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salt active nuclease  (ArcticZymes)


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    ArcticZymes salt active nuclease
    Salt Active Nuclease, supplied by ArcticZymes, used in various techniques. Bioz Stars score: 95/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/salt active nuclease/product/ArcticZymes
    Average 95 stars, based on 82 article reviews
    salt active nuclease - by Bioz Stars, 2026-06
    95/100 stars

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    Overview of the experimental design. (A) Testing different concentrations of saponin (2%–5%) to observe the lysis of host cells and bacterial cells. (B) Host DNA depletion of Escherichia coli and Staphylococcus aureus blood cultures using saponin and various concentrations of salt-activated <t>endonucleases</t> <t>(SAN),</t> followed by DNA extraction. (C) Assessing the effect of different salt concentrations on SAN activity. (D) The final optimized protocol uses 4% saponin, 10 μL (250 U) of <t>HL-SAN,</t> 2.5 M NaCl, and 50 mM MgCl 2 for effective host DNA depletion. * indicates methodology where the following concentrations were tested: 2 vs. 2.5 M NaCl + 50 mM MgCl 2 , 2.5 M NaCl + 15 vs. 50 mM MgCl 2 , 0.5 M NaCl + 15 mM MgCl 2 vs. 2.5 M NaCl + 50 mM MgCl 2 (Created with BioRender.com ).
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    Overview of the experimental design. (A) Testing different concentrations of saponin (2%–5%) to observe the lysis of host cells and bacterial cells. (B) Host DNA depletion of Escherichia coli and Staphylococcus aureus blood cultures using saponin and various concentrations of salt-activated endonucleases (SAN), followed by DNA extraction. (C) Assessing the effect of different salt concentrations on SAN activity. (D) The final optimized protocol uses 4% saponin, 10 μL (250 U) of HL-SAN, 2.5 M NaCl, and 50 mM MgCl 2 for effective host DNA depletion. * indicates methodology where the following concentrations were tested: 2 vs. 2.5 M NaCl + 50 mM MgCl 2 , 2.5 M NaCl + 15 vs. 50 mM MgCl 2 , 0.5 M NaCl + 15 mM MgCl 2 vs. 2.5 M NaCl + 50 mM MgCl 2 (Created with BioRender.com ).

    Journal: Frontiers in Microbiology

    Article Title: Development and optimization of host DNA depletion in blood cultures using a saponin and salt-activated nuclease-based method

    doi: 10.3389/fmicb.2026.1784408

    Figure Lengend Snippet: Overview of the experimental design. (A) Testing different concentrations of saponin (2%–5%) to observe the lysis of host cells and bacterial cells. (B) Host DNA depletion of Escherichia coli and Staphylococcus aureus blood cultures using saponin and various concentrations of salt-activated endonucleases (SAN), followed by DNA extraction. (C) Assessing the effect of different salt concentrations on SAN activity. (D) The final optimized protocol uses 4% saponin, 10 μL (250 U) of HL-SAN, 2.5 M NaCl, and 50 mM MgCl 2 for effective host DNA depletion. * indicates methodology where the following concentrations were tested: 2 vs. 2.5 M NaCl + 50 mM MgCl 2 , 2.5 M NaCl + 15 vs. 50 mM MgCl 2 , 0.5 M NaCl + 15 mM MgCl 2 vs. 2.5 M NaCl + 50 mM MgCl 2 (Created with BioRender.com ).

    Article Snippet: The host DNA depletion in blood cultures was carried out by treating the blood cultures with 4% saponin, followed by the addition of HL-SAN (Catalog no. 70910-202) and M-SAN (Catalog no. 70950-202) (ArcticZymes Technologies, Norway).

    Techniques: Lysis, DNA Extraction, Activity Assay

    Effect of different enzyme concentrations on host DNA depletion evaluated using qPCR Ct values. (A) Escherichia coli (B) Staphylococcus aureus . HL-10 / HL-20 = 10 or 20 μL (250/500 U) HL-SAN, M-10 / M-20 = 10 or 20 μL (250/500 U) M-SAN.

    Journal: Frontiers in Microbiology

    Article Title: Development and optimization of host DNA depletion in blood cultures using a saponin and salt-activated nuclease-based method

    doi: 10.3389/fmicb.2026.1784408

    Figure Lengend Snippet: Effect of different enzyme concentrations on host DNA depletion evaluated using qPCR Ct values. (A) Escherichia coli (B) Staphylococcus aureus . HL-10 / HL-20 = 10 or 20 μL (250/500 U) HL-SAN, M-10 / M-20 = 10 or 20 μL (250/500 U) M-SAN.

