_001129946.1): Forward 5’-GTGTGTGCCAACCCAGAGAA-3’, Reverse 5’-GGACAAGAGCAAGAAGCAGTAGG-3’.

    Isolation:

    Article Title: Intra- and Extracellular Degradation of Neutrophil Extracellular Traps by Macrophages and Dendritic Cells
    Article Snippet: Total RNA was extracted from freshly isolated monocytes, HMDMs on days 3 or 4 after isolation, and MDDCs using the QIAGEN RNeasy Mini Kit following the manufacturer’s protocol. .. Analysis of transcripts of the three different nucleases, as well as downregulation of TREX1 , DNASEII , and DNASE1L3 , was performed with an Applied Biosystems 7500 Real-Time PCR System using the Power SYBR Green PCR Master Mix (Applied Biosystems); analysis of the data were carried out with the Applied Biosystems 7500 Real-Time PCR software v.2.3.

    Article Title: Hsp90B enhances MAST1-mediated cisplatin resistance by protecting MAST1 from proteosomal degradation
    Article Snippet: Total RNA from KB-3-1cisR and A549cisR cells was isolated using the RNeasy Kit (Qiagen). .. Quantitative PCR was performed on a 7500 Real Time PCR System (Applied Biosystems) using iTaq Universal SYBR Green Supermix (Bio-Rad).

    Article Title: Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice
    Article Snippet: Total RNA was isolated from mouse liver tissue using an RNA isolation kit (RNAiso, Aidlab Biotechnologies Co., Beijing, China). .. RT-PCR was performed by a 7500 real-time PCR system (Applied Biosystems, Life Technologies, USA) following a PCR cycle of denaturation at 94°C for 30 seconds, annealing at 60°C for all genes for 60 seconds, and extension at 72°C for 2 minutes for 40 cycles.

    Article Title: Endogenous IL-10 Maintains Immune Tolerance but IL-10 Gene Transfer Exacerbates Autoimmune Cholangitis
    Article Snippet: Paragraph title: 2.6. Isolation of mRNA and real-time PCR ... Amplification was performed with SYBR Green MasterMix (Thermo Scientific, USA) using the 7500 Real-Time PCR System (Applied Biosystems).

    Article Title: N-Acetylcysteine Ameliorates Gentamicin-Induced Nephrotoxicity by Enhancing Autophagy and Reducing Oxidative Damage in Miniature Pigs
    Article Snippet: Paragraph title: RNA isolation and real-time quantitative PCR ... Amplification was performed in a 7500 real-time PCR System (Applied Biosystems, Carlsbad, Calif).

    Article Title: DCAF13 promotes pluripotency by negatively regulating SUV39H1 stability during early embryonic development
    Article Snippet: Paragraph title: RNA isolation and real‐time RT–PCR ... Real‐time RT–PCR analysis was performed using a Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems 7500 Real‐Time PCR System.

    Article Title: Long Non-coding RNA Maternally Expressed 3 Increases the Expression of Neuron-Specific Genes by Targeting miR-128-3p in All-Trans Retinoic Acid-Induced Neurogenic Differentiation From Amniotic Epithelial Cells
    Article Snippet: Total RNA was isolated from AECs and induced AECs using TRIzol reagent (Gibco, Carlsbad, CA, United States). .. It was then reverse-transcribed and used for qPCR analysis with the RNA PCR kit version 3.0 (Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR system.

    Article Title: 9-ING-41, a small molecule inhibitor of GSK-3beta, potentiates the effects of anticancer therapeutics in bladder cancer
    Article Snippet: RNA extraction and RT-PCR Total cellular RNA was extracted using the SV total RNA Isolation System (Promega, Madison, WI) and first-strand DNA was synthesized using a High Capacity cDNA Reverse Trascription Kit (Applied Biosystems, Waltham, MA) according to the manufacturer’s instructions as described previously . .. Real-time quantitative reverse transcriptase-PCR (RT-PCR) was done in the 7500 Real Time PCR System (Applied Biosystems, Waltham, MA) using pre-designed TaqMan Universal PCR Mastermix (Applied Biosystems, Waltham, MA) targeting human Bcl-2 and XIAP mRNA, and GAPDH was used as endogenous control .

