c57bl 6j 693 cell lines  (ATCC)


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    ATCC c57bl 6j 693 cell lines
    MicroRNAs expressed in ESCs and CSCs and their relative functions.
    C57bl 6j 693 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "MicroRNAs and Stem-like Properties: The Complex Regulation Underlying Stemness Maintenance and Cancer Development"

    Article Title: MicroRNAs and Stem-like Properties: The Complex Regulation Underlying Stemness Maintenance and Cancer Development

    Journal: Biomolecules

    doi: 10.3390/biom11081074

    MicroRNAs expressed in ESCs and CSCs and their relative functions.
    Figure Legend Snippet: MicroRNAs expressed in ESCs and CSCs and their relative functions.

    Techniques Used: Isolation, Modification, Over Expression, FACS, Biomarker Assay, Expressing, Inhibition, Binding Assay, Knock-Out, Migration, Activation Assay

    peg 693 pseudomonas corrugata fig  (ATCC)


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    ATCC peg 693 pseudomonas corrugata fig
    Peg 693 Pseudomonas Corrugata Fig, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    in vivo selection 679 1093 693 97276 140  (ATCC)


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    ATCC in vivo selection 679 1093 693 97276 140
    In Vivo Selection 679 1093 693 97276 140, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bone 693 marrow  (ATCC)


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    ATCC bone 693 marrow
    Bone 693 Marrow, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    u2os 693  (ATCC)


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    ATCC u2os 693
    ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in <t>U2OS.693</t> cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.
    U2os 693, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Selection-Independent Generation of Gene Knockout Mouse Embryonic Stem Cells Using Zinc-Finger Nucleases"

    Article Title: Selection-Independent Generation of Gene Knockout Mouse Embryonic Stem Cells Using Zinc-Finger Nucleases

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0028911

    ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in U2OS.693 cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.
    Figure Legend Snippet: ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in U2OS.693 cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.

    Techniques Used: Knock-Out, Non-Homologous End Joining, Sequencing, Stable Transfection, Transfection, Expressing, Flow Cytometry, Standard Deviation, Cotransfection, Plasmid Preparation

    rm144 28 281 693 tgc cct ggc gca aat ttg atcc gct aga gga gat cag atg gta gtg catg  (ATCC)


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    ATCC rm144 28 281 693 tgc cct ggc gca aat ttg atcc gct aga gga gat cag atg gta gtg catg
    Rm144 28 281 693 Tgc Cct Ggc Gca Aat Ttg Atcc Gct Aga Gga Gat Cag Atg Gta Gtg Catg, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c57bl 6j 693 cell lines  (ATCC)


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    ATCC c57bl 6j 693 cell lines
    MicroRNAs expressed in ESCs and CSCs and their relative functions.
    C57bl 6j 693 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "MicroRNAs and Stem-like Properties: The Complex Regulation Underlying Stemness Maintenance and Cancer Development"

    Article Title: MicroRNAs and Stem-like Properties: The Complex Regulation Underlying Stemness Maintenance and Cancer Development

    Journal: Biomolecules

    doi: 10.3390/biom11081074

    MicroRNAs expressed in ESCs and CSCs and their relative functions.
    Figure Legend Snippet: MicroRNAs expressed in ESCs and CSCs and their relative functions.

    Techniques Used: Isolation, Modification, Over Expression, FACS, Biomarker Assay, Expressing, Inhibition, Binding Assay, Knock-Out, Migration, Activation Assay

    u s pto application no 62 693  (ATCC)


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    ATCC u s pto application no 62 693
    U S Pto Application No 62 693, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    in vivo selection 679 1093 693 97276 140  (ATCC)


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    ATCC in vivo selection 679 1093 693 97276 140
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    fusobacterium necrophorum 693 subsp necrophorum atcc 25286  (ATCC)


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    ATCC fusobacterium necrophorum 693 subsp necrophorum atcc 25286
    Fusobacterium Necrophorum 693 Subsp Necrophorum Atcc 25286, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lactobacillus gasseri 693 atcc 33323  (ATCC)


