7300 real time pcr system  (Thermo Fisher)


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    Name:
    7300 Real Time PCR Systems Spectral Calibration Kit
    Description:
    This easy to use kit establishes the pure dye spectra and multicomponent values for FAM SYBR Green I VIC JOE and NED TAMRA ROX dyes on the Applied Biosystems 7300 Real Time PCR System The Applied Biosystems 7300 Real Time PCR Systems Spectral Calibration Kit I contains one background plate one region of interest ROI calibration plate and seven spectral calibration plates with seven separate dye standards • 96 well optical reaction plates preloaded with dyes FAM SYBR Green I VIC JOE NED TAMRA ROX enable you to calibrate the 7300 Real Time PCR System quickly and easily• Easy to use preloaded spectral calibration plates reduce setup time• Provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture• Includes the Passive Reference I dye standard for signal normalization in all real time and endpoint reactionsSpend Your Time on Research Not Instrument CalibrationThis kit is easy to use preloaded 96 well optical reaction plates enable quick and easy calibration of the 7300 Real Time PCR System Insert the reaction plates during system installation and the system automatically collects the dye standard s spectral information The system s application algorithm then uses this calibration information to ensure accurate data analysis
    Catalog Number:
    4349182
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Real Time PCR (qPCR)|Real-Time PCR Instruments, Software & Calibration
    Category:
    Standards Ladders Controls
    Buy from Supplier


    Structured Review

    Thermo Fisher 7300 real time pcr system
    Real-time quantitative <t>RT-PCR</t> analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems <t>7300</t> Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    This easy to use kit establishes the pure dye spectra and multicomponent values for FAM SYBR Green I VIC JOE and NED TAMRA ROX dyes on the Applied Biosystems 7300 Real Time PCR System The Applied Biosystems 7300 Real Time PCR Systems Spectral Calibration Kit I contains one background plate one region of interest ROI calibration plate and seven spectral calibration plates with seven separate dye standards • 96 well optical reaction plates preloaded with dyes FAM SYBR Green I VIC JOE NED TAMRA ROX enable you to calibrate the 7300 Real Time PCR System quickly and easily• Easy to use preloaded spectral calibration plates reduce setup time• Provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture• Includes the Passive Reference I dye standard for signal normalization in all real time and endpoint reactionsSpend Your Time on Research Not Instrument CalibrationThis kit is easy to use preloaded 96 well optical reaction plates enable quick and easy calibration of the 7300 Real Time PCR System Insert the reaction plates during system installation and the system automatically collects the dye standard s spectral information The system s application algorithm then uses this calibration information to ensure accurate data analysis
    https://www.bioz.com/result/7300 real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 5271 article reviews
    Price from $9.99 to $1999.99
    7300 real time pcr system - by Bioz Stars, 2021-01
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    Images

    1) Product Images from "DIFFERENCES IN CYSTEINE PROTEASE ACTIVITY IN SCHISTOSOMA MANSONI-RESISTANT AND -SUSCEPTIBLE BIOMPHALARIA GLABRATA AND CHARACTERIZATION OF THE HEPATOPANCREAS CATHEPSIN B FULL-LENGTH cDNA"

    Article Title: DIFFERENCES IN CYSTEINE PROTEASE ACTIVITY IN SCHISTOSOMA MANSONI-RESISTANT AND -SUSCEPTIBLE BIOMPHALARIA GLABRATA AND CHARACTERIZATION OF THE HEPATOPANCREAS CATHEPSIN B FULL-LENGTH cDNA

    Journal: The Journal of parasitology

    doi: 10.1645/GE-1410R.1

    Real-time quantitative RT-PCR analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems 7300 Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).
    Figure Legend Snippet: Real-time quantitative RT-PCR analysis of the snail hepatopancreas cathepsin B transcript in resistant (BS-90) and susceptible (NMRI and M-line) pre- and postexposure to miracidia. RT-PCR was performed with the cathepsin B gene-specific primers using RNA from individual snails after various times postexposure. Gene-specific primers for the housekeeping gene myoglobin were used to assess sample uniformity and to confirm that the template cDNA was used in equivalent amounts for each amplification reaction. RT-PCR reactions were performed using an Applied Biosystems 7300 Real-Time PCR System, and detection of the fluorescently labeled product was performed at the end of the amplification period. The -fold increase of gene expression was calculated by comparative Ct method as described in Materials and Methods. A value of > 1-fold increase was considered to be significant (1 = value for nonexposed snails).

