Journal: AMB Express

Article Title: Characterization of a newly isolated biosurfactant fengycin produced by Heyndrickxia coagulans strain

doi: 10.1186/s13568-025-01942-1

Figure Lengend Snippet: UHPLC chromatograms of biosurfactant isolated from H. coagulans 9FT27 ( A ) and fengycin standard ( B )

Article Snippet: The strain B. coagulans Hammer (ATCC 7050; DSM1) was first described as the type strain of B. coagulans in 1915 by Hammer, who isolated this organism from spoiled canned milk (De Clerck et al. ).

Techniques: Isolation

Journal: AMB Express

Article Title: Characterization of a newly isolated biosurfactant fengycin produced by Heyndrickxia coagulans strain

doi: 10.1186/s13568-025-01942-1

Figure Lengend Snippet: 1 H NMR spectrum of the fengycin isolate of H. coagulans 9FT27, 2D TOCSY spectrum of the spin system belonging to the fatty acid chain ( A ) measured in DMSO- d 6

Article Snippet: The strain B. coagulans Hammer (ATCC 7050; DSM1) was first described as the type strain of B. coagulans in 1915 by Hammer, who isolated this organism from spoiled canned milk (De Clerck et al. ).

Techniques:

Journal: AMB Express

Article Title: Characterization of a newly isolated biosurfactant fengycin produced by Heyndrickxia coagulans strain

doi: 10.1186/s13568-025-01942-1

Figure Lengend Snippet: MALDI-TOF/MS spectra of fengycin isolated from H. coagulans 9FT27 and commercial fengycin standard

Article Snippet: The strain B. coagulans Hammer (ATCC 7050; DSM1) was first described as the type strain of B. coagulans in 1915 by Hammer, who isolated this organism from spoiled canned milk (De Clerck et al. ).

Techniques: Isolation

Journal: bioRxiv

Article Title: Resolving the estrogen paradox in hereditary retinal degeneration: Esr1 activation suppresses Tnf-α signaling as a photoreceptor self-protection mechanism

doi: 10.1101/2025.09.07.674682

Figure Lengend Snippet: Esr1 activation suppresses Tnf-α signaling to protect photoreceptors. (A) GSEA of rd1 retinal RNA-seq from explants showed negative enrichment of Tnf associated pathways after PPT treatment. (B) CytoHubba analysis identified Tnf as a common hub gene post-PPT. (C) Tnf expression (FPKM) is significantly reduced by PPT. (D-K) Immunostaining for Tnf-α (green) reveals elevated signal in rd1 and rd10 versus wt ; PPT reduces Tnf-α in explant and in vivo models (D-G). ZAP elevated Tnf-α in wt , which was reversed by PPT (H-K). Notably, Tnf-α localized predominantly to Müller cells. (L-M) TUNEL assays (magenta) with DAPI (grey) as nuclear counterstain in rd1 explants showed decreased ONL cell death with Tnf-α blockade (ETN, IFX) and further protection by the Tnf receptor antagonist R7050. (N-Q) Intravitreal ETN in rd10 reduced ONL TUNEL positivity (N,O) and restored dark-adapted ERG a-wave amplitudes (P,Q). Error bars: SD; significance levels: * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001. OS = outer segment, IS = inner segment, ONL = outer nuclear layer, INL = inner nuclear layer, IPL = inner plexiform layer, GCL = ganglion cell layer, NFL = nerve fiber layer; scale bar = 50 µm. Dashed lines indicate untreated levels.

Article Snippet: After 48h in culture, explants were treated with 10μM E2 [ ] (HY-B0141; MedChemExpress, Sollentuna, Sweden), 10μM E2 plus 10nM AZD9496 [ ] (HY-12870; MedChemExpress), 15nM PPT [ ] (HY-100689; MedChemExpress), 0.63nM Etanercept [ ] (ETN, HY-108847; MedChemExpress), 6.7μM Infliximab [ ] (IFX, HY-P9970; MedChemExpress), 65μM R7050 [ ] (HY-110203; MedChemExpress), or 4μM AZD8797 [ ] (HY-13848; MedChemExpress).

