wnv  (ATCC)


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    ATCC wnv
    Wnv, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli strain scpm o b 6860  (ATCC)


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    ATCC e coli strain scpm o b 6860
    E Coli Strain Scpm O B 6860, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli strain scpm o b 6860  (ATCC)


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    ATCC e coli strain scpm o b 6860
    Electron microscopic images of <t>E.</t> <t>coli</t> SCPM-O-B-9427.
    E Coli Strain Scpm O B 6860, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Hetero-Pathogenic O181:H4 EAHEC Strain of Sequence Type ST678 Associated with Hemolytic–Uremic Syndrome in Schoolchildren in Russia"

    Article Title: Hetero-Pathogenic O181:H4 EAHEC Strain of Sequence Type ST678 Associated with Hemolytic–Uremic Syndrome in Schoolchildren in Russia

    Journal: Microorganisms

    doi: 10.3390/microorganisms11071771

    Electron microscopic images of E. coli SCPM-O-B-9427.
    Figure Legend Snippet: Electron microscopic images of E. coli SCPM-O-B-9427.

    Techniques Used:

    Cytotoxicity of E. coli strains SCPM-O-B-9427 of O181:H4 serotype, K12 (C600), and SCPM-O-B-6860 of O104:H4 serotype for Vero cells: ( a ) with mitomycin C induction and ( b ) without mitomycin C induction; 1, 1:2 dilution; 2, 1:4 dilution; 3, 1:8 dilution; 4, 1:16 dilution; 5, 1:32 dilution; 6, 1:64 dilution.
    Figure Legend Snippet: Cytotoxicity of E. coli strains SCPM-O-B-9427 of O181:H4 serotype, K12 (C600), and SCPM-O-B-6860 of O104:H4 serotype for Vero cells: ( a ) with mitomycin C induction and ( b ) without mitomycin C induction; 1, 1:2 dilution; 2, 1:4 dilution; 3, 1:8 dilution; 4, 1:16 dilution; 5, 1:32 dilution; 6, 1:64 dilution.

    Techniques Used:

    wnv  (ATCC)


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    ATCC wnv
    Wnv, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b lata lmg 6860  (ATCC)


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    ATCC b lata lmg 6860
    Taxon K isolates included in the present study.
    B Lata Lmg 6860, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Burkholderia cepacia Complex Taxon K: Where to Split?"

    Article Title: Burkholderia cepacia Complex Taxon K: Where to Split?

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2020.01594

    Taxon K isolates included in the present study.
    Figure Legend Snippet: Taxon K isolates included in the present study.

    Techniques Used:

    Genomic characteristics of Burkholderia genomes included in the present study.
    Figure Legend Snippet: Genomic characteristics of Burkholderia genomes included in the present study.

    Techniques Used:

    west nile virus wnv rnac  (ATCC)


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    ATCC west nile virus wnv rnac
    West Nile Virus Wnv Rnac, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    west nile virus wnv  (ATCC)


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    ATCC west nile virus wnv
    West Nile Virus Wnv, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    west nile virus  (ATCC)


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    ATCC west nile virus
    West Nile Virus, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    wnv env  (ATCC)


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    ATCC wnv env
    ( a ) Immunoblot analysis of the expression of Slfn11 in A172 cells stably expressing shRNAs directed against Slfn11 (A172-KD) or a scrambled (A172-SCR) RNA sequences and A172-KD cells engineered to re-express Slfn11 (A172-BC). α-tubulin was detected as a loading control. ( b ) <t>WNV</t> replication in A172-derived cells. A172-SCR (open triangles), A172-KD (filled diamonds) and A172-BC (filled squares) cells were infected with WNV (MOI 0.1) and viral replication was determined by quantification of the viral titer in the cell supernatant at different hours post-infection by plaque assay. Statistically significant differences were calculated with repeated measures ANOVA and Tukey-Kramer post-hoc tests and they are indicated with asterisks. Mean and standard deviation values represent the variability of the viral titer found in triplicate plaque assays of samples from 8 independent infection experiments performed in different days with different viral preparations. ( c ) Expression of <t>WNV</t> <t>Env</t> in cells infected at MOI 0.1 evaluated by immunoblot 40 hrs after infection. α-tubulin was detected as a loading control. These results are representative of 3 independent infections. ( d ) Expression of WNV Env (red) as detected by indirect immunofluorescence analysis of cells infected at a MOI of 1 48 hrs post-infection. Nuclei were labeled with Hoechst (blue).
    Wnv Env, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Schlafen 11 Restricts Flavivirus Replication"

