Sarstedt
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Sarstedt
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Pfizer Inc
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Routledge Ltd
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Millipore
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Danaher Inc
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Wolters Kluwer Health
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Chemie GmbH
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Thermo Fisher
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Routledge Ltd
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Journal: PLoS Pathogens
Article Title: β-Neurexin Is a Ligand for the Staphylococcus aureus MSCRAMM SdrC
doi: 10.1371/journal.ppat.1000726
Figure Lengend Snippet: ( A ) A cartoon representation of β-neurexin domain structure. S, signal sequence; NL, neuroligin-binding domain; Glyc, glycosylation domain; TM, transmembrane domain; PDZ, intracellular signaling domain. Also shown, are the recombinant proteins used in this study. ( B ) Coomassie-stained SDS-PAGE gel of GST-tagged neurexin 1β domains recombinant proteins. Increasing concentrations of SdrC A ( C ) and SdrC N2N3 ( D ) were incubated with immobilized Nrx 47-255 (black square), Nrx 70–255 (black triangle) or GST (black circle) for 1 hour at room temperature. The apparent K D (concentrations required for half maximum binding) values were 1.85±0.94×10 −7 M for SdrC A and 1.26±0.28×10 −7 M for SdrC N2N3 . ( E ) SdrC 178–496 (0.5 µM) was incubated with increasing concentrations of spNrx wt peptide (black square) or spNrx scr peptide (black triangle) at room temperature. After 2 hours, the mixture was incubated with immobilized Nrx 47–255 . The values represented here are the mean±SD of triplicates from three experiments.
Article Snippet: Plasmids pDEST Nrx 47–255 and
Techniques: Sequencing, Binding Assay, Recombinant, Staining, SDS Page, Incubation
Journal: PLoS Pathogens
Article Title: β-Neurexin Is a Ligand for the Staphylococcus aureus MSCRAMM SdrC
doi: 10.1371/journal.ppat.1000726
Figure Lengend Snippet: Oligonucleotide primers used to amplify gene fragments.
Article Snippet: Plasmids pDEST Nrx 47–255 and
Techniques: