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Shimla Municipal water catchment wildlife sanctuary
Water Catchment Wildlife Sanctuary, supplied by Shimla Municipal, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/water catchment wildlife sanctuary/product/Shimla Municipal
Average 86 stars, based on 1 article reviews
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water catchment wildlife sanctuary - by Bioz Stars, 2024-07
86/100 stars

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Enzo Biochem mt hsp70
( A ) Mitochondrial population abundance was analysed in LINCL cells pre-incubated with (LINCL+TPP-1) or without (LINCL) recombinant TPP-1. Cells were then stained with 100 nM MitoTracker Green for 30 min and fluorescence was detected by flow cytometry as described in the Material and methods section. Results are expressed as MitoTracker Green Fluorescence MCFR calculated from autofluorescence signals measured for corresponding unstained cells and represent means±S.D. ( n =3). NS, non-significantly different from LINCL cells as determined by a Student's t test. ( B ) The abundance of HADHA, β-subunit of ATPsynthase and <t>mt-HSP70</t> was determined by Western blot analysis performed on 15 μg of clear cell lysates prepared from LINCL cells pre-incubated (LINCL+TPP-1) or not (LINCL) with recombinant TPP-1. Equal protein loading was controlled by the immunodetection of β-actin or α-tubulin ( n =3). ( C ) Quantification of the abundance of β-subunit of ATP synthase (grey columns) HADHA (white columns), and mt-HSP70 (black columns) on blots presented in ( B ). Results are calculated as fluorescence intensity normalized for α-tubulin or β-actin and expressed in percentages of LINCL cells as means±S.D. ( n =3). *: significantly different from LINCL cells with P <0.05 as determined by a Student's t test.
Mt Hsp70, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mt hsp70/product/Enzo Biochem
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mt hsp70 - by Bioz Stars, 2024-07
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1) Product Images from "Different molecular mechanisms involved in spontaneous and oxidative stress-induced mitochondrial fragmentation in tripeptidyl peptidase-1 (TPP-1)-deficient fibroblasts"

Article Title: Different molecular mechanisms involved in spontaneous and oxidative stress-induced mitochondrial fragmentation in tripeptidyl peptidase-1 (TPP-1)-deficient fibroblasts

Journal: Bioscience Reports

doi: 10.1042/BSR20120104

( A ) Mitochondrial population abundance was analysed in LINCL cells pre-incubated with (LINCL+TPP-1) or without (LINCL) recombinant TPP-1. Cells were then stained with 100 nM MitoTracker Green for 30 min and fluorescence was detected by flow cytometry as described in the Material and methods section. Results are expressed as MitoTracker Green Fluorescence MCFR calculated from autofluorescence signals measured for corresponding unstained cells and represent means±S.D. ( n =3). NS, non-significantly different from LINCL cells as determined by a Student's t test. ( B ) The abundance of HADHA, β-subunit of ATPsynthase and mt-HSP70 was determined by Western blot analysis performed on 15 μg of clear cell lysates prepared from LINCL cells pre-incubated (LINCL+TPP-1) or not (LINCL) with recombinant TPP-1. Equal protein loading was controlled by the immunodetection of β-actin or α-tubulin ( n =3). ( C ) Quantification of the abundance of β-subunit of ATP synthase (grey columns) HADHA (white columns), and mt-HSP70 (black columns) on blots presented in ( B ). Results are calculated as fluorescence intensity normalized for α-tubulin or β-actin and expressed in percentages of LINCL cells as means±S.D. ( n =3). *: significantly different from LINCL cells with P <0.05 as determined by a Student's t test.
Figure Legend Snippet: ( A ) Mitochondrial population abundance was analysed in LINCL cells pre-incubated with (LINCL+TPP-1) or without (LINCL) recombinant TPP-1. Cells were then stained with 100 nM MitoTracker Green for 30 min and fluorescence was detected by flow cytometry as described in the Material and methods section. Results are expressed as MitoTracker Green Fluorescence MCFR calculated from autofluorescence signals measured for corresponding unstained cells and represent means±S.D. ( n =3). NS, non-significantly different from LINCL cells as determined by a Student's t test. ( B ) The abundance of HADHA, β-subunit of ATPsynthase and mt-HSP70 was determined by Western blot analysis performed on 15 μg of clear cell lysates prepared from LINCL cells pre-incubated (LINCL+TPP-1) or not (LINCL) with recombinant TPP-1. Equal protein loading was controlled by the immunodetection of β-actin or α-tubulin ( n =3). ( C ) Quantification of the abundance of β-subunit of ATP synthase (grey columns) HADHA (white columns), and mt-HSP70 (black columns) on blots presented in ( B ). Results are calculated as fluorescence intensity normalized for α-tubulin or β-actin and expressed in percentages of LINCL cells as means±S.D. ( n =3). *: significantly different from LINCL cells with P <0.05 as determined by a Student's t test.

Techniques Used: Incubation, Recombinant, Staining, Fluorescence, Flow Cytometry, Western Blot, Immunodetection


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GOW-MAC Inc thermal conductivity detector
Thermal Conductivity Detector, supplied by GOW-MAC Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thermal conductivity detector/product/GOW-MAC Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
thermal conductivity detector - by Bioz Stars, 2024-07
86/100 stars

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