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    ATCC lactobacillus gasseri 693 atcc 33323
    Lactobacillus Gasseri 693 Atcc 33323, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC c57bl 6j 693 cell lines
    MicroRNAs expressed in ESCs and CSCs and their relative functions.
    C57bl 6j 693 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MicroRNAs expressed in ESCs and CSCs and their relative functions.
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    ATCC in vivo selection 679 1093 693 97276 140
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    In Vivo Selection 679 1093 693 97276 140, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC bone 693 marrow
    MicroRNAs expressed in ESCs and CSCs and their relative functions.
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    ATCC u2os 693
    ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in <t>U2OS.693</t> cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.
    U2os 693, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC rm144 28 281 693 tgc cct ggc gca aat ttg atcc gct aga gga gat cag atg gta gtg catg
    ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in <t>U2OS.693</t> cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.
    Rm144 28 281 693 Tgc Cct Ggc Gca Aat Ttg Atcc Gct Aga Gga Gat Cag Atg Gta Gtg Catg, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC u s pto application no 62 693
    ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in <t>U2OS.693</t> cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.
    U S Pto Application No 62 693, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC fusobacterium necrophorum 693 subsp necrophorum atcc 25286
    ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in <t>U2OS.693</t> cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.
    Fusobacterium Necrophorum 693 Subsp Necrophorum Atcc 25286, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC lactobacillus gasseri 693 atcc 33323
    ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in <t>U2OS.693</t> cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.
    Lactobacillus Gasseri 693 Atcc 33323, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MicroRNAs expressed in ESCs and CSCs and their relative functions.

    Journal: Biomolecules

    Article Title: MicroRNAs and Stem-like Properties: The Complex Regulation Underlying Stemness Maintenance and Cancer Development

    doi: 10.3390/biom11081074

    Figure Lengend Snippet: MicroRNAs expressed in ESCs and CSCs and their relative functions.

    Article Snippet: miR-30 , Together with let-7 , mir-125 , and mir-9 , it downregulates the expression of the RNA-binding protein LIN28, directly binding its 3′-UTR [ ]. , R1 and C57BL/6J-693 cell lines. , ATCC/The Jackson Laboratory. , The mechanism acting in ESCs is reversed in the LIN28-positive human breast cancer cell line, where LIN28 downregulates miR-30 , let-7 , miR-125 , and miR-9 . This could be responsible for LIN28 reactivation in a cancer context [ ]. , .

    Techniques: Isolation, Modification, Over Expression, FACS, Biomarker Assay, Expressing, Inhibition, Binding Assay, Knock-Out, Migration, Activation Assay

    ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in U2OS.693 cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.

    Journal: PLoS ONE

    Article Title: Selection-Independent Generation of Gene Knockout Mouse Embryonic Stem Cells Using Zinc-Finger Nucleases

    doi: 10.1371/journal.pone.0028911

    Figure Lengend Snippet: ( A ) Schematic of ZFN-mediated knockout. A ZFN pair (ZFN R and ZFN L ) designed to target position 502 in the EGFP gene (E502) creates a DNA double-strand break that is sealed by the error-prone non-homologous end-joining (NHEJ) pathway and hence leads to disruption of the coding sequence. ( B ) Dose-dependent gene disruption in U2OS.693 cells. U2OS.693 cells that stably express a destabilized EGFP were transfected with increasing amounts of E502-specific ZFN expression vectors (75–1200 ng). The percentage of EGFP-negative cells was determined 6 days post-transfection by flow cytometry (n = 3; indicated is average and standard deviation). E502-WT, ZFN with wild-type FokI domain; E502-OH, ZFN with obligate heterodimeric FokI domain. ( C ) ZFN expression levels. After co-transfection of HEK293T cells with ZFN expression vectors and pEGFP, cell lysates were probed with antibodies against the HA tag and EGFP. Amount of transfected ZFN plasmids was 75 ng, 300 ng, and 1200 ng. NT, non-transfected cells. ( D ) Kinetics of EGFP knockout. The graph displays the percentage of EGFP-negative cells (see B) from day 1 to day 6 post-transfection for two vector amounts (n = 3; indicated is average and standard deviation). WT, E502-WT; OH, E502-OH.

    Article Snippet: U2OS.693 is a human osteosarcoma based cell line (ATCC® #HTB-96™) that expresses a destabilized EGFP .

    Techniques: Knock-Out, Non-Homologous End Joining, Sequencing, Stable Transfection, Transfection, Expressing, Flow Cytometry, Standard Deviation, Cotransfection, Plasmid Preparation