74004 rneasy micro kit  (Qiagen)


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    Name:
    RNeasy Micro Kit
    Description:
    For purification of up to 45 µg total RNA from cell and tissue samples Kit contents Qiagen RNeasy Micro Kit 50 preps 10 to 14L Elution Volume 5mg Sample Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes 50 RNeasy MinElute Spin Columns Collection Tubes 1 5mL and 2mL RNase free DNase I Carrier RNA RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from very small amounts of starting material No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation
    Catalog Number:
    74004
    Price:
    492
    Category:
    RNeasy Micro Kit
    Buy from Supplier


    Structured Review

    Qiagen 74004 rneasy micro kit
    RNeasy Micro Kit
    For purification of up to 45 µg total RNA from cell and tissue samples Kit contents Qiagen RNeasy Micro Kit 50 preps 10 to 14L Elution Volume 5mg Sample Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes 50 RNeasy MinElute Spin Columns Collection Tubes 1 5mL and 2mL RNase free DNase I Carrier RNA RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from very small amounts of starting material No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation
    https://www.bioz.com/result/74004 rneasy micro kit/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    74004 rneasy micro kit - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Transduction:

    Article Title: Myeloid lineage enhancers drive oncogene synergy in CEBPA/CSF3R mutant acute myeloid leukemia
    Article Snippet: RNA sequencing Bone marrow from C57BL/6 mice was harvested and retrovirally transduced with the indicated oncogene combinations as described above. .. RNA was extracted using the RNeasy micro kit (Qiagen). cDNA libraries were constructed using the SMARTer universal low input RNA kit (Clonetech) and sequenced using a HiSeq 2500 Sequencer (Illumina) 100 bp SR.

    Centrifugation:

    Article Title: Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition
    Article Snippet: .. One volume of 70% EtOH was added to the lysate, transferred to an RNeasy MinElute spin column, and centrifuged for 15 s. Next, 350 μl of buffer RW1 from QIAGEN RNeasy Micro Kit was added to wash the RNeasy MinElute spin column by 15 s centrifugation. .. 10 μl DNase I mix was added to the RNeasy MinElute spin column membrane (10 μl DNase + 70 μl buffer RDD) and incubated at room temperature for 15 min.

    Amplification:

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: RNA extraction was undertaken with a Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA), and quality was assessed using an Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, CA). .. Following the manufacturer's instructions, three rounds of linear amplification of the target was carried out using the MessageAmp aRNA Amplification kit (Ambion, Austin, TX).

    Article Title: Role of cell signaling in poxvirus-mediated foreign gene expression in mammalian cells
    Article Snippet: Total RNA was isolated from virus-infected or uninfected cells by use of RNeasy Micro Kit (QIAGEN, Valencia, CA) following the manufacturer's protocol. .. Both DNA and total RNA samples were quantified spectrophotopically prior to PCR amplification.

    Synthesized:

    Article Title: The role of receptor MAS in microglia-driven retinal vascular development
    Article Snippet: Quantitative PCR RNA was isolated from cells by using RNeasy micro kit Qiagen (Qiagen, GmbH, Hilden, Germany). .. DNA was synthesized with Applied Biosystems High-Capacity cDNA Reverse Transcription Kit.

    Article Title: GEF-H1 Signaling upon Microtubule Destabilization Is Required for Dendritic Cell Activation and Specific Anti-tumor Responses
    Article Snippet: RNaseq and GSEA Analyses RNA was isolated from C57BL/6N WT and GEFH1−/− DCs using RNeasy Micro kit (QIAGEN) following the manufacturer’s instructions. .. Libraries were synthesized using Illumina TruSeq Stranded mRNA sample preparation kit from 500 ng of purified total RNA and indexed adaptors according to the manufacturer’s protocol (Illumina).

    Article Title: Variability of von Willebrand factor‐related parameters in endothelial colony forming cells. Variability of von Willebrand factor‐related parameters in endothelial colony forming cells
    Article Snippet: 2.7 Gene expression RNA was isolated using the RNeasy Micro Kit (Qiagen, Venlo, the Netherlands). .. Complementary DNA was synthesized using SuperScript™ II Reverse Transcriptase (Thermo Fisher Scientific) with poly(T) primers (Sigma‐Aldrich).