    Techniques Used: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Labeling, Expressing

    2) Product Images from "LACE1 interacts with p53 and mediates its mitochondrial translocation and apoptosis"

    Article Title: LACE1 interacts with p53 and mediates its mitochondrial translocation and apoptosis

    Journal: Oncotarget

    doi: 10.18632/oncotarget.9959

    Loss of LACE1 leads to increased apoptotic resistance whereas its overexpression results in increased apoptotic sensitivity A. Overexpression of LACE1-FLAG in both wild-type HEK293 cells and LACE1 KD cells results in increased PARP cleavage. Wild-type HEK293 cells and LACE1 KD cells were transfected with LACE1-FLAG expression vector or with the empty vector. Whole-cell lysates were analyzed with western blotting using antibody to cleaved PARP. Antibody to α -tubulin was used to monitor equal protein loading. B. LACE1 KD cells exhibit increased resistance to staurosporine-induced apoptosis but respond normally to TNFalpha-treatment. Control and LACE1 KD cells were treated with staurosporine (STS; 2 μM) or TNFalpha (10 ng/ml) and IFN-gamma (80 ng/ml) for 0, 3 and 6 hours. Whole cell lysates were prepared from treated cells and used for immunoblotting with antibody to cleaved PARP. Antibody to α-tubulin was used to control for equal protein loading. C. Cells were treated with staurosporine (STS; 2 μM) for 0, 3 and 6 hours and then harvested and used for subcellular fractionation using dounce homogenization and differential centrifugation. The resulting cytoplasmic fractions were subjected to SDS-PAGE western blotting with antibody to cytochrome c. Western blotting of alpha-tubulin was used as loading control. D. Cells grown on coverslips were treated with staurosporine (STS; 2 μM) for 0, 3 and 6 hours and then fixed with 4% paraformaldehyde at room temperature and permeabilized with 0.1% Triton X-100. Cells were blocked by 10% Fetal Bovine Serum and primary detection was performed with anti-cytochrome c antibody. After secondary fluorescent detection, cells were analyzed at 24°C using a Nikon Diaphot 200 inverted microscope equipped with a Plan-Apochromat 60×, numerical aperture 0.95, oil objective. The images were acquired with an Olympus DP50 CCD camera and Viewfinder Lite 1.0 software. Bar, 10 μM. E. Whereas knockdown of LACE1 leads to moderate upregulation of PUMA and BAX mRNAs, overexpression of LACE1-FLAG results in their significant downregulation. Relative mRNA quantification was performed using TaqMan Gene Expression Assays according to the manufacturer's instructions (Applied Biosystems). Data were collected in duplicate in two separate runs using a 7300 Real-Time PCR System (Applied Biosystems). HPRT1 (hypoxanthine phosphoribosyltransferase 1), TUBA1A (tubulin, alpha 1a) and TBP (TATA box–binding protein) were used as reference genes.
    Figure Legend Snippet: Loss of LACE1 leads to increased apoptotic resistance whereas its overexpression results in increased apoptotic sensitivity A. Overexpression of LACE1-FLAG in both wild-type HEK293 cells and LACE1 KD cells results in increased PARP cleavage. Wild-type HEK293 cells and LACE1 KD cells were transfected with LACE1-FLAG expression vector or with the empty vector. Whole-cell lysates were analyzed with western blotting using antibody to cleaved PARP. Antibody to α -tubulin was used to monitor equal protein loading. B. LACE1 KD cells exhibit increased resistance to staurosporine-induced apoptosis but respond normally to TNFalpha-treatment. Control and LACE1 KD cells were treated with staurosporine (STS; 2 μM) or TNFalpha (10 ng/ml) and IFN-gamma (80 ng/ml) for 0, 3 and 6 hours. Whole cell lysates were prepared from treated cells and used for immunoblotting with antibody to cleaved PARP. Antibody to α-tubulin was used to control for equal protein loading. C. Cells were treated with staurosporine (STS; 2 μM) for 0, 3 and 6 hours and then harvested and used for subcellular fractionation using dounce homogenization and differential centrifugation. The resulting cytoplasmic fractions were subjected to SDS-PAGE western blotting with antibody to cytochrome c. Western blotting of alpha-tubulin was used as loading control. D. Cells grown on coverslips were treated with staurosporine (STS; 2 μM) for 0, 3 and 6 hours and then fixed with 4% paraformaldehyde at room temperature and permeabilized with 0.1% Triton X-100. Cells were blocked by 10% Fetal Bovine Serum and primary detection was performed with anti-cytochrome c antibody. After secondary fluorescent detection, cells were analyzed at 24°C using a Nikon Diaphot 200 inverted microscope equipped with a Plan-Apochromat 60×, numerical aperture 0.95, oil objective. The images were acquired with an Olympus DP50 CCD camera and Viewfinder Lite 1.0 software. Bar, 10 μM. E. Whereas knockdown of LACE1 leads to moderate upregulation of PUMA and BAX mRNAs, overexpression of LACE1-FLAG results in their significant downregulation. Relative mRNA quantification was performed using TaqMan Gene Expression Assays according to the manufacturer's instructions (Applied Biosystems). Data were collected in duplicate in two separate runs using a 7300 Real-Time PCR System (Applied Biosystems). HPRT1 (hypoxanthine phosphoribosyltransferase 1), TUBA1A (tubulin, alpha 1a) and TBP (TATA box–binding protein) were used as reference genes.

