rneasy ffpe kit (Qiagen)
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Name:
RNeasy FFPE Kit
Description:
For purification of total RNA from formalin fixed paraffin embedded tissue sections Kit contents Qiagen RNeasy FFPE Kit 50 preps 14 to 30L Elution Volume FFPE Tissue Sample Total RNA Purification Spin Column Format Silica Technology Ideal for PCR qPCR Real time RT PCR Microarray Includes 50 RNeasy MinElute Spin Columns Collection Tubes Proteinase K RNase free DNase I DNase Booster Buffer RNase free Buffers RNase free Water Benefits Novel method to overcome formalin crosslinking Efficient release of RNA without compromising integrity Streamlined protocol providing RNA in just 85 minu
Catalog Number:
73504
Price:
445
Category:
RNeasy FFPE Kit
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Structured Review
Qiagen
rneasy ffpe kit
For purification of total RNA from formalin fixed paraffin embedded tissue sections Kit contents Qiagen RNeasy FFPE Kit 50 preps 14 to 30L Elution Volume FFPE Tissue Sample Total RNA Purification Spin Column Format Silica Technology Ideal for PCR qPCR Real time RT PCR Microarray Includes 50 RNeasy MinElute Spin Columns Collection Tubes Proteinase K RNase free DNase I DNase Booster Buffer RNase free Buffers RNase free Water Benefits Novel method to overcome formalin crosslinking Efficient release of RNA without compromising integrity Streamlined protocol providing RNA in just 85 minu
https://www.bioz.com/result/rneasy ffpe kit/product/Qiagen
Average 90 stars, based on 41175 article reviews
Price from $9.99 to $1999.99
For purification of total RNA from formalin fixed paraffin embedded tissue sections Kit contents Qiagen RNeasy FFPE Kit 50 preps 14 to 30L Elution Volume FFPE Tissue Sample Total RNA Purification Spin Column Format Silica Technology Ideal for PCR qPCR Real time RT PCR Microarray Includes 50 RNeasy MinElute Spin Columns Collection Tubes Proteinase K RNase free DNase I DNase Booster Buffer RNase free Buffers RNase free Water Benefits Novel method to overcome formalin crosslinking Efficient release of RNA without compromising integrity Streamlined protocol providing RNA in just 85 minu
https://www.bioz.com/result/rneasy ffpe kit/product/Qiagen
Average 90 stars, based on 41175 article reviews
Price from $9.99 to $1999.99
rneasy ffpe kit - by Bioz Stars,
2020-01
90/100 stars
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Images
1) Product Images from "Upregulation of P2Y2R, Active uPA, and PAI-1 Are Essential Components of Hantavirus Cardiopulmonary Syndrome"
Article Title: Upregulation of P2Y2R, Active uPA, and PAI-1 Are Essential Components of Hantavirus Cardiopulmonary Syndrome
Journal: Frontiers in Cellular and Infection Microbiology
doi: 10.3389/fcimb.2018.00169
Figure Legend Snippet: P2Y 2 R expression is significantly upregulated in HCPS compared to controls. RNA was extracted from lung tissues samples with the Qiagen RNeasy FFPE kit (cat# 73504) and quantified with a Thermo Fisher Scientific Nanodrop. P2Y 2 R expression levels were measured by the TaqMan assay using a WT P2Y 2 R plasmid as standard. P2Y 2 R copy #s were normalized to total RNA extracted from the embedded tissues. Each sample slice was measured in triplicate. (A) The plot of P2Y 2 R for each HCPS patient, shown on the x-axis as H1-H22. Each data point per patient represents a triplicate measurement of P2Y 2 R expression in a 10 μm slice cut from the FFPE blocks. The number of segments analyzed is shown for each case above the replicate data points (e.g., n = 11 for H1). Ninety-seven distinct segments from all cases were analyzed. Lung tissues from two decedents (H4, and H9) were not available. The error bars represent the median and range. For all HCPS samples, the minimum, median, maximum and mean values were 0.0055, 2.10, 24.88, and 4.1 ± 0.48 (SEM). (B,C) Plot of P2Y 2 R for pneumonia (P) and gunshot wound cases (GSW). The experimental conditions are similar to those described for HCPS. Cases for which no lung tissue samples were available were excluded from the graph. For all pneumonia tissue segment samples ( n = 39), the minimum, median, maximum and mean values were 0.0005, 0.07, 4.4, 0.54 ± 0.16. For all GSW samples ( n = 21), the minimum, median, maximum and mean values were 0.0052, 0.18, 2.4, and 0.46 ± 0.10 (SEM).