    Construct:

    Article Title: Myeloid lineage enhancers drive oncogene synergy in CEBPA/CSF3R mutant acute myeloid leukemia
    Article Snippet: .. RNA was extracted using the RNeasy micro kit (Qiagen). cDNA libraries were constructed using the SMARTer universal low input RNA kit (Clonetech) and sequenced using a HiSeq 2500 Sequencer (Illumina) 100 bp SR. .. Microarray Labeled target, sscDNA was prepared from 12 RNA samples using the GeneChipTM WT-Plus protocol (ThermoFisher).

    Real-time Polymerase Chain Reaction:

    Article Title: The role of receptor MAS in microglia-driven retinal vascular development
    Article Snippet: .. Quantitative PCR RNA was isolated from cells by using RNeasy micro kit Qiagen (Qiagen, GmbH, Hilden, Germany). .. DNA was synthesized with Applied Biosystems High-Capacity cDNA Reverse Transcription Kit.

    Article Title: Doxycycline attenuates cisplatin-induced acute kidney injury through pleiotropic effects
    Article Snippet: Paragraph title: Real-time quantitative polymerase chain reaction. ... Total RNA was extracted using TRIzol (Life Technologies, Grand Island, NY) and RNeasy Micro Kit (Qiagen, Valencia, CA).

    Article Title: Variability of von Willebrand factor‐related parameters in endothelial colony forming cells. Variability of von Willebrand factor‐related parameters in endothelial colony forming cells
    Article Snippet: 2.7 Gene expression RNA was isolated using the RNeasy Micro Kit (Qiagen, Venlo, the Netherlands). .. Quantitative PCR (qPCR) was performed using Sybr™ Select Master Mix (Thermo Fisher Scientific) in the CFX384 Touch instrument (Bio‐Rad, Veenendaal, the Netherlands) with GAPDH as endogenous reference gene.

    Article Title: Role of cell signaling in poxvirus-mediated foreign gene expression in mammalian cells
    Article Snippet: Paragraph title: DNA/RNA extraction, plasmid preparation, and real-time PCR ... Total RNA was isolated from virus-infected or uninfected cells by use of RNeasy Micro Kit (QIAGEN, Valencia, CA) following the manufacturer's protocol.

    Microarray:

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: RNA extraction was undertaken with a Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA), and quality was assessed using an Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, CA). .. The use of three rounds of amplification could induce degradation of RNA and potentially bias the microarray data; however, all the samples from both groups were processed in an identical fashion, making it unlikely that such bias occurred in one group to a greater degree than another.

    Incubation:

    Article Title: A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis *A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis * S⃞
    Article Snippet: Cells were then harvested in 500 μl of hypotonic buffer, incubated on ice for 30 min, and lysed by Dounce homogenization (40–50 strokes). .. Real-time Quantitative Reverse Transcription-PCR Analysis — The RNeasy® Micro kit (Qiagen) and SuperScript First Strand Synthesis kit (Invitrogen) were used for total RNA extraction and cDNA synthesis, respectively.

    Article Title: Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition
    Article Snippet: One volume of 70% EtOH was added to the lysate, transferred to an RNeasy MinElute spin column, and centrifuged for 15 s. Next, 350 μl of buffer RW1 from QIAGEN RNeasy Micro Kit was added to wash the RNeasy MinElute spin column by 15 s centrifugation. .. 10 μl DNase I mix was added to the RNeasy MinElute spin column membrane (10 μl DNase + 70 μl buffer RDD) and incubated at room temperature for 15 min.

    Article Title: Comparison of Candidate Cell Populations for the Recellularization of Decellularized Heart Valves
    Article Snippet: Fluorescent secondary antibodies (Alexa Fluor 488 or Alexa Fluor 594; Life Technologies) were then incubated for 1 h followed by nuclear counterstaining (DAPI; Life Technologies). .. RNA was isolated using the RNeasy Micro Kit (Qiagen) and then reverse transcribed to cDNA using the High Capacity cDNA Reverse Transcription Kit (Invitrogen). rt-PCR (7300 RT-PCR system, Applied Biosystems) was performed using a custom phenotyping array (TaqMan, Life Technologies).

    Activity Assay:

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: Each vial into which the laser-dissected specimens fell by gravity contained a small volume of a lysis/denaturing solution to inhibit RNAse activity. .. RNA extraction was undertaken with a Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA), and quality was assessed using an Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, CA).