    Techniques Used: Over Expression, Transfection, Expressing, Plasmid Preparation, Western Blot, Fractionation, Homogenization, Centrifugation, SDS Page, Inverted Microscopy, Software, Real-time Polymerase Chain Reaction, Binding Assay

    3) Product Images from "Knockdown of CypA inhibits interleukin-8 (IL-8) and IL-8-mediated proliferation and tumor growth of glioblastoma cells through down-regulated NF-?B"

    Article Title: Knockdown of CypA inhibits interleukin-8 (IL-8) and IL-8-mediated proliferation and tumor growth of glioblastoma cells through down-regulated NF-?B

    Journal: Journal of Neuro-Oncology

    doi: 10.1007/s11060-010-0220-y

    CypA KD-mediated down-regulation of IL-8 expression. a IL - 8 expression levels were determined by a quantitative real-time PCR analysis. One microgram of total RNA was used for cDNA synthesis, using Superscript III (Invitrogen) with the oligo(dT) 15 primer. Quantitative real-time PCR (QRT-PCR) was performed using the Power SYBR Green PCR Master Mix (Applied Biosystem) detected by the 7300 Real Time PCR System (Applied Biosystems). The relative expression of IL - 8 mRNA normalized to the internal reference 18S rRNA was analyzed using the 2 −ΔΔCT method ( columns , mean of results from three independent experiments; bars, SE; *, P
    Figure Legend Snippet: CypA KD-mediated down-regulation of IL-8 expression. a IL - 8 expression levels were determined by a quantitative real-time PCR analysis. One microgram of total RNA was used for cDNA synthesis, using Superscript III (Invitrogen) with the oligo(dT) 15 primer. Quantitative real-time PCR (QRT-PCR) was performed using the Power SYBR Green PCR Master Mix (Applied Biosystem) detected by the 7300 Real Time PCR System (Applied Biosystems). The relative expression of IL - 8 mRNA normalized to the internal reference 18S rRNA was analyzed using the 2 −ΔΔCT method ( columns , mean of results from three independent experiments; bars, SE; *, P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, SYBR Green Assay, Polymerase Chain Reaction

    4) Product Images from "Gastric peroxisome proliferator activator receptor-? expression and cytoprotective actions of its ligands against ischemia-reperfusion injury in rats"

    Article Title: Gastric peroxisome proliferator activator receptor-? expression and cytoprotective actions of its ligands against ischemia-reperfusion injury in rats

    Journal: Journal of Clinical Biochemistry and Nutrition

    doi: 10.3164/jcbn.10-81

    Quantitative real-time PCR for genes up-regulated by pioglitazone treatment. cDNA was prepared from gastric mucosa, and PCR was performed using an ABI 7300. The bars show levels of expression of each gene normalized to that of β-actin.
    Figure Legend Snippet: Quantitative real-time PCR for genes up-regulated by pioglitazone treatment. cDNA was prepared from gastric mucosa, and PCR was performed using an ABI 7300. The bars show levels of expression of each gene normalized to that of β-actin.

