Journal: BMC Microbiology

Article Title: Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells

doi: 10.1186/1471-2180-12-103

Figure Lengend Snippet: Adherence patterns of O157 strain EDL 933 on RSE cells, in the presence of D + Mannose and +/− antisera. Panel A , in the presence of “pooled antisera” against LEE, Intimin and flagellar H7 proteins, and the anti-Intimin antisera alone, at 1:5 dilutions. Panel B , in the absence of any sera (No sera). The immunofluorescence (IF) stained slides are shown at 40x magnification. O157 have green fluorescence, cytokeratins’ of RSE cells have orange-red fluorescence, and their nuclei have blue fluorescence. The arrows in the adjacent toluidine blue (TB) stained slides, at 40x magnification, point to RSE-adherent O157.

Article Snippet: These protein databases ( Escherichia coli (strain Sakai/O157:H7/RIMD 0509952/EHEC) – Tax ID: 386585 and Escherichia coli (strain EDL933/ATCC 700927/O157:H7/EHEC) – Tax ID: 155864) have a total of 10,737entries.

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: BMC Microbiology

Article Title: Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells

doi: 10.1186/1471-2180-12-103

Figure Lengend Snippet: Quantitative representation of the adherence patterns of O157 strains EDL 933, and 86–24 along with its mutant derivatives, on RSE and HEp-2 cells. Percent mean ± standard error of mean of cells with adherent bacteria or no bacteria, in the ranges shown in the legend, are depicted in each graph.

Article Snippet: These protein databases ( Escherichia coli (strain Sakai/O157:H7/RIMD 0509952/EHEC) – Tax ID: 386585 and Escherichia coli (strain EDL933/ATCC 700927/O157:H7/EHEC) – Tax ID: 155864) have a total of 10,737entries.

Techniques: Mutagenesis

Journal: BMC Microbiology

Article Title: Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells

doi: 10.1186/1471-2180-12-103

Figure Lengend Snippet: Adherence patterns of O157 strains on HEp-2 cells, in the presence of D + Mannose and +/− antisera. Panel A , O157 strain EDL933, in the presence of “pooled antisera” against LEE. Intimin and flagellar H7 proteins, and the anti-Intimin antisera alone, at 1:100 and 1:10 dilutions, respectively. Panel B , O157 strain EDL933, in the absence of any sera (No sera). Panel C , O157 strain 86–24 (Intimin-positive) and its mutant derivatives, 86-24 eae Δ10 (Intimin-negative), and 86-24 eae Δ10 (pEB310) (Initmin-positive) in the absence of any sera. The immunofluorescence (IF) stained slides are shown at 40x magnification. O157 have green fluorescence, actin filaments of HEp-2 cells have orange-red fluorescence, and their nuclei have blue fluorescence.

Article Snippet: These protein databases ( Escherichia coli (strain Sakai/O157:H7/RIMD 0509952/EHEC) – Tax ID: 386585 and Escherichia coli (strain EDL933/ATCC 700927/O157:H7/EHEC) – Tax ID: 155864) have a total of 10,737entries.

Techniques: Mutagenesis, Immunofluorescence, Staining, Fluorescence

Journal: BMC Microbiology

Article Title: Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells

doi: 10.1186/1471-2180-12-103

Figure Lengend Snippet: Adherence patterns of O157 strain 86–24 (Intimin-positive) and its mutant derivatives, 86-24 eae Δ10 (Intimin-negative) and 86-24 eae Δ10 (pEB310) (Initmin-positive), on RSE cells, in the presence of D + Mannose. The immunofluorescence (IF) stained slides are shown at 40x magnification. O157 have green fluorescence, cytokeratins’ of RSE cells have orange-red fluorescence, and their nuclei have blue fluorescence. The arrows in the adjacent toluidine blue (TB) stained slides, at 40x magnification, point to RSE-adherent O157.

Article Snippet: These protein databases ( Escherichia coli (strain Sakai/O157:H7/RIMD 0509952/EHEC) – Tax ID: 386585 and Escherichia coli (strain EDL933/ATCC 700927/O157:H7/EHEC) – Tax ID: 155864) have a total of 10,737entries.

Techniques: Mutagenesis, Immunofluorescence, Staining, Fluorescence

Journal: BMC Microbiology

Article Title: Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells

doi: 10.1186/1471-2180-12-103

Figure Lengend Snippet: Bioinformatically determined putative adhesins in the O157 DMEM-Proteome

Article Snippet: These protein databases ( Escherichia coli (strain Sakai/O157:H7/RIMD 0509952/EHEC) – Tax ID: 386585 and Escherichia coli (strain EDL933/ATCC 700927/O157:H7/EHEC) – Tax ID: 155864) have a total of 10,737entries.

Techniques: Inhibition, Scaffolding, Binding Assay

Journal: Frontiers in Microbiology

Article Title: Cyanobacteria as cell factories for the photosynthetic production of sucrose

doi: 10.3389/fmicb.2023.1126032

Figure Lengend Snippet: Sucrose-based autotroph-heterotroph co-cultures and their products.

Article Snippet: Similarly, S. elongatus PCC 7942 was originally engineered to export sucrose by expressing sucrose permease ( cscB ) from Escherichia coli ATCC 700927 , and multiple cyanobacterial species have since been similarly modified by different research teams ( ).