    Infection:

    Article Title: Role of cell signaling in poxvirus-mediated foreign gene expression in mammalian cells
    Article Snippet: DNA was extracted from virus-bound or infected cells by use of DNeasy Tissue Kit (QIAGEN, Valencia, CA) according to the manufacturer's recommendation and extracted DNA samples were stored at -80°C until use. .. Total RNA was isolated from virus-infected or uninfected cells by use of RNeasy Micro Kit (QIAGEN, Valencia, CA) following the manufacturer's protocol.

    Expressing:

    Article Title: The role of receptor MAS in microglia-driven retinal vascular development
    Article Snippet: Quantitative PCR RNA was isolated from cells by using RNeasy micro kit Qiagen (Qiagen, GmbH, Hilden, Germany). .. The level of expression was normalized and expressed relatively to control using the ΔΔCt method.

    Article Title: Variability of von Willebrand factor‐related parameters in endothelial colony forming cells. Variability of von Willebrand factor‐related parameters in endothelial colony forming cells
    Article Snippet: .. 2.7 Gene expression RNA was isolated using the RNeasy Micro Kit (Qiagen, Venlo, the Netherlands). .. Complementary DNA was synthesized using SuperScript™ II Reverse Transcriptase (Thermo Fisher Scientific) with poly(T) primers (Sigma‐Aldrich).

    Article Title: Identification of cells initiating human melanomas
    Article Snippet: .. Real-time RT–PCR for BMPR1A, VE-cadherin, nestin and TIE1 gene expression analysis were performed as follows: total RNA was extracted from melanoma cells using the RNeasy Micro kit (Qiagen). .. Total RNA (5 μg) in 20 μl RT reaction mix was transcribed into complementary DNA using the SuperScript III First-Strand Synthesis System for RT–PCR (Invitrogen).

    Article Title: Comparison of Candidate Cell Populations for the Recellularization of Decellularized Heart Valves
    Article Snippet: Tissue samples from each leaflet of the valve were combined to form one sample to measure overall gene expression across the valve. .. RNA was isolated using the RNeasy Micro Kit (Qiagen) and then reverse transcribed to cDNA using the High Capacity cDNA Reverse Transcription Kit (Invitrogen). rt-PCR (7300 RT-PCR system, Applied Biosystems) was performed using a custom phenotyping array (TaqMan, Life Technologies).

    Western Blot:

    Article Title: A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis *A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis * S⃞
    Article Snippet: Proteins were separated by SDS-PAGE and analyzed by Western blot analysis. .. Real-time Quantitative Reverse Transcription-PCR Analysis — The RNeasy® Micro kit (Qiagen) and SuperScript First Strand Synthesis kit (Invitrogen) were used for total RNA extraction and cDNA synthesis, respectively.

    Immunohistochemistry:

    Article Title: Comparison of Candidate Cell Populations for the Recellularization of Decellularized Heart Valves
    Article Snippet: Paragraph title: Histology, IHC, and PCR ... RNA was isolated using the RNeasy Micro Kit (Qiagen) and then reverse transcribed to cDNA using the High Capacity cDNA Reverse Transcription Kit (Invitrogen). rt-PCR (7300 RT-PCR system, Applied Biosystems) was performed using a custom phenotyping array (TaqMan, Life Technologies).

    Dissection:

    Article Title: Comparison of Candidate Cell Populations for the Recellularization of Decellularized Heart Valves
    Article Snippet: Post-seeding tissue samples were isolated from each leaflet during valve dissection and immediately frozen in liquid nitrogen before being pulverized. .. RNA was isolated using the RNeasy Micro Kit (Qiagen) and then reverse transcribed to cDNA using the High Capacity cDNA Reverse Transcription Kit (Invitrogen). rt-PCR (7300 RT-PCR system, Applied Biosystems) was performed using a custom phenotyping array (TaqMan, Life Technologies).

    Cell Culture:

    Article Title: Myeloid lineage enhancers drive oncogene synergy in CEBPA/CSF3R mutant acute myeloid leukemia
    Article Snippet: For order of acquisition experiments, cells were cultured in cytokine-free I20 media for 48 h after sorting in the presence of 500 nM 4-Hydroxytamoxifen. .. RNA was extracted using the RNeasy micro kit (Qiagen). cDNA libraries were constructed using the SMARTer universal low input RNA kit (Clonetech) and sequenced using a HiSeq 2500 Sequencer (Illumina) 100 bp SR.

