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medium salt active nuclease  (ArcticZymes)


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    ArcticZymes medium salt active nuclease
    Medium Salt Active Nuclease, supplied by ArcticZymes, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/medium salt active nuclease/product/ArcticZymes
    Average 94 stars, based on 1 article reviews
    medium salt active nuclease - by Bioz Stars, 2025-03
    94/100 stars

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    4D contour plot of the DNA concentrations in ng∙mL −1 <t>after</t> <t>nuclease</t> digestion. Treated material was (A) crude harvest and (B) supernatant with <t>M-SAN</t> HQ (first column), SAN HQ (second column), and Denarase® (third column) in dependence of the factors incubation time in h (x-axis), nuclease concentration in U∙mL −1 (y-axis) and incubation temperature (first row 4 °C, second row, 23 °C, third row 37 °C).
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    Image Search Results


    4D contour plot of the DNA concentrations in ng∙mL −1 after nuclease digestion. Treated material was (A) crude harvest and (B) supernatant with M-SAN HQ (first column), SAN HQ (second column), and Denarase® (third column) in dependence of the factors incubation time in h (x-axis), nuclease concentration in U∙mL −1 (y-axis) and incubation temperature (first row 4 °C, second row, 23 °C, third row 37 °C).

    Journal: Vaccine: X

    Article Title: Efficient and scalable clarification of Orf virus from HEK suspension for vaccine development

    doi: 10.1016/j.jvacx.2024.100474

    Figure Lengend Snippet: 4D contour plot of the DNA concentrations in ng∙mL −1 after nuclease digestion. Treated material was (A) crude harvest and (B) supernatant with M-SAN HQ (first column), SAN HQ (second column), and Denarase® (third column) in dependence of the factors incubation time in h (x-axis), nuclease concentration in U∙mL −1 (y-axis) and incubation temperature (first row 4 °C, second row, 23 °C, third row 37 °C).

    Article Snippet: Nuclease (Denarase® (c-LEcta), M-SAN HQ, and SAN HQ (both ArcticZymes)), iv.

    Techniques: Incubation, Concentration Assay

    Filter screening at different times of harvest. ORFV yield, turbidity at OD400, and dsDNA concentration after clarification with Sartopure® PP3 membranes of different pore sizes (A, C, E) without nuclease treatment (negative control) or (B, D, F) with M-SAN HQ treatment. The filtration was performed after three different times of harvest (TOH), which were 40, 70, and 90 h (±4h for each) post-infection.

    Journal: Vaccine: X

    Article Title: Efficient and scalable clarification of Orf virus from HEK suspension for vaccine development

    doi: 10.1016/j.jvacx.2024.100474

    Figure Lengend Snippet: Filter screening at different times of harvest. ORFV yield, turbidity at OD400, and dsDNA concentration after clarification with Sartopure® PP3 membranes of different pore sizes (A, C, E) without nuclease treatment (negative control) or (B, D, F) with M-SAN HQ treatment. The filtration was performed after three different times of harvest (TOH), which were 40, 70, and 90 h (±4h for each) post-infection.

    Article Snippet: Nuclease (Denarase® (c-LEcta), M-SAN HQ, and SAN HQ (both ArcticZymes)), iv.

    Techniques: Concentration Assay, Clarification Assay, Negative Control, Filtration, Infection

    Summary of the time of harvest (TOH) choice and use of a nuclease. Influence of the time of harvest (TOH) which was 40, 70, and 90 h (±4h for each) post-infection on the aim to achieve a high ORFV yield, a low final turbidity, a low DNA concentration in the filtrate, as well as a high filter capacity. The influence of using M-SAN HQ on these four responses was additionally evaluated. Color code: green = advantageous/good, yellow = medium/ok, and red = disadvantageous to achieve the desired results of the analyzed factors. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Vaccine: X

    Article Title: Efficient and scalable clarification of Orf virus from HEK suspension for vaccine development

    doi: 10.1016/j.jvacx.2024.100474

    Figure Lengend Snippet: Summary of the time of harvest (TOH) choice and use of a nuclease. Influence of the time of harvest (TOH) which was 40, 70, and 90 h (±4h for each) post-infection on the aim to achieve a high ORFV yield, a low final turbidity, a low DNA concentration in the filtrate, as well as a high filter capacity. The influence of using M-SAN HQ on these four responses was additionally evaluated. Color code: green = advantageous/good, yellow = medium/ok, and red = disadvantageous to achieve the desired results of the analyzed factors. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Nuclease (Denarase® (c-LEcta), M-SAN HQ, and SAN HQ (both ArcticZymes)), iv.

    Techniques: Infection, Concentration Assay