    Techniques Used: Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Expressing

    5) Product Images from "Alkylation of template strand of coding region causes effective gene silencing"

    Article Title: Alkylation of template strand of coding region causes effective gene silencing

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkl005

    Results of real-time quantification of PCR GFP ( a ) and β-actin ( b ) mRNAs in HCT116 cells treated with polyamides A–C. To evaluate the amount of transcribed GFP mRNA, TaqMan real-time PCR was performed with the 7300 Real-Time PCR System. The amount of GFP and β-actin mRNAs in the HCT116 cells were measured using pairs of primers with the TaqMan probes for each gene. The amount of mRNAs in HCT116 cells treated with 100 nM polyamides for 24 h was calculated with reference to the cells treated with 0.1% DMF (Control), set as 100%. Compared with the control, HCT116 cells treated with polyamide C showed significantly decreased GFP mRNA expression. The cells which the GFP vectors were not transfected (Mock) did not express GFP mRNA. Error bars, standard deviation of the means of triplicate samples. * P
    Figure Legend Snippet: Results of real-time quantification of PCR GFP ( a ) and β-actin ( b ) mRNAs in HCT116 cells treated with polyamides A–C. To evaluate the amount of transcribed GFP mRNA, TaqMan real-time PCR was performed with the 7300 Real-Time PCR System. The amount of GFP and β-actin mRNAs in the HCT116 cells were measured using pairs of primers with the TaqMan probes for each gene. The amount of mRNAs in HCT116 cells treated with 100 nM polyamides for 24 h was calculated with reference to the cells treated with 0.1% DMF (Control), set as 100%. Compared with the control, HCT116 cells treated with polyamide C showed significantly decreased GFP mRNA expression. The cells which the GFP vectors were not transfected (Mock) did not express GFP mRNA. Error bars, standard deviation of the means of triplicate samples. * P

    Techniques Used: Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Expressing, Transfection, Standard Deviation

    6) Product Images from "Novel Marine Phenazines as Potential Cancer Chemopreventive and Anti-Inflammatory Agents"

    Article Title: Novel Marine Phenazines as Potential Cancer Chemopreventive and Anti-Inflammatory Agents

    Journal: Marine Drugs

    doi: 10.3390/md10020451

    Effect of phenazines on COX-2 (black) and iNOS (gray) mRNA expression in RAW 264.7 cells. Total RNA was isolated using the TRIZOL ® Reagent method (Invitrogen) from RAW 264.7 cells (2 × 10 5 cells/well) after treatment with samples. cDNA was synthesized using the RT 2 First Strand Kit (C-03, SA Biosciences) protocol. cDNA was used for quantitative real time PCRs with fluorescent Power SyBR ® Green PCR master mix (Applied Biosystems), employing GAPDH , iNOS , COX-2 specific primers, and a 7300 Real Time PCR System (Applied Biosystems). The results were derived from two independent RNA preparations employing identical triplicates in each analysis and quantitated using GAPDH as the internal control, following the manufacturer’s instructions. ( A ) GAPDH standard curve for quantitation of iNOS and COX-2 expression; ( B ) Levels of COX-2 (black) and iNOS (gray) mRNA expression. The concentration and duration of treatment had no significant effect on the viability of Raw 264.7 cells.
    Figure Legend Snippet: Effect of phenazines on COX-2 (black) and iNOS (gray) mRNA expression in RAW 264.7 cells. Total RNA was isolated using the TRIZOL ® Reagent method (Invitrogen) from RAW 264.7 cells (2 × 10 5 cells/well) after treatment with samples. cDNA was synthesized using the RT 2 First Strand Kit (C-03, SA Biosciences) protocol. cDNA was used for quantitative real time PCRs with fluorescent Power SyBR ® Green PCR master mix (Applied Biosystems), employing GAPDH , iNOS , COX-2 specific primers, and a 7300 Real Time PCR System (Applied Biosystems). The results were derived from two independent RNA preparations employing identical triplicates in each analysis and quantitated using GAPDH as the internal control, following the manufacturer’s instructions. ( A ) GAPDH standard curve for quantitation of iNOS and COX-2 expression; ( B ) Levels of COX-2 (black) and iNOS (gray) mRNA expression. The concentration and duration of treatment had no significant effect on the viability of Raw 264.7 cells.