    Article Title: Effect of Proton Pump Inhibitor Therapy on NOX5, mPGES1 and iNOS expression in Barrett’s Esophagus
    Article Snippet: Reverse transcription-PCR Total RNA from esophageal biopsies was purified by using The RNeasy Micro Kit (Qiagen, Germantown, MD). .. TRIzol reagent (ThermoFisher Scientific, Foster City, CA) was used to purify total RNA from the cultured cells and GeneAmp Gold RNA PCR reagent kit (ThermoFisher Scientific, Foster City, CA) was utilized to reversely transcribe 1.5 μ g of total RNAs.

    Polymerase Chain Reaction:

    Article Title: Effect of Proton Pump Inhibitor Therapy on NOX5, mPGES1 and iNOS expression in Barrett’s Esophagus
    Article Snippet: Reverse transcription-PCR Total RNA from esophageal biopsies was purified by using The RNeasy Micro Kit (Qiagen, Germantown, MD). .. TRIzol reagent (ThermoFisher Scientific, Foster City, CA) was used to purify total RNA from the cultured cells and GeneAmp Gold RNA PCR reagent kit (ThermoFisher Scientific, Foster City, CA) was utilized to reversely transcribe 1.5 μ g of total RNAs.

    Article Title: EXPRESSION OF EGFL7 IN PRIMORDIAL GERM CELLS AND IN ADULT OVARIES AND TESTES
    Article Snippet: RNA from pre-implantation embryos was isolated using the RNeasy Micro Kit (Qiagen). .. PCR was performed using 2 µl of the reverse transcriptase reaction in a volume of 25 µl with Platinum Taq polymerase (Invitrogen).

    Article Title: Doxycycline attenuates cisplatin-induced acute kidney injury through pleiotropic effects
    Article Snippet: Total RNA was extracted using TRIzol (Life Technologies, Grand Island, NY) and RNeasy Micro Kit (Qiagen, Valencia, CA). .. Real-time polymerase chain reaction (PCR) was performed using an ABI PRISM 7900 Sequence Detector System (Applied Biosystems).

    Article Title: Identification of cells initiating human melanomas
    Article Snippet: Paragraph title: RNA extraction and real-time quantitative reverse transcription PCR (RT–PCR) ... Real-time RT–PCR for BMPR1A, VE-cadherin, nestin and TIE1 gene expression analysis were performed as follows: total RNA was extracted from melanoma cells using the RNeasy Micro kit (Qiagen).

    Article Title: Role of cell signaling in poxvirus-mediated foreign gene expression in mammalian cells
    Article Snippet: Total RNA was isolated from virus-infected or uninfected cells by use of RNeasy Micro Kit (QIAGEN, Valencia, CA) following the manufacturer's protocol. .. Both DNA and total RNA samples were quantified spectrophotopically prior to PCR amplification.

    Article Title: Comparison of Candidate Cell Populations for the Recellularization of Decellularized Heart Valves
    Article Snippet: Paragraph title: Histology, IHC, and PCR ... RNA was isolated using the RNeasy Micro Kit (Qiagen) and then reverse transcribed to cDNA using the High Capacity cDNA Reverse Transcription Kit (Invitrogen). rt-PCR (7300 RT-PCR system, Applied Biosystems) was performed using a custom phenotyping array (TaqMan, Life Technologies).

    Sequencing:

    Article Title: A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis *A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis * S⃞
    Article Snippet: Real-time Quantitative Reverse Transcription-PCR Analysis — The RNeasy® Micro kit (Qiagen) and SuperScript First Strand Synthesis kit (Invitrogen) were used for total RNA extraction and cDNA synthesis, respectively. .. Quantitative reverse transcription-PCR was carried out using the ABI 7900 Sequence Detection System, and results were analyzed using the comparative CT method of analysis and SDS 2.1 software (Applied Biosystems).

    Article Title: Human Cortical Organoids Expose a Differential Function of GSK3 on Cortical Neurogenesis
    Article Snippet: .. Total RNA Extraction and Sequencing Total RNA was isolated with the RNeasy Micro Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. .. RNA was quantified with Nanodrop and then the integrity was evaluated with Agilent 2100 Bioanalyzer.