    Quantitative RT-PCR:

    Article Title: Hsp90B enhances MAST1-mediated cisplatin resistance by protecting MAST1 from proteosomal degradation
    Article Snippet: Paragraph title: Quantitative RT-PCR. ... Quantitative PCR was performed on a 7500 Real Time PCR System (Applied Biosystems) using iTaq Universal SYBR Green Supermix (Bio-Rad).

    Article Title: DCAF13 promotes pluripotency by negatively regulating SUV39H1 stability during early embryonic development
    Article Snippet: .. Real‐time RT–PCR analysis was performed using a Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems 7500 Real‐Time PCR System. .. Relative mRNA levels were calculated by normalizing to the levels of endogenous β‐actin mRNA (internal control) using Microsoft Excel®.

    Article Title: Downregulation of CDH16 in Papillary Thyroid Cancer and Its Potential Molecular Mechanism Analysed by qRT-PCR, TCGA and in silico Analysis
    Article Snippet: .. We used ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan) for cDNA preparation. qRT-PCR analysis of CDH16 expression was performed in triplicate using the Thunderbird SYBR qPCR Mix (Toyobo, Osaka, Japan) in the Applied Biosystems 7500 Real-time PCR system (Applied biosystems, Foster City, CA). .. We set the expression of GAPDH as an internal control.

    Article Title: Long Non-coding RNA Maternally Expressed 3 Increases the Expression of Neuron-Specific Genes by Targeting miR-128-3p in All-Trans Retinoic Acid-Induced Neurogenic Differentiation From Amniotic Epithelial Cells
    Article Snippet: The expression of genes upstream and downstream of miR-128-3p [β III-tubulin, glial fibrillary acidic protein (GFAP ), Jagged 1 (JAG1 ), and MEG3 ] was also tested using RT-qPCR. .. It was then reverse-transcribed and used for qPCR analysis with the RNA PCR kit version 3.0 (Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR system.

    Purification:

    Article Title: Chlamydiaceae in wild, feral and domestic pigeons in Switzerland and insight into population dynamics by Chlamydia psittaci multilocus sequence typing
    Article Snippet: Quantitative and conventional PCRs All quantitative PCRs (qPCR; , ) were run on an Applied Biosystems® 7500 Real-Time PCR System (Thermo Fisher Scientific). .. Products from all conventional PCRs ( , ) were purified using the QIAquick® PCR Purification Kit (Qiagen) according to the manufacturer’s instructions.

    SYBR Green Assay:

    Article Title: Intra- and Extracellular Degradation of Neutrophil Extracellular Traps by Macrophages and Dendritic Cells
    Article Snippet: .. Analysis of transcripts of the three different nucleases, as well as downregulation of TREX1 , DNASEII , and DNASE1L3 , was performed with an Applied Biosystems 7500 Real-Time PCR System using the Power SYBR Green PCR Master Mix (Applied Biosystems); analysis of the data were carried out with the Applied Biosystems 7500 Real-Time PCR software v.2.3. .. Primers used were from Sigma-Aldrich and were as follows: GAPDH forward 5′-CCCCTTCATTGACCTCAACTAC-3′; GAPDH reverse 5′-GAGTCCTTCCACGATACCAAAG-3′; DNASEII forward 5′-TTCCTGCTCTACAATGACCAAC-3′; DNASEII reverse 5′-GGAAGTTAGGTACACTGTGGACC-3′; TREX1 forward 5′-GCATCTGTCAGTGGAGACCA-3′; TREX1 reverse 5′-AGATCCTTGGTACCCCTGCT-3′; DNASE1L3 forward 5′-GTGCATATGACAGGATTGTG-3′; and DNASE1L3 reverse 5′-AATTCAACTGGAAAGTGGTC-3′.

    Article Title: Hsp90B enhances MAST1-mediated cisplatin resistance by protecting MAST1 from proteosomal degradation
    Article Snippet: .. Quantitative PCR was performed on a 7500 Real Time PCR System (Applied Biosystems) using iTaq Universal SYBR Green Supermix (Bio-Rad). .. Primers used for MAST1 qPCR were forward 5′-TCTCTGGACCGCGC TTTCTA-3′ and reverse 5′-TGAGGCTTTTCCGATTACTGGT-3′.