Techniques Used: Expressing, Formalin-fixed Paraffin-Embedded, TaqMan Assay, Plasmid Preparation
2) Product Images from "Upregulation of P2Y2R, Active uPA, and PAI-1 Are Essential Components of Hantavirus Cardiopulmonary Syndrome"
Article Title: Upregulation of P2Y2R, Active uPA, and PAI-1 Are Essential Components of Hantavirus Cardiopulmonary Syndrome
Journal: Frontiers in Cellular and Infection Microbiology
doi: 10.3389/fcimb.2018.00169
Figure Legend Snippet: P2Y 2 R expression is significantly upregulated in HCPS compared to controls. RNA was extracted from lung tissues samples with the Qiagen RNeasy FFPE kit (cat# 73504) and quantified with a Thermo Fisher Scientific Nanodrop. P2Y 2 R expression levels were measured by the TaqMan assay using a WT P2Y 2 R plasmid as standard. P2Y 2 R copy #s were normalized to total RNA extracted from the embedded tissues. Each sample slice was measured in triplicate. (A) The plot of P2Y 2 R for each HCPS patient, shown on the x-axis as H1-H22. Each data point per patient represents a triplicate measurement of P2Y 2 R expression in a 10 μm slice cut from the FFPE blocks. The number of segments analyzed is shown for each case above the replicate data points (e.g., n = 11 for H1). Ninety-seven distinct segments from all cases were analyzed. Lung tissues from two decedents (H4, and H9) were not available. The error bars represent the median and range. For all HCPS samples, the minimum, median, maximum and mean values were 0.0055, 2.10, 24.88, and 4.1 ± 0.48 (SEM). (B,C) Plot of P2Y 2 R for pneumonia (P) and gunshot wound cases (GSW). The experimental conditions are similar to those described for HCPS. Cases for which no lung tissue samples were available were excluded from the graph. For all pneumonia tissue segment samples ( n = 39), the minimum, median, maximum and mean values were 0.0005, 0.07, 4.4, 0.54 ± 0.16. For all GSW samples ( n = 21), the minimum, median, maximum and mean values were 0.0052, 0.18, 2.4, and 0.46 ± 0.10 (SEM).
Techniques Used: Expressing, Formalin-fixed Paraffin-Embedded, TaqMan Assay, Plasmid Preparation
3) Product Images from "Reduced expression levels of the death-associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma"
Article Title: Reduced expression levels of the death-associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma
Journal: Experimental and Therapeutic Medicine
doi: 10.3892/etm.2013.916
Figure Legend Snippet: Quantitative RT-PCR analysis of DAPK and E-cadherin mRNA levels in ESCC and adjacent normal tissues. Total RNA was harvested from tissues using the RNeasy FFPE kit (Qiagen) according to the manufacturer’s instructions. The RT-PCR experiments were repeated at least 3 times. RNA was reverse transcribed into cDNA using random primers in a Reverse Transcription II system (Promega) according to the manufacturer’s instructions. Expression of DAPK and E-cadherin mRNAs was quantified by quantitative PCR using an ABI Prism Sequence Detection System (Applied Biosystems). Template-negative and RT-negative conditions were used as controls. Amplification of the endogenous GAPDH cDNA was monitored. Levels (mean values) of DAPK and E-cadherin transcripts in patients were calculated. RT-PCR, reverse transcription-PCR; ESCC, esophageal squamous cell carcinoma; DAPK, death-associated protein kinase.