    Article Title: GEF-H1 Signaling upon Microtubule Destabilization Is Required for Dendritic Cell Activation and Specific Anti-tumor Responses
    Article Snippet: RNaseq and GSEA Analyses RNA was isolated from C57BL/6N WT and GEFH1−/− DCs using RNeasy Micro kit (QIAGEN) following the manufacturer’s instructions. .. Pooled libraries were subjected to 35-bp paired-end sequencing according to the manufacturer’s protocol (Illumina Next-Seq 500).

    Article Title: Doxycycline attenuates cisplatin-induced acute kidney injury through pleiotropic effects
    Article Snippet: Total RNA was extracted using TRIzol (Life Technologies, Grand Island, NY) and RNeasy Micro Kit (Qiagen, Valencia, CA). .. Real-time polymerase chain reaction (PCR) was performed using an ABI PRISM 7900 Sequence Detector System (Applied Biosystems).

    Sonication:

    Article Title: A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis *A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis * S⃞
    Article Snippet: The insoluble pellet (nuclear plus membrane fraction) was resuspended in NTENT lysis buffer (10 m m Tris-HCl (pH 7.2), 150 m m NaCl, 1 m m EDTA, 1% Triton X-100 plus protease, and phosphatase inhibitors as listed above), sonicated on ice for ∼2–4 cycles, 10 s/cycle, and stored. .. Real-time Quantitative Reverse Transcription-PCR Analysis — The RNeasy® Micro kit (Qiagen) and SuperScript First Strand Synthesis kit (Invitrogen) were used for total RNA extraction and cDNA synthesis, respectively.

    RNA Sequencing Assay:

    Article Title: Myeloid lineage enhancers drive oncogene synergy in CEBPA/CSF3R mutant acute myeloid leukemia
    Article Snippet: Paragraph title: RNA sequencing ... RNA was extracted using the RNeasy micro kit (Qiagen). cDNA libraries were constructed using the SMARTer universal low input RNA kit (Clonetech) and sequenced using a HiSeq 2500 Sequencer (Illumina) 100 bp SR.

    Isolation:

    Article Title: The role of receptor MAS in microglia-driven retinal vascular development
    Article Snippet: .. Quantitative PCR RNA was isolated from cells by using RNeasy micro kit Qiagen (Qiagen, GmbH, Hilden, Germany). .. DNA was synthesized with Applied Biosystems High-Capacity cDNA Reverse Transcription Kit.

    Article Title: Human Cortical Organoids Expose a Differential Function of GSK3 on Cortical Neurogenesis
    Article Snippet: .. Total RNA Extraction and Sequencing Total RNA was isolated with the RNeasy Micro Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. .. RNA was quantified with Nanodrop and then the integrity was evaluated with Agilent 2100 Bioanalyzer.

    Article Title: EXPRESSION OF EGFL7 IN PRIMORDIAL GERM CELLS AND IN ADULT OVARIES AND TESTES
    Article Snippet: .. RNA from pre-implantation embryos was isolated using the RNeasy Micro Kit (Qiagen). .. Total RNA was reverse transcribed using oligo dT with the Superscript First Strand Synthesis System (Invitrogen) according to the manufacturer’s protocol.

    Article Title: GEF-H1 Signaling upon Microtubule Destabilization Is Required for Dendritic Cell Activation and Specific Anti-tumor Responses
    Article Snippet: .. RNaseq and GSEA Analyses RNA was isolated from C57BL/6N WT and GEFH1−/− DCs using RNeasy Micro kit (QIAGEN) following the manufacturer’s instructions. .. Libraries were synthesized using Illumina TruSeq Stranded mRNA sample preparation kit from 500 ng of purified total RNA and indexed adaptors according to the manufacturer’s protocol (Illumina).

    Article Title: Variability of von Willebrand factor‐related parameters in endothelial colony forming cells. Variability of von Willebrand factor‐related parameters in endothelial colony forming cells
    Article Snippet: .. 2.7 Gene expression RNA was isolated using the RNeasy Micro Kit (Qiagen, Venlo, the Netherlands). .. Complementary DNA was synthesized using SuperScript™ II Reverse Transcriptase (Thermo Fisher Scientific) with poly(T) primers (Sigma‐Aldrich).

    Article Title: Poised chromatin at the ZEB1 promoter enables cell plasticity and enhances tumorigenicity
    Article Snippet: .. Total RNA was isolated using the RNeasy Micro kit (Qiagen). .. Reverse transcription was performed with miScript II RT Kit, miScript and Qantitect Primer Assays were used to detect miRNAs and mRNA (Qiagen).

