Journal: Scientific Reports

Article Title: Schisandrin B targets CDK4/6 to suppress proliferation and enhance radiosensitivity in nasopharyngeal carcinoma by inducing cell cycle arrest

doi: 10.1038/s41598-025-92992-9

Figure Lengend Snippet: Cell cycle contributes to Sch B-induced NPC cell growth inhibition. ( A ) The top 20 enriched KEGG pathways of differentially expressed genes of HONE-1_Sch B/HONE-1. ( B ) The top 20 enriched KEGG pathways of differentially expressed genes of HONE-1_Sch B/ NP69_Sch B – . ( C ) The heat-map analysis of 122 cell-cycle related gene expression in HONE-1, HONE-1_Sch B and NP69. ( D ) The heatmap of gene expression of CDK2, CDK4, CDK6, Cyclin D1, Cyclin E1.

Article Snippet: The PVDF membrane was sealed with 5% skim milk at room temperature for 1 h, then incubated overnight at 4 °C with an antibody CDK4 (Proteintech, Illinois, USA, Cat. No. 11026-1-AP, 1:2000), CDK6 (CST, Cat. No. 13331S, 1:1000), Cyclin D1 (CST, Cat. No. 2978S, 1:1000), and GAPDH (CST, Cat. No. 5174S, 1:8000).

Techniques: Inhibition, Gene Expression

Journal: Scientific Reports

Article Title: Schisandrin B targets CDK4/6 to suppress proliferation and enhance radiosensitivity in nasopharyngeal carcinoma by inducing cell cycle arrest

doi: 10.1038/s41598-025-92992-9

Figure Lengend Snippet: Sch B induces cell-cycle arrest at G1 phase of HONE-1 and CNE-1 cells, but not normal nasopharyngeal epithelial cell NP69. ( A – C ) Flow cytometry of cell cycle distribution in HONE-1, CNE-1 and NP69 cells treated with 0, 10, 20, 30, 40 μM Sch B. ( D ) Immunoblot analysis of CDK4, CDK6, Cyclin D1, protein levels in HONE-1, CNE-1 and NP69 cells treated with 0, 20, 30 μM Sch B. GAPDH was used as a loading control.

Article Snippet: The PVDF membrane was sealed with 5% skim milk at room temperature for 1 h, then incubated overnight at 4 °C with an antibody CDK4 (Proteintech, Illinois, USA, Cat. No. 11026-1-AP, 1:2000), CDK6 (CST, Cat. No. 13331S, 1:1000), Cyclin D1 (CST, Cat. No. 2978S, 1:1000), and GAPDH (CST, Cat. No. 5174S, 1:8000).

Techniques: Flow Cytometry, Western Blot, Control

Journal: Scientific Reports

Article Title: Schisandrin B targets CDK4/6 to suppress proliferation and enhance radiosensitivity in nasopharyngeal carcinoma by inducing cell cycle arrest

doi: 10.1038/s41598-025-92992-9

Figure Lengend Snippet: Sch B targets CDK4/6 to induce cell-cycle arresting at G1 phase. ( A ) Cellular thermal shift assay for HONE-1 and CNE-1 cells treated with 0 and 30 μM Sch B. ( B ) Molecular docking of Sch B binding to CDK4 and CDK6.

Article Snippet: The PVDF membrane was sealed with 5% skim milk at room temperature for 1 h, then incubated overnight at 4 °C with an antibody CDK4 (Proteintech, Illinois, USA, Cat. No. 11026-1-AP, 1:2000), CDK6 (CST, Cat. No. 13331S, 1:1000), Cyclin D1 (CST, Cat. No. 2978S, 1:1000), and GAPDH (CST, Cat. No. 5174S, 1:8000).

Techniques: Thermal Shift Assay, Binding Assay

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Tasquinimod promotes the sensitivity of ovarian cancer cells to cisplatin by down-regulating the HDAC4/p21 pathway

doi: 10.4196/kjpp.24.132

Figure Lengend Snippet: Primer sequences

Article Snippet: The membranes were then incubated overnight at 4°C with primary antibodies including HDAC4 (#7628, 1:1,000, CST), Bcl-2 (12789-1-AP, 1:2,000, Proteintech), cleaved-Caspase-3 (#9661, 1:1,000, CST), p21 (Human, P21 fusion protein Ag0368, 10355-1-AP, 1:50, Proteintech), cyclin D1 (60186-1-Ig, 1:2,000, Proteintech), CDK4 (11026-1-AP, 1:3,000, Proteintech), and β -actin (66009-1-Ig, 1:5,000, Proteintech).

