7500 fast real time pcr system (Thermo Fisher)
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Thermo Fisher
7500 fast real time pcr system
7500 Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/7500 fast real time pcr system/product/Thermo Fisher
Average 99 stars, based on 1800 article reviews
Price from $9.99 to $1999.99
7500 Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/7500 fast real time pcr system/product/Thermo Fisher
Average 99 stars, based on 1800 article reviews
Price from $9.99 to $1999.99
7500 fast real time pcr system - by Bioz Stars,
2020-01
99/100 stars
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Clone Assay:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: HRM data were generated using the Applied Biosystems Amplification:Article Title: A Paradigm of Endothelium-Protective and Stent-Free Anti-Restenotic Therapy Using Biomimetic Nanoclusters Article Snippet: Purified mRNA (1 μg) was used for the first-strand cDNA synthesis and quantitative RT-PCR was performed using the Article Title: The dual role of the centrosome in organizing the microtubule network in interphase Article Snippet: .. Optimization and amplification of each specific gene product were performed using the Applied Biosystems Article Title: Histone methylation regulator PTIP is required to maintain normal and leukemic bone marrow niches Article Snippet: One microgram of total RNA was treated with amplification-grade DNaseI (Qiagen) and was reverse-transcribed with the SuperScript III SuperMix system (Invitrogen), according to the manufacturers’ protocols. mRNA levels were analyzed via RT-qPCR analysis using the iTaq SYBR Green Supermix (Bio-Rad Laboratories), according to the manufacturer’s protocol. .. Reactions were performed on an ABI 7900HT Fast Real-Time PCR system or a Synthesized:Article Title: Increased interleukin-26 expression in proliferative diabetic retinopathy Article Snippet: RNA was abstracted from the PBMCs with Trizol Reagent (Takara, Japan) complied with the manufacturer's instruction. cDNA was synthesized using Superscript III Reverse Transcriptase (Takara, Japan) and then the synthesized first-strand of cDNA was measured by real-time quantitative PCR analysis with SYBR Green labeling method. .. Quantitative PCR was conducted by an Applied Biosystems Article Title: The dual role of the centrosome in organizing the microtubule network in interphase Article Snippet: Complementary DNA (cDNA) was synthesized with 3 μg of total RNA using random hexamers and SuperScript III Reverse Transcriptase (Invitrogen™). cDNAs were diluted in sterile water and used as template for the amplification by the PCR. .. Optimization and amplification of each specific gene product were performed using the Applied Biosystems Article Title: Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling Article Snippet: Total RNA extraction and DNase treatment were performed following the manufacturer’s instructions of the RNeasy kit (Qiagen). cDNA was synthesized using iScript kit (BioRad) using 500 ng to 1 μg of RNA. cDNA was diluted 1:10 and 2 μl were used in each RT-qPCR reaction (technical triplicates), with 10 μl of iTAQ Universal SYBR Green Supermix (BioRad) and 400 nM of primers forward and reverse diluted in 8 μl of water. .. Runs were performed in a Article Title: Terpene profiling, transcriptome analysis and characterization of cis-ò-terpineol synthase from Ocimum Article Snippet: For semi-quantitative PCR, 2 µg DNase-treated RNA, each of young and mature leaves were mixed together and cDNA was synthesized as described. .. For qRT–PCR reactions, elongation factor-1 (EF1) was used as an endogenous control and the reactions were carried out in triplicates for 3 biological replicates in the TA Cloning:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: HRM data were generated using the Applied Biosystems Quantitative RT-PCR:Article Title: A Paradigm of Endothelium-Protective and Stent-Free Anti-Restenotic Therapy Using Biomimetic Nanoclusters Article Snippet: .. Purified mRNA (1 μg) was used for the