    Article Snippet: The host DNA depletion in blood cultures was carried out by treating the blood cultures with 4% saponin, followed by the addition of HL-SAN (Catalog no. 70910-202) and M-SAN (Catalog no. 70950-202) (ArcticZymes Technologies, Norway).

    Techniques:

    The effect of MgCl 2 concentrations on HL-SAN and M-SAN activity for host DNA depletion. Host DNA depletion and bacterial DNA enrichment were assessed using ΔCt values, normalized to the control samples. These normalized Ct values were converted to fold changes and were represented on a log scale to improve visualization. The 50 mM MgCl 2 concentration is used as a reference for comparison with the 15 mM MgCl 2 . The concentration of NaCl is kept constant at 2.5 M (A) Escherichia coli and (B) Staphylococcus aureus . 15 mM/50 mM = MgCl 2 . All test values are presented as mean ± SD, with n = 2 biological replicates for each condition. ns > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

    Journal: Frontiers in Microbiology

    Article Title: Development and optimization of host DNA depletion in blood cultures using a saponin and salt-activated nuclease-based method

    doi: 10.3389/fmicb.2026.1784408

    Figure Lengend Snippet: The effect of MgCl 2 concentrations on HL-SAN and M-SAN activity for host DNA depletion. Host DNA depletion and bacterial DNA enrichment were assessed using ΔCt values, normalized to the control samples. These normalized Ct values were converted to fold changes and were represented on a log scale to improve visualization. The 50 mM MgCl 2 concentration is used as a reference for comparison with the 15 mM MgCl 2 . The concentration of NaCl is kept constant at 2.5 M (A) Escherichia coli and (B) Staphylococcus aureus . 15 mM/50 mM = MgCl 2 . All test values are presented as mean ± SD, with n = 2 biological replicates for each condition. ns > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

    Article Snippet: The host DNA depletion in blood cultures was carried out by treating the blood cultures with 4% saponin, followed by the addition of HL-SAN (Catalog no. 70910-202) and M-SAN (Catalog no. 70950-202) (ArcticZymes Technologies, Norway).

    Techniques: Activity Assay, Control, Concentration Assay, Comparison

    The effect of NaCl and MgCl 2 concentrations on HL-SAN and M-SAN activity for optimal host DNA depletion. Host DNA depletion and bacterial DNA enrichment were assessed using ΔCt values, normalized to the control samples. These normalized Ct values were converted to fold changes and were represented on a log scale to improve visualization. The 2.5 M NaCl and 50 mM MgCl 2 concentrations are used as reference control and are compared with 0.5 M NaCl 15 mM MgCl 2 . (A) E. coli (B) S. aureus . HL_SAN/M_SAN = 0.5 M NaCl and 15 mM MgCl 2 , HL_SAN/M_SAN control = 2.5 M NaCl and 50 mM MgCl 2 . All test values are presented as mean ± SD, with n = 2 biological replicates for each condition. ns > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

    Journal: Frontiers in Microbiology

    Article Title: Development and optimization of host DNA depletion in blood cultures using a saponin and salt-activated nuclease-based method

    doi: 10.3389/fmicb.2026.1784408

    Figure Lengend Snippet: The effect of NaCl and MgCl 2 concentrations on HL-SAN and M-SAN activity for optimal host DNA depletion. Host DNA depletion and bacterial DNA enrichment were assessed using ΔCt values, normalized to the control samples. These normalized Ct values were converted to fold changes and were represented on a log scale to improve visualization. The 2.5 M NaCl and 50 mM MgCl 2 concentrations are used as reference control and are compared with 0.5 M NaCl 15 mM MgCl 2 . (A) E. coli (B) S. aureus . HL_SAN/M_SAN = 0.5 M NaCl and 15 mM MgCl 2 , HL_SAN/M_SAN control = 2.5 M NaCl and 50 mM MgCl 2 . All test values are presented as mean ± SD, with n = 2 biological replicates for each condition. ns > 0.05; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001.

    Article Snippet: The host DNA depletion in blood cultures was carried out by treating the blood cultures with 4% saponin, followed by the addition of HL-SAN (Catalog no. 70910-202) and M-SAN (Catalog no. 70950-202) (ArcticZymes Technologies, Norway).

    Techniques: Activity Assay, Control