7500 fast system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 7500 fast system
    7500 Fast System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7500 fast system/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7500 fast system - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Assessment of Real-Time PCR Assay for Detection of Rickettsia spp. and Rickettsia rickettsii in Banked Clinical Samples
    Article Snippet: Several real-time PCR assays have been developed for rickettsial agent detection, including Rickettsia genus-specific (panrickettsia) and R. rickettsii -specific assays ( – ). .. The assays were optimized on the Applied Biosystems 7500 FastDX (Life Technologies Corp., NY) instrument, which is widely available in U.S. public health laboratories, and their utility was validated with a large set of clinical samples submitted to the CDC for Rocky Mountain spotted fever testing.

    Article Title: Highly Efficient Large-Scale Lentiviral Vector Concentration by Tandem Tangential Flow Filtration
    Article Snippet: .. DELTRAN I disposable pressure transducer (Utah Medical Products, Midvale, UT, Cat No. DPT 100) 150 cm2 Tissue Culture Flasks (Corning, Corning, NY, Cat No. 430825) TripleFlask (NUNC, Rochester, NY, Cat No. 132867) HYPERflask (Corning, Corning, NY, Cat No. 10010) Syringe filters (Millipore, Cat No. SLGV033RS) 5 mL syringe (BD Medical, Franklin Lakes, NJ, Cat No. 309603) 0.8 μm Filter Units (Nalgene, Rochester, NY, Cat No. 127-0080) 0.22 μm Filter Units (Millipore, Billerica, MA, Cat No. SCGPU05RE) 2 mL Screw Cap Tubes (VWR, Radnor, PA, Cat No. 80078-428) 0.5 mL Screw Cap Tubes (VWR, Radnor, PA, Cat No. 89004-318) 2 mL Cryovials (Nalgene, Rochester, NY, Cat No. 5011-0020) PureLink Genomic DNA Mini Kit (Invitrogen, Carlsbad, CA, Cat No. K1820-01) MicroAmp Optical 96-Well Reaction Plate (Applied Biosystems, Cat No. N8010560) MicroAmp Optical Adhesive Film (Applied Biosystems, Carlsbad, CA, Cat No. 4311971) 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA, Cat No. 4362143) .. 293T cells (ATCC, Manassas, VA, Cat No. CRL-1268) HT-29 cells (ATCC, Manassas, VA, Cat No. CCL-218) TransIT-293 (Mirus, Madison, WI, Cat. No. MIR2706) Opti-MEM (Invitrogen, Carlsbad, CA, Cat. No. 31985-062) Dulbecco’s modified Eagle’s medium [DMEM] (Mediatech, Herndon, VA, Cat. No. 15-013- CV) Fetal Bovine Serum (Omega, Tarzana, CA, Cat. No. FB-01) L-Glutamine/Penicillin/Streptomycin (Gemini Bioproducts, Woodland, CA, Cat. No. 400110 100ML) Sodium Butyrate (Sigma-Aldrich, St. Louis, MO, Cat. No. B5887-5G) 1M HEPES (Invitrogen, Carlsbad, CA, Cat No. 15630-130) Dulbecco’s Phosphate Buffered Saline [DPBS] (Mediatech, Herndon, VA, Cat. No. 21-031- CV) UltraCULTURE (LONZA, Basel, Switzerland, Cat. No. 12-725F) 0.05% Trypsin EDTA 1X (Mediatech, Herndon, VA, Cat. No. 25-052-CI) 0.25% Trypsin-EDTA (Invitrogen, Carlsbad, CA, Cat No. 15050065) Genomic DNA Prepared Using NucleoBond Xtra Maxi EF Kit (Clontech, Cat No. 740424.10) Primers and Probes Ordered From Integrated DNA Technologies (IDT) (Coralville, IA) TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad, CA, Cat No. 4304437)

    Nested PCR:

    Article Title: Assessment of Real-Time PCR Assay for Detection of Rickettsia spp. and Rickettsia rickettsii in Banked Clinical Samples
    Article Snippet: A 2000-2007 national surveillance summary noted that < 0.5% of reported cases were diagnosed using nested PCR methodology ( ). .. The assays were optimized on the Applied Biosystems 7500 FastDX (Life Technologies Corp., NY) instrument, which is widely available in U.S. public health laboratories, and their utility was validated with a large set of clinical samples submitted to the CDC for Rocky Mountain spotted fever testing.