Techniques Used: Quantitative RT-PCR, Formalin-fixed Paraffin-Embedded, Reverse Transcription Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction, Sequencing, Amplification, Polymerase Chain Reaction
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Ovarian gene expression analysis by qPCR Purification of total RNA from formalin fixed, paraffin-embedded microdissected ovarian tissue was made by Reverse Transcription Polymerase Chain Reaction:Article Title: Ribonucleic Acid Extraction From Archival Formalin Fixed Paraffin Embedded Myocardial Tissues for Gene Expression and Pathogen Detection Article Snippet: Paragraph title: Expression of GAPDH by RT ‐ PCR ... mRNA of GAPDH was detected in RNA of all the 16 samples (100%) extracted by Article Title: EZH2 Is Associated with Malignant Behavior in Pancreatic IPMN via p27Kip1 Downregulation Article Snippet: Then, total RNA was extracted using Quantitative RT-PCR:Article Title: CD164 identifies CD4+ T cells highly expressing genes associated with malignancy in Sézary syndrome: the Sézary signature genes, FCRL3, Tox and miR-214 Article Snippet: For skin samples, total RNA was extracted from formalin-fixed paraffin embedded (FFPE) samples and normal tissue using the Qiagen Article Title: Targeted Resequencing Reveals ALK Fusions in Non-Small Cell Lung Carcinomas Detected by FISH, Immunohistochemistry, and Real-Time RT-PCR: A Comparison of Four Methods Article Snippet: Paragraph title: 2.4. Real-Time RT-PCR ... Following xylene deparaffinization of a 16 μ M FFPE section, total RNA was extracted using the Article Title: LMO2 Is a Novel Predictive Marker for a Better Prognosis in Pancreatic Cancer 1 Article Snippet: After the microdissection, total RNA was extracted from the selected cells and subjected to qRT-PCR for quantification of LMO2 as described previously [ ]. .. Total RNA was extracted using an SYBR Green Assay:Article Title: Phase II trial of dacomitinib, a pan–human EGFR tyrosine kinase inhibitor, in recurrent glioblastoma patients with EGFR amplification Article Snippet: Total RNA from FFPE tissues of EGFR amplified tumors was extracted using the Article Title: Premature ovarian aging in BRCA carriers: a prototype of systemic precocious aging? Article Snippet: Ovarian gene expression analysis by qPCR Purification of total RNA from formalin fixed, paraffin-embedded microdissected ovarian tissue was made by RNA Extraction:Article Title: Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers Article Snippet: Paragraph title: 4.3. RNA Extraction from Paraffin-Embedded Material ... For total RNA isolation, Article Title: LMO2 Is a Novel Predictive Marker for a Better Prognosis in Pancreatic Cancer 1 Article Snippet: Macrodissection was performed using a safety blade to enrich the neoplastic cell population, and 3 to 10 sections were used for RNA extraction. .. Total RNA was extracted using an Article Title: The lincRNA MIRAT binds to IQGAP1 and modulates the MAPK pathway in NRAS mutant melanoma Article Snippet: Paragraph title: Patient sample collection and RNA extraction ... RNA was extracted using Article Title: EZH2 Is Associated with Malignant Behavior in Pancreatic IPMN via p27Kip1 Downregulation Article Snippet: Paragraph title: RNA extraction from formalin-fixed paraffin-embedded specimens and real time PCR analyses ... Then, total RNA was extracted using Laser Capture Microdissection:Article Title: LMO2 Is a Novel Predictive Marker for a Better Prognosis in Pancreatic Cancer 1 Article Snippet: After the microdissection, total RNA was extracted from the selected cells and subjected to qRT-PCR for quantification of LMO2 as described previously [ ]. .. Total RNA was extracted using an Quantitation Assay:Article Title: Targeted Resequencing Reveals ALK Fusions in Non-Small Cell Lung Carcinomas Detected by FISH, Immunohistochemistry, and Real-Time RT-PCR: A Comparison of Four Methods Article Snippet: Following xylene deparaffinization of a 16 μ M FFPE section, total RNA was extracted using the Spectrophotometry:Article Title: Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers Article Snippet: For total RNA isolation, Staining:Article Title: EZH2 Is Associated with Malignant Behavior in Pancreatic IPMN via p27Kip1 Downregulation Article Snippet: IPMN lesions and normal pancreatic ducts were macro-dissectioned in comparison with H & E staining ( ). .. Then, total RNA was extracted using |
