Journal: EBioMedicine

Article Title: Pharmaco-transcriptomic correlation analysis reveals novel responsive signatures to HDAC inhibitors and identifies Dasatinib as a synergistic interactor in small-cell lung cancer

doi: 10.1016/j.ebiom.2021.103457

Figure Lengend Snippet: The effects of combined HDACi and Dasatinib on SCLC cells. A, Flow cytometry-based analyses of apoptosis (Annexin V positive cells; left) and lipid reactive oxygen species (ROS; right) assayed on SCLC cells (GLC-15) treated for 48h with Dasatinib (0.5 μM) and Vorinostat (HDACi; 1 μM), alone or in combination. The corresponding quantifications (n=3) were shown on the right. *p < 0.05, **p < 0.01, ****p < 0.0001 by ANOVA test. NS, non-significance. B, Small–cell lung cancer (SCLC) cells (GLC-15) were treated for 72h with combined Vorinostat (HDACi; 1 μM) and Dasatinib (0.5 μM) with or without 2.5 mM N-acetyl-L-cysteine (NAC). Cells were seeded 1,000 cells per well in 96-well plates. ****p < 0.0001 (n=3) by ANOVA test. NS, non-significance. C, Flow cytometry-based analyses of apoptosis (Annexin V positive cells; left) and lipid reactive oxygen species (ROS; right) assayed on SCLC cells (GLC-15) treated for 24h with Dasatinib (0.5 μM) and Vorinostat (HDACi; 1 μM), alone or in combination. The assay was repeated twice.

Article Snippet: SCLC (DMS-114 [ATCC Cat# CRL-2066, RRID:CVCL_1174], DMS-53 [ATCC Cat# CRL-2062, RRID:CVCL_1177], GLC-15 [KCB Cat# KCB 90028YJ, RRID:CVCL_6904]) and NSCLC cell lines (H1650 [KCLB Cat# 91650, RRID:CVCL_1483], H4006 [ATCC Cat# CRL-2871, RRID:CVCL_1269], H1792 [ATCC Cat# CRL-5895, RRID:CVCL_1495], H1944 [ATCC Cat# CRL-5907, RRID:CVCL_1508]) were cultured in RPMI-1640 (Sigma-Aldrich, R8758), supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Sigma-Aldrich) at 37 °C in a humid incubator with 5% CO2.

Techniques: Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: A Survey of Antimicrobial Resistance Determinants in Category A Select Agents, Exempt Strains, and Near-Neighbor Species

doi: 10.3390/ijms21051669

Figure Lengend Snippet: Microarray-detected AMR genes in Yersinia spp. strains.

Article Snippet: AMC TB4 , ATCC 6904; YE1022 , YERS008 , , .

Techniques: Microarray

Journal: International Journal of Molecular Sciences

Article Title: A Survey of Antimicrobial Resistance Determinants in Category A Select Agents, Exempt Strains, and Near-Neighbor Species

doi: 10.3390/ijms21051669

Figure Lengend Snippet: GyrA melt temperatures and clusters for Yersinia spp. strains analyzed by HRMA.

Article Snippet: AMC TB4 , ATCC 6904; YE1022 , YERS008 , , .

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Survey of Antimicrobial Resistance Determinants in Category A Select Agents, Exempt Strains, and Near-Neighbor Species

doi: 10.3390/ijms21051669

Figure Lengend Snippet: Other Yersinia spp. strains used in this study.

Article Snippet: AMC TB4 , ATCC 6904; YE1022 , YERS008 , , .

Techniques:

Journal: Medical Journal, Armed Forces India

Article Title: Speciation of fungi using real time PCR with molecular beacons: Can we solve the enigma of diagnosis of invasive fungal disease?

doi: 10.1016/j.mjafi.2017.12.003

Figure Lengend Snippet: Reference set of melting temperatures (Tm°C) generated using the four molecular beacons following amplification of DNA from standard fungal strains. Beacon PFC11 gave a TM with all strains tested while PFA21 and PYCT41 gave adequate difference in Tm to enable speciation. Beacon PFM3 gave Tm with only zygomycetes.

Article Snippet: The top of each panel shows the meting curves while the bottom half shows the melting peaks. panel a: beacon PFC11, panel b: beacon PFA21, panel c: beacon PFM3 (zygomycetes), panel d: PYCT41 (yeast). table ft1 table-wrap mode="anchored" t5 caption a7 ATCC no. Fungal strains Molecular beacon PFC11 PFA21 PFM3 PYCT41 ATCC 204304 Aspergillus flavus 64.26 70.38 – 82.23 ATCC 293 Aspergillus fumigatus 69.59 74 – 64.93 ATCC 9508 Aspergillus niger 64.17 69.92 – 64.93 Clinical isolate Aspergillus terreus 64.12 70.47 – 82.8 Clinical isolate Aspergillus nidulans 64.21 70.34 – 81.96 Clinical isolate Aspergillus glaucus 63.75 70.2 – 46 Clinical isolate Mucor 49.66 46.44 59.03 61.72 Clinical isolate Rhizopus 63.9 46.61 58.59 61.49 ATCC 90028 Candida albicans 65.19 70.34 – 45.63 ATCC 750 Candida tropicalis 65.19 70.31 – 62.47 ATCC 6258 Candida krusei 65.33 70.38 – – ATCC 22019 Candida parapsilosis 65.23 70.31 – 41.19 ATCC 6258 Candida guillermondii 64.75 70.25 – – ATCC 208821 Cryptococcus neoformans 62 68.91 – 61 Clinical isolate Trichosporon asahii 61.09 69.04 – 61.72 Clinical isolate Penicillium spp.

Techniques: Generated, Amplification

Journal: Medical Journal, Armed Forces India

Article Title: Speciation of fungi using real time PCR with molecular beacons: Can we solve the enigma of diagnosis of invasive fungal disease?

doi: 10.1016/j.mjafi.2017.12.003

Figure Lengend Snippet: Melting temperatures (Tm°C) of the beacons generated after PCR on clinical samples. The fungus was identified by matching these with the reference set of Tm in .

Article Snippet: The top of each panel shows the meting curves while the bottom half shows the melting peaks. panel a: beacon PFC11, panel b: beacon PFA21, panel c: beacon PFM3 (zygomycetes), panel d: PYCT41 (yeast). table ft1 table-wrap mode="anchored" t5 caption a7 ATCC no. Fungal strains Molecular beacon PFC11 PFA21 PFM3 PYCT41 ATCC 204304 Aspergillus flavus 64.26 70.38 – 82.23 ATCC 293 Aspergillus fumigatus 69.59 74 – 64.93 ATCC 9508 Aspergillus niger 64.17 69.92 – 64.93 Clinical isolate Aspergillus terreus 64.12 70.47 – 82.8 Clinical isolate Aspergillus nidulans 64.21 70.34 – 81.96 Clinical isolate Aspergillus glaucus 63.75 70.2 – 46 Clinical isolate Mucor 49.66 46.44 59.03 61.72 Clinical isolate Rhizopus 63.9 46.61 58.59 61.49 ATCC 90028 Candida albicans 65.19 70.34 – 45.63 ATCC 750 Candida tropicalis 65.19 70.31 – 62.47 ATCC 6258 Candida krusei 65.33 70.38 – – ATCC 22019 Candida parapsilosis 65.23 70.31 – 41.19 ATCC 6258 Candida guillermondii 64.75 70.25 – – ATCC 208821 Cryptococcus neoformans 62 68.91 – 61 Clinical isolate Trichosporon asahii 61.09 69.04 – 61.72 Clinical isolate Penicillium spp.

Techniques: Generated