dose dependent manner  (ATCC)

Journal: Microbiology Spectrum

Article Title: Genomic Degeneration and Reduction in the Fish Pathogen Mycobacterium shottsii

doi: 10.1128/spectrum.01158-21

Figure Lengend Snippet: Multicopy insertion sequences (IS) in M. shottsii M175 ^a

Article Snippet: IS Mysh 01 has lower similarity (68.3%) to single-copy transposases from M. marinum strains CCUG 20998 and ATCC 927 and M. tuberculosis group bacteria (IS Mt 1-family).

Techniques: Plasmid Preparation

Journal: Microbiology Spectrum

Article Title: Genomic Degeneration and Reduction in the Fish Pathogen Mycobacterium shottsii

doi: 10.1128/spectrum.01158-21

Figure Lengend Snippet: Synteny of ESX-2 locus (orange) of M. tuberculosis H37Rv ( Rv3884c to Rv3895c ) relative to M. shottsii M175 and several M. marinum strains where ESX-2 is present. M. marinum strains ATCC 927 and M with missing ESX-2 locus are shown for comparison. Flanking CDS of M. marinum ATCC 927 ( MMRN_RS28480 to MMRN_RS28490 ) and M ( MMAR_RS27415 to MMAR_RS27425 ) are shown in red. Partial deletion of eccA2 ortholog is shown in brown ( MMRN_RS28481 and MMAR_RS27420 , respectively). Direction of arrows represents strand of gene; genes are drawn to scale. M. shottsii M175 partial ESX-2 locus is flanked by IS Mysh 01 elements (green).

Article Snippet: IS Mysh 01 has lower similarity (68.3%) to single-copy transposases from M. marinum strains CCUG 20998 and ATCC 927 and M. tuberculosis group bacteria (IS Mt 1-family).

Techniques:

Journal: Microorganisms

Article Title: Marine Alkaloid 2,2-Bis(6-bromo-3-indolyl) Ethylamine and Its Synthetic Derivatives Inhibit Microbial Biofilms Formation and Disaggregate Developed Biofilms

doi: 10.3390/microorganisms7020028

Figure Lengend Snippet: Minimum Inhibitory Concentration (MIC) values (µg/mL) of the tested compounds against selected microbial strains, assessed by broth microdilution method according to the Performance Standards for Antimicrobial Susceptibility Testing (CLSI Document M100-S2, 2013). Gentamicin and fluconazole were used as internal controls.

Article Snippet: The lowest concentration (8 µg/mL), tested against E. coli ATCC 35218 and S. aureus ATCC 43387, provoked 68.3 and 82.2% of biofilm formation inhibition, respectively; the same concentration induced only 40.0% of K. pneumoniae 6/4 biofilm formation inhibition, reaching 83.7% of inhibition with 16 µg/mL (2× MIC value).

Techniques: Concentration Assay

Journal: Microorganisms

Article Title: Marine Alkaloid 2,2-Bis(6-bromo-3-indolyl) Ethylamine and Its Synthetic Derivatives Inhibit Microbial Biofilms Formation and Disaggregate Developed Biofilms

doi: 10.3390/microorganisms7020028

Figure Lengend Snippet: Biofilm formation inhibition exerted by compounds 1 , 3 , 4 , and 8 . Each molecule was added at its relative MIC and 2× MIC (µg/mL) values during the biofilm formation of the different pathogens; the inhibition percentages were determined after spectrophotometer reader at 570 nm.

Article Snippet: The lowest concentration (8 µg/mL), tested against E. coli ATCC 35218 and S. aureus ATCC 43387, provoked 68.3 and 82.2% of biofilm formation inhibition, respectively; the same concentration induced only 40.0% of K. pneumoniae 6/4 biofilm formation inhibition, reaching 83.7% of inhibition with 16 µg/mL (2× MIC value).

Techniques: Inhibition, Spectrophotometry

Journal: Microorganisms

Article Title: Marine Alkaloid 2,2-Bis(6-bromo-3-indolyl) Ethylamine and Its Synthetic Derivatives Inhibit Microbial Biofilms Formation and Disaggregate Developed Biofilms

doi: 10.3390/microorganisms7020028

Figure Lengend Snippet: Assessment of viable cells harvested from biofilms of E. coli ATCC 35218, S. aureus ATCC 43387, K. pneumoniae 6/4 and C. albicans ATCC 10231 after 30 min of treatment with compounds 1 , 3 , 4 , and 8 at their relative MIC values. Data represent the mean ± SD of cfu/mL values obtained in three independent experiments performed in duplicate. Asterisks represent values statistically significant (* p < 0.05; ** p < 0.01; *** p < 0.001) as compared to the related controls.

Article Snippet: The lowest concentration (8 µg/mL), tested against E. coli ATCC 35218 and S. aureus ATCC 43387, provoked 68.3 and 82.2% of biofilm formation inhibition, respectively; the same concentration induced only 40.0% of K. pneumoniae 6/4 biofilm formation inhibition, reaching 83.7% of inhibition with 16 µg/mL (2× MIC value).

Techniques:

Journal: Microorganisms

Article Title: Marine Alkaloid 2,2-Bis(6-bromo-3-indolyl) Ethylamine and Its Synthetic Derivatives Inhibit Microbial Biofilms Formation and Disaggregate Developed Biofilms

doi: 10.3390/microorganisms7020028

Figure Lengend Snippet: Disaggregating efficacy of compounds 1 , 3 , 4 , and 8 at their relative MIC values, against biofilms of E. coli ATCC 35218, S. aureus ATCC 43387, K. pneumoniae 6/4 and C. albicans ATCC 10231, as assessed by spectrophotometer reader (OD 570nm ) after 30 min of contact. Data represent the mean ± SD obtained in three independent experiments performed in duplicate. Asterisks represent values statistically significant (* p < 0.05; ** p < 0.01) as compared to the related controls.

Article Snippet: The lowest concentration (8 µg/mL), tested against E. coli ATCC 35218 and S. aureus ATCC 43387, provoked 68.3 and 82.2% of biofilm formation inhibition, respectively; the same concentration induced only 40.0% of K. pneumoniae 6/4 biofilm formation inhibition, reaching 83.7% of inhibition with 16 µg/mL (2× MIC value).

Techniques: Spectrophotometry

Journal: Microorganisms

Article Title: Marine Alkaloid 2,2-Bis(6-bromo-3-indolyl) Ethylamine and Its Synthetic Derivatives Inhibit Microbial Biofilms Formation and Disaggregate Developed Biofilms

doi: 10.3390/microorganisms7020028

Figure Lengend Snippet: Percentages of biofilm eradication activity assessed for E. coli ATCC 35218, S. aureus ATCC 43387, K. pneumoniae 6/4 and C. albicans ATCC 10231 after 30 min of contact with compounds 1 , 3 , 4 , and 8 at their relative MIC (µg/mL) values. Percentages were determined after spectrophotometer reader (OD 570nm ).

Article Snippet: The lowest concentration (8 µg/mL), tested against E. coli ATCC 35218 and S. aureus ATCC 43387, provoked 68.3 and 82.2% of biofilm formation inhibition, respectively; the same concentration induced only 40.0% of K. pneumoniae 6/4 biofilm formation inhibition, reaching 83.7% of inhibition with 16 µg/mL (2× MIC value).

Techniques: Activity Assay, Spectrophotometry