Techniques:

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Tasquinimod promotes the sensitivity of ovarian cancer cells to cisplatin by down-regulating the HDAC4/p21 pathway

doi: 10.4196/kjpp.24.132

Figure Lengend Snippet: (A, B) qRT-PCR analysis of p21, cyclin D1, and CDK4 expression levels in SKOV3/DDP cells and A2780/DDP cells after Tasquinimod treatment. (C, D) Immunofluorescence analysis of p21, cyclin D1, and CDK4 expression levels in SKOV3/DDP cells and A2780/DDP cells after Tasquinimod treatment. Scale bars represent 25 μm. (E) CCK8 assays to verify the cell viability in SKOV3/DDP cells and A2780/DDP cells after Tasquinimod treatment. (F) Flow cytometry detection of SKOV3/DDP cell and A2780/DDP cells cycle after Tasquinimod treatment. All experiments were independently repeated six times, Turkey’s test and one-way ANOVA were used to analyze and calculate statistical significance. Values are presented as mean ± SD. OC, ovarian cancer; CCK8, cell counting kit-8; NC, negative control; IOD, integrated optical density. *p < 0.05 vs . SKOV3/DDP or A2780/DDP group, # p < 0.05 vs . SKOV3/DDP + Tas + NC or A2780/DDP + Tas + NC group, & p < 0.05 vs . SKOV3/DDP + Tas or A2780/DDP + Tas group.

Article Snippet: The membranes were then incubated overnight at 4°C with primary antibodies including HDAC4 (#7628, 1:1,000, CST), Bcl-2 (12789-1-AP, 1:2,000, Proteintech), cleaved-Caspase-3 (#9661, 1:1,000, CST), p21 (Human, P21 fusion protein Ag0368, 10355-1-AP, 1:50, Proteintech), cyclin D1 (60186-1-Ig, 1:2,000, Proteintech), CDK4 (11026-1-AP, 1:3,000, Proteintech), and β -actin (66009-1-Ig, 1:5,000, Proteintech).

Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Flow Cytometry, Cell Counting, Negative Control

Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

Article Title: Tasquinimod promotes the sensitivity of ovarian cancer cells to cisplatin by down-regulating the HDAC4/p21 pathway

doi: 10.4196/kjpp.24.132

Figure Lengend Snippet: (A–C) Tumor volume and weight were measured and compared in mice after treatment with Tasquinimod combined with cisplatin. (D) Immunofluorescence analysis of HDAC4 expression levels in tumor cells in mice. (E) Western blot analysis of HDAC4, p21, cyclin D1, and CDK4 expression levels in mouse tumor tissues. (F) Flow cytometry analysis of apoptosis in tumor tissues in mice. All experiments were independently repeated three times. Tukey’s test and one-way ANOVA were used to analyze statistical significance. Values are presented as mean ± SD. OC, ovarian cancer; HDAC4, histone deacetylase 4; IOD, integrated optical density. *p < 0.05 vs . SKOV3 or A2780 group, # p < 0.05 vs . SKOV3/DDP or A2780/DDP group.

Article Snippet: The membranes were then incubated overnight at 4°C with primary antibodies including HDAC4 (#7628, 1:1,000, CST), Bcl-2 (12789-1-AP, 1:2,000, Proteintech), cleaved-Caspase-3 (#9661, 1:1,000, CST), p21 (Human, P21 fusion protein Ag0368, 10355-1-AP, 1:50, Proteintech), cyclin D1 (60186-1-Ig, 1:2,000, Proteintech), CDK4 (11026-1-AP, 1:3,000, Proteintech), and β -actin (66009-1-Ig, 1:5,000, Proteintech).

Techniques: Immunofluorescence, Expressing, Western Blot, Flow Cytometry, Histone Deacetylase Assay