    Article Title: Role of cell signaling in poxvirus-mediated foreign gene expression in mammalian cells
    Article Snippet: .. Total RNA was isolated from virus-infected or uninfected cells by use of RNeasy Micro Kit (QIAGEN, Valencia, CA) following the manufacturer's protocol. .. Prior to RNA elution from an RNeasy column, DNA removal was performed by on-column DNase digestion for 15 min at room temperature using RNase-Free Set (QIAGEN, Valencia, CA).

    Article Title: Comparison of Candidate Cell Populations for the Recellularization of Decellularized Heart Valves
    Article Snippet: .. RNA was isolated using the RNeasy Micro Kit (Qiagen) and then reverse transcribed to cDNA using the High Capacity cDNA Reverse Transcription Kit (Invitrogen). rt-PCR (7300 RT-PCR system, Applied Biosystems) was performed using a custom phenotyping array (TaqMan, Life Technologies). .. PCR data is conveyed as the relative fold change in gene expression of the cells from tissue samples compared against the matched pre-seeding cell population.

    Labeling:

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: RNA extraction was undertaken with a Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA), and quality was assessed using an Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, CA). .. Subsequently, target labeling was performed with the Message-AMP Biotin Enhanced Kit (Ambion).

    Mouse Assay:

    Article Title: Myeloid lineage enhancers drive oncogene synergy in CEBPA/CSF3R mutant acute myeloid leukemia
    Article Snippet: RNA sequencing Bone marrow from C57BL/6 mice was harvested and retrovirally transduced with the indicated oncogene combinations as described above. .. RNA was extracted using the RNeasy micro kit (Qiagen). cDNA libraries were constructed using the SMARTer universal low input RNA kit (Clonetech) and sequenced using a HiSeq 2500 Sequencer (Illumina) 100 bp SR.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: EXPRESSION OF EGFL7 IN PRIMORDIAL GERM CELLS AND IN ADULT OVARIES AND TESTES
    Article Snippet: Paragraph title: 2.6. RT-PCR Analysis ... RNA from pre-implantation embryos was isolated using the RNeasy Micro Kit (Qiagen).

    Article Title: Identification of cells initiating human melanomas
    Article Snippet: Paragraph title: RNA extraction and real-time quantitative reverse transcription PCR (RT–PCR) ... Real-time RT–PCR for BMPR1A, VE-cadherin, nestin and TIE1 gene expression analysis were performed as follows: total RNA was extracted from melanoma cells using the RNeasy Micro kit (Qiagen).

    Article Title: Comparison of Candidate Cell Populations for the Recellularization of Decellularized Heart Valves
    Article Snippet: .. RNA was isolated using the RNeasy Micro Kit (Qiagen) and then reverse transcribed to cDNA using the High Capacity cDNA Reverse Transcription Kit (Invitrogen). rt-PCR (7300 RT-PCR system, Applied Biosystems) was performed using a custom phenotyping array (TaqMan, Life Technologies). .. PCR data is conveyed as the relative fold change in gene expression of the cells from tissue samples compared against the matched pre-seeding cell population.

    Quantitative RT-PCR:

    Article Title: GEF-H1 Signaling upon Microtubule Destabilization Is Required for Dendritic Cell Activation and Specific Anti-tumor Responses
    Article Snippet: RNaseq and GSEA Analyses RNA was isolated from C57BL/6N WT and GEFH1−/− DCs using RNeasy Micro kit (QIAGEN) following the manufacturer’s instructions. .. The final dsDNA libraries were quantified by Qubit fluorometer, Agilent Tapestation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols.

    Article Title: Identification of cells initiating human melanomas
    Article Snippet: .. Real-time RT–PCR for BMPR1A, VE-cadherin, nestin and TIE1 gene expression analysis were performed as follows: total RNA was extracted from melanoma cells using the RNeasy Micro kit (Qiagen). .. Total RNA (5 μg) in 20 μl RT reaction mix was transcribed into complementary DNA using the SuperScript III First-Strand Synthesis System for RT–PCR (Invitrogen).

    Article Title: Poised chromatin at the ZEB1 promoter enables cell plasticity and enhances tumorigenicity
    Article Snippet: Paragraph title: RNA preparation and qRT-PCR analysis ... Total RNA was isolated using the RNeasy Micro kit (Qiagen).