first-strand cDNA synthesis and quantitative RT-PCR was performed using the Article Title: Increased interleukin-26 expression in proliferative diabetic retinopathy Article Snippet: Paragraph title: Real-time Quantitative RT-PCR ... Quantitative PCR was conducted by an Applied Biosystems Article Title: Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling Article Snippet: Paragraph title: Reverse transcription polymerase chain reaction (RT-qPCR) ... Runs were performed in a Article Title: Thyroid hormone receptor ò1 stimulates ABCB4 to increase biliary phosphatidylcholine excretion in mice Article Snippet: .. Quantitative real-time RT-PCR (qRT-PCR) was performed on a SYBR Green PCR Master Mix and with the Article Title: Fibromodulin Is Essential for Fetal-Type Scarless Cutaneous Wound Healing Article Snippet: .. Expression of mRNA was measured by real-time quantitative RT-PCR using TaqMan Gene Expression Assays on a Article Title: Histone methylation regulator PTIP is required to maintain normal and leukemic bone marrow niches Article Snippet: Paragraph title: RT-qPCR. ... Reactions were performed on an ABI 7900HT Fast Real-Time PCR system or a Article Title: Angiopoietin/Tie2 Axis Regulates the Age-at-Injury Cerebrovascular Response to Traumatic Brain Injury Article Snippet: .. The reverse transcription was performed by incubating the reaction mixes at 16°C for 30 min; 42°C for 30 min; 85°C for 5 min. qRT-PCR was performed in 18 μl of qRT-PCR mix (6.9 μl of water, 1.2 μl of miRNA-specific RT products, 0.9 μl of 20× miRNA-specific TaqMan small RNA assay mix, 9 μl of 2× TaqMan Fast Universal PCR Master Mix) on a Article Title: Tributyltin induces a transcriptional response without a brite adipocyte signature in adipocyte models Article Snippet: For RT-qPCR analyses, cDNA was prepared from total RNA using the GoScript™ Reverse Transcription System (Promega), with a 1:1 mixture of random and Oligo (dT)15 primers. .. The qPCR reactions were performed using a Article Title: Terpene profiling, transcriptome analysis and characterization of cis-ò-terpineol synthase from Ocimum Article Snippet: .. For qRT–PCR reactions, elongation factor-1 (EF1) was used as an endogenous control and the reactions were carried out in triplicates for 3 biological replicates in the SYBR Green Assay:Article Title: A Paradigm of Endothelium-Protective and Stent-Free Anti-Restenotic Therapy Using Biomimetic Nanoclusters Article Snippet: Purified mRNA (1 μg) was used for the first-strand cDNA synthesis and quantitative RT-PCR was performed using the Article Title: Increased interleukin-26 expression in proliferative diabetic retinopathy Article Snippet: RNA was abstracted from the PBMCs with Trizol Reagent (Takara, Japan) complied with the manufacturer's instruction. cDNA was synthesized using Superscript III Reverse Transcriptase (Takara, Japan) and then the synthesized first-strand of cDNA was measured by real-time quantitative PCR analysis with SYBR Green labeling method. .. Quantitative PCR was conducted by an Applied Biosystems Article Title: Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling Article Snippet: Total RNA extraction and DNase treatment were performed following the manufacturer’s instructions of the RNeasy kit (Qiagen). cDNA was synthesized using iScript kit (BioRad) using 500 ng to 1 μg of RNA. cDNA was diluted 1:10 and 2 μl were used in each RT-qPCR reaction (technical triplicates), with 10 μl of iTAQ Universal SYBR Green Supermix (BioRad) and 400 nM of primers forward and reverse diluted in 8 μl of water. .. Runs were performed in a Article Title: Thyroid hormone receptor ò1 stimulates ABCB4 to increase biliary phosphatidylcholine excretion in mice Article Snippet: .. Quantitative real-time RT-PCR (qRT-PCR) was performed on a SYBR Green PCR Master Mix and with the Article Title: Histone methylation regulator PTIP is required to maintain normal and leukemic bone marrow niches Article Snippet: One microgram of total RNA was treated with amplification-grade DNaseI (Qiagen) and was reverse-transcribed with the