    Article Title: Schlafen 11 Restricts Flavivirus Replication

    Journal: bioRxiv

    doi: 10.1101/434563

    ( a ) Immunoblot analysis of the expression of Slfn11 in A172 cells stably expressing shRNAs directed against Slfn11 (A172-KD) or a scrambled (A172-SCR) RNA sequences and A172-KD cells engineered to re-express Slfn11 (A172-BC). α-tubulin was detected as a loading control. ( b ) WNV replication in A172-derived cells. A172-SCR (open triangles), A172-KD (filled diamonds) and A172-BC (filled squares) cells were infected with WNV (MOI 0.1) and viral replication was determined by quantification of the viral titer in the cell supernatant at different hours post-infection by plaque assay. Statistically significant differences were calculated with repeated measures ANOVA and Tukey-Kramer post-hoc tests and they are indicated with asterisks. Mean and standard deviation values represent the variability of the viral titer found in triplicate plaque assays of samples from 8 independent infection experiments performed in different days with different viral preparations. ( c ) Expression of WNV Env in cells infected at MOI 0.1 evaluated by immunoblot 40 hrs after infection. α-tubulin was detected as a loading control. These results are representative of 3 independent infections. ( d ) Expression of WNV Env (red) as detected by indirect immunofluorescence analysis of cells infected at a MOI of 1 48 hrs post-infection. Nuclei were labeled with Hoechst (blue).
    Figure Legend Snippet: ( a ) Immunoblot analysis of the expression of Slfn11 in A172 cells stably expressing shRNAs directed against Slfn11 (A172-KD) or a scrambled (A172-SCR) RNA sequences and A172-KD cells engineered to re-express Slfn11 (A172-BC). α-tubulin was detected as a loading control. ( b ) WNV replication in A172-derived cells. A172-SCR (open triangles), A172-KD (filled diamonds) and A172-BC (filled squares) cells were infected with WNV (MOI 0.1) and viral replication was determined by quantification of the viral titer in the cell supernatant at different hours post-infection by plaque assay. Statistically significant differences were calculated with repeated measures ANOVA and Tukey-Kramer post-hoc tests and they are indicated with asterisks. Mean and standard deviation values represent the variability of the viral titer found in triplicate plaque assays of samples from 8 independent infection experiments performed in different days with different viral preparations. ( c ) Expression of WNV Env in cells infected at MOI 0.1 evaluated by immunoblot 40 hrs after infection. α-tubulin was detected as a loading control. These results are representative of 3 independent infections. ( d ) Expression of WNV Env (red) as detected by indirect immunofluorescence analysis of cells infected at a MOI of 1 48 hrs post-infection. Nuclei were labeled with Hoechst (blue).

    Techniques Used: Western Blot, Expressing, Stable Transfection, Derivative Assay, Infection, Plaque Assay, Standard Deviation, Immunofluorescence, Labeling

    west nile virus  (ATCC)


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    ATCC west nile virus
    West Nile Virus, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    west nile virus  (ATCC)


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    ATCC west nile virus
    West Nile Virus, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC wnv
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    ATCC e coli strain scpm o b 6860
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    ATCC b lata lmg 6860
    Taxon K isolates included in the present study.
    B Lata Lmg 6860, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC west nile virus wnv rnac
    Taxon K isolates included in the present study.
    West Nile Virus Wnv Rnac, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC west nile virus wnv
    Taxon K isolates included in the present study.
    West Nile Virus Wnv, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC west nile virus
    Taxon K isolates included in the present study.
    West Nile Virus, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC wnv env
    ( a ) Immunoblot analysis of the expression of Slfn11 in A172 cells stably expressing shRNAs directed against Slfn11 (A172-KD) or a scrambled (A172-SCR) RNA sequences and A172-KD cells engineered to re-express Slfn11 (A172-BC). α-tubulin was detected as a loading control. ( b ) <t>WNV</t> replication in A172-derived cells. A172-SCR (open triangles), A172-KD (filled diamonds) and A172-BC (filled squares) cells were infected with WNV (MOI 0.1) and viral replication was determined by quantification of the viral titer in the cell supernatant at different hours post-infection by plaque assay. Statistically significant differences were calculated with repeated measures ANOVA and Tukey-Kramer post-hoc tests and they are indicated with asterisks. Mean and standard deviation values represent the variability of the viral titer found in triplicate plaque assays of samples from 8 independent infection experiments performed in different days with different viral preparations. ( c ) Expression of <t>WNV</t> <t>Env</t> in cells infected at MOI 0.1 evaluated by immunoblot 40 hrs after infection. α-tubulin was detected as a loading control. These results are representative of 3 independent infections. ( d ) Expression of WNV Env (red) as detected by indirect immunofluorescence analysis of cells infected at a MOI of 1 48 hrs post-infection. Nuclei were labeled with Hoechst (blue).
    Wnv Env, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Taxon K isolates included in the present study.