Techniques: Activation Assay, RNA Sequencing, Expressing, Immunostaining, In Vivo, TUNEL Assay

Journal: bioRxiv

Article Title: Resolving the estrogen paradox in hereditary retinal degeneration: Esr1 activation suppresses Tnf-α signaling as a photoreceptor self-protection mechanism

doi: 10.1101/2025.09.07.674682

Figure Lengend Snippet: Tnf-α and Tnfr blockade protect photoreceptors and restore retinal function. (A-D) ETN reduced ZAP-induced ONL TUNEL positivity (A,B) and restored dark-adapted ERG responses (C) and a-wave amplitudes (D) in wt . (E-H) IFX lowered ONL TUNEL-positive cells in rd10 (E,F) and improved ERG responses (G,H). (I-L) IFX also mitigated ZAP-induced ONL death (I,J) and rescued ERG function (K,L) in wt . (M-P) R7050 markedly decreased ONL TUNEL positivity in rd10 (M,N) and enhanced ERG responses (O,P). (Q-T) R7050 further reduced ZAP-induced retinal degeneration in wt (Q,R) and restored dark-adapted ERG responses (S) and a-wave amplitudes (T). Error bars: SD; significance levels: * = p < 0.05; ** = p < 0.01;. ONL = outer nuclear layer, INL = inner nuclear layer, GCL = ganglion cell layer, scale bar = 50 µm; TUNEL assays (magenta) with DAPI (grey) as nuclear counterstain.

Article Snippet: After 48h in culture, explants were treated with 10μM E2 [ ] (HY-B0141; MedChemExpress, Sollentuna, Sweden), 10μM E2 plus 10nM AZD9496 [ ] (HY-12870; MedChemExpress), 15nM PPT [ ] (HY-100689; MedChemExpress), 0.63nM Etanercept [ ] (ETN, HY-108847; MedChemExpress), 6.7μM Infliximab [ ] (IFX, HY-P9970; MedChemExpress), 65μM R7050 [ ] (HY-110203; MedChemExpress), or 4μM AZD8797 [ ] (HY-13848; MedChemExpress).

Techniques: TUNEL Assay

Journal: bioRxiv

Article Title: Resolving the estrogen paradox in hereditary retinal degeneration: Esr1 activation suppresses Tnf-α signaling as a photoreceptor self-protection mechanism

doi: 10.1101/2025.09.07.674682

Figure Lengend Snippet: Esr1 activation, Tnf-α inhibition, and Cx3cr1 blockade suppress microglial recruitment and Cx3cl1/Cx3cr1 signaling in rd10 . (A–B) Immunostaining of Iba1 revealed robust microglial recruitment into the ONL in rd10 in vivo, which was significantly reduced by Esr1 activation (PPT), Tnf-α blockade (ETN, IFX), Tnfr1 antagonism (R7050), and Cx3cr1 inhibition (AZD8797). (C-D) Cd68 Immunostaining followed a similar trend: minimal in untreated wt , upregulated in rd10 , and attenuated following intervention. (E–H) Immunostaining showed that Cx3cr1 (red) and Cx3cl1 (yellow) expression, barely detectable in wt retinas, were markedly increased in rd10 and suppressed by all treatments. * = p < 0.05; ** = p < 0.01; *** = p < 0.001; **** = p < 0.0001. ONL = outer nuclear layer, INL = inner nuclear layer, GCL = ganglion cell layer, scale bar = 50 µm. Dashed lines indicate expression levels in untreated rd10 controls. DAPI (grey) was used as nuclear counterstain. Statistical testing: one-way ANOVA with Tukey’s multiple comparison post hoc test.

Article Snippet: After 48h in culture, explants were treated with 10μM E2 [ ] (HY-B0141; MedChemExpress, Sollentuna, Sweden), 10μM E2 plus 10nM AZD9496 [ ] (HY-12870; MedChemExpress), 15nM PPT [ ] (HY-100689; MedChemExpress), 0.63nM Etanercept [ ] (ETN, HY-108847; MedChemExpress), 6.7μM Infliximab [ ] (IFX, HY-P9970; MedChemExpress), 65μM R7050 [ ] (HY-110203; MedChemExpress), or 4μM AZD8797 [ ] (HY-13848; MedChemExpress).

Techniques: Activation Assay, Inhibition, Immunostaining, In Vivo, Expressing, Comparison