Techniques:

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

Techniques: Expressing, Mutagenesis, Labeling

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

Techniques: Expressing, Mutagenesis, Plasmid Preparation, Labeling

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

Techniques: Mutagenesis, Western Blot, SDS Page

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

Techniques: Expressing, Mutagenesis, Labeling

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

Techniques: Infection, Mutagenesis

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

Article Snippet: The ability of EHEC EDL933 (ATCC 700927) wild type and its mutant derivatives to adhere and form A/E lesions on HEp-2 cell monolayers was evaluated using the fluorescent actin staining assay as described [ ].

Techniques: Expressing, Mutagenesis, Labeling

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

Article Snippet: The ability of EHEC EDL933 (ATCC 700927) wild type and its mutant derivatives to adhere and form A/E lesions on HEp-2 cell monolayers was evaluated using the fluorescent actin staining assay as described [ ].

Techniques: Expressing, Mutagenesis, Plasmid Preparation, Labeling

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

Article Snippet: The ability of EHEC EDL933 (ATCC 700927) wild type and its mutant derivatives to adhere and form A/E lesions on HEp-2 cell monolayers was evaluated using the fluorescent actin staining assay as described [ ].

Techniques: Mutagenesis, Western Blot, SDS Page

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

Article Snippet: The ability of EHEC EDL933 (ATCC 700927) wild type and its mutant derivatives to adhere and form A/E lesions on HEp-2 cell monolayers was evaluated using the fluorescent actin staining assay as described [ ].

Techniques: Expressing, Mutagenesis, Labeling

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

Article Snippet: The ability of EHEC EDL933 (ATCC 700927) wild type and its mutant derivatives to adhere and form A/E lesions on HEp-2 cell monolayers was evaluated using the fluorescent actin staining assay as described [ ].

Techniques: Infection, Mutagenesis

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

Techniques: Expressing, Mutagenesis, Labeling

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

Techniques: Expressing, Mutagenesis, Plasmid Preparation, Labeling

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

Techniques: Mutagenesis, Western Blot, SDS Page

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

Techniques: Expressing, Mutagenesis, Labeling

Journal: BMC Microbiology

Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

doi: 10.1186/1471-2180-12-231

Figure Lengend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

Techniques: Infection, Mutagenesis

Journal: PLoS ONE

Article Title: E. coli K-12 and EHEC Genes Regulated by SdiA

doi: 10.1371/journal.pone.0008946

Figure Lengend Snippet: The graphs show the MIC of the wild-type strains and their respective isogenic sdiA mutants (BA612, JNS21, and DL1, respectively). Each bar is the average of two separate experiments performed in triplicate and error bars represent standard deviation.

Article Snippet: 700927 , Wild-type Escherichia coli O157:H7 (EHEC) , ATCC.

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: E. coli K-12 and EHEC Genes Regulated by SdiA

doi: 10.1371/journal.pone.0008946

Figure Lengend Snippet: Strains and Plasmids.

Article Snippet: 700927 , Wild-type Escherichia coli O157:H7 (EHEC) , ATCC.

Techniques: Mutagenesis, Expressing, Plasmid Preparation, Clone Assay

Journal: PLoS ONE

Article Title: E. coli K-12 and EHEC Genes Regulated by SdiA

doi: 10.1371/journal.pone.0008946

Figure Lengend Snippet: Oligonucleotides used.

Article Snippet: 700927 , Wild-type Escherichia coli O157:H7 (EHEC) , ATCC.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: E. coli K-12 and EHEC Genes Regulated by SdiA

doi: 10.1371/journal.pone.0008946

Figure Lengend Snippet: A) Acid fitness island of E. coli . The transposon insertion in E. coli K-12, AL4001, is within gadW at nucleotide 3662317 of Genbank accession number U00096. The transposon insertions in the EHEC strains are shown on the same map but the nucleotide positions are from Genbank accession number BA000007. JLD605 is within gadE at nucleotide 4401036; JLD607 is within yhiD at nucleotide 4397949; JLD610 is within hdeA at nucleotide 4398821. B) JLD604 is just upstream of ECs2675 in the anti-sense orientation at nucleotide 3662317.

Article Snippet: 700927 , Wild-type Escherichia coli O157:H7 (EHEC) , ATCC.

Techniques:

Journal: PLoS ONE

Article Title: E. coli K-12 and EHEC Genes Regulated by SdiA

doi: 10.1371/journal.pone.0008946

Figure Lengend Snippet: Cells were grown in LB glucose with 1 µM oxo-C6 or 0.1% EA at either 37°C or 30°C and then subcultured into pre-warmed MEM with glucose and glutamate at pH 2.0 with continued incubation at the same temperature. Resistance to the acid challenge was determined by plating for cfu/ml every hour for two hours. E. coli K-12 wild-type MG1655 and sdiA mutant JNS21 at 37°C (A) and 30°C (B). EHEC wild-type 700927 and sdiA mutant DL1 at 37°C (C) and 30°C (D). Each strain was assayed in triplicate and error bars represent standard deviation.

Article Snippet: 700927 , Wild-type Escherichia coli O157:H7 (EHEC) , ATCC.

Techniques: Incubation, Mutagenesis, Standard Deviation