SuperScript III SuperMix system (Invitrogen), according to the manufacturers’ protocols. mRNA levels were analyzed via RT-qPCR analysis using the iTaq SYBR Green Supermix (Bio-Rad Laboratories), according to the manufacturer’s protocol. .. Reactions were performed on an ABI 7900HT Fast Real-Time PCR system or a Article Title: Terpene profiling, transcriptome analysis and characterization of cis-ò-terpineol synthase from Ocimum Article Snippet: A typical reaction consisted of 5 µL of SYBR Green master-mix, 0.5 µL each of forward and reverse primer (10 µM) and 1 µL of diluted cDNA (1:2) with nuclease-free water added to make up a volume of 10 µL. .. For qRT–PCR reactions, elongation factor-1 (EF1) was used as an endogenous control and the reactions were carried out in triplicates for 3 biological replicates in the Microarray:Article Title: Tributyltin induces a transcriptional response without a brite adipocyte signature in adipocyte models Article Snippet: Microarray analyses were performed by the Boston University Microarray and Sequencing Resource using GeneChip® Mouse Gene 2.0ST arrays (Affymetrix, Santa Clara, CA). .. The qPCR reactions were performed using a Incubation:Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: PCR was conducted using the TALENs:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: The first step in our analyses of TALEN effectiveness was to determine whether TALENs induced somatic mutations in injected embryos (F0 generation). .. HRM data were generated using the Applied Biosystems Expressing:Article Title: Deficit in PINK1/PARKIN-mediated mitochondrial autophagy at late stages of dystrophic cardiomyopathy Article Snippet: .. Real-time PCR was performed using a Article Title: Increased interleukin-26 expression in proliferative diabetic retinopathy Article Snippet: Quantitative PCR was conducted by an Applied Biosystems Article Title: The dual role of the centrosome in organizing the microtubule network in interphase Article Snippet: Optimization and amplification of each specific gene product were performed using the Applied Biosystems Article Title: Thyroid hormone receptor ò1 stimulates ABCB4 to increase biliary phosphatidylcholine excretion in mice Article Snippet: Quantitative real-time RT-PCR (qRT-PCR) was performed on a SYBR Green PCR Master Mix and with the Article Title: Fibromodulin Is Essential for Fetal-Type Scarless Cutaneous Wound Healing Article Snippet: .. Expression of mRNA was measured by real-time quantitative RT-PCR using TaqMan Gene Expression Assays on a Article Title: Angiopoietin/Tie2 Axis Regulates the Age-at-Injury Cerebrovascular Response to Traumatic Brain Injury Article Snippet: Paragraph title: Quantification of miRNA expression. ... The reverse transcription was performed by incubating the reaction mixes at 16°C for 30 min; 42°C for 30 min; 85°C for 5 min. qRT-PCR was performed in 18 μl of qRT-PCR mix (6.9 μl of water, 1.2 μl of miRNA-specific RT products, 0.9 μl of 20× miRNA-specific TaqMan small RNA assay mix, 9 μl of 2× TaqMan Fast Universal PCR Master Mix) on a Article Title: Tributyltin induces a transcriptional response without a brite adipocyte signature in adipocyte models Article Snippet: Paragraph title: mRNA Expression. ... The qPCR reactions were performed using a Modification:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: For detection of both somatic and germline-transmitted mutations in embryos, genomic DNA was isolated from pools of test fish (injected F0 embryos for detection of somatic mutations or offspring of F0 embryos for detection of germline-transmitted mutations) or un-injected WT AB strain fish at either 24 hpf or 3 dpf using a protocol modified from Ref. . .. HRM data were generated using the Applied Biosystems Concentration Assay:Article Title: Intestinal epithelial cell-specific Raptor is essential for high fat diet-induced weight gain in mice Article Snippet: NanoDrop Spectrophotometer (ND-1000; NanoDrop Technologies, Wilmington, DE) was used to determine RNA concentration of samples. .. RNA expression