    Article Title: Endogenous IL-10 Maintains Immune Tolerance but IL-10 Gene Transfer Exacerbates Autoimmune Cholangitis
    Article Snippet: .. Amplification was performed with SYBR Green MasterMix (Thermo Scientific, USA) using the 7500 Real-Time PCR System (Applied Biosystems). ..

    Article Title: Loss of GAS5 tumour suppressor lncRNA: an independent molecular cancer biomarker for short-term relapse and progression in bladder cancer patients
    Article Snippet: Quantitative real-time PCR A SYBR-Green fluorescent-based quantitative real-time PCR (qPCR) assays was developed and applied to assess GAS5 expression. .. The 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA) was used for the qPCR assays.

    Article Title: N-Acetylcysteine Ameliorates Gentamicin-Induced Nephrotoxicity by Enhancing Autophagy and Reducing Oxidative Damage in Miniature Pigs
    Article Snippet: Amplification was performed in a 7500 real-time PCR System (Applied Biosystems, Carlsbad, Calif). .. Reaction contained 50 ng total cDNA, 0.2 μM primers, and 10 μL 2× SYBR green buffer (TaKaRa, Shiga, Japan, DRR820A) in a final volume of 20 μL.

    Article Title: DCAF13 promotes pluripotency by negatively regulating SUV39H1 stability during early embryonic development
    Article Snippet: .. Real‐time RT–PCR analysis was performed using a Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems 7500 Real‐Time PCR System. .. Relative mRNA levels were calculated by normalizing to the levels of endogenous β‐actin mRNA (internal control) using Microsoft Excel®.

    Article Title: Colonoscopic-Guided Pinch Biopsies in Mice as a Useful Model for Evaluating the Roles of Host and Luminal Factors in Colonic Inflammation
    Article Snippet: .. Quantitative real-time PCR was conducted using 2× SYBR Green PCR master mix on a 7500 real-time PCR system (Applied Biosystems, Foster City, CA). ..

    Activation Assay:

    Article Title: Loss of GAS5 tumour suppressor lncRNA: an independent molecular cancer biomarker for short-term relapse and progression in bladder cancer patients
    Article Snippet: The 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA) was used for the qPCR assays. .. The thermal protocol consisted of polymerase activation step at 95 °C for 3 min, followed by 40 cycles of denaturation at 95 °C for 15 s and finally the primer annealing and extension step at 60 °C for 1 min. Melting curve analysis and agarose gel electrophoresis were performed following amplification to discriminate specific amplicons from non-specific products or primer dimers.

    Generated:

    Article Title: Endogenous IL-10 Maintains Immune Tolerance but IL-10 Gene Transfer Exacerbates Autoimmune Cholangitis
    Article Snippet: The cDNA was generated by oligonucleotide priming using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems, Foster City, CA, USA). .. Amplification was performed with SYBR Green MasterMix (Thermo Scientific, USA) using the 7500 Real-Time PCR System (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: Intra- and Extracellular Degradation of Neutrophil Extracellular Traps by Macrophages and Dendritic Cells
    Article Snippet: .. Analysis of transcripts of the three different nucleases, as well as downregulation of TREX1 , DNASEII , and DNASE1L3 , was performed with an Applied Biosystems 7500 Real-Time PCR System using the Power SYBR Green PCR Master Mix (Applied Biosystems); analysis of the data were carried out with the Applied Biosystems 7500 Real-Time PCR software v.2.3. .. Primers used were from Sigma-Aldrich and were as follows: GAPDH forward 5′-CCCCTTCATTGACCTCAACTAC-3′; GAPDH reverse 5′-GAGTCCTTCCACGATACCAAAG-3′; DNASEII forward 5′-TTCCTGCTCTACAATGACCAAC-3′; DNASEII reverse 5′-GGAAGTTAGGTACACTGTGGACC-3′; TREX1 forward 5′-GCATCTGTCAGTGGAGACCA-3′; TREX1 reverse 5′-AGATCCTTGGTACCCCTGCT-3′; DNASE1L3 forward 5′-GTGCATATGACAGGATTGTG-3′; and DNASE1L3 reverse 5′-AATTCAACTGGAAAGTGGTC-3′.

