cgmcc 1 7461  (ATCC)

Journal: Oncoimmunology

Article Title: T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer

doi: 10.1080/2162402X.2021.1940675

Figure Lengend Snippet:

Article Snippet: Hyaluronidase , Stem Cell technologies , Stem cell technologies Cat # 7461.

Techniques: Purification, Control, Activation Assay, Blocking Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Cell Stimulation, Red Blood Cell Lysis

Journal: Molecular Therapy

Article Title: Cyclic Peptides to Improve Delivery and Exon Skipping of Antisense Oligonucleotides in a Mouse Model for Duchenne Muscular Dystrophy

doi: 10.1016/j.ymthe.2017.10.004

Figure Lengend Snippet: Evaluation of CyPep10-AON Conjugate CyPep10 was conjugated to an AON targeting human dystrophin exon 45 (h45AON and CyPep10-h45AON) to evaluate whether the conjugation of a cyclic peptide has any influence on the exon-skipping functionality of the AON. (A) Human control myotubes incubated with 2 or 4 μM AON without any transfection reagent for 96 hr. Results show the average exon-skipping levels of two independent experiments in duplo. (B) IM injection in two hDMD mice (pretreated with cardiotoxin) with 20 μg of h45AON or molar equivalent of CyPep10-h45AON for 2 consecutive days. Results represent an average of two independent experiments in duplo. Bars represent mean ± SD. G, gastrocnemius; Tr, triceps.

Article Snippet: Subsequently, slides were stained overnight at 4°C with primary antibody (Dystrophin antibody, 1:50 [Santa Cruz, Germany]; sc-7461; Laminin antibody, 1:100 [Abcam, UK]; ab11575) in blocking buffer (PBS/0.05% Tween/5% horse serum).

Techniques: Conjugation Assay, Control, Incubation, Transfection, Injection

Journal: Molecular Therapy

Article Title: Cyclic Peptides to Improve Delivery and Exon Skipping of Antisense Oligonucleotides in a Mouse Model for Duchenne Muscular Dystrophy

doi: 10.1016/j.ymthe.2017.10.004

Figure Lengend Snippet: Dystrophin Analysis (A and B) Dystrophin protein levels have been determined (A) in quadriceps muscle by western blot and (B) by immunofluorescent staining of dystrophin in triceps muscle. The first four lanes in (A) named 12.5%–0.6% are diluted control protein lysates. M, marker.

Article Snippet: Subsequently, slides were stained overnight at 4°C with primary antibody (Dystrophin antibody, 1:50 [Santa Cruz, Germany]; sc-7461; Laminin antibody, 1:100 [Abcam, UK]; ab11575) in blocking buffer (PBS/0.05% Tween/5% horse serum).

Techniques: Western Blot, Staining, Control, Marker