was determined with the Applied Biosystems TaqMan microRNA Assay:Article Title: Angiopoietin/Tie2 Axis Regulates the Age-at-Injury Cerebrovascular Response to Traumatic Brain Injury Article Snippet: As we described previously ( , ), the TaqMan miRNA assay kit (Thermo Fisher Scientific) was used to quantify miRNA expression per the manufacturer's instructions. .. The reverse transcription was performed by incubating the reaction mixes at 16°C for 30 min; 42°C for 30 min; 85°C for 5 min. qRT-PCR was performed in 18 μl of qRT-PCR mix (6.9 μl of water, 1.2 μl of miRNA-specific RT products, 0.9 μl of 20× miRNA-specific TaqMan small RNA assay mix, 9 μl of 2× TaqMan Fast Universal PCR Master Mix) on a Generated:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: .. HRM data were generated using the Applied Biosystems Polymerase Chain Reaction:Article Title: Deficit in PINK1/PARKIN-mediated mitochondrial autophagy at late stages of dystrophic cardiomyopathy Article Snippet: Paragraph title: 2.6. RNA isolation and real-time reverse transcription PCR ... Real-time PCR was performed using a Article Title: A Paradigm of Endothelium-Protective and Stent-Free Anti-Restenotic Therapy Using Biomimetic Nanoclusters Article Snippet: Purified mRNA (1 μg) was used for the first-strand cDNA synthesis and quantitative RT-PCR was performed using the Article Title: The dual role of the centrosome in organizing the microtubule network in interphase Article Snippet: Complementary DNA (cDNA) was synthesized with 3 μg of total RNA using random hexamers and SuperScript III Reverse Transcriptase (Invitrogen™). cDNAs were diluted in sterile water and used as template for the amplification by the PCR. .. Optimization and amplification of each specific gene product were performed using the Applied Biosystems Article Title: Thyroid hormone receptor ò1 stimulates ABCB4 to increase biliary phosphatidylcholine excretion in mice Article Snippet: .. Quantitative real-time RT-PCR (qRT-PCR) was performed on a SYBR Green PCR Master Mix and with the Article Title: Angiopoietin/Tie2 Axis Regulates the Age-at-Injury Cerebrovascular Response to Traumatic Brain Injury Article Snippet: .. The reverse transcription was performed by incubating the reaction mixes at 16°C for 30 min; 42°C for 30 min; 85°C for 5 min. qRT-PCR was performed in 18 μl of qRT-PCR mix (6.9 μl of water, 1.2 μl of miRNA-specific RT products, 0.9 μl of 20× miRNA-specific TaqMan small RNA assay mix, 9 μl of 2× TaqMan Fast Universal PCR Master Mix) on a Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: .. PCR was conducted using the Transmission Assay:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: F0 fish were then bred, and pools of F1 generation embryos were screened for successful germline transmission of TALEN-induced mutations. .. HRM data were generated using the Applied Biosystems Reverse Transcription Polymerase Chain Reaction:Article Title: Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling Article Snippet: Paragraph title: Reverse transcription polymerase chain reaction (RT-qPCR) ... Runs were performed in a Article Title: Terpene profiling, transcriptome analysis and characterization of cis-ò-terpineol synthase from Ocimum Article Snippet: Semi-quantitative RT–PCR (sqRT–PCR) was performed for all three TPS s in 10 µL reaction consisting of 5 µL of 2X Jumpstart readymix, 1 µL each of 10 µM gene specific forward and reverse primers, 1 µL of cDNA optimized with endogenous control (18S rRNA) and nuclease free water was added to make up a volume of 10 µL. .. For qRT–PCR reactions, elongation factor-1 (EF1) was used as an endogenous control and the reactions were carried out in triplicates for 3 biological replicates in the Injection:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: For detection of both somatic and germline-transmitted mutations in embryos, genomic DNA was isolated from pools of test fish (injected F0 embryos for detection of somatic mutations or offspring of F0 embryos for detection of germline-transmitted mutations) or un-injected WT AB