    Article Title: Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice
    Article Snippet: .. RT-PCR was performed by a 7500 real-time PCR system (Applied Biosystems, Life Technologies, USA) following a PCR cycle of denaturation at 94°C for 30 seconds, annealing at 60°C for all genes for 60 seconds, and extension at 72°C for 2 minutes for 40 cycles. .. RT-PCR was performed by a 7500 real-time PCR system (Applied Biosystems, Life Technologies, USA) following a PCR cycle of denaturation at 94°C for 30 seconds, annealing at 60°C for all genes for 60 seconds, and extension at 72°C for 2 minutes for 40 cycles.

    Article Title: Pituitary Action of E2 in Prepubertal Grass Carp: Receptor Specificity and Signal Transduction for Luteinizing Hormone and Follicle-Stimulating Hormone Regulation
    Article Snippet: .. The RT samples were subjected to qPCR using a 7500 real time PCR system (Applied Biosystems, USA) with primers specific for grass carp LHβ, FSHβ, and GREB1, respectively (see Table S1 in Supplementary Material for primer sequences and PCR condition). .. In these experiments, serial dilutions of plasmid DNA with the coding sequences for grass carp LHβ, FSHβ, and GREB1 were used as the standards for data calibration, and parallel real-time PCR for β-actin was also conducted to serve as the internal control.

    Article Title: Endogenous IL-10 Maintains Immune Tolerance but IL-10 Gene Transfer Exacerbates Autoimmune Cholangitis
    Article Snippet: Amplification was performed with SYBR Green MasterMix (Thermo Scientific, USA) using the 7500 Real-Time PCR System (Applied Biosystems). .. Primer sequences used in PCR are as described previously [ ].

    Article Title: Metformin Ameliorates Lipotoxic β-Cell Dysfunction through a Concentration-Dependent Dual Mechanism of Action
    Article Snippet: .. Real-time polymerase chain reaction (PCR) was performed using SYBR Premix Ex Taq reagents (TaKaRa, Shiga, Japan) and a 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Loss of GAS5 tumour suppressor lncRNA: an independent molecular cancer biomarker for short-term relapse and progression in bladder cancer patients
    Article Snippet: The 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA) was used for the qPCR assays. .. The 10 μl reaction consists of Kapa SYBR® Fast Universal 2X qPCR MasterMix (Kapa Biosystems, Inc., Woburn, MA), 100 nM of each specific PCR primer, and 10 ng of cDNA template.

    Article Title: DCAF13 promotes pluripotency by negatively regulating SUV39H1 stability during early embryonic development
    Article Snippet: .. Real‐time RT–PCR analysis was performed using a Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies) and an Applied Biosystems 7500 Real‐Time PCR System. .. Relative mRNA levels were calculated by normalizing to the levels of endogenous β‐actin mRNA (internal control) using Microsoft Excel®.

    Article Title: Colonoscopic-Guided Pinch Biopsies in Mice as a Useful Model for Evaluating the Roles of Host and Luminal Factors in Colonic Inflammation
    Article Snippet: .. Quantitative real-time PCR was conducted using 2× SYBR Green PCR master mix on a 7500 real-time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: Chlamydiaceae in wild, feral and domestic pigeons in Switzerland and insight into population dynamics by Chlamydia psittaci multilocus sequence typing
    Article Snippet: Quantitative and conventional PCRs All quantitative PCRs (qPCR; , ) were run on an Applied Biosystems® 7500 Real-Time PCR System (Thermo Fisher Scientific). .. Products from all conventional PCRs ( , ) were purified using the QIAquick® PCR Purification Kit (Qiagen) according to the manufacturer’s instructions.

    Article Title: Long Non-coding RNA Maternally Expressed 3 Increases the Expression of Neuron-Specific Genes by Targeting miR-128-3p in All-Trans Retinoic Acid-Induced Neurogenic Differentiation From Amniotic Epithelial Cells
    Article Snippet: .. It was then reverse-transcribed and used for qPCR analysis with the RNA PCR kit version 3.0 (Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR system. ..