    Lysis:

    Article Title: A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis *A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis * S⃞
    Article Snippet: The insoluble pellet (nuclear plus membrane fraction) was resuspended in NTENT lysis buffer (10 m m Tris-HCl (pH 7.2), 150 m m NaCl, 1 m m EDTA, 1% Triton X-100 plus protease, and phosphatase inhibitors as listed above), sonicated on ice for ∼2–4 cycles, 10 s/cycle, and stored. .. Real-time Quantitative Reverse Transcription-PCR Analysis — The RNeasy® Micro kit (Qiagen) and SuperScript First Strand Synthesis kit (Invitrogen) were used for total RNA extraction and cDNA synthesis, respectively.

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: Each vial into which the laser-dissected specimens fell by gravity contained a small volume of a lysis/denaturing solution to inhibit RNAse activity. .. RNA extraction was undertaken with a Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA), and quality was assessed using an Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, CA).

    Purification:

    Article Title: Effect of Proton Pump Inhibitor Therapy on NOX5, mPGES1 and iNOS expression in Barrett’s Esophagus
    Article Snippet: .. Reverse transcription-PCR Total RNA from esophageal biopsies was purified by using The RNeasy Micro Kit (Qiagen, Germantown, MD). .. TRIzol reagent (ThermoFisher Scientific, Foster City, CA) was used to purify total RNA from the cultured cells and GeneAmp Gold RNA PCR reagent kit (ThermoFisher Scientific, Foster City, CA) was utilized to reversely transcribe 1.5 μ g of total RNAs.

    Article Title: GEF-H1 Signaling upon Microtubule Destabilization Is Required for Dendritic Cell Activation and Specific Anti-tumor Responses
    Article Snippet: RNaseq and GSEA Analyses RNA was isolated from C57BL/6N WT and GEFH1−/− DCs using RNeasy Micro kit (QIAGEN) following the manufacturer’s instructions. .. Libraries were synthesized using Illumina TruSeq Stranded mRNA sample preparation kit from 500 ng of purified total RNA and indexed adaptors according to the manufacturer’s protocol (Illumina).

    SDS Page:

    Article Title: A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis *A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis * S⃞
    Article Snippet: Proteins were separated by SDS-PAGE and analyzed by Western blot analysis. .. Real-time Quantitative Reverse Transcription-PCR Analysis — The RNeasy® Micro kit (Qiagen) and SuperScript First Strand Synthesis kit (Invitrogen) were used for total RNA extraction and cDNA synthesis, respectively.

    Plasmid Preparation:

    Article Title: Role of cell signaling in poxvirus-mediated foreign gene expression in mammalian cells
    Article Snippet: Paragraph title: DNA/RNA extraction, plasmid preparation, and real-time PCR ... Total RNA was isolated from virus-infected or uninfected cells by use of RNeasy Micro Kit (QIAGEN, Valencia, CA) following the manufacturer's protocol.

    Software:

    Article Title: A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis *A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis * S⃞
    Article Snippet: Real-time Quantitative Reverse Transcription-PCR Analysis — The RNeasy® Micro kit (Qiagen) and SuperScript First Strand Synthesis kit (Invitrogen) were used for total RNA extraction and cDNA synthesis, respectively. .. Quantitative reverse transcription-PCR was carried out using the ABI 7900 Sequence Detection System, and results were analyzed using the comparative CT method of analysis and SDS 2.1 software (Applied Biosystems).

    RNA Extraction:

    Article Title: A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis *A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis * S⃞
    Article Snippet: .. Real-time Quantitative Reverse Transcription-PCR Analysis — The RNeasy® Micro kit (Qiagen) and SuperScript First Strand Synthesis kit (Invitrogen) were used for total RNA extraction and cDNA synthesis, respectively. .. Quantitative reverse transcription-PCR was carried out using the ABI 7900 Sequence Detection System, and results were analyzed using the comparative CT method of analysis and SDS 2.1 software (Applied Biosystems).

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: .. RNA extraction was undertaken with a Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA), and quality was assessed using an Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, CA). .. Following the manufacturer's instructions, three rounds of linear amplification of the target was carried out using the MessageAmp aRNA Amplification kit (Ambion, Austin, TX).