strain fish at either 24 hpf or 3 dpf using a protocol modified from Ref. . .. HRM data were generated using the Applied Biosystems Isolation:Article Title: Deficit in PINK1/PARKIN-mediated mitochondrial autophagy at late stages of dystrophic cardiomyopathy Article Snippet: Paragraph title: 2.6. RNA isolation and real-time reverse transcription PCR ... Real-time PCR was performed using a Article Title: A Paradigm of Endothelium-Protective and Stent-Free Anti-Restenotic Therapy Using Biomimetic Nanoclusters Article Snippet: mRNA was isolated from collected carotid segments using TRIzol following the manufacturer’s instructions. .. Purified mRNA (1 μg) was used for the first-strand cDNA synthesis and quantitative RT-PCR was performed using the Article Title: The dual role of the centrosome in organizing the microtubule network in interphase Article Snippet: Paragraph title: RNA isolation, retro‐transcription, and real‐time PCR ... Optimization and amplification of each specific gene product were performed using the Applied Biosystems Article Title: Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling Article Snippet: For mRNA isolation, 5x105 cells were seeded in 6-well plate. .. Runs were performed in a Article Title: Thyroid hormone receptor ò1 stimulates ABCB4 to increase biliary phosphatidylcholine excretion in mice Article Snippet: Paragraph title: RNA isolation and qRT-PCR analysis ... Quantitative real-time RT-PCR (qRT-PCR) was performed on a SYBR Green PCR Master Mix and with the Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: For detection of both somatic and germline-transmitted mutations in embryos, genomic DNA was isolated from pools of test fish (injected F0 embryos for detection of somatic mutations or offspring of F0 embryos for detection of germline-transmitted mutations) or un-injected WT AB strain fish at either 24 hpf or 3 dpf using a protocol modified from Ref. . .. HRM data were generated using the Applied Biosystems Article Title: Fibromodulin Is Essential for Fetal-Type Scarless Cutaneous Wound Healing Article Snippet: Total RNA was isolated using the RNeasy FFPE Kit (Qiagen). .. Expression of mRNA was measured by real-time quantitative RT-PCR using TaqMan Gene Expression Assays on a Article Title: Histone methylation regulator PTIP is required to maintain normal and leukemic bone marrow niches Article Snippet: Cells were spun down and washed once with cold PBS; then RNA was isolated with a Qiagen RNeasy kit (Qiagen) according to the manufacturer’s protocol. .. Reactions were performed on an ABI 7900HT Fast Real-Time PCR system or a Article Title: Angiopoietin/Tie2 Axis Regulates the Age-at-Injury Cerebrovascular Response to Traumatic Brain Injury Article Snippet: Briefly, 16.5 ng (3.3 μl) total RNA, isolated as described above, was reverse transcribed using the TaqMan MicroRNA Reverse Transcription kit (Thermo Fisher Scientific) with miRNA-specific RT primers in 10 μl of reverse transcription mix (2.8 μl of water, 1 μl of 10× RT buffer, 0.1 μl of 100 m m dNTPs, 0.13 μl of RNase inhibitor at 20 U/ μl), 0.67 μl of MutiScribe reverse transcriptase at 50 U/μl, and 2 μl of 5× RT primers). .. The reverse transcription was performed by incubating the reaction mixes at 16°C for 30 min; 42°C for 30 min; 85°C for 5 min. qRT-PCR was performed in 18 μl of qRT-PCR mix (6.9 μl of water, 1.2 μl of miRNA-specific RT products, 0.9 μl of 20× miRNA-specific TaqMan small RNA assay mix, 9 μl of 2× TaqMan Fast Universal PCR Master Mix) on a Detection Assay:Article Title: Deficit in PINK1/PARKIN-mediated mitochondrial autophagy at late stages of dystrophic cardiomyopathy Article Snippet: .. Real-time PCR was performed using a Size-exclusion Chromatography:Article Title: Tributyltin induces a transcriptional response without a brite adipocyte signature in adipocyte models Article Snippet: .. The qPCR reactions were performed using a Labeling:Article Title: Increased interleukin-26 expression in proliferative diabetic retinopathy Article Snippet: RNA was abstracted from the PBMCs with Trizol Reagent (Takara, Japan) complied with the manufacturer's instruction. cDNA was synthesized using Superscript III Reverse Transcriptase (Takara, Japan) and then the synthesized first-strand of cDNA was measured by real-time quantitative PCR analysis with SYBR Green labeling method. .. Quantitative PCR was conducted by an Applied Biosystems Purification:Article Title: A Paradigm of Endothelium-Protective and Stent-Free Anti-Restenotic Therapy Using Biomimetic Nanoclusters Article Snippet: .. Purified mRNA (1 μg) was used for the first-strand cDNA synthesis and quantitative RT-PCR was performed using the Sequencing:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: HRM data were generated using the Applied Biosystems Article Title: Tributyltin induces a transcriptional response without a brite adipocyte signature in adipocyte models Article Snippet: Microarray analyses were performed by the Boston University Microarray and Sequencing Resource using GeneChip® Mouse Gene 2.0ST arrays (Affymetrix, Santa Clara, CA). .. The qPCR reactions were performed using a Chloramphenicol Acetyltransferase Assay:Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: DNA was extracted using a QIAamp DNA Mini Kit (Qiagen cat51304; Qiagen, Hilden, Germany) according to the manufacturer's instructions. .. PCR was conducted using the RNA Extraction:Article Title: Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling Article Snippet: Total RNA extraction and DNase treatment were performed following the manufacturer’s instructions of the RNeasy kit (Qiagen). cDNA was synthesized using iScript kit (BioRad) using 500 ng to 1 μg of RNA. cDNA was diluted 1:10 and 2 μl were used in each RT-qPCR reaction (technical triplicates), with 10 μl of iTAQ Universal SYBR Green Supermix (BioRad) and 400 nM of primers forward and reverse diluted in 8 μl of water. .. Runs were performed in a Plasmid Preparation:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: HRM data were generated using the Applied Biosystems Real-time Polymerase Chain Reaction:Article Title: Deficit in PINK1/PARKIN-mediated mitochondrial autophagy at late stages of dystrophic cardiomyopathy Article Snippet: .. Real-time PCR was performed using a Article Title: A Paradigm of Endothelium-Protective and Stent-Free Anti-Restenotic Therapy Using Biomimetic Nanoclusters Article Snippet: .. Purified mRNA (1 μg) was used for the first-strand cDNA synthesis and quantitative RT-PCR was performed using the Article Title: Pigment Visibility on Rectal Swabs Used To Detect Enteropathogens: a Prospective Cohort Study Article Snippet: .. The reaction was performed using a Article Title: Increased interleukin-26 expression in proliferative diabetic retinopathy Article Snippet: .. Quantitative PCR was conducted by an Applied Biosystems Article Title: The dual role of the centrosome in organizing the microtubule network in interphase Article Snippet: .. Optimization and amplification of each specific gene product were performed using the Applied Biosystems Article Title: Hypoxic cancer-associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling Article Snippet: .. Runs were performed in a Article Title: Thyroid hormone receptor ò1 stimulates ABCB4 to increase biliary phosphatidylcholine excretion in mice Article Snippet: .. Quantitative real-time RT-PCR (qRT-PCR) was performed on a SYBR Green PCR Master Mix and with the Article Title: Intestinal epithelial cell-specific Raptor is essential for high fat diet-induced weight gain in mice Article Snippet: .. RNA expression was determined with the Applied Biosystems Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: .. HRM data were generated using the Applied Biosystems Article Title: Fibromodulin Is Essential for Fetal-Type Scarless Cutaneous Wound Healing Article Snippet: .. Expression of mRNA was measured by real-time quantitative RT-PCR using TaqMan Gene Expression Assays on a Article Title: Histone methylation regulator PTIP is required to maintain normal and leukemic bone marrow niches Article Snippet: .. Reactions were performed on