    Techniques Used: Expressing, Isolation, Synthesized, SYBR Green Assay, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Derivative Assay, Quantitation Assay, Concentration Assay

    7) Product Images from "Transformations of the macromolecular landscape at mitochondria during DNA-damage-induced apoptotic cell death"

    Article Title: Transformations of the macromolecular landscape at mitochondria during DNA-damage-induced apoptotic cell death

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2014.405

    Dox enhances the levels of mtDNA. ( a – d ) ATPase 8 and cytochrome c oxidase subunit II (COX II) genes encoded by the mitochondrial genome was amplified and quantified by real-time PCR using the SYBR green chemistry on the Applied Biosystems 7300 real-time PCR system. Total DNA was extracted from HCT116 WT and HCT116 Bax −/− cells treated with DMSO or Dox (10 μ M) for indicated times. COX II ( a and b ) or mitochondrial ATPase 8 ( c and d ) gene-specific primers were used to amplify, and values were normalized to actin or GAPDH. Data are mean±S.D., n =3; * P
    Figure Legend Snippet: Dox enhances the levels of mtDNA. ( a – d ) ATPase 8 and cytochrome c oxidase subunit II (COX II) genes encoded by the mitochondrial genome was amplified and quantified by real-time PCR using the SYBR green chemistry on the Applied Biosystems 7300 real-time PCR system. Total DNA was extracted from HCT116 WT and HCT116 Bax −/− cells treated with DMSO or Dox (10 μ M) for indicated times. COX II ( a and b ) or mitochondrial ATPase 8 ( c and d ) gene-specific primers were used to amplify, and values were normalized to actin or GAPDH. Data are mean±S.D., n =3; * P

    Techniques Used: Amplification, Real-time Polymerase Chain Reaction, SYBR Green Assay

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Knockdown of CypA inhibits interleukin-8 (IL-8) and IL-8-mediated proliferation and tumor growth of glioblastoma cells through down-regulated NF-?B
    Article Snippet: .. Quantitative real-time PCR (QRT-PCR) was performed using the Power SYBR Green PCR Master Mix (Applied Biosystem) and detected by the 7300 Real Time PCR System (Applied Biosystem). .. The relative expression of IL -8 mRNA normalized to the internal reference 18S rRNA was analyzed using the 2T −ΔΔC method [ ].

    Article Title: Ralstonia solanacearum promotes pathogenicity by utilizing l‐glutamic acid from host plants, et al. Ralstonia solanacearum promotes pathogenicity by utilizing l‐glutamic acid from host plants
    Article Snippet: .. HTSeq v. 0.6.1 was used to count the read numbers mapped to each gene, and the fragments per kilobase of transcript per million reads mapped (FPKM) of each gene was calculated based on the length of the gene and read counts mapped to the gene (Trapnell et al., ). cDNA synthesis and RT‐qPCR analysis were performed with the ChamQ Universal SYBR qPCR Master Mix (Vazyme) on a 7300 Plus real‐time PCR system (Applied Biosystems) following the manufacturer's instructions. .. The gene expression level of recA was used as a control for RT‐qPCR analysis.

    Article Title: Antimelanogenic effect of c-phycocyanin through modulation of tyrosinase expression by upregulation of ERK and downregulation of p38 MAPK signaling pathways
    Article Snippet: .. Quantitative PCR Quantitative PCR (Q-PCR) was performed with reaction mixtures containing total RNA (100 ng), one-step RT-PCR Master Mix Reagents (Applied Biosystems, Foster City, CA, USA), and probes (MITF, GAPDH ) on 7300 Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). .. Reverse transcription-polymerase chain reaction (RT-PCR) RT-PCR was performed by a two-step procedure, reverse transcription and PCR.