    Article Title: Human Cortical Organoids Expose a Differential Function of GSK3 on Cortical Neurogenesis
    Article Snippet: .. Total RNA Extraction and Sequencing Total RNA was isolated with the RNeasy Micro Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. .. RNA was quantified with Nanodrop and then the integrity was evaluated with Agilent 2100 Bioanalyzer.

    Article Title: Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition
    Article Snippet: Paragraph title: RNA extraction with QIAGENRNeasy Micro Kit. ... One volume of 70% EtOH was added to the lysate, transferred to an RNeasy MinElute spin column, and centrifuged for 15 s. Next, 350 μl of buffer RW1 from QIAGEN RNeasy Micro Kit was added to wash the RNeasy MinElute spin column by 15 s centrifugation.

    Article Title: Identification of cells initiating human melanomas
    Article Snippet: Paragraph title: RNA extraction and real-time quantitative reverse transcription PCR (RT–PCR) ... Real-time RT–PCR for BMPR1A, VE-cadherin, nestin and TIE1 gene expression analysis were performed as follows: total RNA was extracted from melanoma cells using the RNeasy Micro kit (Qiagen).

    Positron Emission Tomography:

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: Briefly, frozen tissue blocks containing SNc from control subjects and patients with idiopathic Parkinson's disease matched for age and postmortem interval (PMI) were cut using a Microm HM 560 CryoStar cryostat (8 µm), mounted on LEICA Frame Slides with a PET-membrane (1.4 µm) and placed on a LEICA AS LMD apparatus. .. RNA extraction was undertaken with a Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA), and quality was assessed using an Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, CA).

    Sample Prep:

    Article Title: Human Cortical Organoids Expose a Differential Function of GSK3 on Cortical Neurogenesis
    Article Snippet: Total RNA Extraction and Sequencing Total RNA was isolated with the RNeasy Micro Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. .. A TruSeq Stranded Total RNA LT Sample Prep Kit (Illumina) was used for library preparation starting from 500 ng of total RNA for each sample.

    Article Title: GEF-H1 Signaling upon Microtubule Destabilization Is Required for Dendritic Cell Activation and Specific Anti-tumor Responses
    Article Snippet: RNaseq and GSEA Analyses RNA was isolated from C57BL/6N WT and GEFH1−/− DCs using RNeasy Micro kit (QIAGEN) following the manufacturer’s instructions. .. Libraries were synthesized using Illumina TruSeq Stranded mRNA sample preparation kit from 500 ng of purified total RNA and indexed adaptors according to the manufacturer’s protocol (Illumina).

    Homogenization:

    Article Title: A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis *A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-?-induced Apoptosis * S⃞
    Article Snippet: Cells were then harvested in 500 μl of hypotonic buffer, incubated on ice for 30 min, and lysed by Dounce homogenization (40–50 strokes). .. Real-time Quantitative Reverse Transcription-PCR Analysis — The RNeasy® Micro kit (Qiagen) and SuperScript First Strand Synthesis kit (Invitrogen) were used for total RNA extraction and cDNA synthesis, respectively.

    Article Title: Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition
    Article Snippet: Briefly, 25 embryos were disrupted by addition of buffer RLT followed by homogenization of the lysate. .. One volume of 70% EtOH was added to the lysate, transferred to an RNeasy MinElute spin column, and centrifuged for 15 s. Next, 350 μl of buffer RW1 from QIAGEN RNeasy Micro Kit was added to wash the RNeasy MinElute spin column by 15 s centrifugation.

    Laser Capture Microdissection:

    Article Title: Gene expression profiling of substantia nigra dopamine neurons: further insights into Parkinson's disease pathology
    Article Snippet: Since DA neurons contain neuromelanin, they could easily be visualized and collected using laser-based microdissection. .. RNA extraction was undertaken with a Qiagen RNeasy Micro Kit (Qiagen, Valencia, CA), and quality was assessed using an Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, CA).

    Standard Deviation:


    Staining:

    Article Title: Myeloid lineage enhancers drive oncogene synergy in CEBPA/CSF3R mutant acute myeloid leukemia
    Article Snippet: Cells were stained with a lineage cocktail, and lineage-negative GFP/mCherry-double-positive cells were sorted. .. RNA was extracted using the RNeasy micro kit (Qiagen). cDNA libraries were constructed using the SMARTer universal low input RNA kit (Clonetech) and sequenced using a HiSeq 2500 Sequencer (Illumina) 100 bp SR.

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    Qiagen rneasy micro kit
    Rneasy Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 2018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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