Journal: BMC Genomic Data

Article Title: Whole-genome sequence of Pseudomonas sp. strain HOU2 isolated from dangshen ( Codonopsis javanica ) roots

doi: 10.1186/s12863-024-01291-1

Figure Lengend Snippet: Overview of data files/ sets

Article Snippet: The Pseudomonas sp. strain HOU2 genome was determined to be most similar to that of Pseudomonas koreensis using the Type Strain Genome Server ( https://tygs.dsmz.de/ , version v391) [ ].

Techniques: Sequencing

Journal: Frontiers in Microbiology

Article Title: Diversity and functional traits based indigenous rhizosphere associated phosphate solubilizing bacteria for sustainable production of rice

doi: 10.3389/fmicb.2024.1470019

Figure Lengend Snippet: Soil microcosm to study the effect of Acinetobacter sp. MR5 and Pseudomonas sp. MR7 at 15 DPI, 30 DPI, and 60 DPI on (A) soil available P (B) bacterial phosphatase activity and (C) total bacterial population. Means are an average of three biological replicates and means followed by the same letter differ non-significantly at p = 0.01 according to LSD using 3-way ANOVA.

Article Snippet: Bacterial strains Acinetobacter sp. strain MR5 (GenBank accession number LT629134; DSMZ accession number 106631) and Pseudomonas sp. strain MR7 (GenBank accession number LT629136; DSMZ accession number 106634), were isolated from rice-growing areas in Punjab, Pakistan, and selected based on their phosphate-solubilizing potential ( ).

Techniques: Activity Assay

Journal: Frontiers in Microbiology

Article Title: Diversity and functional traits based indigenous rhizosphere associated phosphate solubilizing bacteria for sustainable production of rice

doi: 10.3389/fmicb.2024.1470019

Figure Lengend Snippet: The interaction of different variables in response to inoculation of Acinetobacter sp. MR5 and Pseudomonas sp. MR7 on solubilized phosphate. 3D surface curves were plotted against two independent variables and keeping other variable at their central (0) level. The 3D curves and contour plots from the interactions between variables of the calculated response are (A,D) : relative effect of pH and temperature on phosphate solubilization while keeping inoculum concentration at its central level. (B,E) relative effect of temperature and inoculum size on phosphate solubilization while keeping temperature at its central level. (C,F) relative effect of pH and inoculum size on phosphate solubilization while keeping pH at its central level.

Article Snippet: Bacterial strains Acinetobacter sp. strain MR5 (GenBank accession number LT629134; DSMZ accession number 106631) and Pseudomonas sp. strain MR7 (GenBank accession number LT629136; DSMZ accession number 106634), were isolated from rice-growing areas in Punjab, Pakistan, and selected based on their phosphate-solubilizing potential ( ).

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Diversity and functional traits based indigenous rhizosphere associated phosphate solubilizing bacteria for sustainable production of rice

doi: 10.3389/fmicb.2024.1470019

Figure Lengend Snippet: Biokinetics to study the phosphate solubilization activity of two Acinetobacter sp. MR5 and Pseudomonas sp. MR7. Effect of inoculum on (A) Specific growth rate. (B) P solubilization (C) effect of inoculum on pH. Acinetobacter sp. MR5 (0.022 and 0.038% inoculum w/v wet cells) and Pseudomonas sp. MR7 (0.008 and 0.014% inoculum w/v wet cells) were used for inoculation in Pikovskaya broth and kept at 36 ± 2°C. The supernatant was collected at different time intervals (4, 12, 18, 22, and 34 h) and plotted against the time for activity. Means are an average of three biological replicates, ± represents standard deviation.

Article Snippet: Bacterial strains Acinetobacter sp. strain MR5 (GenBank accession number LT629134; DSMZ accession number 106631) and Pseudomonas sp. strain MR7 (GenBank accession number LT629136; DSMZ accession number 106634), were isolated from rice-growing areas in Punjab, Pakistan, and selected based on their phosphate-solubilizing potential ( ).

Techniques: Activity Assay, Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Diversity and functional traits based indigenous rhizosphere associated phosphate solubilizing bacteria for sustainable production of rice

doi: 10.3389/fmicb.2024.1470019

Figure Lengend Snippet: Re-isolation of inoculated PSB colonies (A) . Plate showing re-isolated colonies of MR5 and MR7 (B, C) . Confocal Laser Scanning Microscopy of rice roots at 35 days post inoculation of P solubilizing bacteria in the microplot experiment under net house conditions. Control treatments without inoculation (D) and from the PSB inoculation of MR5 (E) and MR7 (F) . Plant growth-promoting attributes of re-isolated PSB strains for confirmation of inoculated PSB: P solubilization and IAA production were detected quantitatively by high-performance liquid chromatography (HPLC), gluconic acid production and siderophores production (G) . Values are an average of six biological replicates. MR5: Acinetobacter sp.’ MR7 and Pseudomonas sp. The photograph of the gel indicates re-isolated colonies of PSB that are similar morphologically to the inoculated consortium (H) .

Article Snippet: Bacterial strains Acinetobacter sp. strain MR5 (GenBank accession number LT629134; DSMZ accession number 106631) and Pseudomonas sp. strain MR7 (GenBank accession number LT629136; DSMZ accession number 106634), were isolated from rice-growing areas in Punjab, Pakistan, and selected based on their phosphate-solubilizing potential ( ).

Techniques: Isolation, Confocal Laser Scanning Microscopy, Bacteria, Control, High Performance Liquid Chromatography