7 ethoxyresorufin er  (Millipore)


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    Structured Review

    Millipore 7 ethoxyresorufin er
    Confirmation of the CYP1A1 inhibitory properties of Ulva fasciata extract by (a) Dixon plot and (b) Y-intercept of the Linewaver-Burk plot versus inhibitor's concentration. Each plot was obtained from independent reactions containing the desired concentration of <t>ethoxyresorufin</t> (0.31–5.00 μ M), 1 pM Supersome protein, 50 mM NADPH, and different concentrations of the extract in a final volume of 200 μ L. Each reaction was followed for 10 min, with the fluorescence signal being recorded every 15 s. Each point in (a) represents the mean ± SD obtained from three independent experiments. Slope of each plot in (a) was obtained and plotted versus the inverse of the concentration of ethoxyresorufin (b).
    7 Ethoxyresorufin Er, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7 ethoxyresorufin er/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7 ethoxyresorufin er - by Bioz Stars, 2020-08
    93/100 stars

    Images

    1) Product Images from "Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects"

    Article Title: Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2015/520598

    Confirmation of the CYP1A1 inhibitory properties of Ulva fasciata extract by (a) Dixon plot and (b) Y-intercept of the Linewaver-Burk plot versus inhibitor's concentration. Each plot was obtained from independent reactions containing the desired concentration of ethoxyresorufin (0.31–5.00 μ M), 1 pM Supersome protein, 50 mM NADPH, and different concentrations of the extract in a final volume of 200 μ L. Each reaction was followed for 10 min, with the fluorescence signal being recorded every 15 s. Each point in (a) represents the mean ± SD obtained from three independent experiments. Slope of each plot in (a) was obtained and plotted versus the inverse of the concentration of ethoxyresorufin (b).
    Figure Legend Snippet: Confirmation of the CYP1A1 inhibitory properties of Ulva fasciata extract by (a) Dixon plot and (b) Y-intercept of the Linewaver-Burk plot versus inhibitor's concentration. Each plot was obtained from independent reactions containing the desired concentration of ethoxyresorufin (0.31–5.00 μ M), 1 pM Supersome protein, 50 mM NADPH, and different concentrations of the extract in a final volume of 200 μ L. Each reaction was followed for 10 min, with the fluorescence signal being recorded every 15 s. Each point in (a) represents the mean ± SD obtained from three independent experiments. Slope of each plot in (a) was obtained and plotted versus the inverse of the concentration of ethoxyresorufin (b).

    Techniques Used: Concentration Assay, Fluorescence

    2) Product Images from "From mRNA Expression of Drug Disposition Genes to In Vivo Assessment of CYP-Mediated Biotransformation during Zebrafish Embryonic and Larval Development"

    Article Title: From mRNA Expression of Drug Disposition Genes to In Vivo Assessment of CYP-Mediated Biotransformation during Zebrafish Embryonic and Larval Development

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19123976

    Integrated density of resorufin in the trunk region of intact zebrafish embryos and larvae at different time points during zebrafish development between 7 h post-fertilization (hpf) and 14 d post-fertilization (dpf) after incubation with benzyloxy-methyl-resorufin (BOMR) ( a ) and 7-ethoxyresorufin (ER) ( b ). At 7 hpf ( b ), integrated density of resorufin was determined in the whole embryo. Each bar represents the mean of three biological replicates ± standard deviation (S.D.). The horizontal dotted line represents the lower limit of quantification (LLOQ). In graph ( a , b ), developmental stages with values below the LLOQ were excluded from statistical analysis. In graph ( a ), no statistically significant differences ( p > 0.05) were detected between the developmental stages that showed values above the LLOQ. The mean corrected integrated density value for 50 hpf was below zero (indicated by *). In graph ( b ), significant differences ( p ≤ 0.05) between age groups are indicated by different letters (A, B and C): integrated density of resorufin was significantly higher at 7 and 26 hpf compared to the other developmental stages ( p = 0.050 for all comparisons). Moreover, resorufin formation at 7 hpf was significantly higher than at 26 hpf ( p = 0.050).
    Figure Legend Snippet: Integrated density of resorufin in the trunk region of intact zebrafish embryos and larvae at different time points during zebrafish development between 7 h post-fertilization (hpf) and 14 d post-fertilization (dpf) after incubation with benzyloxy-methyl-resorufin (BOMR) ( a ) and 7-ethoxyresorufin (ER) ( b ). At 7 hpf ( b ), integrated density of resorufin was determined in the whole embryo. Each bar represents the mean of three biological replicates ± standard deviation (S.D.). The horizontal dotted line represents the lower limit of quantification (LLOQ). In graph ( a , b ), developmental stages with values below the LLOQ were excluded from statistical analysis. In graph ( a ), no statistically significant differences ( p > 0.05) were detected between the developmental stages that showed values above the LLOQ. The mean corrected integrated density value for 50 hpf was below zero (indicated by *). In graph ( b ), significant differences ( p ≤ 0.05) between age groups are indicated by different letters (A, B and C): integrated density of resorufin was significantly higher at 7 and 26 hpf compared to the other developmental stages ( p = 0.050 for all comparisons). Moreover, resorufin formation at 7 hpf was significantly higher than at 26 hpf ( p = 0.050).