    Article Title: 9-ING-41, a small molecule inhibitor of GSK-3beta, potentiates the effects of anticancer therapeutics in bladder cancer
    Article Snippet: .. Real-time quantitative reverse transcriptase-PCR (RT-PCR) was done in the 7500 Real Time PCR System (Applied Biosystems, Waltham, MA) using pre-designed TaqMan Universal PCR Mastermix (Applied Biosystems, Waltham, MA) targeting human Bcl-2 and XIAP mRNA, and GAPDH was used as endogenous control . .. The expression of the target mRNA was quantified relative to that of the GAPDH mRNA .

    In Silico:

    Article Title: Loss of GAS5 tumour suppressor lncRNA: an independent molecular cancer biomarker for short-term relapse and progression in bladder cancer patients
    Article Snippet: Based on published sequences (NCBI Ref Seq: NR_002578.3 for GAS5 and NM_000194.2 for HPRT1 ) and according to in silico analysis, specific primers for GAS5 (F: 5′-CTTGCCTGGACCAGCTTAAT-3′, R: 5′-CAAGCCGACTCTCCATACCT-3′) and HPRT1 (F: 5′-TGGAAAGGGTGTTTATTCCTCAT, R: 5′-ATGTAATCCAGCAGGTCAGCAA-3′) were designed and used for the amplification of a 122 bp GAS5 -specific and a 151 bp HPRT1 -specific amplicon. .. The 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA) was used for the qPCR assays.

    Expressing:

    Article Title: Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice
    Article Snippet: Expression values were compared using the delta-delta-Ct method, FOS was normalized to GAPDH and microRNA-29c was normalized to U6. .. RT-PCR was performed by a 7500 real-time PCR system (Applied Biosystems, Life Technologies, USA) following a PCR cycle of denaturation at 94°C for 30 seconds, annealing at 60°C for all genes for 60 seconds, and extension at 72°C for 2 minutes for 40 cycles.

    Article Title: Glycemic Reduction Alters White Blood Cell Counts and Inflammatory Gene Expression in Diabetes
    Article Snippet: Paragraph title: 2.2.6. Gene expression analysis ... Quantity values, calculated from cycle threshold (CT ) values, were based on standard curves obtained for each gene in each sample using the Applied Biosystems 7500 Real-Time PCR System.

    Article Title: Loss of GAS5 tumour suppressor lncRNA: an independent molecular cancer biomarker for short-term relapse and progression in bladder cancer patients
    Article Snippet: Quantitative real-time PCR A SYBR-Green fluorescent-based quantitative real-time PCR (qPCR) assays was developed and applied to assess GAS5 expression. .. The 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA) was used for the qPCR assays.

    Article Title: Downregulation of CDH16 in Papillary Thyroid Cancer and Its Potential Molecular Mechanism Analysed by qRT-PCR, TCGA and in silico Analysis
    Article Snippet: .. We used ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan) for cDNA preparation. qRT-PCR analysis of CDH16 expression was performed in triplicate using the Thunderbird SYBR qPCR Mix (Toyobo, Osaka, Japan) in the Applied Biosystems 7500 Real-time PCR system (Applied biosystems, Foster City, CA). .. We set the expression of GAPDH as an internal control.

    Article Title: Long Non-coding RNA Maternally Expressed 3 Increases the Expression of Neuron-Specific Genes by Targeting miR-128-3p in All-Trans Retinoic Acid-Induced Neurogenic Differentiation From Amniotic Epithelial Cells
    Article Snippet: The expression of genes upstream and downstream of miR-128-3p [β III-tubulin, glial fibrillary acidic protein (GFAP ), Jagged 1 (JAG1 ), and MEG3 ] was also tested using RT-qPCR. .. It was then reverse-transcribed and used for qPCR analysis with the RNA PCR kit version 3.0 (Takara, Dalian, China) on an Applied Biosystems 7500 Real-Time PCR system.

    Article Title: 9-ING-41, a small molecule inhibitor of GSK-3beta, potentiates the effects of anticancer therapeutics in bladder cancer
    Article Snippet: Real-time quantitative reverse transcriptase-PCR (RT-PCR) was done in the 7500 Real Time PCR System (Applied Biosystems, Waltham, MA) using pre-designed TaqMan Universal PCR Mastermix (Applied Biosystems, Waltham, MA) targeting human Bcl-2 and XIAP mRNA, and GAPDH was used as endogenous control . .. The expression of the target mRNA was quantified relative to that of the GAPDH mRNA .