an ABI 7900HT Fast Real-Time PCR system or a Article Title: Angiopoietin/Tie2 Axis Regulates the Age-at-Injury Cerebrovascular Response to Traumatic Brain Injury Article Snippet: .. The reverse transcription was performed by incubating the reaction mixes at 16°C for 30 min; 42°C for 30 min; 85°C for 5 min. qRT-PCR was performed in 18 μl of qRT-PCR mix (6.9 μl of water, 1.2 μl of miRNA-specific RT products, 0.9 μl of 20× miRNA-specific TaqMan small RNA assay mix, 9 μl of 2× TaqMan Fast Universal PCR Master Mix) on a Article Title: Tributyltin induces a transcriptional response without a brite adipocyte signature in adipocyte models Article Snippet: .. The qPCR reactions were performed using a Article Title: Terpene profiling, transcriptome analysis and characterization of cis-ò-terpineol synthase from Ocimum Article Snippet: .. For qRT–PCR reactions, elongation factor-1 (EF1) was used as an endogenous control and the reactions were carried out in triplicates for 3 biological replicates in the Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: .. PCR was conducted using the Multiplex Assay:Article Title: Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens, et al. Comparison between DiaPlexQ™ STI6 and GeneFinder™ STD I/STD II multiplex Real‐time PCR Kits in the detection of six sexually transmitted disease pathogens Article Snippet: For the DiaPlexQ assay, extracted DNA (5 μL) was added to a tube containing 15 μL of PCR premix (10 μL of 2X Multiplex Real‐Time PCR Smart mix and 5 μL of Primer & Probe Mixture). .. PCR was conducted using the RNA Expression:Article Title: Intestinal epithelial cell-specific Raptor is essential for high fat diet-induced weight gain in mice Article Snippet: .. RNA expression was determined with the Applied Biosystems Laser Capture Microdissection:Article Title: Fibromodulin Is Essential for Fetal-Type Scarless Cutaneous Wound Healing Article Snippet: Total RNA of unwounded E16 and E18 fetal rat skin was isolated using the RNeasy Mini Kit with DNase treatment (Qiagen, Valencia, CA), while wound tissues were collected by microdissection from tissue sections. .. Expression of mRNA was measured by real-time quantitative RT-PCR using TaqMan Gene Expression Assays on a Spectrophotometry:Article Title: Deficit in PINK1/PARKIN-mediated mitochondrial autophagy at late stages of dystrophic cardiomyopathy Article Snippet: Total RNA was isolated using Tri-Reagent® and Direct-zol RNA MiniPrep kit according to manufacturer’s instructions (Zymo Research, Irvine, CA) and quantified with a Nanodrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE). .. Real-time PCR was performed using a Article Title: Intestinal epithelial cell-specific Raptor is essential for high fat diet-induced weight gain in mice Article Snippet: NanoDrop Spectrophotometer (ND-1000; NanoDrop Technologies, Wilmington, DE) was used to determine RNA concentration of samples. .. RNA expression was determined with the Applied Biosystems Activation Assay:Article Title: Tributyltin induces a transcriptional response without a brite adipocyte signature in adipocyte models Article Snippet: .. The qPCR reactions were performed using a Formalin-fixed Paraffin-Embedded:Article Title: Fibromodulin Is Essential for Fetal-Type Scarless Cutaneous Wound Healing Article Snippet: Total RNA was isolated using the RNeasy FFPE Kit (Qiagen). .. Expression of mRNA was measured by real-time quantitative RT-PCR using TaqMan Gene Expression Assays on a Fluorescence In Situ Hybridization:Article Title: Prion gene paralogs are dispensable for early zebrafish development and have nonadditive roles in seizure susceptibility Article Snippet: For detection of both somatic and germline-transmitted mutations in embryos, genomic DNA was isolated from pools of test fish (injected F0 embryos for detection of somatic mutations or offspring of F0 embryos for detection of germline-transmitted mutations) or un-injected WT AB strain fish at either 24 hpf or 3 dpf using a protocol modified from Ref. . .. HRM data were generated using the Applied Biosystems |