    Techniques Used: Incubation, Standard Deviation

    3) Product Images from "From mRNA Expression of Drug Disposition Genes to In Vivo Assessment of CYP-Mediated Biotransformation during Zebrafish Embryonic and Larval Development"

    Article Title: From mRNA Expression of Drug Disposition Genes to In Vivo Assessment of CYP-Mediated Biotransformation during Zebrafish Embryonic and Larval Development

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19123976

    Description of region of interest used for the quantitative and qualitative analysis of resorufin formation in zebrafish embryos and larvae at 7 h post-fertilization (hpf) ( a ), 26 hpf ( b , c ), 50 hpf ( d , e ), 74 hpf ( f , g ), 98 hpf ( h , i ), 122 hpf ( j , k ), 9 d post-fertilization (dpf) ( l , m ) and 14 dpf ( n , o ) after exposure to benzyloxy-methyl-resorufin (BOMR) or 7-ethoxyresorufin (ER). The yellow frame indicates the region of interest in the embryo or larva. Since for most embryos/larvae the complete trunk region did not fit within one image, pictures of anterior and posterior trunk were taken separately. For the quantitative analysis of resorufin formation in each embryo/larva, average pixel intensities of anterior and posterior trunk images were combined. Figure 7 ( a ) shows a vegetal pole view of the embryo. In Figure 7 ( b–o ) lateral views of the anterior and posterior part of the trunk region are shown. Scale bar: 200 µm; ( b , c ): anterior top and dorsal right; ( d–o ): anterior left and dorsal top.
    Figure Legend Snippet: Description of region of interest used for the quantitative and qualitative analysis of resorufin formation in zebrafish embryos and larvae at 7 h post-fertilization (hpf) ( a ), 26 hpf ( b , c ), 50 hpf ( d , e ), 74 hpf ( f , g ), 98 hpf ( h , i ), 122 hpf ( j , k ), 9 d post-fertilization (dpf) ( l , m ) and 14 dpf ( n , o ) after exposure to benzyloxy-methyl-resorufin (BOMR) or 7-ethoxyresorufin (ER). The yellow frame indicates the region of interest in the embryo or larva. Since for most embryos/larvae the complete trunk region did not fit within one image, pictures of anterior and posterior trunk were taken separately. For the quantitative analysis of resorufin formation in each embryo/larva, average pixel intensities of anterior and posterior trunk images were combined. Figure 7 ( a ) shows a vegetal pole view of the embryo. In Figure 7 ( b–o ) lateral views of the anterior and posterior part of the trunk region are shown. Scale bar: 200 µm; ( b , c ): anterior top and dorsal right; ( d–o ): anterior left and dorsal top.

    Techniques Used:

    Integrated density of resorufin in the trunk region of intact zebrafish embryos and larvae at different time points during zebrafish development between 7 h post-fertilization (hpf) and 14 d post-fertilization (dpf) after incubation with benzyloxy-methyl-resorufin (BOMR) ( a ) and 7-ethoxyresorufin (ER) ( b ). At 7 hpf ( b ), integrated density of resorufin was determined in the whole embryo. Each bar represents the mean of three biological replicates ± standard deviation (S.D.). The horizontal dotted line represents the lower limit of quantification (LLOQ). In graph ( a , b ), developmental stages with values below the LLOQ were excluded from statistical analysis. In graph ( a ), no statistically significant differences ( p > 0.05) were detected between the developmental stages that showed values above the LLOQ. The mean corrected integrated density value for 50 hpf was below zero (indicated by *). In graph ( b ), significant differences ( p ≤ 0.05) between age groups are indicated by different letters (A, B and C): integrated density of resorufin was significantly higher at 7 and 26 hpf compared to the other developmental stages ( p = 0.050 for all comparisons). Moreover, resorufin formation at 7 hpf was significantly higher than at 26 hpf ( p = 0.050).
    Figure Legend Snippet: Integrated density of resorufin in the trunk region of intact zebrafish embryos and larvae at different time points during zebrafish development between 7 h post-fertilization (hpf) and 14 d post-fertilization (dpf) after incubation with benzyloxy-methyl-resorufin (BOMR) ( a ) and 7-ethoxyresorufin (ER) ( b ). At 7 hpf ( b ), integrated density of resorufin was determined in the whole embryo. Each bar represents the mean of three biological replicates ± standard deviation (S.D.). The horizontal dotted line represents the lower limit of quantification (LLOQ). In graph ( a , b ), developmental stages with values below the LLOQ were excluded from statistical analysis. In graph ( a ), no statistically significant differences ( p > 0.05) were detected between the developmental stages that showed values above the LLOQ. The mean corrected integrated density value for 50 hpf was below zero (indicated by *). In graph ( b ), significant differences ( p ≤ 0.05) between age groups are indicated by different letters (A, B and C): integrated density of resorufin was significantly higher at 7 and 26 hpf compared to the other developmental stages ( p = 0.050 for all comparisons). Moreover, resorufin formation at 7 hpf was significantly higher than at 26 hpf ( p = 0.050).