    Concentration Assay:

    Article Title: Downregulation of CDH16 in Papillary Thyroid Cancer and Its Potential Molecular Mechanism Analysed by qRT-PCR, TCGA and in silico Analysis
    Article Snippet: Detection of CDH16 Expression by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) We used TRIzol reagent (Life Technologies, Carlsbad, CA) to extract total RNA from the paired PTC tumors and noncancerous thyroid tissues according to the manufacturer’s indication, and then the RNA purity and concentration were measured by Plate reader Infinite 200 PRO (Tecan, Swiss). .. We used ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan) for cDNA preparation. qRT-PCR analysis of CDH16 expression was performed in triplicate using the Thunderbird SYBR qPCR Mix (Toyobo, Osaka, Japan) in the Applied Biosystems 7500 Real-time PCR system (Applied biosystems, Foster City, CA).

    Binding Assay:

    Article Title: Metformin Ameliorates Lipotoxic β-Cell Dysfunction through a Concentration-Dependent Dual Mechanism of Action
    Article Snippet: Real-time polymerase chain reaction (PCR) was performed using SYBR Premix Ex Taq reagents (TaKaRa, Shiga, Japan) and a 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA). .. The following primer sequences were used: activating transcription factor 4 (ATF4), 5′-CCTGAACAGCGAAGTGTTGG-3′ (forward), 5′-TGGAGAACCCATGAGGTTTCAA-3′ (reverse); C/EBP homologous protein (CHOP), 5′-CAACAGAGGTCACACGCACA-3′ (forward), 5′-TCTCCTTCATGCGTTGCTTC-3′ (reverse); glucose-regulated protein 94 (GRP94), 5′-AAACGGCAACACTTCGGTCAG-3′ (forward), 5′-GCATCCATCTCTTCTCCCTCATC-3′ (reverse); FK506 binding protein 11 (FKBP11), 5′-ACACGCTCCACATACACTACACGG-3′ (forward), 5′-ATGACTGCTCTTCGCTTCTCTCCC-3′ (reverse); NADPH oxidase (NOX) 1, 5′-AATGCCCAGGATCGAGGT-3′ (forward), 5′-GATGGAAGCAAAGGGAGTGA-3′ (reverse); NOX2, 5′-CCCTTTGGTACAGCCAGTGAAGAT-3′ (forward), 5′-CAATCCCGGCTCCCACTAACA-3′ (reverse).

    Plasmid Preparation:

    Article Title: Pituitary Action of E2 in Prepubertal Grass Carp: Receptor Specificity and Signal Transduction for Luteinizing Hormone and Follicle-Stimulating Hormone Regulation
    Article Snippet: The RT samples were subjected to qPCR using a 7500 real time PCR system (Applied Biosystems, USA) with primers specific for grass carp LHβ, FSHβ, and GREB1, respectively (see Table S1 in Supplementary Material for primer sequences and PCR condition). .. In these experiments, serial dilutions of plasmid DNA with the coding sequences for grass carp LHβ, FSHβ, and GREB1 were used as the standards for data calibration, and parallel real-time PCR for β-actin was also conducted to serve as the internal control.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Synergistic effects of Lactobacillus rhamnosus culture supernatant and bone marrow mesenchymal stem cells on the development of alcoholic steatohepatitis in mice
    Article Snippet: .. RT-PCR was performed by a 7500 real-time PCR system (Applied Biosystems, Life Technologies, USA) following a PCR cycle of denaturation at 94°C for 30 seconds, annealing at 60°C for all genes for 60 seconds, and extension at 72°C for 2 minutes for 40 cycles. .. RT-PCR was performed by a 7500 real-time PCR system (Applied Biosystems, Life Technologies, USA) following a PCR cycle of denaturation at 94°C for 30 seconds, annealing at 60°C for all genes for 60 seconds, and extension at 72°C for 2 minutes for 40 cycles.

    Article Title: 9-ING-41, a small molecule inhibitor of GSK-3beta, potentiates the effects of anticancer therapeutics in bladder cancer
    Article Snippet: .. Real-time quantitative reverse transcriptase-PCR (RT-PCR) was done in the 7500 Real Time PCR System (Applied Biosystems, Waltham, MA) using pre-designed TaqMan Universal PCR Mastermix (Applied Biosystems, Waltham, MA) targeting human Bcl-2 and XIAP mRNA, and GAPDH was used as endogenous control . .. The expression of the target mRNA was quantified relative to that of the GAPDH mRNA .