    Article Title: Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration
    Article Snippet: .. To analyze the expression of specific genes for osteoblastic differentiation, the expression of Runx2, osterix, ALP, collagen I, and osteopontin (OPN) was determined quantitatively on a real-time PCR machine (ABI 7300; Applied Biosystems, Foster City, CA, ) using a SYBR Premix Ex Taq kit (Takara), with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the reference gene for normalization. .. The expression of E-cadherin, vimentin, Klf4, Snai1, transforming growth factor-β (TGF-β) receptor type 1, and TGF-β1, key genes involved in epithelial-mesenchymal transformation (EMT) of hAECs [ ], were also determined quantitatively.

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury
    Article Snippet: .. Real-time qRT-PCR assay was used to quantitate the mRNA abundance with 7300 real-time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: Divergent Roles for Airway Epithelial MMP7 and Retinoic Acid in Experimental Asthma
    Article Snippet: .. One step real-time quantitative RT-PCR was used to determine the relative expression of Ccl11 (eotaxin-1) and Raldh-1 in Real-Time PCR System (7300 Applied Biosystems). .. The primer and probe mix of target genes, (Ccl11 : Mm00441238_m1, Raldh-1 : Mm01194995_mH) and 18S rRNA were purchased from Applied Biosystems.

    Article Title: DIFFERENCES IN CYSTEINE PROTEASE ACTIVITY IN SCHISTOSOMA MANSONI-RESISTANT AND -SUSCEPTIBLE BIOMPHALARIA GLABRATA AND CHARACTERIZATION OF THE HEPATOPANCREAS CATHEPSIN B FULL-LENGTH cDNA
    Article Snippet: .. The RT-PCR reactions were performed using an Applied Biosystems 7300 Real-Time PCR System (Applied Biosystems, Foster City, California). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Antimelanogenic effect of c-phycocyanin through modulation of tyrosinase expression by upregulation of ERK and downregulation of p38 MAPK signaling pathways
    Article Snippet: .. Quantitative PCR Quantitative PCR (Q-PCR) was performed with reaction mixtures containing total RNA (100 ng), one-step RT-PCR Master Mix Reagents (Applied Biosystems, Foster City, CA, USA), and probes (MITF, GAPDH ) on 7300 Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). .. Reverse transcription-polymerase chain reaction (RT-PCR) RT-PCR was performed by a two-step procedure, reverse transcription and PCR.

    Article Title: DIFFERENCES IN CYSTEINE PROTEASE ACTIVITY IN SCHISTOSOMA MANSONI-RESISTANT AND -SUSCEPTIBLE BIOMPHALARIA GLABRATA AND CHARACTERIZATION OF THE HEPATOPANCREAS CATHEPSIN B FULL-LENGTH cDNA
    Article Snippet: .. The RT-PCR reactions were performed using an Applied Biosystems 7300 Real-Time PCR System (Applied Biosystems, Foster City, California). ..

    Quantitative RT-PCR:

    Article Title: Knockdown of CypA inhibits interleukin-8 (IL-8) and IL-8-mediated proliferation and tumor growth of glioblastoma cells through down-regulated NF-?B
    Article Snippet: .. Quantitative real-time PCR (QRT-PCR) was performed using the Power SYBR Green PCR Master Mix (Applied Biosystem) and detected by the 7300 Real Time PCR System (Applied Biosystem). .. The relative expression of IL -8 mRNA normalized to the internal reference 18S rRNA was analyzed using the 2T −ΔΔC method [ ].

    Article Title: Ralstonia solanacearum promotes pathogenicity by utilizing l‐glutamic acid from host plants, et al. Ralstonia solanacearum promotes pathogenicity by utilizing l‐glutamic acid from host plants
    Article Snippet: .. HTSeq v. 0.6.1 was used to count the read numbers mapped to each gene, and the fragments per kilobase of transcript per million reads mapped (FPKM) of each gene was calculated based on the length of the gene and read counts mapped to the gene (Trapnell et al., ). cDNA synthesis and RT‐qPCR analysis were performed with the ChamQ Universal SYBR qPCR Master Mix (Vazyme) on a 7300 Plus real‐time PCR system (Applied Biosystems) following the manufacturer's instructions. .. The gene expression level of recA was used as a control for RT‐qPCR analysis.