    Journal: Frontiers in Microbiology

    Article Title: Burkholderia cepacia Complex Taxon K: Where to Split?

    doi: 10.3389/fmicb.2020.01594

    Figure Lengend Snippet: Taxon K isolates included in the present study.

    Article Snippet: B. lata LMG 6860 , ATCC 17771; NCIB9091 , 1404 , Soil (Trinidad and Tobago, 1960) , NCIMB.

    Techniques:

    Genomic characteristics of Burkholderia genomes included in the present study.

    Journal: Frontiers in Microbiology

    Article Title: Burkholderia cepacia Complex Taxon K: Where to Split?

    doi: 10.3389/fmicb.2020.01594

    Figure Lengend Snippet: Genomic characteristics of Burkholderia genomes included in the present study.

    Article Snippet: B. lata LMG 6860 , ATCC 17771; NCIB9091 , 1404 , Soil (Trinidad and Tobago, 1960) , NCIMB.

    Techniques:

    ( a ) Immunoblot analysis of the expression of Slfn11 in A172 cells stably expressing shRNAs directed against Slfn11 (A172-KD) or a scrambled (A172-SCR) RNA sequences and A172-KD cells engineered to re-express Slfn11 (A172-BC). α-tubulin was detected as a loading control. ( b ) WNV replication in A172-derived cells. A172-SCR (open triangles), A172-KD (filled diamonds) and A172-BC (filled squares) cells were infected with WNV (MOI 0.1) and viral replication was determined by quantification of the viral titer in the cell supernatant at different hours post-infection by plaque assay. Statistically significant differences were calculated with repeated measures ANOVA and Tukey-Kramer post-hoc tests and they are indicated with asterisks. Mean and standard deviation values represent the variability of the viral titer found in triplicate plaque assays of samples from 8 independent infection experiments performed in different days with different viral preparations. ( c ) Expression of WNV Env in cells infected at MOI 0.1 evaluated by immunoblot 40 hrs after infection. α-tubulin was detected as a loading control. These results are representative of 3 independent infections. ( d ) Expression of WNV Env (red) as detected by indirect immunofluorescence analysis of cells infected at a MOI of 1 48 hrs post-infection. Nuclei were labeled with Hoechst (blue).

    Journal: bioRxiv

    Article Title: Schlafen 11 Restricts Flavivirus Replication

    doi: 10.1101/434563

    Figure Lengend Snippet: ( a ) Immunoblot analysis of the expression of Slfn11 in A172 cells stably expressing shRNAs directed against Slfn11 (A172-KD) or a scrambled (A172-SCR) RNA sequences and A172-KD cells engineered to re-express Slfn11 (A172-BC). α-tubulin was detected as a loading control. ( b ) WNV replication in A172-derived cells. A172-SCR (open triangles), A172-KD (filled diamonds) and A172-BC (filled squares) cells were infected with WNV (MOI 0.1) and viral replication was determined by quantification of the viral titer in the cell supernatant at different hours post-infection by plaque assay. Statistically significant differences were calculated with repeated measures ANOVA and Tukey-Kramer post-hoc tests and they are indicated with asterisks. Mean and standard deviation values represent the variability of the viral titer found in triplicate plaque assays of samples from 8 independent infection experiments performed in different days with different viral preparations. ( c ) Expression of WNV Env in cells infected at MOI 0.1 evaluated by immunoblot 40 hrs after infection. α-tubulin was detected as a loading control. These results are representative of 3 independent infections. ( d ) Expression of WNV Env (red) as detected by indirect immunofluorescence analysis of cells infected at a MOI of 1 48 hrs post-infection. Nuclei were labeled with Hoechst (blue).

    Article Snippet: Infected cells were fixed and permeabilized with Cytofix/Cytoperm buffer (BD Biosciences, Cat#554714) at 24hrs and 48hrs post-infection and then stained with an anti-Flavivirus group antigen monoclonal antibody that recognizes WNV Env (ATCC, clone D1-4G2-4-15, 1/200) for 2hrs at room temperature.

    Techniques: Western Blot, Expressing, Stable Transfection, Derivative Assay, Infection, Plaque Assay, Standard Deviation, Immunofluorescence, Labeling