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  • 90
    Thermo Fisher abi 7500 fast dx
    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the <t>ABI</t> 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions
    Abi 7500 Fast Dx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi 7500 fast dx/product/Thermo Fisher
    Average 90 stars, based on 6 article reviews
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    abi 7500 fast dx - by Bioz Stars, 2020-02
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    90
    Thermo Fisher fast real time pcr system
    Gene expression analysis by RNA sequencing of LT-HSCs and MEPs. (A) Unsupervised clustering of differentially expressed genes (P ≤ 0.05; log2-fold changes > 1.5). Each column represents data from one individual mouse. The color code for the genotypes of the individual mice is the same as in B. (B) PCA. The data for LT-HSCs and MEPs were derived from two independent experiments and combined ( n = 4 or 5 for SclCre , n = 7 or 4 for SclCre;Ezh2 +/Δ , n = 4 for SclCre;Ezh2 Δ/Δ , n = 6 or 4 for SclCre;V617F , n = 8 or 6 SclCre;V617F;Ezh2 +/Δ , and n = 6 for SclCre;V617F;Ezh2 Δ/Δ for LT-HSCs or MEP analysis). Each dot represents data from one individual mouse. (C) Competitive gene set enrichment analysis for gene expression signatures of interferon-γ, interferon-α, and fetal liver HSCs in LT-HSCs of SclCre;V617F;Ezh2 Δ/Δ compared with SclCre;V617F . (D) Plot showing the number of differentially expressed genes with cutoff of P ≤ 0.05. (E) Gene list of top 10 significant gene expression differences according to the absolute fold change. (F) Expression levels of Lin28b and Hmga2 . Each dot represents data from one individual mouse. (G) Relative expression of HMGA2 , IGF2BP3 , and Pcolce2 determined by <t>qPCR</t> in granulocyte RNA from patients with MPN that carry mutations in EZH2 or CALR . Each dot represents data from one individual patient. The mutations in patient granulocyte were determined by allele-specific <t>PCR</t> ( n = 4 for CALR mutation with WT EZH2 , n = 4 for CALR mutation with heterozygous mutation of EZH2 , n = 1 for CALR mutation with homozygous mutation of EZH2 , n = 8 for JAK2V617F mutation with WT EZH2 , n = 12 for JAK2V617F mutation with heterozygous mutation of EZH2 , and n = 4 for JAK2V617F mutation with h homozygous mutation of EZH2 ). (F and G) Horizontal lines indicate the mean of the values. *, P
    Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast real time pcr system/product/Thermo Fisher
    Average 90 stars, based on 217 article reviews
    Price from $9.99 to $1999.99
    fast real time pcr system - by Bioz Stars, 2020-02
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    Tower Computer Kit for the Applied Biosystems 7500 and 7300 Real Time PCR Systems Monitor is not included Used With7500 Real Time PCR System with Dell Tower For Research Use
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    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: The performance of an alternative RT-PCR master mix, qScript™ One-Step qRT-PCR kit, Low Rox™ (Quanta) real-time RT-PCR master mix, was evaluated on the ABI 7500 Fast Dx and QuantStudio Dx instruments using the same RNA that was tested in the previous study.

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation

    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Article Snippet: The performance of an alternative RT-PCR master mix, qScript™ One-Step qRT-PCR kit, Low Rox™ (Quanta) real-time RT-PCR master mix, was evaluated on the ABI 7500 Fast Dx and QuantStudio Dx instruments using the same RNA that was tested in the previous study.