    Article Title: Fibroblast mTOR/PPARγ/HGF axis protects against tubular cell death and acute kidney injury
    Article Snippet: .. Real-time qRT-PCR assay was used to quantitate the mRNA abundance with 7300 real-time PCR system (Applied Biosystems, Foster City, CA). ..

    Article Title: Divergent Roles for Airway Epithelial MMP7 and Retinoic Acid in Experimental Asthma
    Article Snippet: .. One step real-time quantitative RT-PCR was used to determine the relative expression of Ccl11 (eotaxin-1) and Raldh-1 in Real-Time PCR System (7300 Applied Biosystems). .. The primer and probe mix of target genes, (Ccl11 : Mm00441238_m1, Raldh-1 : Mm01194995_mH) and 18S rRNA were purchased from Applied Biosystems.

    SYBR Green Assay:

    Article Title: Knockdown of CypA inhibits interleukin-8 (IL-8) and IL-8-mediated proliferation and tumor growth of glioblastoma cells through down-regulated NF-?B
    Article Snippet: .. Quantitative real-time PCR (QRT-PCR) was performed using the Power SYBR Green PCR Master Mix (Applied Biosystem) and detected by the 7300 Real Time PCR System (Applied Biosystem). .. The relative expression of IL -8 mRNA normalized to the internal reference 18S rRNA was analyzed using the 2T −ΔΔC method [ ].

    ALP Assay:

    Article Title: Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration
    Article Snippet: .. To analyze the expression of specific genes for osteoblastic differentiation, the expression of Runx2, osterix, ALP, collagen I, and osteopontin (OPN) was determined quantitatively on a real-time PCR machine (ABI 7300; Applied Biosystems, Foster City, CA, ) using a SYBR Premix Ex Taq kit (Takara), with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the reference gene for normalization. .. The expression of E-cadherin, vimentin, Klf4, Snai1, transforming growth factor-β (TGF-β) receptor type 1, and TGF-β1, key genes involved in epithelial-mesenchymal transformation (EMT) of hAECs [ ], were also determined quantitatively.

    Expressing:

    Article Title: Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration
    Article Snippet: .. To analyze the expression of specific genes for osteoblastic differentiation, the expression of Runx2, osterix, ALP, collagen I, and osteopontin (OPN) was determined quantitatively on a real-time PCR machine (ABI 7300; Applied Biosystems, Foster City, CA, ) using a SYBR Premix Ex Taq kit (Takara), with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the reference gene for normalization. .. The expression of E-cadherin, vimentin, Klf4, Snai1, transforming growth factor-β (TGF-β) receptor type 1, and TGF-β1, key genes involved in epithelial-mesenchymal transformation (EMT) of hAECs [ ], were also determined quantitatively.

    Article Title: Divergent Roles for Airway Epithelial MMP7 and Retinoic Acid in Experimental Asthma
    Article Snippet: .. One step real-time quantitative RT-PCR was used to determine the relative expression of Ccl11 (eotaxin-1) and Raldh-1 in Real-Time PCR System (7300 Applied Biosystems). .. The primer and probe mix of target genes, (Ccl11 : Mm00441238_m1, Raldh-1 : Mm01194995_mH) and 18S rRNA were purchased from Applied Biosystems.

    Polymerase Chain Reaction:

    Article Title: Knockdown of CypA inhibits interleukin-8 (IL-8) and IL-8-mediated proliferation and tumor growth of glioblastoma cells through down-regulated NF-?B
    Article Snippet: .. Quantitative real-time PCR (QRT-PCR) was performed using the Power SYBR Green PCR Master Mix (Applied Biosystem) and detected by the 7300 Real Time PCR System (Applied Biosystem). .. The relative expression of IL -8 mRNA normalized to the internal reference 18S rRNA was analyzed using the 2T −ΔΔC method [ ].