    Techniques Used: Incubation, Standard Deviation

    Localization of biotransformation of 7-ethoxyresorufin (ER) in the trunk region of intact zebrafish embryos and larvae at 26 h post-fertilization (hpf) ( b , c ), 50 hpf ( d , e ), 74 hpf ( f , g ), 98 hpf ( h , i ), 122 hpf ( j , k ) and 14 d post-fertilization (dpf) ( l , m ). At 7 hpf ( a ), qualitative analysis of resorufin formation was performed in the whole embryo. The stage of 9 dpf was excluded from the figure since resorufin formation could not be localized due to ventral position of the larvae. Pictures show one embryo/larva out of six used in the study, i.e., three biological replicates with two embryos/larvae per replicate, for each developmental stage. Figure ( a ) shows a vegetal pole view of the embryo. In Figure 5 ( b – m ) lateral views of the anterior and posterior part of the trunk region are shown. The organs in which resorufin had been formed are indicated with a two-letter combination. Since the hatching gland and otic vesicle do not belong to the trunk region, resorufin formation in the respective organs is mentioned separately. S.B.: swim bladder. Scale bar: 200 µm; ( b , c ): anterior top and dorsal right; ( d – m ): anterior left and dorsal top.
    Figure Legend Snippet: Localization of biotransformation of 7-ethoxyresorufin (ER) in the trunk region of intact zebrafish embryos and larvae at 26 h post-fertilization (hpf) ( b , c ), 50 hpf ( d , e ), 74 hpf ( f , g ), 98 hpf ( h , i ), 122 hpf ( j , k ) and 14 d post-fertilization (dpf) ( l , m ). At 7 hpf ( a ), qualitative analysis of resorufin formation was performed in the whole embryo. The stage of 9 dpf was excluded from the figure since resorufin formation could not be localized due to ventral position of the larvae. Pictures show one embryo/larva out of six used in the study, i.e., three biological replicates with two embryos/larvae per replicate, for each developmental stage. Figure ( a ) shows a vegetal pole view of the embryo. In Figure 5 ( b – m ) lateral views of the anterior and posterior part of the trunk region are shown. The organs in which resorufin had been formed are indicated with a two-letter combination. Since the hatching gland and otic vesicle do not belong to the trunk region, resorufin formation in the respective organs is mentioned separately. S.B.: swim bladder. Scale bar: 200 µm; ( b , c ): anterior top and dorsal right; ( d – m ): anterior left and dorsal top.

    Techniques Used:

    Related Articles

    Fluorescence In Situ Hybridization:

    Article Title: From mRNA Expression of Drug Disposition Genes to In Vivo Assessment of CYP-Mediated Biotransformation during Zebrafish Embryonic and Larval Development
    Article Snippet: .. Zebrafish embryos/larvae incubated with 1.7 µM 7-ethoxyresorufin (ER) (Resorufin ethyl ether, Sigma-Aldrich)—a CYP1-specific substrate—in fish medium were used as positive control since positive results have been described in literature [ , ]. .. Each embryo/larva was transferred in 150 µL of fish medium to a well of a black 96-Well Cell Imaging Plate with clear 25 µm film bottom (Eppendorf Cell Imaging Plates, 0030741013, Eppendorf, Hamburg, Germany).

    Incubation:

    Article Title: From mRNA Expression of Drug Disposition Genes to In Vivo Assessment of CYP-Mediated Biotransformation during Zebrafish Embryonic and Larval Development
    Article Snippet: .. Zebrafish embryos/larvae incubated with 1.7 µM 7-ethoxyresorufin (ER) (Resorufin ethyl ether, Sigma-Aldrich)—a CYP1-specific substrate—in fish medium were used as positive control since positive results have been described in literature [ , ]. .. Each embryo/larva was transferred in 150 µL of fish medium to a well of a black 96-Well Cell Imaging Plate with clear 25 µm film bottom (Eppendorf Cell Imaging Plates, 0030741013, Eppendorf, Hamburg, Germany).