    Techniques: Lysis, Multiplex Assay

    Gene expression analysis by RNA sequencing of LT-HSCs and MEPs. (A) Unsupervised clustering of differentially expressed genes (P ≤ 0.05; log2-fold changes > 1.5). Each column represents data from one individual mouse. The color code for the genotypes of the individual mice is the same as in B. (B) PCA. The data for LT-HSCs and MEPs were derived from two independent experiments and combined ( n = 4 or 5 for SclCre , n = 7 or 4 for SclCre;Ezh2 +/Δ , n = 4 for SclCre;Ezh2 Δ/Δ , n = 6 or 4 for SclCre;V617F , n = 8 or 6 SclCre;V617F;Ezh2 +/Δ , and n = 6 for SclCre;V617F;Ezh2 Δ/Δ for LT-HSCs or MEP analysis). Each dot represents data from one individual mouse. (C) Competitive gene set enrichment analysis for gene expression signatures of interferon-γ, interferon-α, and fetal liver HSCs in LT-HSCs of SclCre;V617F;Ezh2 Δ/Δ compared with SclCre;V617F . (D) Plot showing the number of differentially expressed genes with cutoff of P ≤ 0.05. (E) Gene list of top 10 significant gene expression differences according to the absolute fold change. (F) Expression levels of Lin28b and Hmga2 . Each dot represents data from one individual mouse. (G) Relative expression of HMGA2 , IGF2BP3 , and Pcolce2 determined by qPCR in granulocyte RNA from patients with MPN that carry mutations in EZH2 or CALR . Each dot represents data from one individual patient. The mutations in patient granulocyte were determined by allele-specific PCR ( n = 4 for CALR mutation with WT EZH2 , n = 4 for CALR mutation with heterozygous mutation of EZH2 , n = 1 for CALR mutation with homozygous mutation of EZH2 , n = 8 for JAK2V617F mutation with WT EZH2 , n = 12 for JAK2V617F mutation with heterozygous mutation of EZH2 , and n = 4 for JAK2V617F mutation with h homozygous mutation of EZH2 ). (F and G) Horizontal lines indicate the mean of the values. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Loss of Ezh2 synergizes with JAK2-V617F in initiating myeloproliferative neoplasms and promoting myelofibrosis

    doi: 10.1084/jem.20151136

    Figure Lengend Snippet: Gene expression analysis by RNA sequencing of LT-HSCs and MEPs. (A) Unsupervised clustering of differentially expressed genes (P ≤ 0.05; log2-fold changes > 1.5). Each column represents data from one individual mouse. The color code for the genotypes of the individual mice is the same as in B. (B) PCA. The data for LT-HSCs and MEPs were derived from two independent experiments and combined ( n = 4 or 5 for SclCre , n = 7 or 4 for SclCre;Ezh2 +/Δ , n = 4 for SclCre;Ezh2 Δ/Δ , n = 6 or 4 for SclCre;V617F , n = 8 or 6 SclCre;V617F;Ezh2 +/Δ , and n = 6 for SclCre;V617F;Ezh2 Δ/Δ for LT-HSCs or MEP analysis). Each dot represents data from one individual mouse. (C) Competitive gene set enrichment analysis for gene expression signatures of interferon-γ, interferon-α, and fetal liver HSCs in LT-HSCs of SclCre;V617F;Ezh2 Δ/Δ compared with SclCre;V617F . (D) Plot showing the number of differentially expressed genes with cutoff of P ≤ 0.05. (E) Gene list of top 10 significant gene expression differences according to the absolute fold change. (F) Expression levels of Lin28b and Hmga2 . Each dot represents data from one individual mouse. (G) Relative expression of HMGA2 , IGF2BP3 , and Pcolce2 determined by qPCR in granulocyte RNA from patients with MPN that carry mutations in EZH2 or CALR . Each dot represents data from one individual patient. The mutations in patient granulocyte were determined by allele-specific PCR ( n = 4 for CALR mutation with WT EZH2 , n = 4 for CALR mutation with heterozygous mutation of EZH2 , n = 1 for CALR mutation with homozygous mutation of EZH2 , n = 8 for JAK2V617F mutation with WT EZH2 , n = 12 for JAK2V617F mutation with heterozygous mutation of EZH2 , and n = 4 for JAK2V617F mutation with h homozygous mutation of EZH2 ). (F and G) Horizontal lines indicate the mean of the values. *, P

    Article Snippet: Real-time qPCR was performed with 7500 Fast Real-Time PCR System, using the TaqMan gene expression system (Thermo Fisher Scientific) and normalized by human GUSB.

    Techniques: Expressing, RNA Sequencing Assay, Mouse Assay, Derivative Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Mutagenesis