    Flow Cytometry:

    Article Title: Highly Efficient Large-Scale Lentiviral Vector Concentration by Tandem Tangential Flow Filtration
    Article Snippet: Flow Path 2 [FPII] (Spectrum labs, Rancho Dominguez, CA, Cat No. EZ-CHIL07-01-I) ■ The hollow fiber filter in FP2 contains 12 fibers (0.5 mm internal diameter) with a total surface area of 40 cm2 and a 500 kDa cut-off. .. DELTRAN I disposable pressure transducer (Utah Medical Products, Midvale, UT, Cat No. DPT 100) 150 cm2 Tissue Culture Flasks (Corning, Corning, NY, Cat No. 430825) TripleFlask (NUNC, Rochester, NY, Cat No. 132867) HYPERflask (Corning, Corning, NY, Cat No. 10010) Syringe filters (Millipore, Cat No. SLGV033RS) 5 mL syringe (BD Medical, Franklin Lakes, NJ, Cat No. 309603) 0.8 μm Filter Units (Nalgene, Rochester, NY, Cat No. 127-0080) 0.22 μm Filter Units (Millipore, Billerica, MA, Cat No. SCGPU05RE) 2 mL Screw Cap Tubes (VWR, Radnor, PA, Cat No. 80078-428) 0.5 mL Screw Cap Tubes (VWR, Radnor, PA, Cat No. 89004-318) 2 mL Cryovials (Nalgene, Rochester, NY, Cat No. 5011-0020) PureLink Genomic DNA Mini Kit (Invitrogen, Carlsbad, CA, Cat No. K1820-01) MicroAmp Optical 96-Well Reaction Plate (Applied Biosystems, Cat No. N8010560) MicroAmp Optical Adhesive Film (Applied Biosystems, Carlsbad, CA, Cat No. 4311971) 7500 Real-Time PCR System (Applied Biosystems, Carlsbad, CA, Cat No. 4362143)

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    Thermo Fisher abi 7500 fast dx
    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the <t>ABI</t> 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions
    Abi 7500 Fast Dx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi 7500 fast dx/product/Thermo Fisher
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    abi 7500 fast dx - by Bioz Stars, 2020-04
    90/100 stars
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    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: The performance of an alternative RT-PCR master mix, qScript™ One-Step qRT-PCR kit, Low Rox™ (Quanta) real-time RT-PCR master mix, was evaluated on the ABI 7500 Fast Dx and QuantStudio Dx instruments using the same RNA that was tested in the previous study.

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation

    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Article Snippet: The performance of an alternative RT-PCR master mix, qScript™ One-Step qRT-PCR kit, Low Rox™ (Quanta) real-time RT-PCR master mix, was evaluated on the ABI 7500 Fast Dx and QuantStudio Dx instruments using the same RNA that was tested in the previous study.

    Techniques: Lysis, Multiplex Assay

    Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Clinical performance of the Trioplex assay across specimen types. Clinical specimens collected concurrently from 373 cases with previous Zika determination in the acute stage were tested. RNA was extracted with the MagNA Pure 96 small volume external lysis protocol from 373 case-paired serum, 373 urine, and 345 whole blood-EDTA specimens and tested with the Trioplex assay in multiplex format in the ABI 7500 Fast Dx instrument. a Correlation of CT values between case-matching serum and urine specimens; R 2 = 0.36 p

    Article Snippet: Performance of the Trioplex assay was evaluated using RNA extractions from the MagNA Pure LC 2.0, MagNA Pure 96, MagNA Pure Compact, and NucliSENS® easyMag® platforms and run on the ABI 7500 Fast Dx and QuantStudio Dx real-time PCR instruments.

    Techniques: Lysis, Multiplex Assay