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    Thermo Fisher real time qpcr quantification
    Quantitative PCR <t>(qPCR)</t> validation of miRNA microarray data. The expression of selected <t>miRNAs</t> was verified with real-time PCR. A : relative fold changes (log 2 EOM/TA). B : comparison of fold changes seen in the microarrays and by qPCR. Changes were correlated, significantly different, and concordant in terms of expression levels. Means ± SE; n = 3. P
    Real Time Qpcr Quantification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time qpcr quantification/product/Thermo Fisher
    Average 99 stars, based on 205 article reviews
    Price from $9.99 to $1999.99
    real time qpcr quantification - by Bioz Stars, 2021-01
    99/100 stars
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    99
    Thermo Fisher real time pcr system
    Real time <t>RT-PCR</t> analysis of (A) OPN, (B) OCN, and (C) <t>ALP</t> gene expressions on day 21 with the application of a moving magnetic field. GAPDH was used as the housekeeping gene. The representation “+” means applied the moving magnetic field.
    Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 1626 article reviews
    Price from $9.99 to $1999.99
    real time pcr system - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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    Quantitative PCR (qPCR) validation of miRNA microarray data. The expression of selected miRNAs was verified with real-time PCR. A : relative fold changes (log 2 EOM/TA). B : comparison of fold changes seen in the microarrays and by qPCR. Changes were correlated, significantly different, and concordant in terms of expression levels. Means ± SE; n = 3. P

    Journal: Physiological Genomics

    Article Title: Distinctive patterns of microRNA expression in extraocular muscles

    doi: 10.1152/physiolgenomics.00169.2009

    Figure Lengend Snippet: Quantitative PCR (qPCR) validation of miRNA microarray data. The expression of selected miRNAs was verified with real-time PCR. A : relative fold changes (log 2 EOM/TA). B : comparison of fold changes seen in the microarrays and by qPCR. Changes were correlated, significantly different, and concordant in terms of expression levels. Means ± SE; n = 3. P

    Article Snippet: Reverse transcription and real-time qPCR quantification and validation of miRNAs.

    Techniques: Real-time Polymerase Chain Reaction, Microarray, Expressing

    Classical hematoxylin and eosin (H E) staining (a) and SMARCB1 immunhistochemistry (b) of a biphasic synovial sarcoma. Epithelial components show positive reaction with SMARCB1 immunostaining, while the spindle cell components are negatively stained (b). miR-206 is highly overexpressed (37,66-fold) in the spindle cell components compared to the epithelial components, measured with q-RT-PCR. Relative miR-206 level was normalized to endogenous RNU6B. As calibrator, normal liver tissue was used (c). (A color version of this figure is available in the online journal.)

    Journal: Experimental Biology and Medicine

    Article Title: The oncomir face of microRNA-206: A permanent miR-206 transfection study

    doi: 10.1177/1535370218795406

    Figure Lengend Snippet: Classical hematoxylin and eosin (H E) staining (a) and SMARCB1 immunhistochemistry (b) of a biphasic synovial sarcoma. Epithelial components show positive reaction with SMARCB1 immunostaining, while the spindle cell components are negatively stained (b). miR-206 is highly overexpressed (37,66-fold) in the spindle cell components compared to the epithelial components, measured with q-RT-PCR. Relative miR-206 level was normalized to endogenous RNU6B. As calibrator, normal liver tissue was used (c). (A color version of this figure is available in the online journal.)

    Article Snippet: The thermal parameters were the same as described above in part of real-time PCR quantification of miR-206.

    Techniques: Staining, Immunostaining, Reverse Transcription Polymerase Chain Reaction

    Real time RT-PCR analysis of (A) OPN, (B) OCN, and (C) ALP gene expressions on day 21 with the application of a moving magnetic field. GAPDH was used as the housekeeping gene. The representation “+” means applied the moving magnetic field.

    Journal: PLoS ONE

    Article Title: Injectable polypeptide hydrogel/inorganic nanoparticle composites for bone tissue engineering

    doi: 10.1371/journal.pone.0210285

    Figure Lengend Snippet: Real time RT-PCR analysis of (A) OPN, (B) OCN, and (C) ALP gene expressions on day 21 with the application of a moving magnetic field. GAPDH was used as the housekeeping gene. The representation “+” means applied the moving magnetic field.

    Article Snippet: Three different genes were analyzed including alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN) using a real-time PCR system (ABI Prism 7300, Thermo Fisher, USA).

    Techniques: Quantitative RT-PCR, ALP Assay