    Article Title: p23 co-chaperone protects the aryl hydrocarbon receptor from degradation in mouse and human cell lines
    Article Snippet: .. After 6 h for Hepa1c1c7 and Hep3B cells or 16 h for HeLa cells, the cells were washed once with 0.5 ml of fresh media, followed by incubation with 0.5 ml of fresh media containing 5 μM resorufin ethyl ether (Sigma, St. Louis, MO) and 10 μM dicumarol (Sigma, St. Louis, MO). .. After 30 min, 200 μl of the media was transferred from each well into a 96-well plate and the fluorescence was measured using a Berthold Tri-Star LB941 plate reader (excitation at 544nm and emission at 590nm).

    other:

    Article Title: GPER is involved in the regulation of the estrogen-metabolizing CYP1B1 enzyme in breast cancer
    Article Snippet: Reagents 17β-Estradiol (E2), salicylamide (2-hydroxybenzamide), resorufin (7-hydroxy-3H-phenoxazin-3-one) and resorufin ethyl ether (7-ethoxy-3H-phenoxazin-3-one) were purchased from Sigma-Aldrich (Milan, Italy).

    Article Title: Multiphoton spectral analysis of benzo[a]pyrene uptake and metabolism in breast epithelial cell lines
    Article Snippet: Culture media, Dulbecco's phosphate buffered saline (PBS), Janus green, BaP, horse serum, doxorubicin, resorufin ethyl ether and 3,3'-methylene-bis(4-hydroxycoumarin) (dicumarol) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).

    Activity Assay:

    Article Title: The Henna pigment Lawsone activates the Aryl Hydrocarbon Receptor and impacts skin homeostasis
    Article Snippet: .. Briefly after 48 h of stimulation with AhR activators, 4 µM resorufin ethyl ether (EROD, Sigma-Aldrich) and 10 µM dicoumarol (Sigma-Aldrich) were added to HEK culture for 1 h and activity measured with the Fluoroskan Ascent Microplate Fluorometer (Thermo Labsystem). .. The activity was corrected to the amount of protein measured by Bradford assay and normalized to the vehicle control (DMSO).

    Positive Control:

    Article Title: From mRNA Expression of Drug Disposition Genes to In Vivo Assessment of CYP-Mediated Biotransformation during Zebrafish Embryonic and Larval Development
    Article Snippet: .. Zebrafish embryos/larvae incubated with 1.7 µM 7-ethoxyresorufin (ER) (Resorufin ethyl ether, Sigma-Aldrich)—a CYP1-specific substrate—in fish medium were used as positive control since positive results have been described in literature [ , ]. .. Each embryo/larva was transferred in 150 µL of fish medium to a well of a black 96-Well Cell Imaging Plate with clear 25 µm film bottom (Eppendorf Cell Imaging Plates, 0030741013, Eppendorf, Hamburg, Germany).

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    Millipore polyclonal rabbit anti rat cyp 1a1 antibody
    DEP exposure increased <t>CYP</t> <t>1A1</t> in LV. Representative immunohistochemistry of cytochrome P -450 (Cyp) 1A1 after 5 wk of vehicle ( A ) or DEP exposure ( B ). Darker areas represent positive Cyp 1A1 immunostaining.
    Polyclonal Rabbit Anti Rat Cyp 1a1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti rat cyp 1a1 antibody/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti rat cyp 1a1 antibody - by Bioz Stars, 2020-08
    85/100 stars
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    Image Search Results


    DEP exposure increased CYP 1A1 in LV. Representative immunohistochemistry of cytochrome P -450 (Cyp) 1A1 after 5 wk of vehicle ( A ) or DEP exposure ( B ). Darker areas represent positive Cyp 1A1 immunostaining.

    Journal: Journal of Applied Physiology

    Article Title: Exposure to diesel exhaust particulates induces cardiac dysfunction and remodeling

    doi: 10.1152/japplphysiol.00343.2013

    Figure Lengend Snippet: DEP exposure increased CYP 1A1 in LV. Representative immunohistochemistry of cytochrome P -450 (Cyp) 1A1 after 5 wk of vehicle ( A ) or DEP exposure ( B ). Darker areas represent positive Cyp 1A1 immunostaining.

    Article Snippet: Sections were blocked with 4% bovine serum albumin for 30 min and incubated over night with 1:4,000 diluted polyclonal rabbit anti-rat CYP 1A1 antibody (Millipore AB1247).

    Techniques: Immunohistochemistry, Immunostaining